MJ

Matthew J. Provenzano

Cincinnati Children's Hospital Medical Center

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Co-Authors

Frederick E. Domann

Radiation Oncology Associates

Matthew P. Fitzgerald

University of Nebraska Medical Center

Daniel Choo

University of Cincinnati Medical Center

J. Jason Clark

University of Iowa

Marlan R. Hansen

University of Iowa

Kristi Chang

Linkou Chang Gung Memorial Hospital

Alessandro de Alarcón

Cincinnati Children's Hospital Medical Center

John H. Greinwald

Cincinnati Children's Hospital Medical Center

Michael J. Rutter

Cincinnati Children's Hospital Medical Center

Jareen Meinzen‐Derr

Cincinnati Children's Hospital Medical Center

Cooperative Institutions

University of Iowa

University of Cincinnati

University of Cincinnati Medical Center

Cincinnati Children's Hospital Medical Center

University of Iowa Hospitals and Clinics

Harvard University

Temple University

Johns Hopkins University

American Academy of Otolaryngology — Head and Neck Surgery

Iowa City Public Library

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p75NTR and Sortilin Increase After Facial Nerve Injury

The Laryngoscope (2008)

Matthew J. Provenzano Ningyong Xu Mark R. Ver Meer J. Jason Clark Marlan R. Hansen

Abstract Objectives: After axotomy, Schwann cells (SCs), required for successful nerve regeneration, undergo a number of cellular changes including dedifferentiation, proliferation, expression of molecules that support axon growth, and apoptosis. This study investigated the role of p75 NTR , sortilin, and proneurotrophins in SC survival after facial nerve (FN) axotomy. Study Design: Preliminary animal study. Methods: With use of FN SCs, expression of p75 NTR and its coreceptor sortilin were quantified by immunofluorescence on days 12, 22, and 52 after axotomy in vivo and by Western blot in vitro. Contralateral FNs served as a control. SC apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL). To verify a causative role for p75 NTR in FN SC death, cultured FN SCs were treated with pro‐nerve growth factor (NGF), and apoptosis was determined by TUNEL. Results: Expression of p75 NTR and sortilin increased in FN SCs distal ( P < .05) to the axotomy compared with the contralateral controls for all time points. SC apoptosis also significantly increased in the distal segment compared with the contralateral and proximal portions ( P < .05). ProNGF, a p75 NTR ligand, increased apoptosis and p75 NTR expression in primary FN SC cultures. Conclusion: FN axotomy increases p75 NTR and sortilin expression in SCs, which correlates with increased apoptosis. These findings suggest roles for p75 NTR and sortilin in SC loss after FN injury. Sortilin is a novel target in promoting FN healing after injury.

Axotomy

Terminal deoxynucleotidyl transferase

Nerve Injury

10.1097/mlg.0b013e31814b8d9f

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Citations (30)

Bone cements: review of their physiochemical and biochemical properties in percutaneous vertebroplasty.

Matthew J. Provenzano Kieran Murphy Lee H. Riley

Percutaneous vertebroplasty is an effective treatment for aggressive vertebral hemangiomas, osteoporotic vertebral compression fractures, spinal metastases, and myelomas. As percutaneous vertebroplasty is more commonly performed to treat various forms of back pain, new or modified cements are being used. This review examines the physiochemical and biomechanical properties of various bone cements and additives.

Percutaneous Vertebroplasty

Bone cement

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Citations (66)

p75NTR expression and nuclear localization of p75NTR intracellular domain in spiral ganglion Schwann cells following deafness correlate with cell proliferation

Molecular and Cellular Neuroscience (2011)

Matthew J. Provenzano Sarah A. Minner Kaitlin Zander J. Jason Clark Catherine J. Kane

Spiral ganglion

10.1016/j.mcn.2011.05.010

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Citations (44)

What is the best method to treat CSF leaks following resection of an acoustic neuroma?

The Laryngoscope (2014)

Matthew J. Provenzano Daniel Choo

10.1002/lary.24157

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Citations (2)

Is ventilation tube placement beneficial during tympanoplasty for atelectatic tympanic membranes?

The Laryngoscope (2014)

Matthew J. Provenzano Daniel Choo

10.1002/lary.24206

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Citations (0)

The ErbB Inhibitors Trastuzumab and Erlotinib Inhibit Growth of Vestibular Schwannoma Xenografts in Nude Mice

Otology & Neurotology (2008)

J. Jason Clark Matthew J. Provenzano Henry R. Diggelmann Ningyong Xu Skylar S. Hansen

To analyze the ability of ErbB inhibitors to reduce the growth of vestibular schwannoma (VS) xenografts.Vestibular schwannoma xenografts were established in the interscapular fat pad in nude mice for 4 weeks. Initially, a small cohort of animals was treated with the ErbB2 inhibitor trastuzumab or saline for 2 weeks. Animals also received bromodeoxyuridine injections to label proliferating cells. In a longer-term experiment, animals were randomized to receive trastuzumab, erlotinib (an ErbB kinase inhibitor), or placebo for 12 weeks. Tumor growth was monitored by magnetic resonance imaging during the treatment period. Cell death was analyzed by terminal deoxynucleotidyl transferase-mediated dUTP-biotin end labeling of fragmented DNA.Tumors can be distinguished with T2-weighted magnetic resonance imaging sequences. Trastuzumab significantly reduced the proliferation of VS cells compared with control (p < 0.01) as analyzed by bromodeoxyuridine uptake. Control tumors demonstrated slight growth during the 12-week treatment period. Both trastuzumab and erlotinib significantly reduced the growth of VS xenografts (p < 0.05). Erlotinib, but not trastuzumab, resulted in a significant increase in the percentage of terminal deoxynucleotidyl transferase-mediated dUTP-biotin end labeling of fragmented DNA-positive VS cells (p < 0.01).In this preliminary study, the ErbB inhibitors trastuzumab and erlotinib decreased growth of VS xenografts in nude mice, raising the possibility of using ErbB inhibitors in the management of patients with schwannomas, particularly those with neurofibromatosis Type 2.

Terminal deoxynucleotidyl transferase

Erlotinib Hydrochloride

Bromodeoxyuridine

10.1097/mao.0b013e31817f7398

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Citations (51)

A Novel Low Cost Task Trainer for Tonsillectomy Simulation

Otolaryngology (2012)

Eric Gantwerker Péter Tóth Matthew J. Provenzano Jareen Meinzen‐Derr Charles M. Myer

Objective 1) Develop a novel low cost task trainer which appropriately simulates the basic steps involved in a tonsillectomy procedure for the novice trainee. 2) Assess this task trainer for feasibility, face validity, usability, and content validity using a group of expert observers. Method The task trainer was developed to reproduce all steps of the tonsillectomy procedure using pieces of steak with adherent connective tissue to simulate the tonsil for electrocautery dissection. Pediatric otolaryngology attendings and fellows were surveyed using a 5‐point Likert scale to assess ease of use, realism, and perceived utility. Results The model consists of a Styrofoam head with cut‐out oropharynx and steak pieces suspended by metal clips and wired to an electrocautery unit. Surgeons use a headlight, grasp the muscle, and electro‐dissect it away from the fat. Cost of the model was twenty US dollars. Ten attending pediatric otolaryngologists and 4 pediatric otolaryngology fellows completed simulated tonsillectomies and surveys. Participants rated a median realism score of 4 out of 5 (very realistic) and ease of use a median 5 out of 5 (simple and intuitive). All participants agreed residents would be better prepared for real tonsillectomies after using the model. Conclusion Production and use of a tonsillectomy task trainer is a feasible and inexpensive endeavor for training programs and may provide appropriate usability and fidelity in simulating tonsillectomy for trainees. Face and content validity were confirmed by expert observers. Further study will assess validity and effectiveness for teaching and assessment.

Trainer

Face validity

Heuristics

System usability scale

Content Validity

10.1177/0194599812451426a18

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Citations (0)

Pediatric endoscopic airway management with posterior cricoid rib grafting

The Laryngoscope (2011)

Matthew J. Provenzano Stephanie L. Hulstein Donald H. Solomon Nancy M. Bauman José M. Manaligod

Abstract Objectives/Hypothesis: To confirm and extend reported successful treatment of posterior glottic stenosis in pediatric patients using endoscopic laser division of the posterior cricoid plate with augmentation using costal cartilage. Study Design: A retrospective chart review and case series. Methods: Medical records were examined to determine the surgical indications, outcomes, and postoperative complications of this procedure. Results: Twelve patients underwent the procedure, six females and six males, with an average age of 7 years (range, 2–26 years). There were 8/12 (67%) patients successfully decannulated after being tracheostomy dependent. There were no consistent anatomic abnormalities or surgical findings predictive of failure to decannulate. Average hospital stay was 3.6 days (range, 2–9 days). There were no deaths or other major complications; one patient had extrusion. Conclusions: Endoscopic posterior cricoid grafting is a valuable surgical option for patients with posterior glottic stenosis. The procedure is associated with low morbidity and permits decannulation in the majority of patients.

Cricoid cartilage

Subglottic stenosis

Medical record

10.1002/lary.21579

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Citations (22)

Use of the King LT for Emergency Airway Management

Archives of Otolaryngology - Head and Neck Surgery (2010)

Sobia F. Khaja Matthew J. Provenzano Kristi Chang

Objective

To discuss the role of the King LT reusable supraglottic airway in emergency airway management.

Design

Retrospective case series review.

Setting

Tertiary academic medical facility.

Patients

We studied patients who presented to the emergency trauma center having undergone intubation at an outside facility or at the scene of the incident. The otolaryngology service was consulted for definitive management of the airway.

Main Outcome Measure

Airway evaluation and management once the King LT has been placed.

Results

Six patients with known prehospitalization use of the King LT presented to the emergency trauma center and subsequently required emergency tracheostomy for establishment of a secure airway. Fiberoptic and/or direct laryngoscopic evaluation performed with the tube in place failed to reveal whether safe oral endotracheal intubation could be performed because of visualization problems. Examination after tracheostomy and removal of the King LT revealed that in 2 patients, orotracheal intubation would have been difficult or impossible, whereas another 4 patients could have been intubated. One patient had prehospitalization placement of a King LT, which resulted in subcutaneous emphysema because of placement within the mediastinum. The patient was able to be successfully intubated and did not require tracheostomy.

Conclusions

The King LT offers benefits in emergency situations, but evaluation of the airway is challenging and often necessitates tracheostomy for establishment of a safe and secure airway. Even if tracheostomy is not required, serious complications may occur.

10.1001/archoto.2010.172

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Citations (10)

Human Chondrosarcoma Cells Acquire an Epithelial-Like Gene Expression Pattern via an Epigenetic Switch: Evidence for Mesenchymal-Epithelial Transition during Sarcomagenesis

Matthew P. Fitzgerald Françoise A. Gourronc Melissa L.T. Teoh Matthew J. Provenzano Adam J. Case

Chondrocytes are mesenchymally derived cells that reportedly acquire some epithelial characteristics; however, whether this is a progression through a mesenchymal to epithelial transition (MET) during chondrosarcoma development is still a matter of investigation. We observed that chondrosarcoma cells acquired the expression of four epithelial markers, E-cadherin,desmocollin 3, maspin, and 14-3-3σ, all of which are governed epigenetically through cytosine methylation. Indeed, loss of cytosine methylation was tightly associated with acquired expression of both maspin and 14-3-3σ in chondrosarcomas. In contrast, chondrocyte cells were negative for maspin and 14-3-3σ and displayed nearly complete DNA methylation. Robust activation of these genes was also observed in chondrocyte cells following 5-aza-dC treatment. We also examined the transcription factor snail which has been reported to be an important mediator of epithelial to mesenchymal transitions (EMTs). In chondrosarcoma cells snail is downregulated suggesting a role for loss of snail expression in lineage maintenance. Taken together, these results document an epigenetic switch associated with an MET-like phenomenon that accompanies chondrosarcoma progression.

10.1155/2011/598218

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Citations (39)