İA

İrfan Aydın

Fenerbahçe University

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i-10 index

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Hatice Yorulmaz

Haliç University

EÖ

Elif Özkök

Istanbul University

Abdullah Algın

Sağlık Bilimleri Üniversitesi

Gulten Ates

Istanbul Yeni Yüzyıl University

Yaşar Altun

Adıyaman University

Mıne Ergüven

Istanbul Aydın University

Fatih Selvi

Sağlık Bilimleri Üniversitesi

ÖY

Özlem YILDIRIM

Altınbaş University

Handan Tanyıldızı Kökkülünk

Altınbaş University

MÖ

Mehmet Özgür Erdoğan

Bakırköy Dr.Sadi Konuk Eğitim ve Araştırma Hastanesi

Cooperative Institutions

Istanbul University

Istanbul University-Cerrahpaşa

Sağlık Bilimleri Üniversitesi

University of Health Science

University of Health Sciences

Haydarpaşa Numune Eğitim ve Araştırma Hastanesi

Ministry of Health

Bakırköy Psychiatric Hospital

Istanbul Yeni Yüzyıl University

Adıyaman University

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Ecbalium Elaterium Kullanımına Bağlı Gelişen Uvular Ödem: Olgu Sunumu

DergiPark (Istanbul University) (2012)

İrfan Aydın Sevdegül KARADAŞ Hayriye Gönüllü Fatih Selvi

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The impact of pretreatment with simvastatin on kidney tissue of rats with acute sepsis

Physiology International (2017)

Elif Özkök Hatice Yorulmaz Gulten Ates İrfan Aydın Mıne Ergüven

It has been reported that changes in cytokine levels affect mitochondrial functions, levels of hypoxia-inducible factor α (HIF-1α), and tissue damage during sepsis. We aimed to investigate the effects of simvastatin pretreatment on mitochondrial enzyme activities, and on levels of ghrelin, HIF-1α, and thiobarbituric acid reactive substances (TBARS) in kidney tissue during sepsis. Rats were separated into four groups, namely, control, lipopolysaccharides (LPS) (20 mg/kg), simvastatin (20 mg/kg), and simvastatin + LPS. We measured the levels of mitochondrial enzyme activities and TBARS in the kidney using spectrophotometry. The histological structure of the kidney sections was examined after staining with hematoxylin and eosin. Tumor necrosis factor α (TNF-α), IL-10, HIF-1α, and ghrelin immunoreactivity were examined using proper antibodies. In tissue, TNF-α ( p < 0.01) and HIF-1α ( p < 0.05) levels were increased in the simvastatin + LPS and LPS groups. TBARS levels were higher in the LPS group than in the other groups ( p < 0.01), but they were similar in the simvastatin + LPS and control groups ( p > 0.05). Ghrelin immunoreactivity was lower in the LPS group ( p < 0.05) and higher in the simvastatin + LPS group than in the LPS group ( p < 0.01). We observed tubular damage in the sections of the LPS group. There were no differences in mitochondrial enzyme activities between the groups ( p > 0.05). We observed that pretreatment of simvastatin caused favorable changes on ghrelin and TBARS levels in rats with sepsis.

TBARS

Thiobarbituric acid

10.1556/2060.104.2017.2.8

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Effects of Ghrelin on Inflammation and Oxidative Stress Parameters in Sepsis-induced Liver Tissue of Rat

Asian Journal of Animal and Veterinary Advances (2016)

Gulten Ates Elif Özkök Hatice Yorulmaz İrfan Aydın Şule Tamer

Background: Sepsis is a life-threatening systemic inflammatory syndrome, that affects many organs or systems.Ghrelin is released in many tissues and organs and it has anti-inflammatory and antioxidant effects.Objective: In this study, the effects of exogenous ghrelin on Tumor Necrosis Factor-α (TNF-α), interleukin-10 (IL-10), superoxide dismutase (SOD), thiobarbituric acid reactive substances (TBARS) and ghrelin levels were aimed to investigate in liver tissue after inducing sepsis with lipopolysaccharide (LPS).Materials and Methods: Wistar albino rats were divided into four groups, control, LPS, ghrelin, ghrelin+LPS.The LPS group was established by giving LPS twice (5 mg kgG 1 , i.v. and i.p., respectively).Ghrelin (10 nmol kgG 1 ) was given intravenously.Tissue levels of TNF-α, IL-10, ghrelin and the activities of SOD were mesured with using an ELISA kit.The levels of TBARS were determined spectrophotometrically.Results: The levels of TNF-α and IL-10 were found higher in the LPS group than controls (p<0.01 and p<0.05, respectively) and TBARS levels were increased in the LPS group compared with the controls, ghrelin and ghrelin+LPS groups (p<0.01,p<0.05 and p<0.01, respectively).The SOD and ghrelin levels were higher in the LPS group than in the others, ghrelin levels were also increased in the ghrelin group compared with the controls (p<0.05).According to histologic imaging, there were enlargements in sinusoids in the LPS group.In the ghrelin+LPS group, the same image was seen as in the controls.Conclusion: Ghrelin treatment attenuated anti-and pro-inflammatory cytokines, TBARS levels and SOD activities and also reduced liver tissue damage in a time and dose dependent manner during sepsis.

Liver tissue

10.3923/ajava.2017.17.23

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Determination of Radiation Exposure of Students During Their Internships Using OSL Dosimeter

DergiPark (Istanbul University) (2022)

Handan Tanyıldızı Kökkülünk İrfan Aydın Özlem YILDIRIM

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Early phases of sepsis: effects of simvastatin on mitochondrial enzyme activities in kidney tissue in rats

Critical Care (2014)

Elif Özkök Hatice Yorulmaz Gulten Ates İrfan Aydın A.T. Tamer

Introduction: During the course of systemic inflammation, most of the immune cell types get activated to a certain degree as part of, or contributing to, the cascade of physiopathological events.Whether for some cells, classically phagocytes of the innate immune system, it is clear that direct sensing of pathogen-associated molecular patterns leads to activation initiating systemic inflammation, the picture is not so clear for natural killer (NK) cells.While NK cells have been shown to express toll-like receptors (TLR), the role of these receptors on NKs during systemic inflammation has not been directly addressed.Methods: To directly assess the role of TLR expression on NK cells we used an adoptive transfer model in which NKs purified from the spleens of WT, TLR4KO and TLR2/4DKO mice were transferred intravenously to RAG2 -/-γc -/-(devoid of T, B and NK cells).Five days after reconstitution the mice were challenged intraperitoneally with conventional or TLR-grade lipopolysaccharide (LPS).Immune cell activation and production of IFNγ by NK cells was determined after 6 hours by FACS analysis.Results: We observed no differences in reconstitution of the recipient mice with NK cells from different backgrounds suggesting no difference in trafficking and survival of the transferred cells.At 6 hours after LPS challenge, WT, TLR4KO or TLR2/4DKO NK cells recovered from the spleen and lungs of RAG2 -/-γc -/-mice showed comparable levels of CD69 activation marker expression.Intracellular labeling for IFNγ in NK cells also revealed no significant differences.Conclusion: Whether there is a role for direct TLR signaling on NK cells remains the objective of further investigations; however, our data show that in the course of a systemic inflammatory process, like endotoxinemia, the expression of TLR2 and TLR4 by NK cells makes no difference in terms of their activation and secretion of IFNγ P2 Role of 6-hour, 12-hour, and 24-hour lactate clearance in mortality of severe sepsis and septic shock patients.

10.1186/cc14060

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Stroke Patients with Sinus Rhythm and Atrial Fibrillation: Comparison of Echocardiography Findings

Eurasian Journal of Emergency Medicine (2019)

Abdullah Algın Mehmet Özgür Erdoğan Şahin Çolak İrfan Aydın İsmail Tayfur

Materials and MethodsStroke patients admitted to the emergency department of Adıyaman University Hospital between 2014 and 2016 were analyzed retrospectively.Stroke patients were identified with the ICD code I1.68 and AF patients were identified with the ICD code I48.All subsequent stroke patients' data were recorded to standard Abstract Aim: To compare the echocardiographic findings in stroke patients with sinus rhythm and atrial fibrillation (AF).Materials and Methods: Descriptive, cross-sectional study.Place and Duration of Study:

Normal Sinus Rhythm

Stroke

10.4274/eajem.galenos.2017.18199

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Investigation of the potential effects of alpha-lipoic acid on human degenerated intervertebral disc tissue primary cell cultures

Turkish Neurosurgery (2021)

İrfan Aydın Duygu Yaşar Şirin Numan Karaarslan Hanefi Özbek

To investigate the supplementation of alpha-lipoic acid (ALA) at the molecular level to determine its effect on primary cell cultures prepared from human intervertebral disc (IVD) tissue in an in vitro environment.Human primary cell cultures were prepared from IVD tissue resected during surgery. While cell cultures without ALA supplementation formed the control group, those with ALA supplementation formed the study group. All cell groups were stained using acridine orange/propidium iodide (AO/PI), and the incidence of apoptotic cell death was determined under a fluorescent microscope. Cell surface morphology and extracellular matrix (ECM) structures were evaluated under an invert light microscope. Simultaneously, cell proliferation was evaluated by MTT?ELISA analysis, and the expressions of chondroadherin (CHAD), cartilage oligomeric protein (COMP), interleukin-1 beta (IL-1?), and matrix metalloproteinase (MMP)-7 and-19, which are genes associated with ECM regulation, were tested using qRT?PCR. The data obtained were evaluated statistically using Tukey?s honestly significant difference (HSD) test after analysis of variance (ANOVA) was performed. The alpha significance value was accepted as < .05.Compared to the cells in the control group, it was observed that both proliferation was suppressed and ECM structures deteriorated in the cells in the study group.Also, it was reported that the all-gene expression levels changed. ALA supplementation can negatively affect human IVD primary cell cultures in an in vitro environment.

Acridine orange

Propidium iodide

MTT assay

Intervertebral Disc

10.5137/1019-5149.jtn.34917-21.3

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Citations (2)

Adıyaman İlinde İnme Tiplerinin Demografik Özellikleri

Türk Nöroloji Dergisi (2018)

Yaşar Altun İrfan Aydın Abdullah Algın

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Effect of simvastatin on mitochondrial enzyme activities, ghrelin, hypoxia-inducible factor 1α in hepatic tissue during early phase of sepsis.

PubMed (2015)

Hatice Yorulmaz Elif Özkök Mıne Ergüven Gulten Ates İrfan Aydın

We aimed to investigate the effects of prior treatment of simvastatin on mitochondrial enzyme, ghrelin, and hypoxia-inducible factor 1 α (HIF-1 α) on hepatic tissue in rats treated with Lipopolysaccharides (LPS) during the early phase of sepsis. Rats were divided into four groups: control, LPS (20 mg/kg, i.p.), Simvastatin (20 mg/kg, p.o.), and LPS + Simvastatin group. We measured citrate synthase, complex I, II, I-III, II-III enzymes activities, serum and tissue levels of TNF-α, IL-10 using ELISA. Liver sections underwent histopathologic examination and TNF-α, IL-10, HIF-1α and ghrelin immunoreactivity were examined using immunohistochemistry methods. There were no differences in all groups for mitochondrial enzyme activities. In terms of both ELISA and immunohistochemistry findings; the levels of serum and tissue TNF-α and IL-10 were higher in the experimental groups than controls (P < 0.05). In the LPS group, the hepatocyte cell membrane and sinusoid structure were damaged. In the Simvastatin +LPS group, hepatocytes and sinusoidal cord structure were partially improved. For HIF-1α, in all experimental groups immunoreactivity was increased (P < 0.05). In the Simvastatin group, Ghrelin levels were increased in comparison with the other groups (P < 0.01). Ghrelin levels were greatly decreased in LPS (P < 0.05). We observed that the degree of hepatocellular degeneration was partially reduced depending on the dosage and duration of prior simvastatin treatment with LPS, probably due to alterations of Ghrelin and HIF-1α levels.

Hypoxia

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Demographic Characteristics of Stroke Types in Adıyaman

Turkish Journal Of Neurology (2018)

Yaşar Altun İrfan Aydın Abdullah Algın

Objective:We aimed to investigate the etiologic, demographic, clinical features, and risk factors of patients who were hospitalized, followed up, and treated due to stroke in our hospital.Materials and Methods: In this study, the hospital records of patients diagnosed as having stroke or cerebrovascular disease (CVD) between January 2013 and January 2015 in Adıyaman University Training and Research Hospital, where patients with stroke are frequently admitted or transferred, were retrospectively investigated.Results: In the past two years, 683 patients were followed-up or treated for stroke and CVD in our hospital.Stroke patients constituted 0.06% of all emergency admissions.Of these patients, 87.8% were diagnosed as having ischemic stroke and 8.6% were diagnosed as having hemorrhagic stroke.The sex distribution was almost equal (50.8% were males and 49.2% were females).Based on the results of brain scans performed during admission, radiologically, 498 (81.1%) patients showed evidence of anterior system involvement and 116 (18.9%) patients showed evidence of posterior system involvement.No focal areas were observed in computed tomography scans in 508 patients.However, diffusion magnetic resonance findings of these 508 patients were pathologic.Most of the patients with stroke were aged above 65 years (74.9%).The most common symptom during admission was unilateral muscle weakness (63.3%), and 58.6% of the patients were brought to the hospital by ambulance. Conclusion:As well as the results of our study being parallel to previous studies, our study also reflects the majority of stroke data in Adıyaman, Turkey, and provides significant results regarding our geographic region.

Stroke

10.4274/tnd.94103

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Citations (4)