Lixuan Wu

Fujian Agriculture and Forestry University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Huan Ge

Xiangya Hospital Central South University

Lihui Zeng

Fujian Agriculture and Forestry University

Zhiyu Cai

Fujian Medical University

Xiaojing Huang

Fujian Medical University

Guozhuang Zhang

Chinese Academy of Medical Sciences & Peking Union Medical College

Yuqing Zheng

Shan Jiang

Fritz Haber Institute of the Max Planck Society

Shuangrong Gao

Chinese Academy of Medical Sciences & Peking Union Medical College

Lishan Lei

Fujian Medical University

Shilin Chen

Chinese Academy of Medical Sciences & Peking Union Medical College

Cooperative Institutions

Chinese Academy of Medical Sciences & Peking Union Medical College

Fujian Agriculture and Forestry University

Fujian Medical University

China Academy of Chinese Medical Sciences

Sichuan University

Central South University

Shanghai Jiao Tong University

Chengdu University of Traditional Chinese Medicine

Chinese Academy of Sciences

Huazhong University of Science and Technology

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Pretreatment of Root Canal with Photodynamic Therapy Facilitates Adhesion, Viability and Differentiation of Stem Cells of the Apical Papilla

Photochemistry and Photobiology (2020)

Yijun Li Huan Ge Lixuan Wu Lishan Lei Yanhuang Wang

Abstract This study was to test the hypothesis that root canal pretreated with photodynamic therapy (PDT) would promote stem cells from the apical papilla (SCAP) adhesion, proliferation and differentiation without affecting smear layer removal and microhardness of root canal. Standardized root canals were randomized into four groups ( n = 30/group): (1) sodium hypochlorite(NaOCl) group, (2) NaOCl + ethylene diaminetetraacetic acid (EDTA) group, (3) NaOCl + PDT group, (4) NaOCl + EDTA + PDT group. After treatments, smear layer removal and microhardness of root canal were evaluated. SCAP with hydroxyapatite‐based scaffolds were seeded into root canals for 7 days. SCAP adhesion was observed by scanning electron microscope (SEM), and viable cells were calculated by CellTiter‐Glo Luminescent kit. Platelet‐derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) expression of SCAP were evaluated by Quantitative Reverse Transcriptase‐Polymerase Chain Reaction. There was no significant difference in the smear layer removal and microhardness of root dentin between the groups with and without PDT treatment ( P > 0.05). SCAP with elongated cytoplasmic processes and cell–cell contact were observed on the dentin surfaces treated with PDT. Elevated cell viability, PDGF and VEGF expression were found in root canal treated with PDT ( P < 0.05). Under the experimental conditions, PDT could provide positive microenvironment for SCAP growth.

Sodium hypochlorite

Viability assay

Smear layer

10.1111/php.13240

Cite

Citations (6)

Virus-induced gene silencing (VIGS) in Chinese narcissus and its use in functional analysis of NtMYB3

Horticultural Plant Journal (2021)

Ping Zhou Jiayu Peng Min-Jie Zeng Lixuan Wu Yuxin Fan

Virus-induced gene silencing (VIGS) is a natural defense mechanism of plants, which can cause sequence-specific degradation of viral RNA and is often used for studying the functional genome. At present, the VIGS system mediated by Tobacco rattle virus (TRV) has been successfully established in many plant species. However, the VIGS system in Chinese narcissus had not been reported yet. In this paper, the first use of the TRV-VIGS system in Chinese narcissus is described in detail. By injecting pTRV2-GFP into narcissus leaves, we confirmed that TRV could infect narcissus and move in narcissus plants. After the inoculation of pTRV2-NtPDS by two methods, phenomena such as photo-bleaching appeared in narcissus leaves and qRT-PCR results proved that PDS gene of narcissus was successfully silenced. Besides, pTRV2-NtMYB3 was inoculated into basal plates of narcissus to study the function of NtMYB3; the results showed that NtMYB3 is an inhibitor of flavonoids biosynthesis, which can increase the content of proanthocyanidins in basal plates of narcissus by repressing the expression level of NtFLS. These results indicate that the TRV-VIGS system has been successfully established in Chinese narcissus and successfully applied to the functional study of NtMYB3.

Narcissus

Tobacco rattle virus

10.1016/j.hpj.2021.04.009

Cite

Citations (23)

Modules in robust but low-efficiency phyllosphere fungal networks drive saponin accumulation in leaves of different Panax species

Environmental Microbiome (2023)

Guozhuang Zhang Liping Shi Congsheng Liu Renjun Mao Bing Xia

Abstract Background The phyllosphere mycobiome plays a crucial role in plant fitness and ecosystem functions. The complex microbial ecological networks (MEN) formed by these fungi remain poorly understood, particularly with regard to their organization strategy and their contributions to plant secondary metabolites such as saponin. Results In this study, we constructed six MENs from leaf epiphytic and endophytic mycobiomes of three Panax species distributed in the northeast and southwest ends of mainland China. Hub nodes were absent in these MENs, which were significantly more complex, robust, and less efficient compared to random graphs ( P < 0.05), indicating a hub-independent high-robustness strategy to maintain structural homeostasis. The important roles of specific MEN modules in shaping leaf saponin profiles of each Panax species were proved by multiple machine learning algorithms. Positive regulation modules (PRMs) of total saponin content were further identified, which exhibited more deterministic ecological assembly and comprised of highly connected nodes as well as higher proportion of plant-associated fungal guilds compared to other network members, indicating their tight links with host plant. The significant and direct effects ( P < 0.05) of PRMs on total saponin accumulation were validated by well-fitted structural equation models (χ 2 < 0.3, P > 0.5). Taxonomic analysis revealed that Pleosporales and Chaetothyriales were significantly overrepresented by positive regulation taxa (PRT) of total saponin content (FDR < 0.05). Across PRT identified in three Panax species, Epicoccum and Coniothyrium were conservatively present, while species-specific taxa such as Agaricales were also found, indicating the conservatism and specificity of plant-fungi interactions associated with leaf saponin accumulation in Panax genus. Conclusions These findings provide a foundation for understanding mechanisms maintaining the steady state of phyllosphere mycobiome in healthy plant, and offer clues for engineering phyllosphere mycobiome to improve the accumulation of bioactive secondary metabolites on the basis of network modules.

Phyllosphere

10.1186/s40793-023-00516-7

Cite

Citations (4)

Effect of Optimized Irrigation With Photon‐Induced Photoacoustic Streaming on Smear Layer Removal, Dentin Microhardness, Attachment Morphology, and Survival of the Stem Cells of Apical Papilla

Lasers in Surgery and Medicine (2021)

Lixuan Wu Shan Jiang Huan Ge Zhiyu Cai Xiaojing Huang

This study aimed to evaluate the effect of optimized irrigation with photon-induced photoacoustic streaming (PIPS) activation of different irrigants (distilled water or ethylenediaminetetraacetic acid [EDTA]) on smear layer removal, dentin microhardness, attachment morphology, and survival of stem cells of the apical papilla (SCAP) in an organotypic root canal model.A total of 144 standardized root segments were randomly allocated into 6 groups for irrigation: (i) NaOCl group, (ii) NaOCl + EDTA group, (iii) NaOCl + PIPS (distilled water) group, (iv) NaOCl + PIPS (EDTA) group, (v) NaOCl + EDTA + PIPS (distilled water) group, and (vi) NaOCl + EDTA + PIPS (EDTA) group. Each group was divided into four subgroups for assessment: (i) dentin cleanliness; (ii) dentin microhardness; (iii) cell attachment morphology; and (iv) viable SCAP quantification.Compared with the control groups, the NaOCl + EDTA + PIPS (EDTA) group showed higher efficiency in smear layer removal and in increasing SCAP viability with more stretched cellular morphology. There were no statistically significant differences in either smear layer removal effect, dentin microhardness, attachment morphology, or survival of SCAP among the other groups when optimized with PIPS (distilled water or EDTA) (P > 0.05).Our findings indicated that irrigation optimized with PIPS activation of EDTA for 40 seconds was conducive to smear layer removal without additional dentin microhardness decrease. Additionally, this irrigation created more cell-friendly dentin conditioning than other approaches, which was beneficial for the attachment and survival of SCAP. Lasers Surg. Med. © 2021 Wiley Periodicals LLC.

Smear layer

Distilled water

10.1002/lsm.23394

Cite

Citations (10)

NataMYB4, a flower specific gene, regulates the flavonoid biosynthesis in Chinese Narcissus

Scientia Horticulturae (2023)

Muhammad Anwar Shuhua Duan Mingjun Ma Xuerong Chen Lixuan Wu

MYB

Petal

Flavonoid Biosynthesis

Phenylpropanoid