Lisa Elviri

University of Parma

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Maria Careri

University of Parma

Ruggero Bettini

University of Parma

Luciano Marchiò

University of Parma

Irene Bassanetti

Chiesi Foundation

Annalisa Bianchera

University of Parma

Carlo Bergonzi

University of Parma

Giulia Remaggi

University of Parma

Chiara Bignardi

University of Parma

A. Mangia

Azienda Unità Sanitaria Locale Piacenza

Claudio Mucchino

University of Parma

Cooperative Institutions

University of Parma

245

University of Milan

National Research Council

Istituti di Ricovero e Cura a Carattere Scientifico

Centre National de la Recherche Scientifique

University of Bologna

University of Padua

University of Modena and Reggio Emilia

Universidade de São Paulo

National Interuniversity Consortium of Materials Science and Technology

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Element-tagged immunoassay with inductively coupled plasma mass spectrometry for multianalyte detection

Analytical and Bioanalytical Chemistry (2008)

Maria Careri Lisa Elviri Alessandro Mangia

10.1007/s00216-008-2419-8

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Citations (41)

Fugitive Acromegaly: A Historical, Clinical, and Translational Perspective

Frontiers of hormone research (2024)

Roberto Toni Fulvio Barbaro Giusy Di Conza Lisa Elviri S. Mosca

The term 'fugitive acromegaly' was introduced by the neurosurgeons Bailey and Cushing in 1928 to describe subjects manifesting signs and symptoms of somatotroph hyperfunction with pituitary insufficiency. Currently, it identifies patients with subtle acromegalic dysmorphisms and inconsistent hormonal profile, possibly presenting only with hyperprolactinemia and related clinical symptoms. Patients have rapidly growing, locally invasive, relapsing pituitary macrotumors that can be classified as either acidophil stem cell tumors (ASCTs) or sparsely granulated somatotroph tumors (SGSTs), both of PIT1-lineage. ASCTs also express estrogen receptor (ER)α, show predominant prolactin (PRL) release, and less abundantly, growth hormone (GH). In contrast, SGSTs have moderately increased GH and IGF1 levels, but rarely PRL increase. ASCTs often present resistance to dopamine agonists, and long-acting somatostatin analogs are used. In contrast, SGSTs are often resistant to somatostatin analogues and instead are treated with the GH receptor antagonist pegvisomant. Differential diagnosis includes mammosomatotroph, mixed GH-/PRL-secreting, immature PIT1-lineage, and densely granulated somatotroph tumors. Studies in ER-sensitive rat tumoral mammosomatotroph cells (GH3, GH4C1) suggest that overexpression of chaperones in immature PIT1-/ER-expressing progenitors induces posttranscriptional conformational changes to tumor suppressors of the ERα and aryl hydrocarbon receptor pathways, like AIP, leading to the development of aggressive pituitary tumors like those causing fugitive acromegaly.

Translational Research

10.1159/000539942

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Photodegradation of Pharmaceutical Pollutants: New Photocatalytic Systems Based on 3D Printed Scaffold-Supported Ag/TiO2 Nanocomposite

Catalysts (2022)

Laura Bergamonti Claudia Graiff Carlo Bergonzi Marianna Potenza Cinzia Reverberi

Due to the release of active pharmaceutical compounds in wastewater and their persistence in the environment, dangerous consequences can develop in the aquatic and terrestrial organisms. Chitosan/Ag/TiO2 3D printed scaffolds, at different Ag nanoparticle concentrations (10, 100, 1000 ppm) are investigated here as promising materials for photocatalytic degradation under the UV–Vis irradiation of pharmaceutical compounds in wastewater. As target drugs, amoxicillin, paracetamol and their 1:1 mix were selected. Ag nanoparticles increase the photocatalytic efficiency of the system based on titanium dioxide embedded in the chitosan scaffold: in the presence of Chitosan/Ag100/TiO2, the selected pharmaceuticals (PhCs), monitored by UV–Vis spectroscopy, are completely removed in about 2 h. The photodegradation products of the PhCs were identified by Liquid Chromatography–Mass Spectroscopy and assessed for their toxicological impact on six different bacterial strains: no antibacterial activity was found towards the tested strains. This new system based on Ag/TiO2 supported on 3D chitosan scaffolds may represent an effective strategy to reduce wastewater pollution by emerging contaminants.

Photodegradation

Titanium Dioxide

10.3390/catal12060580

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Citations (10)

CCDC 280048: Experimental Crystal Structure Determination

The Cambridge Structural Database (2006)

R. Pattacini Laurel R. Barbieri A. Stercoli D. Cauzzi C. Graiff

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

10.5517/cc9ddtw

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CCDC 773133: Experimental Crystal Structure Determination

The Cambridge Structural Database (2011)

Irene Bassanetti Marcello Gennari Luciano Marchiò M. Terenghi Lisa Elviri

10.5517/cctyhs0

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CCDC 792833: Experimental Crystal Structure Determination

The Cambridge Structural Database (2011)

Saverio Tardito Irene Bassanetti Chiara Bignardi Lisa Elviri M. Tegoni

10.5517/ccvm08q

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Cultured Horse Articular Chondrocytes in 3D-Printed Chitosan Scaffold With Hyaluronic Acid and Platelet Lysate

Frontiers in Veterinary Science (2021)

Elena De Angelis Roberta Saleri P. Martelli Lisa Elviri Annalisa Bianchera

Three-dimensional (3D) printing has gained popularity in tissue engineering and in the field of cartilage regeneration. This is due to its potential to generate scaffolds with spatial variation of cell distribution or mechanical properties, built with a variety of materials that can mimic complex tissue architecture. In the present study, horse articular chondrocytes were cultured for 2 and 4 weeks in 3D-printed chitosan (CH)-based scaffolds prepared with or without hyaluronic acid and in the presence of fetal bovine serum (FBS) or platelet lysate (PL). These 3D culture systems were analyzed in terms of their capability to maintain chondrocyte differentiation in vitro . This was achieved by evaluating cell morphology, immunohistochemistry (IHC), gene expression of relevant cartilage markers ( collagen type II, aggrecan, and Sox9 ), and specific markers of dedifferentiated phenotype ( collagen type I, Runx2 ). The morphological, histochemical, immunohistochemical, and molecular results demonstrated that the 3D CH scaffold is sufficiently porous to be colonized by primary chondrocytes. Thereby, it provides an optimal environment for the colonization and synthetic activity of chondrocytes during a long culture period where a higher rate of dedifferentiation can be generally observed. Enrichment with hyaluronic acid provides an optimal microenvironment for a more stable maintenance of the chondrocyte phenotype. The use of 3D CH scaffolds causes a further increase in the gene expression of most relevant ECM components when PL is added as a substitute for FBS in the medium. This indicates that the latter system enables a better maintenance of the chondrocyte phenotype, thereby highlighting a fair balance between proliferation and differentiation.

Aggrecan

Platelet lysate

Type II collagen

SOX9

10.3389/fvets.2021.671776

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Citations (8)

Rapid Assay for Analyzing Biogenic Amines in Cheese: Matrix Solid-Phase Dispersion Followed by Liquid Chromatography−Electrospray−Tandem Mass Spectrometry

Journal of Agricultural and Food Chemistry (2005)

F. Calbiani Maria Careri Lisa Elviri A. Mangia L. Pistarà

A new rapid and sensitive method based on matrix solid-phase dispersion (MSPD) followed by liquid chromatography−electrospray−tandem mass spectrometry was devised for the determination of biogenic amines at trace levels in cheese samples. The method required 0.25 g of sample, CN-bonded silica as a dispersant sorbent, and a formic acid aqueous solution/methanol mixture as an eluting solvent. Extraction recoveries from soft cheese products were calculated in the 98 ± 4−110 ± 6% range. A procedure based on solid-phase extraction was also evaluated for the extraction of these compounds in cheese. Chromatographic separation was performed using a C18 column with an aqueous ammonium acetate/methanol mixture as the mobile phase under gradient conditions. The method was validated in terms of detection limits (LOD), quantitation limits (LOQ), linearity, recovery, precision, and trueness. Results in the 0.05−0.25 mg kg-1 range were obtained for the LOD of histamine, tyramine, and β-phenylethylamine in soft cheese samples. Linearity was established over 2 orders of magnitude. Excellent precision in terms of intra-day repeatability was calculated (RSD% < 5). The applicability of the method to the determination of biogenic amines in cheese products was demonstrated. Keywords: Biogenic amines; matrix solid phase dispersion; liquid chromatography−tandem mass spectrometry

Electrospray mass spectrometry

Matrix (chemical analysis)

Tandem

10.1021/jf048037g

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Citations (64)

In Search of a Vaccine for Mouse Allergy: Significant Reduction of Mus m 1 Allergenicity by Structure-Guided Single-Point Mutations

International Archives of Allergy and Immunology (2011)

Elena Ferrari Daniela Breda Renato Longhi Luca Vangelista Clóvis R. Nakaie

Mouse urinary proteins are relevant allergens from mice urine. We used the recombinant protein Mus m 1 as an allergen model to identify if, by altering Mus m 1 architecture via single-point mutations, we could effectively modify its allergenicity.Based on structural considerations, we synthesized two single-point mutants, Mus m 1-Y120A and Mus m 1-Y120F, which were expected to harbor large structural alterations. Circular dichroism and fluorescence analysis showed significant conformational rearrangements of the aromatic side chains in the internal cavity of Mus m 1-Y120A when compared to Mus m 1-Y120F and Mus m 1. Evaluation of the allergenic potential of the recombinant molecules was performed in vitro with both immunochemical approaches and assays based on the measurement of basophil degranulation. Moreover, to assess the integrity of the T cell epitopes and as an in vitro measure of immunogenicity, we tested the reactivity of T lymphocytes from subjects allergic to mouse urine against proteins and synthetic peptides encompassing the immunodominant linear epitope containing the mutation.We found that the selected point mutation was able to modulate the protein allergenicity, and to severely impair the recognition of Mus m 1 by IgE, while T cell reactivity was fully maintained.In silico predicted, minimum selected structural modifications allowed to design one protein with reduced allergenicity and preserved immunogenicity. Structurally guided mutations can direct the design of proteins with reduced allergenicity which can be used as vaccines for a safer and more effective immunotherapy of allergic disorders.

10.1159/000327551

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Citations (22)

Complexes between Recombinant Intracellular Carriers of Vitamin A and Their Specific Ligands Investigated by Electrospray Mass Spectrometry

European Journal of Mass Spectrometry (2004)

Maria Careri Lisa Elviri I. Zagnoni Davide Cavazzini Gian Luigi Rossi

The intracellular carriers of vitamin A, cellular retinol-binding protein type I, cellular retinol-binding protein type II and cellular retinoic acid-binding protein type I are members of the intracellular lipid-binding proteins family, in which the ligand-binding cavity is located in the interior of a barrel-like structure. The dissociation constants of the specific complexes in water solutions around neutrality are very low (in the 0.1 to 10 nM range). Because of their high stability, they represent ideal systems to verify the adequacy of electrospray ionization mass spectrometry in the analysis of non-covalent protein–ligand complexes. The electrospray interface parameters were varied to detect the presence of species not present in solution but generated as artefacts during transfer of complexes from the condensed state to the gas-phase. The results clearly indicate that mass-spectrometry data reflect the situation present in solution only if the electrospray conditions are carefully selected. In particular, the values of cone voltage and temperature compatible with persistence of the complexes in the gas phase were determined for each vitamin A carrier. Lack of correlation between complex stability in solution and in the gas phase is attributable to the specific and differential effects of the two environments on protein conformation and ligand–protein interactions.

Protein ligand

Dissociation constant

10.1255/ejms.607

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Citations (3)