Chanyanuch Putim

Chiang Mai University

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Bordin Butr-Indr

Chiang Mai University

Ratikorn Gamngoen

Chiang Mai University

Ponrut Phunpae

Chiang Mai University

Watchara Kasinrerk

National Science and Technology Development Agency

Sorasak Intorasoot

Chiang Mai University

Chatchai Tayapiwatana

Chiang Mai University

Sittiruk Roytrakul

National Science and Technology Development Agency

Chatchai Tayapiwatana

Chiang Mai University

Usanee Anukool

Chiang Mai University

Chayada Sitthidet Tharincharoen

Chiang Mai University

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Chiang Mai University

National Science and Technology Development Agency

National Center for Genetic Engineering and Biotechnology

Ministry of Public Health

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National Centre for Genetic Resources and Biotechnology

Maharaj Nakorn Chiang Mai Hospital

Mahidol University

Tri-Service General Hospital

Konkuk University

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Secretome profile analysis of multidrug-resistant, monodrug-resistant and drug-susceptible Mycobacterium tuberculosis

Archives of Microbiology (2017)

Chanyanuch Putim Narumon Phaonakrop Janthima Jaresitthikunchai Ratikorn Gamngoen Khajornsak Tragoolpua

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Pyrazinamide

10.1007/s00203-017-1448-0

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The application of quantitative SDS-PAGE method for measurement of extracellular recombinant scFv anti-p17 in crude protein

DOAJ (DOAJ: Directory of Open Access Journals) (2013)

Kannaporn Intachai Chanyanuch Putim Watchara Kasinrerk Chatchai Tayapiwatana Bordin Butr-Indr

The quantitative determination of recombinant protein in crude protein is not commonly used because the protein is not in purified form. The Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was developed for quantitative determination of protein. This method has been use for protein quantitative determinations in many propose such as Tamm-Horsfall glycoprotein determination in kidney stone disease patient and determination of titin and nebulin in chicken meat product for meat industries. In this study, the method of quantitative determination of extracellular recombinant single-chain variable fragment anti-HIV-1 p17 proteins (scFv anti-p17) from Escherichia coli stain HB2151 by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was developed by the application of bovine serum albumin as external and internal standard. The range of detection was 1.875 - 0.234 μg. These results presented variation coefficient less than 35%. The method applied to quantitative determination of scFv anti-p17 in 19 of crude protein samples. The quantitative protein concentration results related to the enzyme-linked immunosorbent assay (ELISA) and western immunoblotting method. The quantitative SDS-PAGE method is capable for quantitative determination of known protein from crude protein extraction. This method decreased the purification step, time consuming and protein yield loss. This application is capable to apply with the other pathogenic protein markers in the future clinical applications. Bull Chiang Mai Assoc Med Sci 2013; 46(2): 107-121

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Extensively-drug resistant tuberculosis: A new strain and future challenge

DOAJ (DOAJ: Directory of Open Access Journals) (2015)

Ratikorn Gamngoen Chanyanuch Putim Bordin Burt-Indr

Extensively-Drug Resistant Tuberculosis (XDR-TB) is emerging as an even more ominous treat. XDR-TB is defined as TB that is resistant to any fluoroquinolone, and at least one of three injectable second-line drugs (capreomycin, kanamycin and amikacin), in addition to isoniazid and rifampicin. This makes XDR-TB treatment extremely complicated, if not impossible, in general treatment. In a 2006 XDR-TB outbreak in KwaZulu-Natal, South Africa, 52 of 53 people who contracted the disease died within months, It is estimated that 70% of XDR-TB patients die within a month of diagnosis. The most recent drug-resistance surveillance data issued by the WHO estimates that an average of roughly 5 percent of Multi-drug resistant tuberculosis (MDR-TB) cases are XDR-TB. Estimating the incidence of XDR-TB is extremely difficult because most laboratories are ill-equipped to detect and diagnose. To avoid the emergence of more resistant strains that may lead to almost untreatable disease, we must focus our efforts on the right management of drug resistance tuberculosis.

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A comparison of Rv0559c and Rv0560c expression in drug-resistant Mycobacterium tuberculosis in response to first-line antituberculosis drugs

Tuberculosis (2017)

Ratikorn Gamngoen Chanyanuch Putim Parichat Salee Ponrut Phunpae Bordin Butr-Indr

10.1016/j.tube.2017.11.002

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Citations (10)