Fabrizio Poccia

Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani

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Chiara Agrati

Istituti di Ricovero e Cura a Carattere Scientifico

Vittorio Colizzi

University of Rome Tor Vergata

Federico Martini

Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani

Gianpiero D’Offizi

Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani

Cristiana Gioia

Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani

Miroslav Malkovský

University of Wisconsin–Madison

Leopoldo Paolo Pucillo

Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani

Maria Rosaria Capobianchi

Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani

Giuseppe Ippolito

Weifang Medical University

Rita Casetti

Istituti di Ricovero e Cura a Carattere Scientifico

Cooperative Institutions

Istituti di Ricovero e Cura a Carattere Scientifico

126

Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani

University of Rome Tor Vergata

Sapienza University of Rome

Center for Prevention and Treatment of Infections

Istituto Superiore di Sanità

University of Palermo

Fondazione Santa Lucia

Inserm

National Research Council

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Different Cytokine Production and Effector/Memory Dynamics of αβ+ or γδ+ T-Cell Subsets in the Peripheral Blood of Patients with Active Pulmonary Tuberculosis

International Journal of Immunopathology and Pharmacology (2003)

Cristiana Gioia Chiara Agrati Delia Goletti Donatella Vincenti Silvia Carrara

Immunity to M.tuberculosis (MTB) infection consists of interactions between various T-cell subsets that control the infection and prevent further reactivation. We analysed the effector/memory T-cell dynamics and cytokines production in the peripheral blood of patients with pulmonary tuberculosis (TB). We observed that the frequency of CD4 + T-cell effectors was significantly increased during active TB, confirming a major role of this T-cell subset in TB immunity. Pre-terminally differentiated CD8 + T-lymphocytes were increased in the peripheral blood as well. In contrast, we observed a reduced number of effector mycobacteria-reactive γδ + T-lymphocytes with a specific defects in reacting to mycobacterial nonpeptidic antigens, suggesting that this innate response is rapidly lost during TB infection. Nevertheless, the frequency of γδ + T-cells effectors in TB patients was higher than the αβ + T-cell response to peptide from MTB-ESAT-6 protein and quantitatively similar to PPD reactivity. Thus, αβ + and γδ + T-cell differentiation and function are differently triggered by active TB infection.

Memory T cell

10.1177/039463200301600310

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Citations (29)

Phenotypic and functional properties of γδ T Cells from patients with Guillain Barré syndrome

Journal of Neuroimmunology (2000)

Giovanna Borsellino Fabrizio Poccia Roberta Placido Daniela Tramonti Giorgio Mancino

C-type lectin

10.1016/s0165-5728(99)00165-4

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Citations (17)

Expansion of pre-terminally differentiated CD8 T cells in chronic HIV-positive patients presenting a rapid viral rebound during structured treatment interruption

AIDS (2002)

Gianpiero D’Offizi Carla Montesano Chiara Agrati Cristiana Gioia Massimo Amicosante

Objective: The influence of structured treatment interruption on effector/memory CD8 T cell dynamics was analysed in chronic HIV-infected patients showing a rapid or delayed viral rebound. Design: Structured treatment interruption consisted of at least one month of discontinuation, followed by highly active antiretroviral therapy (HAART) resumption. Two groups of HIV structured treatment interruption patients were selected on the basis of plasma viral HIV-RNA value (> 30 000 copies/ml, branched DNA): group A (n = 14), patients with a rapid viral rebound (within one month) and group B (n = 6), patients with a delayed or no viral rebound (after a minimum of 4 months). Methods: A clinical and immunological follow-up was performed at HAART suspension (t 0), one month from suspension (t 1), at HAART resumption (t 2), and 30 days from resumption (t 3). Results: A sustained viral rebound was observed in group A patients, showing a rapid expansion of circulating CD8 T lymphocytes. In this group, the frequencies of CD8 T cells releasing IFN-γ after mitogen-induced or Gag-specific stimulation were highly increased after HAART discontinuation. Nevertheless, these CD8 T lymphocytes were mainly composed of pre-terminally differentiated cytotoxic T lymphocytes (CTL) expressing a CCR7− CD27+/− CD45RA− phenotype and a reduced amount of perforin. In contrast, group B patients showed no significant changes in immunological parameters during a prolonged drug-free period. Conclusion: These data indicate that monitoring CD8 T cell dynamics during structured treatment interruption could be clinically relevant, and new therapeutic strategies should aim qualitatively to restore CTL effector functions.

CTL*

Discontinuation

10.1097/00002030-200212060-00008

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Citations (30)

Possible protective and pathogenic roles of gamma delta T lymphocytes in HIV-infections (Review).

International Journal of Molecular Medicine (1998)

Fabrizio Poccia Matthew Wallace V Colizzi Miroslav Malkovský

alpha beta and gamma delta T lymphocytes are largely responsible for the specificity of coordinated immune responses that are of crucial importance for protection from exogenous invaders and elimination of endogenous aberrations. One of the prominent distinguishing characteristics of primate gamma delta T lymphocytes is that most of their T cell receptors for antigen (gamma delta TCRs) are, unlike alpha beta TCRs, capable of recognizing nonpeptidic antigens in an MHC-unrestricted manner. Another interesting feature is that the gamma delta T cell subpopulation displays profound qualitative and quantitative changes in individuals with various infectious diseases. For example, the most frequent human peripheral blood gamma delta T cell subset expressing Vgamma9Vdelta2 TCRs is functionally disabled and numerically decreased in some HIV-infected persons. The nonresponsiveness of Vgamma9Vdelta2 T cells is accompanied by their decreased IFNç and TNFá production. The overall level of gamma T cell activation at different stages of HIV-infection may be clinically relevant. At an initial stage of HIV-infection, the extremely potent antiviral cytotoxic activities of Vgamma9Vdelta2 T cells may limit the viral spread. At later stages of disease, Vgamma9Vdelta2 T cell dysfunction may contribute to the loss of resistance to opportunistic pathogens (such as atypical mycobacteria) and neoplasms (e.g., lymphomas) frequently associated with HIV-infections. Also, it is possible that chronic stimulation of gamma delta T cells may result in immunopathology. In particular, the massive immunologic activation that appears to be the major contributing element of AIDS immunopathogenesis could be, at least in part, driven by gamma delta T cells overstimulated by repetitive exposures to HIV.

10.3892/ijmm.1.2.409

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Citations (14)

Innate T cell immunity to HIV-infection

Vaccine (2002)

Marie‐Lise Gougeon Miroslav Malkovský Rita Casetti Chiara Agrati Fabrizio Poccia

10.1016/s0264-410x(02)00070-1

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Citations (21)

Cross-subtype Immunity against Avian Influenza in Persons Recently Vaccinated for Influenza

Emerging infectious diseases (2008)

Cristiana Gioia Concetta Castilletti Massimo Tempestilli Paola Piacentini Licia Bordi

Avian influenza virus (H5N1) can be transmitted to humans, resulting in a severe or fatal disease. The aim of this study was to evaluate the immune cross-reactivity between human and avian influenza (H5N1) strains in healthy donors vaccinated for seasonal influenza A (H1N1)/(H3N2). A small frequency of CD4 T cells specific for subtype H5N1 was detected in several persons at baseline, and seasonal vaccine administration enhanced the frequency of such reactive CD4 T cells. We also observed that seasonal vaccination is able to raise neutralizing immunity against influenza (H5N1) in a large number of donors. No correlation between influenza-specific CD4 T cells and humoral responses was observed. N1 may possibly be a target for both cellular and humoral cross-type immunity, but additional experiments are needed to clarify this point. These findings highlight the possibility of boosting cross-type cellular and humoral immunity against highly pathogenic avian influenza A virus subtype H5N1 by seasonal influenza vaccination.

Humoral immunity

Cross-reactivity

10.3201/eid1401.061283

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Citations (95)

Differentiation of Effector/Memory Vδ2 T Cells and Migratory Routes in Lymph Nodes or Inflammatory Sites

The Journal of Experimental Medicine (2003)

Francesco Dieli Fabrizio Poccia Martin Lipp Guido Sireci Nadia Caccamo

Vδ2 T lymphocytes recognize nonpeptidic antigens without presentation by MHC molecules and mount both immediate effector functions and memory responses after microbial infection. However, how Vδ2 T cells mediate different facets of a memory response remains unknown. Here, we show that the expression of CD45RA and CD27 antigens defines four subsets of human Vδ2 T cells with distinctive compartmentalization routes. Naive CD45RA+CD27+ and memory CD45RA−CD27+ cells express lymph node homing receptors, abound in lymph nodes, and lack immediate effector functions. Conversely, memory CD45RA−CD27− and terminally differentiated CD45RA+CD27− cells, which express receptors for homing to inflamed tissues, are poorly represented in the lymph nodes while abounding at sites of inflammation, and display immediate effector functions. These observations and additional in vitro experiments indicate a lineage differentiation pattern for human Vδ2 T cells that generates naive cells circulating in lymph nodes, effector/memory cells patrolling the blood, and terminally differentiated effector cells residing in inflamed tissues.

Homing (biology)

10.1084/jem.20030235

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Citations (307)

T cell selection and differentiation in AIDS disease: the model of HIV-discordant monozygotic twins.

PubMed (2007)

Chiara Agrati Cristiana Gioia Fabio Soldani Federico Martini Andrea Antinori

The model of monozygotic twins has been repeatedly studied to control the genetic and age-specific effects on HIV disease. Focusing on this natural model, the expression of CD27/CD45RA differentiation markers and the distribution of the Vbeta TCR repertoire was analyzed on CD4+ and CD8+ T cells. In our HIV-discordant monozygotic twins, a significant reduction of naive T cells and a parallel accumulation of effector/memory T cells was induced by HIV infection, as well as a skewing of T cell repertoire evidenced by VbetaTCR analysis. The block of HIV replication by highly active antiretroviral therapy (HAART) restored most of the T cell maturation and selection process, with some exception among CTL differentiation and repertoire. Altogether, the model of HIV-discordant monozygotic twins is a valuable tool showing that HAART is not able to completely restore the CTL profile.

CTL*

Monozygotic twin

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Epitope Specificity, Antibody-Dependent Cellular Cytotoxicity, and Neutralizing Activity of Antibodies to Human Immunodeficiency Virus Type 1 in Autoimmune MRL/lpr Mice

The Journal of Infectious Diseases (1993)

Valter Lombardi Roberta Placido Gabriella Scarlatti Maria Luisa Romiti Maurizio Mattei

Journal Article Epitope Specificity, Antibody-Dependent Cellular Cytotoxicity, and Neutralizing Activity of Antibodies to Human Immunodeficiency Virus Type 1 in Autoimmune MRL/lpr Mice Get access Valter Lombardi, Valter Lombardi Search for other works by this author on: Oxford Academic PubMed Google Scholar Roberta Placido, Roberta Placido Search for other works by this author on: Oxford Academic PubMed Google Scholar Gabriella Scarlatti, Gabriella Scarlatti Search for other works by this author on: Oxford Academic PubMed Google Scholar M. Luisa Romiti, M. Luisa Romiti Search for other works by this author on: Oxford Academic PubMed Google Scholar Maurizio Mattei, Maurizio Mattei Search for other works by this author on: Oxford Academic PubMed Google Scholar Francesca Mariani, Francesca Mariani Search for other works by this author on: Oxford Academic PubMed Google Scholar Fabrizio Poccia, Fabrizio Poccia Search for other works by this author on: Oxford Academic PubMed Google Scholar Paolo Rossi, Paolo Rossi Search for other works by this author on: Oxford Academic PubMed Google Scholar Vittorio Colizzi Vittorio Colizzi Reprints or correspondence: Prof. Vittorio Colizzi, Dept. of Biology, University of Rome “Tor Vergata,” Via della Ricerca Scientifica, 00133 Rome, Italy. Search for other works by this author on: Oxford Academic PubMed Google Scholar The Journal of Infectious Diseases, Volume 167, Issue 6, June 1993, Pages 1267–1273, https://doi.org/10.1093/infdis/167.6.1267 Published: 01 June 1993 Article history Received: 27 May 1992 Revision received: 22 January 1993 Published: 01 June 1993

HIV Antigens

10.1093/infdis/167.6.1267

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Citations (10)

Lymphocyte distribution and intrahepatic compartmentalization during HCV infection: a main role for MHC-unrestricted T cells.

PubMed (2002)

Chiara Agrati Carla Nisii Alessandra Oliva Gianpiero D’Offizi Carla Montesano

Liver disease

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Citations (16)