Jan Sawicki

Medical University of Lublin

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Ireneusz Sowa

Medical University of Lublin

Magdalena Wójciak

Medical University of Lublin

Sławomir Dresler

Maria Curie-Skłodowska University

Maciej Strzemski

Medical University of Lublin

Michał Staniak

Medical University of Lublin

M Latalski

Medical University of Lublin

Urszula Doboszewska

Jagiellonian University

Piotr Właź

Maria Curie-Skłodowska University

Katarzyna Socała

Maria Curie-Skłodowska University

Dorota Nieoczym

Maria Curie-Skłodowska University

Cooperative Institutions

Medical University of Lublin

Polish Academy of Sciences

Maria Curie-Skłodowska University

Jagiellonian University

Warsaw University of Technology

University of Trnava

University of Life Sciences in Poznań

Maj Institute of Pharmacology

Instytut Medycyny Wsi im. Witolda Chodźki

Medical University of Warsaw

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Fusing individual choices into a group decision with help of software agents and semantic technologies

Konrad Ponichtera Mikołaj Małkiński Jan Sawicki Maria Ganzha Marcin Paprzycki

What happens when hunger, individual eating constraints and student economy are combined with some computer skills? This contribution introduces a agent-semantic application, which expedites process of choosing where and what to eat, when a group of hungry students would like to meet and have lunch together. Furthermore, individual dietary restrictions are taken into account. Specifically, software agents are used to facilitate negotiation mechanisms used to find (i) place to meet, and (ii) common food, while semantic technologies are used to represent food and user profiles (i.e. food allergies). The initial system has been implemented and validated on the basis of selected use case scenarios.

Software agent

10.1109/icaccaf.2018.8776739

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Citations (1)

2-Chloro-2? deoxyadenosine prevents angiopathic changes in cerebral arteries in experimental SAH in rabbits

Acta Neurochirurgica (1993)

M Ryba Paweł Grieb M. Walski Jan Sawicki M Pastuszko

Neuroradiology

Deoxyadenosine

Interventional radiology

10.1007/bf01446997

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Citations (4)

TC-G 1008 facilitates epileptogenesis by acting selectively at the GPR39 receptor but non-selectively activates CREB in the hippocampus of pentylenetetrazole-kindled mice

Cellular and Molecular Life Sciences (2023)

Urszula Doboszewska Katarzyna Socała Mateusz Pieróg Dorota Nieoczym Jan Sawicki

The pharmacological activation of the GPR39 receptor has been proposed as a novel strategy for treating seizures; however, this hypothesis has not been verified experimentally. TC-G 1008 is a small molecule agonist increasingly used to study GPR39 receptor function but has not been validated using gene knockout. Our aim was to assess whether TC-G 1008 produces anti-seizure/anti-epileptogenic effects in vivo and whether the effects are mediated by GPR39. To obtain this goal we utilized various animal models of seizures/epileptogenesis and GPR39 knockout mice model. Generally, TC-G 1008 exacerbated behavioral seizures. Furthermore, it increased the mean duration of local field potential recordings in response to pentylenetetrazole (PTZ) in zebrafish larvae. It facilitated the development of epileptogenesis in the PTZ-induced kindling model of epilepsy in mice. We demonstrated that TC-G 1008 aggravated PTZ-epileptogenesis by selectively acting at GPR39. However, a concomitant analysis of the downstream effects on the cyclic-AMP-response element binding protein in the hippocampus of GPR39 knockout mice suggested that the molecule also acts via other targets. Our data argue against GPR39 activation being a viable therapeutic strategy for treating epilepsy and suggest investigating whether TC-G 1008 is a selective agonist of the GPR39 receptor.

Knockout mouse

Kindling model

Gene knockout

10.1007/s00018-023-04766-z

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Citations (7)

Text embeddings and clustering for characterizing online communities on Reddit

Zenodo (CERN European Organization for Nuclear Research) (2023)

Jan Sawicki

10.5281/zenodo.8150193

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Citations (0)

2-Chloro-2'-deoxyadenosine (2-CdA) combined with cyclosporine A successfully prevents rejection of fetal brain stem allograft in rabbits.

PubMed (1994)

Paweł Grieb M Ryba Wiktor A. Janczewski Jan Sawicki J Jagielski

Immunosuppression

Synaptogenesis

Source

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Citations (2)

Survival and maturation of heterotopic fetal brain stem xenografts after treatment with 2-chlorodeoxyadenosine and cyclosporine A

Acta Neurobiologiae Experimentalis (1995)

M Ryba Paweł Grieb Wiktor A. Janczewski Jan Sawicki J Jagielski

Brain stem halves from fetal rabbits were transplanted to the caudate nucleus area of adult rats. The animals were treated postoperatively with cyclosporine A (CsA) and 2-chlorodeoxyadenosine (CdA) for three days, and with CdA alone for the next 13 days. The treatment started at the day of implantation, and in some animals it was repeated starting at day 36 after grafting (at the time when signs of a light inflammatory reaction appeared in some grafts). Grafts survived and matured histologically, and no signs of acute rejection were observed up to the 90th day. In some grafts we recorded phasic neuronal activities similar to the respiratory-related neural activities characteristic for the adult brain stem. Immunosuppressive with CdA and CsA deserves further evaluation in fetal brain grafting.

Caudate nucleus

10.55782/ane-1995-1085

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Citations (3)

Metabolic responses of Carlina acaulis L. to chronic and acute cadmium stress: insights into chelation mechanisms, non-enzymatic antioxidants, and specialized metabolism

Research Square (Research Square) (2020)

Sławomir Dresler Maciej Strzemski Jozef Kováčik Jan Sawicki Michał Staniak

Abstract The authors have withdrawn this preprint from Research Square

Preprint

Metabolic pathway

10.21203/rs.2.20457/v2

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Citations (0)

Optimization of high-resolution continuum source graphite furnace atomic absorption spectrometry for direct analysis of selected trace elements in whole blood samples

Talanta (2016)

Magdalena Wójciak Wojciech Szwerc Maciej Strzemski Zoltan Wichłacz Jan Sawicki

Matrix (chemical analysis)

Certified reference materials

Repeatability

Sample Preparation

10.1016/j.talanta.2016.12.077

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Citations (16)

The Effects and Mechanisms of Action of TC-G 1008, GPR39 Agonist, in Animal Models of Seizures and Epilepsy

Research Square (Research Square) (2022)

Urszula Doboszewska Katarzyna Socała Mateusz Pieróg Dorota Nieoczym Jan Sawicki

Abstract Background and purpose: The G-protein coupled receptor 39 (GPR39) may be activated by zinc ions. Activation of GPR39 was suggested as a novel pharmacological strategy for treating seizures. Experimental approach: We utilized a specific agonist of GPR39, TC-G 1008, and the nonspecific agonist, zinc chloride and a variety of models of acute seizures or a chronic model of epilepsy which were induced in non-genetically modified mice, GPR39 knockout mice or in zebrafish larvae. We examined total serum zinc (by Inductively Coupled Plasma Optical Emission Spectrometry) as well as intracellular zinc ([Zn 2+ ]I) (by Zinpyr-1 staining) concentrations and the expression of selected proteins (by Western blot) which are associated with GPR39 signaling in the hippocampus. Key results: Liquid chromatography tandem mass spectrometry analysis showed that TC-G 1008 is brain penetrant. TC-G 1008 decreased the seizure threshold in the maximal electroshock seizure (MES) threshold test, but it increased the seizure threshold in the 6-Hz induced seizure threshold test. The behavioral effects of TC-G 1008 and MES or 6-Hz seizure were accompanied by alterations in hippocampal [Zn 2+ ]I. TC-G 1008 increased the mean duration of EEG discharges in response to pentylenetetrazole (PTZ) in zebrafish larvae and facilitated the development of PTZ kindling in mice. Using GPR39 knockout mouse line, generated by the CRISPR-Cas-9 method, we showed that GPR39 is a target for TC-G 1008 regarding PTZ-induced epileptogenesis. Conclusion and implications: Our in vivo data obtained using TC-G 1008 generally argue against GPR39 activation as a therapeutic strategy for alleviating seizures/epilepsy.

Convulsion

Knockout mouse

Seizure threshold

10.21203/rs.3.rs-1182423/v1

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Citations (1)

Some Natural Photosensitizers and Their Medicinal Properties for Use in Photodynamic Therapy

Molecules (2022)

Tomasz Kubrak Przemysław Kołodziej Jan Sawicki Artur Mazur Katarzyna Koziorowska

Despite significant advances in early diagnosis and treatment, cancer is one of the leading causes of death. Photodynamic therapy (PDT) is a therapy for the treatment of many diseases, including cancer. This therapy uses a combination of a photosensitizer (PS), light irradiation of appropriate length and molecular oxygen. The photodynamic effect kills cancer cells through apoptosis, necrosis, or autophagy of tumor cells. PDT is a promising approach for eliminating various cancers but is not yet as widely applied in therapy as conventional chemotherapy. Currently, natural compounds with photosensitizing properties are being discovered and identified. A reduced toxicity to healthy tissues and a lower incidence of side effects inspires scientists to seek natural PS for PDT. In this review, several groups of compounds with photoactive properties are presented. The use of natural products has been shown to be a fruitful approach in the discovery of novel pharmaceuticals. This review focused on the anticancer activity of furanocoumarins, polyacetylenes, thiophenes, tolyporphins, curcumins, alkaloid and anthraquinones in relation to the light-absorbing properties. Attention will be paid to their phototoxic and anti-cancer effects on various types of cancer.

Phototoxicity

Cancer Therapy

10.3390/molecules27041192

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Citations (80)