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Phatthranit Phattharapornjaroen

Ramathibodi Hospital

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i-10 index

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Co-Authors

Tanittha Chatsuwan

Chulalongkorn University

Parichart Hongsing

Mae Fah Luang University

Shuichi Abe

Yamagata Prefectural Central Hospital

Dhammika Leshan Wannigama

Thai Red Cross Society

Paul G. Higgins

German Center for Infection Research

Mohan Amarasiri

Yamagata Prefectural Central Hospital

Amir Khorram‐Manesh

University of Gothenburg

Eric Carlström

University of Gothenburg

Cameron Hurst

Charles Darwin University

Asada Leelahavanichkul

Chulalongkorn University

Cooperative Institutions

Mahidol University

Chulalongkorn University

Ramathibodi Hospital

King Chulalongkorn Memorial Hospital

Thai Red Cross Society

Yamagata Prefectural Central Hospital

Perth Children's Hospital

Princess Margaret Hospital for Children

University of Otago

University of Gothenburg

Author Statistics

Papers

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Overcoming addition of phosphoethanolamine to lipid A mediated colistin resistance in Acinetobacter baumannii clinical isolates with colistin–sulbactam combination therapy

Scientific Reports (2022)

Sukrit Srisakul Dhammika Leshan Wannigama Paul G. Higgins Cameron Hurst Shuichi Abe

Overcoming colistin-resistant Acinetobacter baumannii (CoR-AB) has become a major concern due to the lack of effective antibiotics. This study aimed to explore the prevalence of CoR-AB clinical isolates in Thailand, their mechanisms of resistance, and test the efficacy of colistin plus sulbactam against CoR-AB isolates. The colistin resistance rate among carbapenem-resistant A. baumannii was 15.14%. The mcr gene or its variants were not detected in CoR-AB isolates by PCR screening. The lipid A mass spectra of CoR-AB isolates showed the additional [M-H]- ion peak at m/z = 2034 that correlated to the phosphoethanolamine (pEtN) addition to lipid A (N = 27/30). The important amino acid substitutions were found at position S14P, A138T, A227V in PmrB that are associated with overexpression of the pEtN transferase (PmrC) and contributed the pEtN addition. The lipopolysacccharide production genes (lpxACD) were not related to lipid A mass spectra. A colistin plus sulbactam combination exhibited the synergy rate at 86.7% against CoR-AB isolates compare to sulbactam (85.89% resistance) or colistin (15.14% resistance) alone. The excellent synergistic activity of colistin plus sulbactam combination has the potential for the treatment of CoR-AB infections.

Colistin

Acinetobacter baumannii

Sulbactam

Carbapenem

Lipid A

10.1038/s41598-022-15386-1

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Citations (32)

Novel colistin-EDTA combination for successful eradication of colistin-resistant Klebsiella pneumoniae catheter-related biofilm infections

Scientific Reports (2021)

Aye Mya Sithu Shein Dhammika Leshan Wannigama Paul G. Higgins Cameron Hurst Shuichi Abe

Abstract Development of an effective therapy to overcome colistin resistance in Klebsiella pneumoniae , a common pathogen causing catheter-related biofilm infections in vascular catheters, has become a serious therapeutic challenge that must be addressed urgently. Although colistin and EDTA have successful roles for eradicating biofilms, no in vitro and in vivo studies have investigated their efficacy in catheter-related biofilm infections of colistin-resistant K. pneumoniae . In this study, colistin resistance was significantly reversed in both planktonic and mature biofilms of colistin-resistant K. pneumoniae by a combination of colistin (0.25–1 µg/ml) with EDTA (12 mg/ml). This novel colistin-EDTA combination was also demonstrated to have potent efficacy in eradicating colistin-resistant K. pneumoniae catheter-related biofilm infections, and eliminating the risk of recurrence in vivo. Furthermore, this study revealed significant therapeutic efficacy of colistin-EDTA combination in reducing bacterial load in internal organs, lowering serum creatinine, and protecting treated mice from mortality. Altered in vivo expression of different virulence genes indicate bacterial adaptive responses to survive in hostile environments under different treatments. According to these data discovered in this study, a novel colistin-EDTA combination provides favorable efficacy and safety for successful eradication of colistin-resistant K. pneumonia catheter-related biofilm infections.

Colistin

10.1038/s41598-021-01052-5

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Citations (34)

Bag-Valve Mask versus Endotracheal Intubation in Out-of-Hospital Cardiac Arrest on Return of Spontaneous Circulation: A National Database Study

DOAJ (DOAJ: Directory of Open Access Journals) (2020)

Chaiyaporn Yuksen Phatthranit Phattharapornjaroen Woranee Kreethep Chonnakarn Suwanmano Chetsadakon Jenpanitpong

Endotracheal intubation

Circulation (fluid dynamics)

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Citations (1)

Home isolation system using telehealth and community engagement

Harvard Dataverse (2021)

Phatthranit Phattharapornjaroen

The number of patients in home isolation system and number of volunteers CSCATTT evaluation information

Telehealth

Isolation

10.7910/dvn/fuup8l

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Citations (0)

Novel intranasal phage-CaEDTA-ceftazidime/avibactam triple combination therapy demonstrates remarkable efficacy in treating Pseudomonas aeruginosa lung infection

Biomedicine & Pharmacotherapy (2023)

Aye Mya Sithu Shein Dhammika Leshan Wannigama Cameron Hurst Peter N. Monk Mohan Amarasiri

Given the rise of multidrug-resistant (MDR) Pseudomonas aeruginosa infections, alternative treatments are needed. Anti-pseudomonal phage therapy shows promise, but its clinical application is limited due to the development of resistance and a lack of biofilm penetration. Recently, adjuvants like CaEDTA have shown the ability to enhance the effectiveness of combined antimicrobial agents. Here, we tested a phage-adjuvant combination and demonstrated the effectiveness of intranasally inhaled phage (KKP10) + CaEDTA in addition to ceftazidime/avibactam (CZA) for chronic P. aeruginosa lung infections. The results emphasize that intranasal inhalation of phage along with CaEDTA can successfully re-sensitize MDR P. aeruginosa to CZA in a triple combination treatment. This promising approach shows potential as a therapy for chronic respiratory tract infections.

Ceftazidime/avibactam

Phage therapy

10.1016/j.biopha.2023.115793

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Citations (9)

Association between Mode of Transport and Patient Outcomes in the Emergency Department following Out-of-Hospital Cardiac Arrest: A Single-Center Retrospective Study

Prehospital Emergency Care (2022)

Pitsucha Sanguanwit Kanthicha Sutthisuwan Phatthranit Phattharapornjaroen Malivan Phontabtim Yahya Mankong

Background Out-of-hospital cardiac arrest (OHCA) remains a health problem worldwide, carrying a high mortality rate. Comparison of emergency department (ED) return of spontaneous circulation (ROSC) after OHCA in relation to emergency medical services (EMS) and non-EMS modes of transportation to the hospital was conducted to assess the impact points of the EMS system in Thailand.Methods This retrospective observational study enrolled all OHCA patients who visited the ED of Ramathibodi Hospital, a tertiary university hospital in Bangkok, between January 1, 2008, and May 31, 2020. Patients were differentiated into EMS and non-EMS groups according to mode of transportation to the ED. Patients' characteristics and comorbidities, witnessed arrests, bystander chest compression, initial rhythm, and resuscitation treatment were documented. ED-sustained ROSC, ED survival, 30-day survival, and 30-day survival with good cerebral performance category (CPC) scores were monitored and recorded. Multivariate logistic analyses were performed to assess factors influencing clinical outcomes.Results A total of 339 patients were enrolled, 117 (34.51%) of whom were in the EMS transport group. There were no differences between the EMS and non-EMS groups in ED-sustained ROSC (adjusted odds ratio [aOR], 0.99; 95% confidence interval [CI], 0.58–1.70; P = 0.98), or ED survival (aOR, 0.99; 95% CI, 0.57–1.71; P = 0.97). There were also no differences in 30-day survival or 30-day survival with good CPC score between the two groups.Conclusions In our cohort data of OHCA, ED-sustained ROSC and ED survival outcomes were not superior in the EMS transportation group. Evidence to show that EMS transportation affected 30-day survival and 30-day good CPC score was also lacking. Thus, public promotion of Thailand's EMS system is advocated with a simultaneous improvement of EMS response to enhance OHCA outcomes.

10.1080/10903127.2022.2058131

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Alternative Leadership in Flexible Surge Capacity—The Perceived Impact of Tabletop Simulation Exercises on Thai Emergency Physicians Capability to Manage a Major Incident

Sustainability (2020)

Phatthranit Phattharapornjaroen Viktor Glantz Eric Carlström Lina Dahlén Holmqvist Amir Khorram‐Manesh

Flexible surge capacity aims to activate and utilize other resources than normally are surged in a community during the primary and secondary surge capacity. The presence of alternative leadership, skilled and knowledgeable in hospital and prehospital emergency management, is invaluable. Thai emergency physicians work at both levels, emphasizing their important role in emergency management of any source in a disaster-prone country. We aimed to investigate Thai emergency physicians’ ability in terms of knowledge and preparedness to manage potential emergencies using tabletop simulation exercises. Using an established method for training collaboration, two training courses were arranged for over 50 Thai emergency physicians, who were divided into three teams of prehospital, hospital, and incident command groups. Three scenarios of a terror attack along with a bomb explosion, riot, and shooting, and high building fire were presented, and the participants’ performance was evaluated regarding their preparedness, response and gained knowledge. Two senior observers followed the leadership characteristic in particular. Thai physicians’ perceived ability in command and control, communication, collaboration, coordination, and situation assessment improved in all groups systematically. New perspectives and innovative measures were presented by participants, which improved the overall management on the final day. Tabletop simulation exercise increased the perceived ability, knowledge, and attitude of Thai emergency physicians in managing major incidents and disasters. It also enabled them to lead emergency management in a situation when alternative leadership is a necessity as part of the concept of a flexible surge capacity response system.

Preparedness

Surge Capacity

Capacity Building

Mass-casualty incident

Emergency Response

10.3390/su12156216

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Citations (16)

The implication of a translational triage tool in mass casualty incidents: part three: a multinational study, using validated patient cards

Scandinavian Journal of Trauma Resuscitation and Emergency Medicine (2023)

Amir Khorram‐Manesh Eric Carlström Frederick M. Burkle Krzysztof Goniewicz Lesley Gray

Abstract Background Mass casualty incidents (MCI) pose significant challenges to existing resources, entailing multiagency collaboration. Triage is a critical component in the management of MCIs, but the lack of a universally accepted triage system can hinder collaboration and lead to preventable loss of life. This multinational study uses validated patient cards (cases) based on real MCIs to evaluate the feasibility and effectiveness of a novel Translational Triage Tool (TTT) in primary triage assessment of mass casualty victims. Methods Using established triage systems versus TTT, 163 participants (1575 times) triaged five patient cases. The outcomes were statistically compared. Results TTT demonstrated similar sensitivity to the Sieve primary triage method and higher sensitivity than the START primary triage system. However, the TTT algorithm had a lower specificity compared to Sieve and higher over-triage rates. Nevertheless, the TTT algorithm demonstrated several advantages due to its straightforward design, such as rapid assessment, without the need for additional instrumental interventions, enabling the engagement of non-medical personnel. Conclusions The TTT algorithm is a promising and feasible primary triage tool for MCIs. The high number of over-triages potentially impacts resource allocation, but the absence of under-triages eliminates preventable deaths and enables the use of other personal resources. Further research involving larger participant samples, time efficiency assessments, and real-world scenarios is needed to fully assess the TTT algorithm's practicality and effectiveness in diverse multiagency and multinational contexts.

Triage

Mass-casualty incident

Mass Casualty

10.1186/s13049-023-01128-3

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Citations (4)

Tracing the transmission of mpox through wastewater surveillance in Southeast Asia

Journal of Travel Medicine (2023)

Dhammika Leshan Wannigama Mohan Amarasiri Phatthranit Phattharapornjaroen Cameron Hurst Charin Modchang

High population density and tourism in Southeast Asia increase the risk of mpox due to frequent interpersonal contacts. Our wastewater surveillance in six Southeast Asian countries revealed positive signals for Monkeypox virus (MPXV) DNA, indicating local transmission. This alerts clinicians and helps allocate resources like testing, vaccines and therapeutics in resource-limited countries.

Contact tracing

Monkeypox

10.1093/jtm/taad096

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Citations (15)

Chest depth single center

Harvard Dataverse (2022)

Phatthranit Phattharapornjaroen

The raw data from single center.

Center (category theory)

Single Center

10.7910/dvn/8iu34x

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Citations (0)