Ahmad F. Shahaby

Taif University

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Assessment Mixed Culture of Actinomyces and Sacchromyces for biodegradation of Complex Mineral Oil hydrocarbon

Ahmad F. Shahaby Taif Universit

Hydrocarbon degradation in soil by microorganisms has undergone renewed emphasis because of the increased incidence of petroleum-based pollution. Knowledge about hydrocarbon degradation is needed to determine how microorganisms might be utilized in the removal of the pollutants from the environment. Bioremediation is a modern method in which the natural ability of microorganisms is employed for the reduction of the concentration and/or toxicity of various chemical substances, such as petroleum derivatives, aliphatic and aromatic hydrocarbons, industrial solvents, pesticides and metals. The class ISSN: 2319-7706 Volume 3 Number 4 (2014) pp. 401-414 http://www.ijcmas.com

Mineral oil

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Molecular Characterization of Rhizobiophages Specific for Rhizobium sp. Sinorhizobum sp., and Bradyrhizobium sp.

Ahmad F. Shahaby Abdulla A. Alharthi Adel E. El-Tarras

Rhizobiophages are considered one of the most important biological factors negatively affecting the numbers and activity of rhizobia. They directly lead to lysis of rhizobial cells resulted in reducing their population in soil. In addition, they indirectly affect the ability of rhizobia to fix nitrogen due to the formation of phageresistant strains which have less or no nitrogen fixation capacity. Rhizobiophages were isolated from different sources such as soils, nodules, roots, stems and cultures ISSN: 2319-7706 Volume 3 Number 5 (2014) pp. 155-171 http://www.ijcmas.com

Bradyrhizobium

Bradyrhizobium japonicum

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International Journal of Current Microbiology and Applied Sciences (2016)

Ahmad F. Shahaby Abdulla A. Alharthi Adel E. El Tarras

Taif region is situated in the central foothills of the Sarawat mountains at an altitude of 2200 to 2500 m above the sea level.Special type of pomegranate is cultivated there and called taify pomegranate.Therefore, exploring the types of microorganisms tolerant to root exudates is important.The objective of this study was to identify bacteria, actinomycetes and potential bacterial pathogens of pomegranate roots and their antimicrobial susceptibility patterns as well as effect of fruit parts extract on isolated bacteria.A total of 102 isolate were isolated and identified by morphological, physiological, APi profiles and 16S-rRNA techniques.Out of 102 isolated bacteria, from free soil 38 (37.3%), rhizosphere 55(53.9%)and inside roots 9(8.8%).Isolated Actinomycetes represent 23 (22.5%),Streptomyces21 (20.6%) and Sacchromyces cerevieace 5(4.9%) of all isolated microorganisms.Isolates were identified as B. subtilis, S. cerevieace, S. pneumoniae, S. pyogenes, K. pneumoniae, A. baumannii, P. aerogenosa, A. chroococum, H. seropedicae, A. lipoferumand E.aerogenes.Susceptibility testing was done according to Clinical and Laboratory Standards Institute (CLSI) guideline.Tetracycline was effective against 75.5% of isolated pathogens.In general, Gram positive isolates were more susceptible to all antibiotic tested, whereas Gram negative isolates were less susceptible to all antibiotic tested.Antimicrobial activity of crude extracts of rind, fruit, and juice ofpomegranate fruitswere evaluated against 18Grampositiveand 35 Gram-negative bacteria.Crude alcoholic extracts of rind, seeds extract (juice) and whole fruit were evaluated against isolated Gram-positive and Gram-negative by agar disc diffusion method.All fruit parts were active against all tested bacteria (B.subtilis, E. aerogenes, K. pneumonia and P. aerogenosa), and moreover, against N 2 -Fixing bacteria (A.chroococcum, A.lipoferum and H. seropedicae).Rind extract was less active against P. aeruginosa, juice against E. aerogenes, whole fruit extract against B. subtilis.In general, juice (seed extract) was relatively more active than extracts of other parts of the fruit.The descending order of activity of the extracted parts of the plant fruit was juice, rind and whole fruit, respectively.On the other hand, Gram-positive bacteria, B. subtilis S. pneumoniae and A. chroococcum were more sensitive than Gram-negative and their inhibition zones.Therefore, it may be concluded that fruits of pomegranate extracts have broad spectrum antimicrobial activity and their activity could be attributed, to a great extent, to their contents of alkaloids and flavonoids.

10.20546/ijcmas.2016.502

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Effect of altitude on some male fertility related traits in Saudi ovine and caprine species

Annals of Animal Science (2015)

Nabil S. Awad Mohamed Mohamed Soliman Alaa Mohamed Ayman Sabry Ahmad F. Shahaby

Abstract This study aimed to evaluate the effect of altitude on some male fertility related traits in Saudi sheep and goats. Testicular tissues were collected from a slaughterhouse in Taif governorate (1800 m above sea level) and Makkah governorate (sea level). Sperm characteristics (1 - individual motility, 2 - percent live sperm 3 - percent abnormal sperm) were examined. Semi-quantitative RT -PCR assay was used to evaluate the expression of IGF-II, StAR, LDLr and CYP11A genes. No significant effect of altitude on tested sperm parameters was revealed. Expression of IGF-II gene in both sheep and goats was significantly (P<0.05) higher at sea level compared to high altitude. A similar effect of altitude on StAR gene expression was only observed in goats, while in sheep the level of effect did not reach the significance threshold. Moreover, LDLr gene expression was significantly (P<0.05) higher for both sheep and goats at high altitude than at sea level. The CYP11A gene expression was significantly (P<0.05) higher in high altitude sheep than in those raised at sea level, while the opposite trend was observed for goats. In conclusion, high altitude had an effect on the expression of some studied male fertility related genes, but sperm parameters were not significantly affected.

Male fertility

10.1515/aoas-2015-0007

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Bioremediation of Petroleum Oil by Potential Biosurfactant-Producing Bacteria using Gravimetric Assay

International Journal of Current Microbiology and Applied Sciences (2015)

Ahmad F. Shahaby Abdulla A. Alharthi A. E. El-Tarras

Bioremediation is an efficient strategy for cleaning up sites contaminated with organic pollutants. Oil is worldwide contaminant that can reach toxic concentrations that are detrimental to the environment and human health. The present study investigated bioremediation, by gravimetric analysis, the ability of theoil-degrading bacteria to produce biosurfecants. Four bacterial strains were used in the study. Strains were local isolated strains by enrichment culture technique from polluted sites in industrial area of Taif, Saudi Arabia. These isolates were identified through morphological, biochemical and 16S-rRNA gene sequencing techniques as Bacillus thuringiensis AT5, Actinomyces octodloyts AF104, Pseduomonas aerogenosa AF11-GT and Pseduomonas stutzeri AT3.Their ability to degrade crude oil hydrocarbon were monitored by gravimetric assay for 5 days. The total viable count of isolates ranged from 1.7 x10 4 - 2.1x10 6 CFU mL -1 culture. The four strains showed relatively high capacity and wide spectrum to degrade the crude oil hydrocarbons. Strain AF11-GTshowed high biodegradation efficiency up to 93.41 % when grown on 3, and 5g L -1 oil hydrocarbon by the second day.An increase in oil degradation was correlated to an increase in cell number indicating that the bacterial isolates were responsible for the oil degradation. Potential biosurfactant production methods showed that isolates are biosurfactant producers and the superior isolate was AF11-GT. Our results obtained demonstrate the potential for biodegradation of AF11-GTisolate in situ and/or ex situ as well as in microbial enhancing oil recovery (MEOR) agent. K e y w o r d s Bioremedation, Biosurfacants, Oil-degrading bacteria, Gravimetry.

Pseudomonas stutzeri

Gravimetric analysis

Microbial consortium

Bacillus thuringiensis

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Screening of Natural Bacterial Flora of Pomegranate Roots (Punica granatum L.) and their Antibiotic Activity in Taif, Saudi Arabia

International Journal of Current Microbiology and Applied Sciences (2016)

Ahmad F. Shahaby Abdulla A. Alharthi Adel E. El Tarras

Taif region is situated in the central foothills of the Sarawat mountains at an altitude of 2200 to 2500 m above the sea level. Special type of pomegranate is cultivated there and called taify pomegranate. Therefore, exploring the types of microorganisms tolerant to root exudates is important. The objective of this study was to identify bacteria, actinomycetes and potential bacterial pathogens of pomegranate roots and their antimicrobial susceptibility patterns as well as effect of fruit parts extract on isolated bacteria. A total of 102 isolate were isolated and identified by morphological, physiological, APi profiles and 16S-rRNA techniques. Out of 102 isolated bacteria, from free soil 38 (37.3%), rhizosphere 55(53.9%) and inside roots 9(8.8%). Isolated Actinomycetes represent 23 (22.5%), Streptomyces21 (20.6%) and Sacchromyces cerevieace 5(4.9%) of all isolated microorganisms. Isolates were identified as B. subtilis, S. cerevieace, S. pneumoniae, S. pyogenes, K. pneumoniae, A. baumannii, P. aerogenosa, A. chroococum, H. seropedicae, A. lipoferumand E.aerogenes. Susceptibility testing was done according to Clinical and Laboratory Standards Institute (CLSI) guideline. Tetracycline was effective against 75.5% of isolated pathogens. In general, Gram positive isolates were more susceptible to all antibiotic tested, whereas Gram negative isolates were less susceptible to all antibiotic tested. Antimicrobial activity of crude extracts of rind, fruit, and juice ofpomegranate fruitswere evaluated against 18Gram-positiveand 35 Gram-negative bacteria. Crude alcoholic extracts of rind, seeds extract (juice) and whole fruit were evaluated against isolated Gram-positive and Gram-negative by agar disc diffusion method. All fruit parts were active against all tested bacteria (B. subtilis, E. aerogenes, K. pneumonia and P. aerogenosa), and moreover, against N2-Fixing bacteria (A. chroococcum, A.lipoferum and H. seropedicae). Rind extract was less active against P. aeruginosa, juice against E. aerogenes, whole fruit extract against B. subtilis. In general, juice (seed extract) was relatively more active than extracts of other parts of the fruit. The descending order of activity of the extracted parts of the plant fruit was juice, rind and whole fruit, respectively. On the other hand, Gram- positive bacteria, B. subtilis S. pneumoniae and A. chroococcum were more sensitive than Gram-negative and their inhibition zones. Therefore, it may be concluded that fruits of pomegranate extracts have broad spectrum antimicrobial activity and their activity could be attributed, to a great extent, to their contents of alkaloids and flavonoids.

Punica

Flora

10.20546/ijcmas.2016.502.001

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Citations (4)

Assessment of Genetic Diversity in Saudi Goats, Saudi Arabia Using Genetic Finger printing

Adel E. El-Tarras Ahmad F. Shahaby A Banaja

Goat (Capra aegagrus), as one of the ancient livestock, was first domesticated in Western Asia in the period of 9000 7000BC (Zenuner 1963). It was considered that the domestic goat breeds in the world were from a single origin and their separations in different ecological areas (Nozawa 1988). Zhang and Li (1990) suggested that the goat in the world could be divided into three populations according to the Hb gene frequency (Li et al., 2004). Generally, goat breeds are named by their places of origin and classified by their uses of products. Asthe effect of different ecological conditions and long-term artificial selection, goat breeds with different genetic characteristics and product orientation are formed (Yue 2000).The largest number of goats is observed in Asia, followed by Africa, representing about 59.7% and 33.8%, summing up to 93.5% out of the total number of the world, Dairy goats ISSN: 2319-7706 Volume 4 Number 4 (2015) pp. 223-231 http://www.ijcmas.com

Genetic Resources

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