María del Carmen Castillo‐Hernández

Instituto Politécnico Nacional

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Gustavo Guevara‐Balcázar

Instituto Politécnico Nacional

Alexander Kormanovski

Instituto Politécnico Nacional

Eleazar Lara‐Padilla

Instituto Politécnico Nacional

Antonio Franco‐Vadillo

Instituto Politécnico Nacional

Mireille Toledo-Blas

Instituto Politécnico Nacional

Fidelina Parra

Instituto Politécnico Nacional

Nallely Acevedo‐Villavicencio

Instituto Politécnico Nacional

Israel Ramírez‐Sánchez

Instituto Politécnico Nacional

Maria T. Perez

Hospital Universitario de La Princesa

Pedro López‐Sánchez

Instituto Politécnico Nacional

Cooperative Institutions

Instituto Politécnico Nacional

Tecnológico Nacional de México

Mexican Social Security Institute

Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

Center for Research and Advanced Studies of the National Polytechnic Institute

Universidad Nacional Autónoma de México

Universidad Insurgentes

Centro Medico Nacional Siglo XXI

Hospital Médica Sur

Hospital de Especialidades

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Cannabidiol‐mediated RISK PI3K/AKT and MAPK/ERK pathways decreasing reperfusion myocardial damage

Pharmacology Research & Perspectives (2021)

Antonio Franco‐Vadillo Mireille Toledo‐Blass Zeltzin Rivera‐Herrera Gustavo Guevara‐Balcázar Oscar Orihuela

Abstract Myocardial ischemia continues to be the first cause of morbimortality in the world; the definitive treatment is reperfusion; however, this action causes additional damage to ischemic myocardial tissue; this forces to seek therapies of cardioprotection to reduce this additional damage. There are many cardioprotective agents; within these, cannabinoids have shown to have beneficial effects, mainly cannabidiol (CBD). CBD is a non psychoactive cannabinoid. To evaluate the effect in experimental models of CBD in myocardial ischemia reperfusion in rats, twelve‐week‐old male rats have been used. The animals were divides in 3 groups: control(C), ischemia reperfusion (IR) and CBD pretreatment (1/day/5mg/kg /10days). Langendorff organ isolate studies were performed, and the area of infarction was assessed with triphenyl tetrazolium, in addition to molecular analysis of AT1 and AT2 receptors and Akt and Erk proteins and their phosphorylated forms related to RISK pathways. It was observed that there is an improvement with the use of CBD increasing inotropism and cardiac lusitropism, improving considerably the cardiovascular functionality. These could be related to the reduction of the area of infarction and activation of the AT2 receptor and the RISK pathway with absence of activation of the AT2 receptor (these could relate the reduction of the infarct area and the restoration of cardiovascular function with the activation of the AT2 receptor and the RISK pathway with the absence of activation of the AT2 receptor). The use of cannabinoids was shown to have beneficial effects when used as a treatment for myocardial reperfusion damage.

Cardioprotection

10.1002/prp2.784

Cite

Citations (22)

Oxidative stress and vascular reactivity changes in thoracic and abdominal aorta expose at the hyperbaric oxygenation (693.1)

The FASEB Journal (2014)

Nallely Acevedo‐Villavicencio Alexander Kormanovsky Cleva Villanueva Israel Ramírez‐Sánchez Eleazar Lara‐Padilla

During the hyperbaric oxygenation therapy (HBO) has been described some changes like hypoglycemia, low blood pressure, etc. The mean idea of this work is to analyze if the HBO induce some changes in the vascular reactivity and oxidative stress in healthy rats. Methods: Male rats were used (3 months old) and divided in 2 groups: with and without HBO.Rats were exposed to pure oxygen at 2 absolute atmospheres during 60 min for 3, 5, 10 and 20 sessions, after that, we obtain thoracic and abdominal aorta and were used some of the aortic rings for the isolated organ system where we made concentration response curves; and other aortic rings were used to analyze the oxidative stress. Results: changes were observed in the contractile effect to phenyleprine in both segments after HBO, without any changes in the relaxant response. On the other hand thoracic oxidative response were higher after 3 sessions of HBO, but in an interesting way we can observe that antioxidant enzyme decreased after this time; those results suggest that the antioxidant system increase the response trying to metabolize O2. Grant Funding Source : COFAA / IPN

Abdominal aorta

Thoracic aorta

Hyperbaric oxygenation

Reactivity

10.1096/fasebj.28.1_supplement.693.1

Cite

Citations (0)

Changes during development of COX 1 and 2 in heart, abdominal and thoracic aorta (837.11)

The FASEB Journal (2014)

Nallely Acevedo‐Villavicencio Mónica Arellano Israel Ramírez‐Sánchez Elvia Mera‐Jimenez Emilio Lopez‐Calderon

Cyclooxigenase 1 and 2 have been related in the release of prostanoids that regulate the effect of various agonists as in the case of phenylephrine, but its unknown that how this enzyme is involved in the contractile response to phenylephrine age‐dependent. The aim of this paper is to analyze changes of COX 1 and 2 involvements in the contractile response to phenylephrine in the thoracic and abdominal aorta and coronary artery of pubescent, young and old rats. It has been describe that during development there are changes in the activity of some enzymatic pathways, in this work we analyze the change in the involvement of cyclooxygenase and PGs in the contractile effect of phenylephrine with age. Methods: we used male rats of 6, 16 weeks and 1 year of age. Rats were anesthetized with pentobarbital (50 mg / kg), after that we proceeded to obtain the thoracic aorta and abdominal aorta and the heart and they were placed in an isolated organ system where we proceeded to make concentration response curves to phenylephrine. On the other hand we obtain heart and aorta for the western blot analysis and the immunohistochemistry assay of COX 1 and COX 2. Conclusions: COX1 increase during development. On the other hand COX2 decrease in the elderly stage only in thoracic aorta. Grant Funding Source : COFAA / IPN

Phenylephrine

Abdominal aorta

Thoracic aorta

10.1096/fasebj.28.1_supplement.837.11

Cite

Citations (0)

Impact of Classical Risk Factors for Arterial or Venous Thrombosis in Patients With Antiphospholipid Syndrome

Clinical and Applied Thrombosis/Hemostasis (2017)

Daniel Navarro-Carpentieri María del Carmen Castillo‐Hernández Karim Majluf‐Cruz Guillermo Espejo-Godinez Paola Itzel Carmona-Olvera

There are classical risk factors associated with arterial thrombosis (AT) or venous thromboembolic disease (VTD). However, less is known about these risk factors and AT or VTD episodes in patients with antiphospholipid syndrome (APS). Our aim was to elucidate whether APS-related thrombotic episodes are associated with the same risk factors as the non-APS population. We gathered demographics, medical history, complications, and causes of death associated with the risk factors for AT or VTD in patients with APS. We analyzed 677 thrombotic events in 386 patients. Type 2 diabetes mellitus and grade 3 obesity were associated with VTD instead of AT. There were no significant differences between the groups for almost all laboratory tests analyzed, although lupus anticoagulant was significantly higher in the VTD group. We suggest that thrombosis in APS is due to the APS itself and that the risks factors for AT or VTD do not have a main role. Our findings may have an ethnical background. Therefore, it may be difficult to elaborate predictive thrombotic clinical scores applicable to patients with different ethnical background.

Lupus anticoagulant

10.1177/1076029617727859

Cite

Citations (11)

Effec of N-Acetylcysteine in conjunctival pterygium

Investigative Ophthalmology & Visual Science (2019)

Fidelina Parra Mireille Toledo-Blas Antonio Franco‐Vadillo Daniel R. Capiz-Correa Alexander Kormanovski-Kovzova

Pterygium

Source

Cite

Citations (0)

Murine model evaluation of biochemical and tissue-damage induced by Bothrops asper (Nauyaca) snake venom

Medicina e Investigación Universidad Autónoma del Estado de México (2025)

Carlos Ángel Gallardo Casas María Teresa Valadez-Omaña Emmanuel Correa Solís María del Carmen Castillo‐Hernández Rocı́o Ortiz-Butrón

Introduction: Venomous snakes Bites are a serious public health issue, and in Mexico, Bothrops asper is responsible for the majority of viper bites. Objective: To evaluate, in a mouse model, the effect of Bothrops asper venom snake on liver enzymes LDH, AST, and ALT, as well as to assess its effect on renal and hepatic histology. Methods: The LD50 was determined using a dose-response curve after intramuscular administration of increasing doses of the venom to mice (0.5, 1, 2.5, 5, and 10 mg of protein/kg). Subsequently, the mice were administered the LD25 value of the venom (or saline solution for control), and 24 hours after the injection, LDH, AST, and ALT enzymes were measured in the blood, and the liver and kidneys were collected from each animal. Results: Results: Animals treated with poison (LD25 of 1 mg of protein/kg) had greater activity of AST, ALT and LDH enzymes, structural damage in the cortex and renal medulla, as well as in the liver after 24 hours of administration in the experimental group Conclusion: B. asper venom causes an increase in LDH and transaminase enzymes, as well as renal and hepatic histopathological alterations.

Bothrops

10.36677/medicinainvestigacion.v13i1.24277

Cite

Citations (0)

Interaction between hyperbaric oxygen therapy and prostaglandin synthesis inhibition in the formalin pain test

The FASEB Journal (2020)

Sheila Belen Cruz-Martinez María del Carmen Castillo‐Hernández Gustavo Guevara‐Balcázar José Correa‐Basurto Diego Alejandro Ríos-Ramírez

Background Pain is the main symptom of inflammatory diseases, such as diabetes mellitus, hypertension, osteoarthritis among others. Lipopolysacaccharide from E. coli (LPS) has been used to induce an inflammatory process. Prostaglandins has a role in inflammation so that NSAIDs are useful pharmacological tools to treat it. Hyperbaric oxygen therapy (HBOT) has also been used in some of these diseases. However, information about pharmacological interaction with HBOT and its mechanism is scarce. So, the purpose of this work was to study the interaction between LPS, HBOT and NSAIDs using a formalin pain test. Methods 96 adults male Wistar rats (150 to 200 g) were used and 2 groups treated with LPS or its vehicle were formed and each of them was divided in the next subgroups (n=8 each): Control, indomethacin, rofecoxib, HBOT, HBOT/indomethacin and HBOT/rofecoxib. 20 μL of 2% formalin was injected into the plantar surface of the right hind paw. Then nociceptive behavior was evaluated as the number of flinches in 1 min every 5 for 60 min. LPS and HBOT were applied 4 and 2.5 h prior to formalin respectively. Additionally, PGE2 and PGI2 was quantified using RP‐HPLC‐UV. Results Formalin produces a nociceptive behavior that decreased in the first 10 min (phase I) and in the next 50 increased to a plateau and then become to reduce (phase II, inflammatory). LPS increased and HBOT and drugs treatment reduced the phase II, while PGI2 levels were increased with LPS and reduced with HBOT and drugs. The HBOT seems to increase the indomethacin antinociceptive effect but HBOT effect was lesser than control and levels of prostaglandins were similar than in absence of HBOT. LPS treatment reduced the rofecoxib and HBOT effect increasing PGE2 and PGI2 levels respectively. Finally, when HBOT is associated with LPS and indomethacin, an increase on AUC of formalin and PGI2 levels was observed. Conclusions The HBOT and prostaglandin synthesis inhibition were effective antinociceptive interventions. However, indomethacin but not rofecoxib increased the HBOT antinoceptive effect diminishing PGI2 levels. In presence of an inflammatory process, the effect of HBOT and rofecoxib but no indomethacin, the antinociception was lost due to an increase in prostaglandin synthesis. Therefore, there are an interaction between HBOT and PG synthesis inhibition and the presence of inflammation reduced the beneficial effects of physical or pharmacological interventions. Support or Funding Information The authors would like to acknowledge CONACYT (557096) for their financial support

10.1096/fasebj.2020.34.s1.05806

Cite

Citations (0)

Protective Role of Glutathione and Nitric Oxide Production in the Pathogenesis of Pterygium

Journal of Ophthalmology (2020)

Fidelina Parra Alexander Kormanovski Gustavo Guevara‐Balcázar María del Carmen Castillo‐Hernández Antonio Franco‐Vadillo

In the pathogenesis of pterygium, the protective role of glutathione and nitric oxide production is unclear. These are important factors for homeostasis in the redox state of cells. The aim of this study was to determine the levels of these and related parameters in pterygium tissue. Patients and Methods. The study sample consisted of 120 patients diagnosed with primary or recurrent pterygium. Five groups of tissue samples were examined: control, primary pterygium, recurrent pterygium, and two groups of primary pterygium given a one-month NAC presurgery treatment (topical or systemic). The levels of endothelial nitric oxide synthase (eNOS), nitric oxide (NO), 3-nitrotyrosine (3NT), reduced and oxidized glutathione (GSH and GSSG), and catalase (CAT) were evaluated in tissue homogenates.Compared with the control, decreased levels of eNOS, NO, and 3-nitrotyrosine as well as the degree of oxidation of GSH (GSSG%) were observed in primary and recurrent pterygium. 3-Nitrotyrosine and GSSG% were reduced in the other pterygium groups. GSH and CAT were enhanced in recurrent pterygium and systemic-treated primary pterygium but were unchanged for topical-treated primary pterygium. There was a strong positive correlation of eNOS with NO and 3NT, GSSG% with NO and 3NT, and GSH with GSSG and CAT. Women showed a higher level of GSH and catalase in primary pterygium, whereas a lower level of GSH and a higher level of NO in recurrent pterygium.The results are congruent with the following proposed sequence of events leading to a protective response of the organism during the pathogenesis of primary pterygium: a decreased level of eNOS provokes a decline in the level of NO in pterygium tissue, which then leads to reduced S-nitrosylation of GSH or other thiols and possibly to the modulation of the intracellular level of GSH through synthesis and/or mobilization from other tissues.

Pterygium

Pathogenesis

Nitrotyrosine

10.1155/2020/9638763

Cite

Citations (2)

Combined high-fat diet and sustained high sucrose consumption promotes NAFLD in a murine model

Annals of Hepatology (2015)

Gonzalo Torres‐Villalobos Nashla Hamdan‐Pérez Armando R. Tovar Guillermo Ordáz‐Nava Braulio Martínez‐Benítez

The study of NAFLD in humans has several limitations. Using murine models helps to understand disease pathogenesis.Evaluate the impact of 4 different diets in the production of NAFLD with emphasis on a combined high-fat plus sustained high sucrose consumption.Eight week-old male Wistar rats were divided in four groups and fed for 90 days with the following diets: 1) Control chow diet (C); 2) High-fat cholesterol diet (HFC) + 5% sucrose in drinking water. 3) High-fat cornstarch diet (HFCO) + 5% sucrose in drinking water. 4) Chow diet + 20% sucrose in drinking water (HSD). Metabolic changes, leptin levels, liver histology, hepatic and plasma lipid composition, fasting plasma glucose and insulin and liver gene expression of FAS, SREBP-1 and PPAR-α were evaluated.The HFC diet had the highest grade of steatosis (grade 2 of 3) and HSD showed also steatosis (grade 1). Liver weight TG and colesterol concentrations in liver were greater in the HFC diet. There were no increased levels of iron in the liver. Rats in HFC gained significantly more weight (P < 0.001). All experimental groups showed fasting hyperglycemia. HFC had the highest glucose level (158.5 ± 7 mg/dL) (P < 0.005). The HSD and the HFCO diets developed also hyperglycemia. HSD had significantly higher fasting hyperinsulinemia. Serum leptin was higher in the HFC diet (p = 0.001). In conclusion, the HFC diet with combination of high fat and high sucrose is more effective in producing NAFLD compared with a high sucrose diet only.

Hyperinsulinemia

Steatosis

Dietary Sucrose

10.1016/s1665-2681(19)31176-7

Cite

Citations (33)

Gender differences in nitric oxide and antioxidant response to physical stress in tissues of trained mice after hyperbaric oxygen preconditioning

Physiology and Pharmacology (2019)

Alexander Kormanovski María del Carmen Castillo‐Hernández Gustavo Guevara‐Balcázar Maria T. Perez Eleazar Lara‐Padilla

Source

Cite

Citations (0)