Juan F. González

Universidad Politécnica de Cartagena

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Elena de la Cuesta

Universidad Complutense de Madrid

Carmen Avendaño

Universidad Complutense de Madrid

J. Carlos Menéndez

Universidad Complutense de Madrid

Damiano Rocchi

Universidad Complutense de Madrid

John S. Gunn

The Ohio State University

Irene Ortín

Universidad San Pablo CEU

Vittorio Venturi

International Centre for Genetic Engineering and Biotechnology

Jorge Gómez-Carpintero

Universidad Complutense de Madrid

J. Domingo Sánchez

Universidad Complutense de Madrid

Brian M. M. Ahmer

The Ohio State University

Cooperative Institutions

Universidad Complutense de Madrid

The Ohio State University

Instituto de Salud Carlos III

Nationwide Children's Hospital

Centro de Investigación Biomédica en Red

Universidad de Granada

Universidad Autónoma de Madrid

Hospital Universitario 12 De Octubre

Pacific Northwest National Laboratory

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Cuerpos extraños en vías aéreas inferiores en lactantes y niños: diagnóstico y terapéutica

Anales de Pediatría (1978)

Ma de la Paz Ordoñez Guerrero F. Delgado Moreno Juan F. González

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Pictet—Spengler‐Type Reactions in 3‐Arylmethylpiperazine‐2,5‐diones. Synthesis of Pyrazinotetrahydroisoquinolines.

ChemInform (2004)

Juan F. González Elena de la Cuesta Carmen Avendaño

For Abstract see ChemInform Abstract in Full Text.

Pictet–Spengler reaction

10.1002/chin.200445218

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Machine learning analysis of serum biomarkers for cardiovascular risk assessment in chronic kidney disease

Clinical Kidney Journal (2019)

Carles Forné Serafí Cambray Marcelino Bermúdez-López Elvira Fernández Milica Božić

Abstract Background Chronic kidney disease (CKD) patients show an increased burden of atherosclerosis and high risk of cardiovascular events (CVEs). There are several biomarkers described as being associated with CVEs, but their combined effectiveness in cardiovascular risk stratification in CKD has not been tested. The objective of this work is to analyse the combined ability of 19 biomarkers associated with atheromatous disease in predicting CVEs after 4 years of follow-up in a subcohort of the NEFRONA study in individuals with different stages of CKD without previous CVEs. Methods Nineteen putative biomarkers were quantified in 1366 patients (73 CVEs) and their ability to predict CVEs was ranked by random survival forest (RSF) analysis. The factors associated with CVEs were tested in Fine and Gray (FG) regression models, with non-cardiovascular death and kidney transplant as competing events. Results RSF analysis detected several biomarkers as relevant for predicting CVEs. Inclusion of those biomarkers in an FG model showed that high levels of osteopontin, osteoprotegerin, matrix metalloproteinase-9 and vascular endothelial growth factor increased the risk for CVEs, but only marginally improved the discrimination obtained with classical clinical parameters: concordance index 0.744 (95% confidence interval 0.609–0.878) versus 0.723 (0.592–0.854), respectively. However, in individuals with diabetes treated with antihypertensives and lipid-lowering drugs, the determination of these biomarkers could help to improve cardiovascular risk estimates. Conclusions We conclude that the determination of four biomarkers in the serum of CKD patients could improve cardiovascular risk prediction in high-risk individuals.

10.1093/ckj/sfz094

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Citations (12)

SAT-407 Long-term sustained decline of anti-HCV neutralizing antibodies in HIV/HCV-coinfected patients after therapy-induced HCV clearance

Journal of Hepatology (2024)

Daniel Sepúlveda‐Crespo Camilla Volpi Rafael Amigot‐Sánchez Marta Quero‐Delgado Belén Yélamos

10.1016/s0168-8278(24)02107-x

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Nefropatía diabética:: ¿una cuestión de información?. Hipótesis del daño renal inflamatorio en la diabetes mellitus tipo 2

Nefrología (2003)

Juan F. González

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Discovery of 7-aminophenanthridin-6-one as a new scaffold for matrix metalloproteinase inhibitors with multitarget neuroprotective activity

European Journal of Medicinal Chemistry (2020)

Damiano Rocchi Cristina Blázquez-Barbadillo Mariangela Agamennone Antonio Laghezza Paolo Tortorella

10.1016/j.ejmech.2020.113061

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Sustainable Access to Acridin-9-(10H)ones with an Embedded m-Terphenyl Moiety Based on a Three-Component Reaction

Molecules (2020)

Damiano Rocchi Jorge Gómez-Carpintero Juan F. González J. Carlos Menéndez

A Ce(IV)-catalyzed three-component reaction between chalcones, anilines and β-ketoesters followed by a microwave-assisted thermal cyclization afforded 1,3-diaryl-1,2-dihydroacridin-9(10H)-ones. Their microwave irradiation in nitrobenzene, acting both as solvent and oxidant, afforded fully unsaturated 1,3-diarylacridin-9(10H)-ones, which combine acridin-9-(10H)one and m-terphenyl moieties. Overall, the route generates three C-C and one C-N bond and has the advantage of requiring a single chromatographic separation.

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Terphenyl

Nitrobenzene

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10.3390/molecules25235565

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Thermodynamic Analysis of the Activation of Glycogen Phosphorylase b Over a Range of Temperatures

Journal of Biological Chemistry (1989)

Carmen Barón Juan F. González Pedro L. Mateo Miguel Cortijo

Abstract Equilibrium dialysis and isothermal microcalorimetry experiments have been carried out to characterize the thermodynamics of the binding of AMP to glycogen phosphorylase b (EC 2.4.1.1) at pH 6.9 over the temperature range of 25-35 degrees C. Thermal titrations were performed at each temperature in various buffer systems, which have afforded the calculation of the number of protons exchanged when the AMP binds to each site in the protein. Thermodynamic parameters were obtained for the binding of AMP to the two nucleotide and the two inhibitor sites of the dimeric enzyme. The former show positive cooperativity while the latter behave as independent binding sites. A positive delta Cp value was obtained for the AMP binding to the two N sites (1.3 and 1.4 kJ K-1 mol-1), while the delta Cp was negative for the binding to the I sites (-1.9 kJ K-1 mol-1). The application of Sturtevant's method to our data (Sturtevant, J. M. (1977) Proc. Natl. Acad. Sci. U. S. A. 74, 2236-2240) and their comparison with a similar analysis undertaken with phosphorylase a (Mateo, P. L., Gonzalez, J. F., Baron, C., Lopez-Mayorga, O., and Cortijo, M. (1986) J. Biol. Chem. 261, 17067-17072) has opened the way to some understanding of the thermodynamics of the allosteric transition in the protein.

Phosphorylase kinase

10.1016/s0021-9258(18)51568-4

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Citations (23)

Privileged scaffolds in synthesis: 2,5-piperazinediones as templates for the preparation of structurally diverse heterocycles

Chemical Society Reviews (2012)

Juan F. González Irene Ortín Elena de la Cuesta J. Carlos Menéndez

2,5-Piperazinediones (2,5-diketopiperazines, DKPs) can be viewed as privileged building blocks for the synthesis of heterocyclic systems. This tutorial review aims at underscoring the large number and structural variety of nitrogen heterocycles that are available by suitable manipulation of DKP starting materials, including many bioactive compounds and natural products.

Template

Diketopiperazines

10.1039/c2cs35158g

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Citations (61)

Salmonella Biofilms Tolerate Hydrogen Peroxide by a Combination of Extracellular Polymeric Substance Barrier Function and Catalase Enzymes

Frontiers in Cellular and Infection Microbiology (2021)

Mark M. Hahn Juan F. González John S. Gunn

The ability of Salmonella enterica subspecies enterica serovar Typhi (S. Typhi) to cause chronic gallbladder infections is dependent on biofilm growth on cholesterol gallstones. Non-typhoidal Salmonella (e.g. S. Typhimurium) also utilize the biofilm state to persist in the host and the environment. How the pathogen maintains recalcitrance to the host response, and oxidative stress in particular, during chronic infection is poorly understood. Previous experiments demonstrated that S. Typhi and S. Typhimurium biofilms are tolerant to hydrogen peroxide (H2O2), but that mutations in the biofilm extracellular polymeric substances (EPSs) O antigen capsule, colanic acid, or Vi antigen reduce tolerance. Here, biofilm-mediated tolerance to oxidative stress was investigated using a combination of EPS and catalase mutants, as catalases are important detoxifiers of H2O2. Using co-cultured biofilms of wild-type (WT) bacteria with EPS mutants, it was demonstrated that colanic acid in S. Typhimurium and Vi antigen in S. Typhi have a community function and protect all biofilm-resident bacteria rather than to only protect the individual cells producing the EPSs. However, the H2O2 tolerance deficiency of a O antigen capsule mutant was unable to be compensated for by co-culture with WT bacteria. For curli fimbriae, both WT and mutant strains are tolerant to H2O2 though unexpectedly, co-cultured WT/mutant biofilms challenged with H2O2 resulted in sensitization of both strains, suggesting a more nuanced oxidative resistance alteration in these co-cultures. Three catalase mutant (katE, katG and a putative catalase) biofilms were also examined, demonstrating significant reductions in biofilm H2O2 tolerance for the katE and katG mutants. Biofilm co-culture experiments demonstrated that catalases exhibit a community function. We further hypothesized that biofilms are tolerant to H2O2 because the physical barrier formed by EPSs slows penetration of H2O2 into the biofilm to a rate that can be mitigated by intra-biofilm catalases. Compared to WT, EPS-deficient biofilms have a heighted response even to low-dose (2.5 mM) H2O2 challenge, confirming that resident bacteria of EPS-deficient biofilms are under greater stress and have limited protection from H2O2. Thus, these data provide an explanation for how Salmonella achieves tolerance to H2O2 by a combination of an EPS-mediated barrier and enzymatic detoxification.

Extracellular polymeric substance

10.3389/fcimb.2021.683081

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Citations (27)