Immediately after far (254) nm and near (300--380 nm) UV light in small and moderate doses alcian blue sorption by glycocalix of Zaidela ascitic hepatoma cells decrease, which is indicative of destruction and solubilization. The effect of UV light on the cell surface is compared with the action of trypsin. Contribution of the damage of outer perimembrane layers to the lethal effect of UV light is discussed.
The outer perimembrane layer of murine spleen lymphocytes were studied with electron microscopy. Mice were treated with short-wave (254 nm) and long-wave (365 nm) ultraviolet radiation in isoeffective lethal doses. The other perimembrane layers were stained with Alcian blue and Ruthenium red. The 254 nm UV treatment decreased the level of dye--sorption by these perimembrane layers, whereas no such effect was obvious after the 365 nm UV treatment.
The use of the method of autologous blood photomodification in surgery is discussed on basis of generalized experience of the Leningrad Research-Practical Center of Blood Photomodification (3,000 procedures conducted in over 2,000 patients). The current state of the problem, the main mechanisms of the therapeutic action of the procedure, and the clinical efficacy of the method in various surgical diseases are dealt with. The possible complications and the means of their prevention are deal with. The methodological aspects of research are discussed. The authors define the main problems which must be solved in order to raise the efficacy of using photomodification of autologous blood in surgery.
Potentialities for evaluating the viability of the Zaidela ascitic hepatoma cells by the number of tumours formed after transplantation of cells to the rat's eye anterior chamber have been considered. Dose effect of far (254 nm) and near (300--380 nm) UV light on the cell tumoregenecity has been studied.
An experimental analysis of various methods of photomodification of blood has shown that their use in medical practice has principle possibility to obtain effects of various directions at the expense of variation of blood irradiation regimens. UV radiation brings about processes of lipid peroxidation in plasma membrane of lymphocytes, their intensity depends on the presence of oxygen in the surroundings.
Cells of the Unio tumidus gill ciliated epithelium were exposed to a supraoptimal temperature of 40 degrees C for the period from 30 seconds till a complete cessation of ciliar beating. ATPase activity of mitochondria of these cells being checked against their size during the corresponding period. The mitochondria were found to change their size in a phasic manner, swelling at the beginning of the temperature exposure, then shrinking and then swelling again. The ATPase activity increased during the first swelling and during shrinking to the control level. The data obtained indicate that similar ultrastructure and size of mitochondria may correspond to different levels of their functional activity.
