Julian R. Naglik

King's College London

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Bernhard Hube

Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI)

David L. Moyes

King's College London

Jonathan P. Richardson

King's College London

Stephen Challacombe

King's College London

Jemima Ho

King's College London

Sarah L. Gaffen

University of Pittsburgh

Nicole O. Ponde

King's College London

Selene Mogavero

Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI)

Celia Murciano

King's College London

Selvam Thavaraj

King's College London

Cooperative Institutions

King's College London

Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI)

Friedrich Schiller University Jena

University of Pittsburgh

University of Aberdeen

Cornell University

University College London

Guy's Hospital

National Institutes of Health

The London College

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The Mycobiome: Influencing IBD Severity

Cell Host & Microbe (2012)

David L. Moyes Julian R. Naglik

10.1016/j.chom.2012.05.009

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Citations (39)

Issue Information

Oral Diseases (2020)

Anwar R. Tappuni Stephen Challacombe Irna Sufi David Croser Giovanni Lodi

10.1111/odi.13587

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Citations (0)

Oral epithelial cells and their interactions with HIV‐1

Oral Diseases (2016)

David L. Moyes Ashraful Islam Arinder Kohli Julian R. Naglik

As the AIDS pandemic has continued, our understanding of the events that occur during the entry and infection of conventional, susceptible cells has increased dramatically, leading to the development of control therapies for HIV ‐infected individuals. However, an ongoing hole in our understanding is how HIV crosses the mucosal barriers to gain access to permissive cells, despite how important this information would be in developing successful vaccines and other preventative measures such as topical anti‐ HIV microbicides. In particular, our knowledge of the role that epithelial cells of the mucosal surfaces play in infection – both during early phases and throughout the life of an infected individual, is currently hazy at best. However, several studies in recent years suggest that HIV can bind to and traverse these mucosal epithelial cells, providing a reservoir of infection that can subsequently infect underlying permissive cells. Despite this interaction with epithelial cells, evidence suggests HIV ‐1 does not productively infect these cells, although they are capable of transferring surface‐bound and transcytosed virus to other, permissive cells. Further, there appear to be key differences between adult and infant epithelial cells in the degree to which HIV can transcytose and infect the epithelium. Thus, it is clear that, whilst not primary targets for infection and virus replication, epithelial cells play an important role in the infection cycle and improving our understanding of their interactions with HIV could potentially provide key insights necessary to develop effective preventative therapies.

Permissive

10.1111/odi.12410

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Citations (11)

Secretion of the fungal toxin candidalysin is dependent on conserved precursor peptide sequences

Nature Microbiology (2024)

Rita Müller Annika König Sabrina Groth Robert Żarnowski Corissa Visser

10.1038/s41564-024-01606-z

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Citations (2)

Candida innate immunity at the mucosa

Seminars in Cell and Developmental Biology (2018)

Jonathan P. Richardson David L. Moyes Jemima Ho Julian R. Naglik

Commensalism

10.1016/j.semcdb.2018.02.026

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Citations (60)

Pathogenicity mechanisms and host response during oral Candida albicans infections

Expert Review of Anti-infective Therapy (2014)

Betty Hebecker Julian R. Naglik Bernhard Hube Ilse D. Jacobsen

Oral candidiasis remains one of the most common forms of Candida infections and occurs if the balance between host, Candida and microbiota is disturbed, e.g., by broad spectrum antibiotics or immunosuppression. In recent years, identification of fungal factors contributing to host cell damage and new insights into host defense mechanisms have significantly extended our understanding of the pathogenesis of oral candidiasis. In this review, we will provide an overview of the pathogenicity mechanisms during oral Candida infections and discuss some approaches by which this knowledge could be transferred into therapeutic approaches.

Immunosuppression

Host factors

Host response

Pathogenesis

10.1586/14787210.2014.916210

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Citations (88)

Analysis of Epithelial Cell Responses to Microbial Pathogens

Methods in molecular biology (2021)

Spyridoula-Angeliki Nikou Jemima Ho Olivia W. Hepworth Nicole O. Ponde Ruth E. Dickenson

Bystander effect

10.1007/978-1-0716-1182-1_5

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Citations (1)

Oral and Vaginal Epithelial Cell Lines Bind and Transfer Cell-Free Infectious HIV-1 to Permissive Cells but Are Not Productively Infected

PLoS ONE (2014)

Arinder Kohli Ayesha Islam David L. Moyes Celia Murciano Chengguo Shen

The majority of HIV-1 infections worldwide are acquired via mucosal surfaces. However, unlike the vaginal mucosa, the issue of whether the oral mucosa can act as a portal of entry for HIV-1 infection remains controversial. To address potential differences with regard to the fate of HIV-1 after exposure to oral and vaginal epithelium, we utilized two epithelial cell lines representative of buccal (TR146) and pharyngeal (FaDu) sites of the oral cavity and compared them with a cell line derived from vaginal epithelium (A431) in order to determine (i) HIV-1 receptor gene and protein expression, (ii) whether HIV-1 genome integration into epithelial cells occurs, (iii) whether productive viral infection ensues, and (iv) whether infectious virus can be transferred to permissive cells. Using flow cytometry to measure captured virus by HIV-1 gp120 protein detection and western blot to detect HIV-1 p24 gag protein, we demonstrate that buccal, pharyngeal and vaginal epithelial cells capture CXCR4- and CCR5-utilising virus, probably via non-canonical receptors. Both oral and vaginal epithelial cells are able to transfer infectious virus to permissive cells either directly through cell-cell attachment or via transcytosis of HIV-1 across epithelial cells. However, HIV-1 integration, as measured by real-time PCR and presence of early gene mRNA transcripts and de novo protein production were not detected in either epithelial cell type. Importantly, both oral and vaginal epithelial cells were able to support integration and productive infection if HIV-1 entered via the endocytic pathway driven by VSV-G. Our data demonstrate that under normal conditions productive HIV-1 infection of epithelial cells leading to progeny virion production is unlikely, but that epithelial cells can act as mediators of systemic viral dissemination through attachment and transfer of HIV-1 to permissive cells.

Transcytosis

10.1371/journal.pone.0098077

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Citations (33)

Role of the fungal peptide toxin Candidalysin in macrophage damage and inflammatory response

Lydia Kasper Axel Franke Selene Mogavero Saskia J.G. Hoefs Richard A. Martin

Inflammatory response

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Citations (3)

Candida albicans HWP1 gene expression and host antibody responses in colonization and disease

Journal of Medical Microbiology (2006)

Julian R. Naglik Florentia Fostira Jasmeet Ruprai Janet F. Staab Stephen Challacombe

In vivo expression of the developmentally regulated Candida albicans hyphal wall protein 1 ( HWP1 ) gene was analysed in human subjects who were culture positive for C. albicans and had oral symptoms ( n =40) or were asymptomatic ( n =29), or had vaginal symptoms ( n =40) or were asymptomatic ( n =29). HWP1 mRNA was present regardless of symptoms, implicating hyphal and possibly pseudohyphal forms in mucosal carriage as well as disease. As expected, in control subjects without oral symptoms ( n =10) and without vaginal symptoms ( n =10) who were culture negative in oral and vaginal samples, HWP1 mRNA was not detected. However, exposure to Hwp1 in healthy culture-negative controls, as well as in oral candidiasis and asymptomatic mucosal infections, was shown by the existence of local salivary and systemic adaptive antibody responses to Hwp1. The results are consistent with a role for Hwp1 in gastrointestinal colonization as well as in mucosal symptomatic and asymptomatic infections. Overall, Hwp1 and hyphal growth forms appear to be important factors in benign and invasive interactions of C. albicans with human hosts.

10.1099/jmm.0.46737-0

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Citations (102)