M. Carmen Cañizares

Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora"

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María D. García‐Pedrajas

Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora"

Encarnación Pérez-Artés

Instituto de Agricultura Sostenible

Enrique Moriones

Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora"

Vicente Pallás

Universitat Politècnica de València

Jesús Navas‐Castillo

Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora"

George P. Lomonossoff

Norwich Research Park

C.G. Lemus-Minor

Instituto de Agricultura Sostenible

Yazmín Landeo-Ríos

Charles University

Khalid Amari

University Mohamed El Bachir El Ibrahimi of Bordj Bou Arreridj

Almudena Torres-Trenas

Consejo Superior de Investigaciones Científicas

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Consejo Superior de Investigaciones Científicas

Universidad de Málaga

Instituto de Investigación Biomédica de Málaga

Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora"

Hospital Regional Universitario de Málaga

Hospital Universitario Puerta de Hierro Majadahonda

John Innes Centre

Universidad Autónoma de Madrid

Norwich Research Park

Instituto de Agricultura Sostenible

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A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioides

Viruses (2020)

Andrés Gustavo Jacquat Martín G. Theumer M. Carmen Cañizares Humberto Debat Juliana Iglesias

Mycoviruses appear to be widespread in Fusarium species worldwide. The aim of this work was to identify mycoviral infections in Fusarium spp., isolated from maize and sorghum grown in Argentina, and to estimate their potential effects on the pathogenicity and toxigenesis of the host fungus towards maize. Mycoviruses were identified in 2 out of 105 isolates analyzed; Fusarium verticillioides strain Sec505 and Fusarium andiyazi strain 162. They were characterized as members of the genus Mitovirus by high-throughput sequencing and sequence analysis. The F. verticillioides mitovirus was a novel mycovirus whereas the F. andiyazi mitovirus was found to be a new strain of a previously identified mitovirus. We have named these mitoviruses, Fusarium verticillioides mitovirus 1 (FvMV1) and Fusarium andiyazi mitovirus 1 strain 162 (FaMV1-162). To our knowledge, FvMV1 is the first mycovirus reported as naturally infecting F. verticillioides, the major causal agent of ear rot and fumonisin producer in corn. Both mitoviruses exhibited 100% vertical transmission rate to microconidia. The Fa162 strain infected with FaMV1-162 did not show phenotypic alterations. In contract, F. verticillioides Sec505 infected with FvMV1 showed increased virulence as well as microconidia and fumonisin-B1 production, compared with two uninfected strains. These results suggest that FvMV1 could have a role in modulating F. verticillioides pathogenicity and toxin production worth further exploring.

Mycovirus

Fumonisin

Strain (injury)

10.3390/v12101161

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Citations (16)

The Fusarium Circinatum Gene Fcrho1, Encoding a Putative Rho1 GTPase, Is Involved in Vegetative Growth but Dispensable for Pathogenic Development

Forests (2018)

E. Jordán Muñoz‐Adalia M. Carmen Cañizares Mercedes Fernández Julio Javier Díez María D. García‐Pedrajas

Fusarium circinatum is the causal agent of pine pitch canker (PPC), one of the most devastating forest diseases worldwide. This fungus causes severe damping-off in pine seedlings and growth reduction, wilting and the development of cankers in pine forests and plantations. A draft of the complete genome sequence of this phytopathogen was recently made available. This information was used to annotate in silico the gene Fcrho1 as a putative homolog of Rho1 GTPase genes. In this study, we generated Fcrho1 deletion mutants in two F. circinatum wildtype strains isolated from damaged trees in northern Spain. For that, we used a modified version of the OSCAR methodology, an approach not previously used in F. circinatum that allows the generation of deletion constructs in a single cloning step. The conidiation and spore germination of the resulting deletion mutants were not affected, neither the hyphal morphology. However, the mutant strains showed significantly reduced growth in vitro and more foamy macroscopic hyphal morphology than their corresponding ectopic and wildtype strains. Finally, an in vivo virulence assay showed that the reduced in vitro growth rate characteristic to the deletion mutants does not impact their pathogenicity.

Canker

Wilting

Conidiation

Wild type

10.3390/f9110684

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Citations (5)

Molecular Mechanisms Controlling the Disease Cycle in the Vascular Pathogen Verticillium dahliae Characterized Through Forward Genetics and Transcriptomics

Molecular Plant-Microbe Interactions (2020)

Jorge Luis Sarmiento-Villamil Nicolás García‐Pedrajas M. Carmen Cañizares María D. García‐Pedrajas

The soil-borne pathogen Verticillium dahliae has a worldwide distribution and a plethora of hosts of agronomic value. Molecular analysis of virulence processes can identify targets for disease control. In this work, we compared the global gene transcription profile of random T-DNA insertion mutant strain D-10-8F, which exhibits reduced virulence and alterations in microsclerotium formation and polar growth, with that of the wild-type strain. Three genes identified as differentially expressed were selected for functional characterization. To produce deletion mutants, we developed an updated version of one-step construction of Agrobacterium-recombination-ready plasmids (OSCAR) that included the negative selection marker HSVtk (herpes simplex virus thymidine kinase gene) to prevent ectopic integration of the deletion constructs. Deletion of VdRGS1 (VDAG_00683), encoding a regulator of G protein signaling (RGS) protein and highly upregulated in the wild type versus D-10-8F, resulted in phenotypic alterations in development and virulence that were indistinguishable from those of the random T-DNA insertion mutant. In contrast, deletion of the other two genes selected, vrg1 (VDAG_07039) and vvs1 (VDAG_01858), showed that they do not play major roles in morphogenesis or virulence in V. dahliae. Taken together the results presented here on the transcriptomic analysis and phenotypic characterization of D-10-8F and ∆VdRGS1 strains provide evidence that variations in G protein signaling control the progression of the disease cycle in V. dahliae. We propose that G protein-mediated signals induce the expression of multiple virulence factors during biotrophic growth, whereas massive production of microsclerotia at late stages of infection requires repression of G protein signaling via upregulation of VdRGS1 activity.

Verticillium dahliae

Wild type

10.1094/mpmi-08-19-0228-r

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Citations (7)

Characterization of an unclassified Mycovirus from Verticillium Dahliae and analysis of its transmision between defoliating and non-defoliating isolates

M. Carmen Cañizares E. Ostos Francisco Javier López‐Escudero Encarnación Pérez-Artés María D. García‐Pedrajas

Poster presentado en el XVIII Congreso de la Sociedad Espanola de Fitopatologia (SEF), celebrado en Palencia del 20 al 23 de septiembre de 2016.

Mycovirus

Characterization

Verticillium dahliae

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First Report on «Hop Stunt Viroid» (HSVd) from Some Mediterranean Countries

DOAJ (DOAJ: Directory of Open Access Journals) (2007)

A. Myrta B. Di Terlizzi C. Varveri I. Gavriel Khalid Amari

10.14601/phytopathol_mediterr-1569

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[The leafspot of apricot tree: studies on its aetiology and its incidence in the Murcia Region [Spain]]

Vicente Pallás Fenollar Aparicio M. Carmen Cañizares Khalid Amari Sánchez-Pina

Tree (set theory)

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Plant tissue distribution and chemical inactivation of six carnation viruses

Crop Protection (2006)

Jesús Á. Sánchez-Navarro M. Carmen Cañizares Emilio Cano Vicente Pallás

Petal

Caryophyllaceae

Turnip mosaic virus

Sepal

10.1016/j.cropro.2006.09.016

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Citations (19)

Resistance toTomato yellow leaf curl virusAccumulation in the Tomato Wild RelativeSolanum habrochaitesAssociated with the C4 Viral Protein

Molecular Plant-Microbe Interactions (2011)

Diego M. Tomás M. Carmen Cañizares Jesús Abad R. Fernández-Muñoz Enrique Moriones

Tomato yellow leaf curl disease (TYLCD) is a severe threat to tomato crops worldwide and is caused by Tomato yellow leaf curl virus (TYLCV) and several other begomoviruses (genus Begomovirus, family Geminiviridae). Host plant resistance is the best TYLCD control method but limited sources of resistance are available. In this study, two Solanum habrochaites TYLCD-resistance sources, EELM-388 and EELM-889, were found after a wide germplasm screening and were further characterized. A consistent resistance to the widely distributed strain TYLCV-IL was observed when plants were inoculated by Bemisia tabaci or by agroinoculation using an infectious clone, with no symptoms or virus accumulation observed in inoculated plants. Moreover, the resistance was effective under field conditions with high TYLCD pressure. Two independent loci, one dominant and one recessive, were associated with EELM-889 resistance. The study shows these loci to be distinct from that of the resistance gene (Ty-1 gene) commonly deployed in commercial tomato cultivars. Therefore, both kinds of resistance could be combined to provide improved resistance to TYLCD. Four additional TYLCD-associated viruses were challenged, showing that the resistance always prevented symptom expression, although systemic infection could occur in some cases. By using chimeric and mutant expression constructs, the C4 protein was shown to be associated with the ability to result in effective systemic infection.

Germ plasm

Leaf curl

Geminiviridae

10.1094/mpmi-12-10-0291

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Citations (31)

First Report on "Hop Stunt Viroid" (HSVd) from Some Mediterranean Countries.

Phytopathologia Mediterranea (2000)

Khalid Amari M. Carmen Cañizares A. Myrta Sead Sabanadzovic M. Srhiri

Hop stunt viroid (HSVd) has a very wide host range including most stone fruit trees. Among them, apricot is one of the most important host crops in the Mediterranean basin. In this study non-isotopic molecular hybridisation revealed, for the first time, the presence of HSVd on apricot in four Mediterranean countries (Cyprus, Greece, Morocco and Turkey). The results obtained by this technique were confirmed by northern-blot and RT-PCR analyses. The data presented in this work indicate a wider geographical distribution of this viroid than hitherto known and emphasise the need for this kind of study as part of the control effort.

Mediterranean Basin

Mediterranean area

10.1400/57858

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Citations (8)

Tomato yellow leaf curl viruses: ménage à trois between the virus complex, the plant and the whitefly vector

Molecular Plant Pathology (2010)

J. A. DIAZ M. Carmen Cañizares Enrique Moriones Eduardo R. Bejarano Henryk Czosnek

Tomato yellow leaf curl disease (TYLCD) is one of the most devastating viral diseases affecting tomato crops in tropical, subtropical and temperate regions of the world. Here, we focus on the interactions through recombination between the different begomovirus species causing TYLCD, provide an overview of the interactions with the cellular genes involved in viral replication, and highlight recent progress on the relationships between these viruses and their vector, the whitefly Bemisia tabaci.The tomato yellow leaf curl virus-like viruses (TYLCVs) are a complex of begomoviruses (family Geminiviridae, genus Begomovirus) including 10 accepted species: Tomato yellow leaf curl Axarquia virus (TYLCAxV), Tomato yellow leaf curl China virus (TYLCCNV), Tomato yellow leaf curl Guangdong virus (TYLCGuV), Tomato yellow leaf curl Indonesia virus (TYLCIDV), Tomato yellow leaf curl Kanchanaburi virus (TYLVKaV), Tomato yellow leaf curl Malaga virus (TYLCMalV), Tomato yellow leaf curl Mali virus (TYLCMLV), Tomato yellow leaf curl Sardinia virus (TYLCSV), Tomato yellow leaf curl Thailand virus (TYLCTHV), Tomato yellow leaf curl Vietnam virus (TYLCVNV) and Tomato yellow leaf curl virus(TYLCV). We follow the species demarcation criteria of the International Committee on Taxonomy of Viruses (ICTV), the most important of which is an 89% nucleotide identity threshold between full-length DNA-A component nucleotide sequences for begomovirus species. Strains of a species are defined by a 93% nucleotide identity threshold.The primary host of TYLCVs is tomato (Solanum lycopersicum), but they can also naturally infect other crops [common bean (Phaseolus vulgaris), sweet pepper (Capsicum annuum), chilli pepper (C. chinense) and tobacco (Nicotiana tabacum)], a number of ornamentals [petunia (Petuniaxhybrida) and lisianthus (Eustoma grandiflora)], as well as common weeds (Solanum nigrum and Datura stramonium). TYLCVs also infect the experimental host Nicotiana benthamiana.Infected tomato plants are stunted or dwarfed, with leaflets rolled upwards and inwards; young leaves are slightly chlorotic; in recently infected plants, fruits might not be produced or, if produced, are small and unmarketable. In common bean, some TYLCVs produce the bean leaf crumple disease, with thickening, epinasty, crumpling, blade reduction and upward curling of leaves, as well as abnormal shoot proliferation and internode reduction; the very small leaves result in a bushy appearance.

Leaf curl

Geminiviridae

Whitefly

10.1111/j.1364-3703.2010.00618.x

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Citations (138)