Michele Grego

Royal Netherlands Institute for Sea Research

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Henk Bolhuis

Royal Netherlands Institute for Sea Research

Lucas J. Stal

Royal Netherlands Institute for Sea Research

Patricia Sánchez‐Baracaldo

University of Bristol

Elliot Druce

University of Bristol

Joanne S. Boden

University of Bristol

Thorsten Bauersachs

Kiel University

Ellen C. Hopmans

Royal Netherlands Institute for Sea Research

Nicole J. Bale

Royal Netherlands Institute for Sea Research

Lorenz Schwark

Curtin University

Veronique Confurius-Guns

Royal Netherlands Institute for Sea Research

Cooperative Institutions

Royal Netherlands Institute for Sea Research

Utrecht University

University of Bristol

University of Amsterdam

Netherlands Institute of Ecology

University of Cologne

Kiel University

University of Plymouth

Brown University

Nederlands Instituut Voor Zuivel Oonderzoek

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A novel heterocyst glycolipid detected in a pelagic N2-fixing cyanobacterium of the genus Calothrix

Organic Geochemistry (2018)

Nicole J. Bale Ellen C. Hopmans Denise J C Dorhout Lucas J. Stal Michele Grego

Heterocyst

10.1016/j.orggeochem.2018.06.009

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Draft genome sequences of three filamentous cyanobacteria isolated from brackish habitats

Journal of Genomics (2021)

Joanne S. Boden Michele Grego Henk Bolhuis Patricia Sánchez‐Baracaldo

Brackish cyanobacterial genome sequences are relatively rare. Here, we report the 5.5 Mbp, 5.8 Mbp and 6.1 Mbp draft genomes of Spirulina sp. CCY15215, Leptolyngbya sp. CCY15150 and Halomicronema sp. CCY15110 isolated from coastal microbial mats on the North Sea beach of the island of Schiermonnikoog in the Netherlands. Large scale phylogenomic analyses reveal that Spirulina sp. CCY15215 is a large cell diameter cyanobacterium, whereas Leptolyngbya sp. CCY15150 and Halomicronema sp. CCY15110 are the first reported brackish genomes belonging to the LPP clade consisting primarily of Leptolyngbya, Plectonema and Phormidium spp. Further genome mining divulges that all new draft genomes contain, ggpS and ggpP, the genes responsible for synthesising glucosylglycerol (GG), a compatible solute found in moderately salt-tolerant cyanobacteria.

Marine toxin

10.7150/jgen.53678

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Temperature induced changes in the heterocyst glycolipid composition of N2 fixing heterocystous cyanobacteria

Organic Geochemistry (2014)

Thorsten Bauersachs Lucas J. Stal Michele Grego Lorenz Schwark

Heterocyst

Nostoc

Phycobiliprotein

Anabaena variabilis

Hexose

10.1016/j.orggeochem.2014.02.006

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Draft Genome Sequences of Synechococcus sp. strains CCAP1479/9, CCAP1479/10, CCAP1479/13, CCY0621, and CCY9618: Five Freshwater Syn/Pro Clade Picocyanobacteria

Journal of Genomics (2023)

Elliot Druce Michele Grego Henk Bolhuis Penny J Johnes Patricia Sánchez‐Baracaldo

Picocyanobacteria are essential primary producers in freshwaters yet little is known about their genomic diversity and ecological niches. We report here five draft genomes of freshwater picocyanobacteria: Synechococcus sp. CCAP1479/9, Synechococcus sp. CCAP1479/10, and Synechococcus sp. CCAP1479/13 isolated from Lake Windermere in the Lake District, UK; and Synechococcus sp. CCY0621 and Synechococcus sp. CCY9618 isolated from lakes in The Netherlands. Phylogenetic analysis reveals all five strains belonging to sub-cluster 5.2 of the Synechococcus and Prochlorococcus clade of Cyanobacteria. These five strains are divergent from Synechococcus elongatus, an often-used model for freshwater Synechococcus. Functional annotation revealed significant differences in the number of genes involved in the transport and metabolism of several macro-molecules between freshwater picocyanobacteria from sub-cluster 5.2 and Synechococcus elongatus, including amino acids, lipids, and carbohydrates. Comparative genomic analysis identified further differences in the presence of photosynthesis-associated proteins while gene neighbourhood comparisons revealed alternative structures of the nitrate assimilation operon nirA.

Prochlorococcus

10.7150/jgen.81013

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Circadian clock-controlled gene expression in co-cultured, mat-forming cyanobacteria

Scientific Reports (2020)

Christine Hörnlein Veronique Confurius-Guns Michele Grego Lucas J. Stal Henk Bolhuis

10.1038/s41598-020-69294-3

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Cryopreservation and recovery of a complex hypersaline microbial mat community

Cryobiology (2024)

Henk Bolhuis Michele Grego

Cryopreservation of microorganisms is an essential tool in industrial- and food applications where conservation of microbial activity and critical beneficial traits need to be guaranteed to provide a consistent product or production process. This often refers to simple, single species or low diversity assemblages in liquid cultures that can easily be revived and regrown to perform the desired process. Cryopreservation is also of essence for scientific experimentation where many environmental samples are taken in remote sampling sites and at high costs. Biobanking, or the long term preservation and potential revival of complex, structured samples come with an additional challenge related to maintaining the structure upon revival. Here we look at cryopreserving and reviving a complex photosynthesis driven microbial mat from a hypersaline ecosystem. Amplicon sequencing of the 16S and 18S ribosomal RNA gene was used to determine the community composition of bacteria and eukaryotes respectively. The tests included the use of different cryopreservative agents and different times of cryopreservation at −150 °C. Upon revival, the cryopreservatives cannot be separated from the preserved samples without disturbing the community structure, while carryover of these compounds may influence reconstitution of the communities. Indeed, although both Glycerol and Me2SO are good cryopreservatives of microbial assemblages, carryover of these compounds had a profound negative effect on the reestablishment of a functional microbial mat. Best cryopreservation and reconstitution results were obtained in the absence of a cryopreservative agent or when methanol was used.

Amplicon sequencing

10.1016/j.cryobiol.2024.104859

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