Francis Lacombe

University of Waterloo

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Françis Belloc

Inserm

Josy Reiffers

Université de Bordeaux

Marie C. Béné

Nantes Université

Patrice Dumain

Hôpital Cardiologique du Haut-Lévêque

M Boisseau

Hôpital Cardiologique du Haut-Lévêque

Philippe Bernard

Université de Reims Champagne-Ardenne

Gérald Marit

Université de Bordeaux

Josy Reiffers

Institut Bergonié

Jean Vial

Centre Hospitalier Universitaire de Bordeaux

M. Puntous

Centre Hospitalier Universitaire de Bordeaux

Cooperative Institutions

Inserm

106

Hôpital Cardiologique du Haut-Lévêque

Université de Bordeaux

Centre National de la Recherche Scientifique

Centre Hospitalier Universitaire de Bordeaux

Assistance Publique – Hôpitaux de Paris

Bordeaux Population Health

Hôpital Pellegrin

Biotherapy of Genetic Diseases, Inflammatory Disorders and Cancers

Massachusetts General Hospital

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Flow cytometric study of apoptosis by DNA content analysis and cell sorting

Biology of the Cell (1992)

Françis Belloc Francis Lacombe Patrice Dumain Lopez Francis Bernard Philippe

10.1016/0248-4900(92)90327-w

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Citations (0)

Increased liposome‐mediated gene transfer into haematopoietic cells grown in adhesion to stromal or fibroblast cell line monolayers

European Journal Of Haematology (2000)

Gérald Marit Yi Cao Patrick Froussard Jean Ripoche Maryse Dupouy

Abstract: We investigated transfection rates of CD34 + hematopoietic progenitor cells (HPC) or haematopoietic cell lines (TF‐1, KG1a and K562) using the LacZ gene as a reporter and cationic liposomes. The transfection efficiency of CD34 + hematopoietic progenitor cells (HPC) or TF‐1, KG1a and K562 grown in suspension is very low (average percentage of 0.013 for HPC and 0,03 for cell lines). Adhesion of HPC or cell lines to plates by immunological or physical methods significantly enhances transfection efficiency; however, the percentage of transfected cells still remained low. We found that adhesion of TF‐1, KG1a and K562 HC to MS‐5 stroma cells or NIH‐3T3 fibroblast cells increased transfection efficiency. Under these conditions transfection is achieved in 11.2–25% (mean 18.3%) for the cell lines and 13.6% (range 8.2%–24.2%) for CD34 + HPC. These results indicate that liposome‐mediated transfection of HC is significantly increased when cells are grown in adherence to stroma or fibroblast monolayers.

K562 cells

10.1034/j.1600-0609.2000.80230.x

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Citations (16)

Caspase Activation Is an Early Event in Anthracycline-Induced Apoptosis and Allows Detection of Apoptotic Cells before They Are Ingested by Phagocytes

Experimental Cell Research (1998)

Françoise Durrieu Françis Belloc L. Lacoste Patrice Dumain J. Chabrol

10.1006/excr.1997.3918

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Citations (53)

Unsupervised Flow Cytometry Analysis Allows for an Accurate Identification of Minimal Residual Disease Assessment in Acute Myeloid Leukemia

Cancers (2021)

Jean Vial Nicolas Lechevalier Francis Lacombe Pierre‐Yves Dumas Audrey Bidet

The assessment of minimal residual disease (MRD) is increasingly considered to monitor response to therapy in hematological malignancies. In acute myeloblastic leukemia (AML), molecular MRD (mMRD) is possible for about half the patients while multiparameter flow cytometry (MFC) is more broadly available. However, MFC analysis strategies are highly operator-dependent. Recently, new tools have been designed for unsupervised MFC analysis, segregating cell-clusters with the same immunophenotypic characteristics. Here, the Flow-Self-Organizing-Maps (FlowSOM) tool was applied to assess MFC-MRD in 96 bone marrow (BM) follow-up (FU) time-points from 40 AML patients with available mMRD. A reference FlowSOM display was built from 19 healthy/normal BM samples (NBM), then simultaneously compared to the patient's diagnosis and FU samples at each time-point. MRD clusters were characterized individually in terms of cell numbers and immunophenotype. This strategy disclosed subclones with varying immunophenotype within single diagnosis and FU samples including populations absent from NBM. Detectable MRD was as low as 0.09% in MFC and 0.051% for mMRD. The concordance between mMRD and MFC-MRD was 80.2%. MFC yielded 85% specificity and 69% sensitivity compared to mMRD. Unsupervised MFC is shown here to allow for an easy and robust assessment of MRD, applicable also to AML patients without molecular markers.

Minimal Residual Disease

Identification

10.3390/cancers13040629

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Citations (28)

FLOW CYTOMETRY AND DETECTION OF ARA‐C RESISTANT LEUKEMIC CELLS

Biology of the Cell (1991)

Francis Lacombe Françis Belloc Patrice Dumain M. Puntous Francis Lopez

10.1016/0248-4900(91)90224-b

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Citations (6)

Minimal/measurable residual disease in AML: a consensus document from the European LeukemiaNet MRD Working Party

Blood (2018)

Gerrit Jan Schuurhuis Michael Heuser Sylvie Freeman Marie C. Béné Francesco Buccisano

Minimal Residual Disease

Hematopathology

Molecular diagnostics

10.1182/blood-2017-09-801498

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Citations (907)

Technical Aspects of Flow Cytometry-based Measurable Residual Disease Quantification in Acute Myeloid Leukemia: Experience of the European LeukemiaNet MRD Working Party

HemaSphere (2021)

Jesse M. Tettero Sylvie Freeman Veit L. Buecklein Adriano Venditti Luca Maurillo

Measurable residual disease (MRD) quantified by multiparameter flow cytometry (MFC) is a strong and independent prognostic factor in acute myeloid leukemia (AML). However, several technical factors may affect the final read-out of the assay. Experts from the MRD Working Party of the European LeukemiaNet evaluated which aspects are crucial for accurate MFC-MRD measurement. Here, we report on the agreement, obtained via a combination of a cross-sectional questionnaire, live discussions, and a Delphi poll. The recommendations consist of several key issues from bone marrow sampling to final laboratory reporting to ensure quality and reproducibility of results. Furthermore, the experiences were tested by comparing two 8-color MRD panels in multiple laboratories. The results presented here underscore the feasibility and the utility of a harmonized theoretical and practical MFC-MRD assessment and are a next step toward further harmonization.

Minimal Residual Disease

Harmonization

Delphi Method

10.1097/hs9.0000000000000676

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Citations (54)

Study of the apoptosis induced in vitro by antitumoral drugs on leukaemic cells

Leukemia Research (1997)

Jean Vial Françis Belloc Patrice Dumain Sabine Besnard Francis Lacombe

IC50

10.1016/s0145-2126(96)00102-6

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