L. I. Korochkin

Isolation and cultivation of stem and progenitor cells of human embryos and fetuses at the age of 7-12 weeks of gestation have been described. The embryonic cells of human brain formed neurospheres with heterogenous composition. Cell differentiation took place not only in the presence of serum or as a result of attachment of neurosphere to a sublayer, but also in floating neurospheres in the presence of mitogens. In most neurospheres, the nestin-immunopositive cells were located near the surface while the cells stained for beta-tubulin III and glial fibrillar acid protein, as compact groups inside the neurospheres.

Neurosphere

Nestin

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Citations (13)

Neural Stem Cells in the Mammalian Brain

International review of cytology (2008)

A. V. Revishchin L. I. Korochkin V. E. Okhotin Г. В. Павлова

10.1016/s0074-7696(07)65002-5

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Citations (11)

IMMUNOHISTOCHEMICAL STUDIES OF S‐100 PROTEIN DURING POSTNATAL ONTOGENESIS OF THE BRAIN OF TWO STRAINS OF RATS

Journal of Neurochemistry (1972)

S. M. Sviridov L. I. Korochkin Volodymyr Ivanov E. I. Maletskaya T. K. Bakhtina

Abstract— We have studied the dynamics of the appearance of cells reacting positively with anti‐S‐100 protein antiserum, during postnatal neurocytogenesis in the brain of rats of two strains differing in their susceptibility to sound stimuli. The postnatal time of appearance of cells reacting positively with anti‐S‐100 protein antiserum was somewhat later in rats susceptible to sound‐induced seizures than in sound‐resistant rats. These differences concerned mainly the cerebral cortex of 12‐day‐old rats. By day 21 of postnatal life these differences had disappeared. In subcortical structures of the brain, S‐100 protein was first found on the 4th to the 5th day of life and the rate of appearance of cells containing this protein was similar in the two strains.

10.1111/j.1471-4159.1972.tb01386.x

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Citations (68)

[Genetic control of the substrate specificity of sheep plasma arylesterase].

PubMed (1975)

В. И. Глазко O. L. Serov L. I. Korochkin

By means of starch gel electrophoresis blood plasma esterases in sheep of different breeds were studied. It is shown that the esterase pattern is a poly-enzyme system consisting of at least three enzymes: arylesterase, carboxylesterase and choline esterase. Postnatal changes of esterase pattern in sheep blood plasma were also studied. Polymorphism on substrate specificities is described, which is expressed in the fact that different arylesterase variants have different affinity to alpha- and beta-isomers of carbone ethers of naphtol. The breeding test suggests that two allelic autosomal genes, reffered to as Es-1a and Es-1b, control the substrate specificity of arylesterase in sheep. The data are discussed in connection with Es-1a and Es-1b gene expression in heterozygous sheep, with the effect of (mosaic) dominance.

Arylesterase

Esterase

Carboxylesterase

Blood plasma

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Citations (3)

Comparison of the structure and embryonic expression ofDelta inDrosophila virilis andD. melanogaster

Molecular Biology (2000)

Наталия Николаевна Рыбцова E. S. Zelentsova George Lezin М. Б. Евгеньев L. I. Korochkin

Drosophila virilis

Melanogaster