Jevrosima Stevanović

University of Belgrade

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Zoran Stanimirović

University of Belgrade

Uroš Glavinić

University of Belgrade

Predrag Simeunović

BRAC

Marko Ristanić

University of Belgrade

Nevenka Aleksić

University of Belgrade

Branislav Vejnović

University of Belgrade

Nada Lakić

University of Belgrade

Miloš Vučićević

University of Belgrade

Vladimir Dimitrijević

University of Belgrade

Bojan Gajić

United Arab Emirates University

Cooperative Institutions

University of Belgrade

112

Croatian Veterinary Institute

University of Veterinary Medicine

University of Novi Sad

University of Belgrade – Faculty of Veterinary Medicine

Institut za filozofiju

Scientific Veterinary Institute "Novi Sad"

Institut za Reumatologiju

Institut Sains dan Teknologi Al-Kamal

University of Ljubljana

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Genome-Wide Analysis of Milk Production Traits and Selection Signatures in Serbian Holstein-Friesian Cattle

Animals (2024)

Marko Ristanić Minja Zorc Uroš Glavinić Jevrosima Stevanović Jovan Blagojević

To improve the genomic evaluation of milk-related traits in Holstein-Friesian (HF) cattle it is essential to identify the associated candidate genes. Novel SNP-based analyses, such as the genetic mapping of inherited diseases, GWAS, and genomic selection, have led to a new era of research. The aim of this study was to analyze the association of each individual SNP in Serbian HF cattle with milk production traits and inbreeding levels. The SNP 60 K chip Axiom Bovine BovMDv3 was deployed for the genotyping of 334 HF cows. The obtained genomic results, together with the collected phenotypic data, were used for a GWAS. Moreover, the identification of ROH segments was performed and served for inbreeding coefficient evaluation and ROH island detection. Using a GWAS, a polymorphism, rs110619097 (located in the intron of the CTNNA3 gene), was detected to be significantly (p < 0.01) associated with the milk protein concentration in the first lactation (adjusted to 305 days). The average genomic inbreeding value (FROH) was 0.079. ROH islands were discovered in proximity to genes associated with milk production traits and genomic regions under selection pressure for other economically important traits of dairy cattle. The findings of this pilot study provide useful information for a better understanding of the genetic architecture of milk production traits in Serbian HF dairy cows and can be used to improve lactation performances in Serbian HF cattle breeding programs.

Genome-wide Association Study

SNP genotyping

Runs of Homozygosity

Candidate gene

Bovine genome

SNP

Synteny

10.3390/ani14050669

Cite

Citations (3)

Contact varroacidal efficacy of lithium citrate and its influence on viral loads, immune parameters and oxidative stress of honey bees in a field experiment

Frontiers in Physiology (2022)

Nemanja M. Jovanović Uroš Glavinić Marko Ristanić Branislav Vejnović Jevrosima Stevanović

With an almost global distribution, Varroa destuctor is the leading cause of weakening and loss of honey bee colonies. New substances are constantly being tested in order to find those that will exhibit high anti-Varroa efficacy at low doses/concentrations, without unwanted effects on bees. Lithium (Li) salts stood out as candidates based on previous research. The aims of this study were to evaluate Li citrate hydrate (Li-cit) for its contact efficacy against Varroa, but also the effect of Li-cit on honey bees by estimating loads of honey bee viruses, expression levels of immune-related genes and genes for antioxidative enzymes and oxidative stress parameters on two sampling occasions, before the treatment and after the treatment. Our experiment was performed on four groups, each consisting of seven colonies. Two groups were treated with the test compound, one receiving 5 mM and the other 10 mM of Li-cit; the third received oxalic acid treatment (OA group) and served as positive control, and the fourth was negative control (C group), treated with 50% w/v pure sucrose-water syrup. Single trickling treatment was applied in all groups. Both tested concentrations of Li-cit, 5 and 10 mM, expressed high varroacidal efficacy, 96.85% and 96.80%, respectively. Load of Chronic Bee Paralysis Virus significantly decreased (p < 0.01) after the treatment in group treated with 5 mM of Li-cit. In OA group, loads of Acute Bee Paralysis Virus and Deformed Wing Virus significantly (p < 0.05) increased, and in C group, loads of all viruses significantly (p < 0.01 or p < 0.001) increased. Transcript levels of genes for abaecin, apidaecin, defensin and vitellogenin were significantly higher (p < 0.05-p < 0.001), while all oxidative stress parameters were significantly lower (p < 0.05-p < 0.001) after the treatment in both groups treated with Li-cit. All presented results along with easy application indicate benefits of topical Li-cit treatment and complete the mosaic of evidence on the advantages of this salt in the control of Varroa.

10.3389/fphys.2022.1000944

Cite

Citations (8)

New insights into the origin and the genetic status of the Balkan donkey from Serbia

Animal Genetics (2017)

Ljubodrag Stanišić J. Aleksić Vladimir Dimitrijević Predrag Simeunović Uroš Glavinić

Summary The Balkan donkey ( Equus asinus L.) is commonly regarded as a large‐sized, unselected, unstructured and traditionally managed donkey breed. We assessed the current genetic status of the three largest E. asinus populations in the central Balkans (Serbia) by analysing the variability of nuclear microsatellites and the mitochondrial (mt DNA ) control region of 77 and 49 individuals respectively. We further analysed our mt DNA dataset along with 209 published mt DNA sequences of ancient and modern individuals from 19 European and African populations to provide new insights into the origin and the history of the Balkan donkey. Serbian donkey populations are highly genetically diverse at both the nuclear and mt DNA levels despite severe population decline. Traditional Balkan donkeys in Serbia are rather heterogeneous; we found two groups of individuals with similar phenotypic features, somewhat distinct nuclear backgrounds and different proportions of mt DNA haplotypes belonging to matrilineal Clades 1 and 2. Another group, characterized by larger body size, different coat colour, distinct nuclear gene pool and predominantly Clade 2 haplotypes, was delineated as the Banat donkey breed. The maternal landscape of the large Balkan donkey population is highly heterogeneous and more complex than previously thought. Given the two independent domestication events in donkeys, multiple waves of introductions into the Balkans from Greece are hypothesized. Clade 2 donkeys probably appeared in Greece prior to those belonging to Clade 1, whereas expansion and diversification of Clade 1 donkeys within the Balkans predated that of Clade 2 donkeys.

Equus asinus

10.1111/age.12589

Cite

Citations (13)

Preliminary trials on effects of lithium salts on Varroa destructor, honey and wax matrices

Journal of Apicultural Research (2021)

Zoran Stanimirović Uroš Glavinić Nemanja M. Jovanović Marko Ristanić Dušanka Milojković‐Opsenica

In a cage experiment, lithium chloride (LiCl) and lithium citrate hydrate (Li-cit) were tested for varroacidal efficacy and impact on bees. Treatment with Li-cit (4, 7.5, 10, and 25 mM) resulted in 100% varroacidal efficacy and 100% bee survival. Due to better results in the cage experiment, Li-cit was further tested in field experiments on full-sized free-flying colonies treated three times in 6-day intervals. All the concentrations of Li-cit in the field experiment (5, 10, 15, 20, and 25 mM) expressed high varroacidal efficacy: 93.2–95.5%, significantly (p < 0.01) greater than in the negative and positive (amitraz-treated) controls. Lithium residues in honey from brood chambers were much higher nine months after the last treatment (169.3–1756.0 μg/kg) than seven days post-treatment (19.2–27.8 μg/kg). In honey from honey chambers (eligible for human consumption), the average lithium residues were 26.9 μg/kg and 33.7 μg/kg seven days after the last treatment. In wax combs taken from the brood chamber nine months post-treatment, lithium residues ranged from 410 μg/kg to 2314 µg/kg, without significant differences from the negative control. Lithium residues in wax matrices seven days after the last treatment were in a narrow range of 234.3–300 µg/kg, in wax combs and cappings being significantly lower than in commercial wax foundations. For the first time, Li-cit proved to be effective against Varroa destructor under field conditions.

Propolis

Beeswax

Lithium chloride

Royal Jelly

Destructor

10.1080/00218839.2021.1988277

Cite

Citations (13)

Haplotype identification and detection of mitochondrial DNA heteroplasmy in Varroa destructor mites using ARMS and PCR–RFLP methods

Experimental and Applied Acarology (2016)

Bojan Gajić Jevrosima Stevanović Željko Radulović Zoran Kulišić Branislav Vejnović

Heteroplasmy

Restriction site

10.1007/s10493-016-0086-6

Cite

Citations (19)

Use of Thymol in Nosema ceranae Control and Health Improvement of Infected Honey Bees

Insects (2022)

Uroš Glavinić Jovan Blagojević Marko Ristanić Jevrosima Stevanović Nada Lakić

Nosema ceranae is the most widespread microsporidian species which infects the honey bees of Apis mellifera by causing the weakening of their colonies and a decline in their productive and reproductive capacities. The only registered product for its control is the antibiotic fumagillin; however, in the European Union, there is no formulation registered for use in beekeeping. Thymol (3-hydroxy-p-cymene) is a natural essential-oil ingredient derived from Thymus vulgaris, which has been used in Varroa control for decades. The aim of this study was to investigate the effect of thymol supplementation on the expression of immune-related genes and the parameters of oxidative stress and bee survival, as well as spore loads in bees infected with the microsporidian parasite N. ceranae. The results reveal mostly positive effects of thymol on health (increasing levels of immune-related genes and values of oxidative stress parameters, and decreasing Nosema spore loads) when applied to Nosema-infected bees. Moreover, supplementation with thymol did not induce negative effects in Nosema-infected bees. However, our results indicate that in Nosema-free bees, thymol itself could cause certain disorders (affecting bee survival, decreasing oxidative capacity, and downregulation of some immune-related gene expressions), showing that one should be careful with preventive, uncontrolled, and excessive use of thymol. Thus, further research is needed to reveal the effect of this phytogenic supplement on the immunity of uninfected bees.

Nosema ceranae

Thymol

10.3390/insects13070574

Cite

Citations (28)

Monitoring of mitotic index and frequency of micronuclei in evaluation of genotoxic potential of fumagillin (dicyclohexylamine) in vivo

Acta veterinaria (2006)

Jevrosima Stevanović Zoran Stanimirović Pejin Ivana Miloš Lazarević

Fumagillin (dicyclohexylamine) is a natural antibiotic, secreted by Aspergillus fumigatus. It is used in veterinary medicine against microsporidiosis in bees and fish, as well as in human medicine for the treatment of intestinal amebiasis, microsporidial keratoconjunctivitis and intestinal microsporidiosis due to Enterocytozoon bieneusi in patients with AIDS and other types of immunodeficiency. In this study, the genotoxicity of fumagillin was evaluated in mouse bone marrow cells using the mitotic index (MI) and micronucleus (MN) assay. Fumagillin was administered to BALB/c mice by gavage in doses of 25, 50, 75 mg/kg b.w., repeated for 7 days at 24h intervals, with water-sugar syrup as the negative control and cyclophosphamide as the positive control (40 mg/kg b.w) All experimental doses of fumagillin induced a significant decrease (p<0.001) in MI (3.47 ± 0.04%, 3.17 ± 0.01% and 2.27 ± 0.02%, respectively) in comparison with the negative control (6.00 ± 0.01%) and with the positive control (14.78 ± 0.09). Fumagillin significantly (p<0.001) increased the frequency of MN (4.98 ± 0.35, 8.45 ± 0.57 and 12.02 ± 0.37, respectively) over the negative control (1.04 ± 0.28). These results suggest that fumagillin (dicyclohexilamine) has an antiproliferative and genotoxic potential in mammal in vivo test.

Fumagillin

Mitotic index

10.2298/avb0606437s

Cite

Citations (9)

The influence of pulverised sugar dusting on the degree of infestation of honey bee colonies with Varroa destructor

Acta veterinaria (2011)

Zoran Stanimirović Nevenka Aleksić Jevrosima Stevanović Dragan Ćirković Milorad Mirilović

The aim of this work was the investigation on the efficacy of pulverised sugar dusting on knocking-down Varroa destructor mites and the influence of the dynamics of the treatment on the degree of infestation in honey bee colonies. Two methods were deployed to measure the degree of infestation of bee colonies with V. destructor mites: the sugar shake method and the technique which involves mesh bottom boards equipped with sticky inserts. The research was carried out on 30 strong honey bee colonies in three successive years. The results proved that dusting with pulverised sugar (particle diameter below 40 μm) influenced the fall of V. destructor in comparison with both their fall off prior to the treatment and the negative control. The most discernible effects on the mite fall and the decline in their population in the hives was obtained with dustings repeated at threeday intervals. To conclude, the dynamics of the treatment affected the degree of infestation in bee colonies. However, the efficacy of sugar dusting was significantly lower in comparison with the one of flumethrin (positive control). Thus, in spite of considerable favourable effects on the decline in the degree of infestation with mites, by no means may dusting with pulverised sugar be advised to beekeepers as the one and only means of bee protection against V. destructor. The use of the technique which involves mesh bottom boards equipped with sticky inserts proved more efficacious and sensitive in the judgment of the degree of infestation of bee colonies in comparison with the sugar shake method, which includes dusting 300 bees with 40 g of pulverised sugar (particle diameter below 40 μm).

Destructor

Degree (music)

10.2298/avb1103309s

Cite

Citations (17)

Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae

PLoS ONE (2017)

Uroš Glavinić Biljana Stanković Vladimir Drašković Jevrosima Stevanović Tamaš Petrović

Microsporidium Nosema ceranae is well known for exerting a negative impact on honey bee health, including down-regulation of immunoregulatory genes. Protein nutrition has been proven to have beneficial effects on bee immunity and other aspects of bee health. Bearing this in mind, the aim of our study was to evaluate the potential of a dietary amino acid and vitamin complex "BEEWELL AminoPlus" to protect honey bees from immunosuppression induced by N. ceranae. In a laboratory experiment bees were infected with N. ceranae and treated with supplement on first, third, sixth and ninth day after emergence. The expression of genes for immune-related peptides (abaecin, apidaecin, hymenoptaecin, defensin and vitellogenin) was compared between groups. The results revealed significantly lower (p<0.01 or p<0.001) numbers of Nosema spores in supplemented groups than in the control especially on day 12 post infection. With the exception of abacein, the expression levels of immune-related peptides were significantly suppressed (p<0.01 or p<0.001) in control group on the 12th day post infection, compared to bees that received the supplement. It was supposed that N. ceranae had a negative impact on bee immunity and that the tested amino acid and vitamin complex modified the expression of immune-related genes in honey bees compromised by infection, suggesting immune-stimulation that reflects in the increase in resistance to diseases and reduced bee mortality. The supplement exerted best efficacy when applied simultaneously with Nosema infection, which can help us to assume the most suitable period for its application in the hive.

Nosema ceranae

Immunosuppression

10.1371/journal.pone.0187726

Cite

Citations (94)

Characteristics of Nosema ceranae infection in Serbian honey bee colonies

Apidologie (2013)

Jevrosima Stevanović Predrag Simeunović Bojan Gajić Nada Lakić Dejan Radović

Nosema ceranae

10.1007/s13592-013-0203-z

Cite

Citations (65)