The study involved the screening of fourteen isolates of Aspergillus niger for citric acid production from glucose. The study was aimed at screening and improving local strains of Aspergillus niger with potential for citric acid production. All the isolates screened produced varying amounts of citric acid, the highest was by the isolate designated CP3 which produced 12.81g/l and the least was B11 and O12 which produced 3.42g/l all after 6 days of submerged fermentation. The isolate CP3 was chosen for further studies. Strain improvement studies which was based on exposure of CP3 to ultraviolet irradiation for 10, 30 and 45 minutes increased citric acid yield to 22.20g/l (48.89% increase) by mutant M45 followed by a citric acid yield of 16.22g/l by the 30 minutes mutant (M30) and the least being M10 which produced 13.66g/l in all cases after 6 days of fermentation. The mutant (M45) also yielded higher amount of citric acid (19.64g/l) without methanol as additive compared to the parent strain (CP3) which gave a citric acid of 14.94g/l with the addition of methanol after six days (144h) of fermentation in both cases. Finding from this study showed that all the isolates have potential for citric acid production.Keywords: Isolation, Screening, Improvement, Aspergillus niger, Citric acid
Raw and differently processed roselle (Hibiscus sabdariffa) seed meal were fed as part replacement for groundnut caketo one hundred and fifty (150) Abor acre broiler chickens whose carcass characteristics were determined. The birds were randomly assigned to five dietary treatments with three replicates (ten chicks per replicate) each in a completely randomized design (CRD). In the feeding trial, raw roselle seed meal (RaRSM), roasted roselle seed meal (RaRSM), boiled roselle seed meal (BRSM) and soaked roselle seed meal (SRSM) were incorporated as part replacement for groundnut cake at 50% level of inclusion. Results obtained for cut-up parts showed no significant (P>0.05) differences except for wings of the bird fed roasted roselle seed meal was significantly (P<0.05) different and higher than those fed control, raw, boiled and soaked roselle seed meal (10.87, 7.52, 9.85 and 9.46% respectively). Results from visceral organs showed no significant (P>0.05) different but for proventriculus which exhibited significant (P<0.05) differences among the treatment means. Birds fed roasted roselle seed meal (RaRSM) had the highest value (0.80%) while those fed raw roselle seed meal (RaRSM) gave the least (0.50%). This study however, revealed that using processed roselle (Hibiscus sabdariffa) seed meal as part replacement for groundnut cake did not pose any threat to the cut-up parts and the visceral organs of broiler chickens.
One hundred and fifty (150)day-old Abor acre broiler chicks were used for this experiment toassess growth performance and carcass characteristics of broilers fed processed roselle (Hibiscus sabdariffa) seed meal were investigated. The birds were randomly assigned to five dietary treatments with three replicates (ten chicks per replicate)each in a completely randomized design (CRD). In the feeding trial, raw roselle seed meal (R RSM), roasted roselle seed meal (R RSM), boiled roselle seed meal (BRSM) and soaked roselle seed meal (SRSM) were incorporated as part replacement for groundnut cake at 50% level of inclusion.Proximate composition of the processed test ingredients and the results showed that there were no significant (P>0.05) differences among the treatment means. Raw roselle seed meal (RaRSM) gave the least crude protein while the highest was recorded in boiled roselle seed meal (BRSM). Birds fed diet without roselle seed meal inclusion (control diet) had the best final body weight and feed conversion ratio but not significantly (P>0.05) different from other treatments.Birds in treatments 2 and 4 (RaRSMand BRSM) had the least survival rate (96.67%)while the rest treatments had 100%. Cut-up parts showed no significant (P>0.05) differences exceptfor the wings of the bird fed roasted roselle seedmeal was significantly (P<0.05) different and higher than those fed control, raw, boiled and soaked roselle seed meal (10.87, 7.52, 9.85 and 9.46%, respectively). Results from visceral organs showed no significant (P>0.05) differencesexceptproventriculus which exhibited significant (P<0.05) differences among the treatment means. Proventriculus of bird fed roasted roselle seedmeal (R RSM)was highest (0.80%)while those fed raw roselle seedmeal (R RSM) gave the least (0.50%). This study however, revealed that using processed (roasted, boiled and soaked) roselle (Hibiscus sabdariffa) seed meal as part replacement for groundnut cake has no deleterious effects onthe broilerchickens.