A. Petrocelli

Associazione Italiana Vulvodinia Onlus

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Mirella Biava

Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani

Maria Cecília Rui Luvizotto

Universidade Estadual Paulista (Unesp)

G. Cataldi

Associazione Italiana Vulvodinia Onlus

Ana Maria Bonetti

Universidade Federal de Uberlândia

Heitor F. Ferrari

Solução Ambiental Assessoria e Consultoria

Michela Delli Guanti

Charité - Universitätsmedizin Berlin

Maria Rosaria Capobianchi

Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani

Tereza Cristina Cardoso

Universidade Estadual Paulista (Unesp)

Antonino Di

European Society of Clinical Microbiology and Infectious Diseases

Kelfala Konneh

Ministry of Health and Sanitation

Cooperative Institutions

Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani

Istituti di Ricovero e Cura a Carattere Scientifico

Center for Prevention and Treatment of Infections

Associazione Italiana Vulvodinia Onlus

University of Milan

St John of God Hospital Sierra Leone

Ministry of Health and Sanitation

Sapienza University of Rome

European Molecular Biology Laboratory

Universidade Estadual Paulista (Unesp)

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EBOLA Ag K-SeT rapid test: field evaluation in Sierra Leone

Clinical Microbiology and Infection (2017)

Francesca Colavita Mirella Biava Pascal Mertens Quentin Gilleman Céline Borlon

Ebolavirus

10.1016/j.cmi.2017.10.019

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Citations (12)

Detection of Turkey Astrovirus in Young Poults Affected with Poult Enteritis Complex in Brazil

Journal of Veterinary Medical Science (2008)

S.E.L. da Silva Ana Maria Bonetti A. Petrocelli Heitor F. Ferrari Maria Cecília Rui Luvizotto

Reverse transcription polymerase chain reaction (RT-PCR) of turkey astrovirus (TAstV) capsid and polymerase genes was applied to the bursa of Fabricius (BF), thymus (TH), spleen (SP) and cloacal swabs (CS) of young poults with "Poult enteritis complex" (PEC). The histological lesions included atrophy, lymphoid depletion, cellular infiltration and necrosis of the BF, TH and SP, respectively. The RT-PCR reactions were positive for the polymerase gene of TAstV-2 in all 100 CSs, 7 out of 10 of BFs and 10 out of 20 THs and SPs, respectively. Five out of 10 THs and SPs samples, considered to be negative by RT-PCR, were positive when specific primers designed for the TAstV-2 capsid gene were applied. This is the first description of turkey astrovirus infection presenting PEC in Latin America.

Astrovirus

Bursa of Fabricius

10.1292/jvms.70.629

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Citations (16)

Evaluation of a rapid and sensitive RT-qPCR assay for the detection of Ebola Virus

Journal of Virological Methods (2017)

Mirella Biava Francesca Colavita Anna Marzorati Denise Hage Russo Danilo Pirola

Ebolavirus

10.1016/j.jviromet.2017.11.009

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Citations (24)

Epidemiological aspects of astrovirus and coronavirus in poults in the South Eastern Region of Brazil

Brazilian Journal of Microbiology (2009)

S.E.L. da Silva Ana Maria Bonetti A. Petrocelli Heitor F. Ferrari Maria Cecília Rui Luvizotto

A survey of Turkey Coronavirus (TCoV) and Astrovirus (TAstV-2) prevalence was carried out from February to December during 2006 year in semiarid region of Brazil, from a turkey producer area, localized in South Eastern of Brazil. To asses the risk factor related to clinical material, climatic condition and type of RT-PCR applied, cloacal swabs (CS), faeces, sera, bursa of Fabricius (BF), thymus (TH) and spleen (SP) and ileum-caeca region were collected from 30-day-old poults suffering of enteritis episode characterized as poult enteritis mortality syndrome (PEMS). The PEMS clinical features were characterized by watery to foamy faeces, light brown-yellow in colour and low mortality rate. Meteorological data (rainfall and relative humidity) observed during along the study presented monthly average temperature ranging from 39.3 and 31.2ºC, precipitation in rainy season from 40 to 270.3 mm/month, and no rain during dry season. Simplex RT-PCR gave odds ratio (OR) values suggesting that ileum-caeca region is at higher chance (OR=1.9; p=0.9741) to have both viral RNA than faeces (OR=1.5; p=0.7319). However, multiplex RT-PCR showed 3.98 (p=0.89982) more chance to give positive results in faeces than CS at dry season. The major risk factors seem to be low rate of humidity and high temperatures at winter, probably responsible for spread, easily, the TCoV and TAstv-2 among the flocks. The positive results of both virus suggested that they can play an important role in enteric disorders, associated to low humidity and high temperatures frequently found in tropical countries.

Flock

Dry season

Astrovirus

Wet season

Prevalence

10.1590/s1517-83822009000200008

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Citations (19)