P. Amaravathi

Sri Venkateswara Veterinary University

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Ch. Srilatha

Professor Jayashankar Telangana State Agricultural University

N. Sailaja

Central University of Odisha

K. Sujatha

Dr. M.G.R. Educational and Research Institute

I. Hemanth

Sri Venkateswara Veterinary University

D. Sreenivasulu

Post Graduate Institute of Medical Education and Research

G. Vani

Sri Venkateswara Veterinary University

P. Veena

Jawaharlal Institute of Post Graduate Medical Education and Research

J Prabhakar

Sri Venkateswara Veterinary University

Ting Rao

Renmin Hospital of Wuhan University

K. Sujatha

Jindal Naturecure Institute

Cooperative Institutions

Sri Venkateswara Veterinary University

P.V. Narsimha Rao Telangana Veterinary University

Dr. NTR University of Health Sciences

ICAR - National Meat Research Institute

Rajiv Gandhi University of Health Sciences

University of Veterinary Medicine

Karnataka Veterinary Animal and Fisheries Sciences University

Saveetha University

Tamil Nadu Agricultural University

Amrita Institute of Medical Sciences and Research Centre

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EFFECT OF LEAD ACETATE ON SPERM MORPHOLOGY AND TESTIS OF WISTAR ALBINO RATS

The Indian Journal of Animal Reproduction (2015)

K. Sujatha Ch. Srilatha Y. Anjaneyulu Ting Rao D. Sreenivasulu

In the present study different doses of lead acetate was used to know the effect on testis, sperm count, sperm morphology in different experimental groups. 108 male wistar rats were divided into three equal groups (I, II &III) i.e. each group contains 36 rats. Group I kept as control. Group II & III rats were orally administered lead acetate @ 60 mgs & 30mgs /kg. bwt / 3 days in a week for 90 days. Every fortnight from each group rats were sacrificed with anesthetic ether and epididymis collected for sperm count, testes for measurement of testicular weight and pieces of testis fixed in Bovin’s fixative for histopathological and histochemical studies. The study revealed that lead acetate caused a dose dependant reduction in sperm count, testicular weight, and abnormal sperm count was increased significantly when compared to control. Hisopathologically testis shows necrosis of seminiferous tubules. Complete separation and disappearance of seminiferous tubular epithelium. Lead also caused reduction in testicular alkaline phosphatase activity.

Lead acetate

Seminiferous tubule

Fixative

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Citations (3)

Histopathological Observations in Rabbits Experimentally Infected with Trypanosoma evansi

DOAJ (DOAJ: Directory of Open Access Journals) (2013)

S. Sivajothi V. C. Rayulu K. Sujatha B. Sudhakara Reddy P. Amaravathi

New Zealand white rabbits (n=6) were challenged with the South Indian local strain of Trypanosoma evansi. Each animal was infected with 5x105 trypanosomes subcutaneously. Animals were daily examined for the development of clinical signs using wet blood-films sampled from the ear veins. Clinically, intermittent pyrexia, undulating parasitaemia, anorexia and emaciation were predominant. Three months post infection, rabbits were sacrificed, detailed postmortem examination was carried out and representative tissue samples were fixed. Gross pathological changes including paleness of visceral organs, gelatinization of fat, congested and oedematous lungs,mucoid enteritis, hepatomegaly and splenomegaly were noticed. Histopathologically, internal organs elucidated clear changes consisted of severe hepatic fatty change, pulmonary congestion with thickened interstitial spaces and emphysema, degeneration of the renal epithelium associated with cystic tubular formation and congested red pulp.

Trypanosoma evansi

Trypanosoma vivax

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Citations (4)

Pattern of lamb mortality and its etiopathology in Rayalaseema region of Andhra Pradesh

Indian Journal of Veterinary Pathology (2019)

Sangubotla Venkata Raghavendra Anil Kumar Banothu P. Amaravathi N. Sasidharbabu N. Sailaja

During the period of study of seven months in 172 dead lambs, the post-mortem lesions revealed pulmonary oedema, various types of pneumonia, infarction of liver; pulpy kidneys, various types of enteritis and enlarged, oedematous and haemorrhagic mesenteric lymph nodes. Histologically, suppurative, interstitial, fibrinous, verminous, aspiration and bronchopneumonia and pulmonary adenomatosis (Jaagsiekte) were observed. Mesenteric lymph nodes revealed various pathologies including congestion, depletion of lymphoid cells, oedema, haemorrhages, fibrous tissue proliferation, reactive hyperplasia and lymphoid proliferation. Kidneys showed glomerular nephritis, haemorrhagic glomerulonephritis, parasitic ova and parasite in cystic fluid. Intestine exhibited catarrhal, verminous, haemorrhagic, diphtheritic, necrotic enteritis. Liver lesions included hepatitis, granulomas, abscess, fibrotic nodules and bacterial colonies in portal triad. The mortality of lambs in this region was more due to multiple system aff ections and due to inflammatory origin majorly followed by non inflammatory origin. Inflammatory origin conditions included inflammation of multiple organs was recorded to be highest. Consulting the veterinarian and adopting prophylactic measures and vaccination against specific infectious diseases would help to overcome lamb mortality in sheep flocks.

10.5958/0973-970x.2019.00037.3

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Malignant form of foot and mouth disease outbreak in sheep and goat with reference to cytological and histopathlogical findings

Indian Journal of Veterinary Pathology (2014)

I. Hemanth P. Amaravathi N. Sasidhar Babu Anil Kumar Banothu N. Sailaja

A total of 8 carcasses of sheep and goat were submitted to the Department of Veterinary Pathology, College of Veterinary Science -Proddatur, Kadapa Dt., Andhra Pradesh, from nearby villages, during a period of approximately 30 days. Detailed necropsy was carried out and representative samples were collected and processed for cytological and histological studies by following the standard routine procedures. In all cases, pneumonic lesions were the consistent finding. In lambs and kids, greyish white necrotic foci on ventricular walls of myocardium were the regular and prominent finding. Touch impression smears from necrosed areas of myocardium revealed infiltration of lymphocytes and lymphoblasts predominantly. Heart revealed myocarditis with infiltrating lymphocytes, lymphoblasts and polymorphs. Certain areas of heart revealed necrotising myocarditis, with cardiac muscles undergoing hyaline degeneration. Based on the gross, cytological and histological findings, it is diagnosed and confirmed serologically as FMD in small ruminants.

Foot (prosody)

10.5958/0973-970x.2014.01143.2

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Citations (1)

The combined effect of Trigonella foenum seeds and Coriandrum sativum leaf extracts in alloxan induced diabetes mellitus wister albino rats

Bioinformation (2019)

Sree Sudha Tanguturi Yella Raghupathi Niranjan Kumar C. Ayyanna Anjaly Mary Varghese P. Amaravathi

Diabetes mellitus is a group of heterogeneous disorders commonly presenting with episodes of hyperglycemia and glucose intolerance, as a result of lack of insulin, ineffective insulin action, and/or both. It is our interest to study the effect of ethanolic extract of Trigonella foenum seeds (fenugreek) and Coriandrum sativum leaves (dhaniya) or its combination in alloxan induced diabetes mellitus wistar albino rats. Rats were randomly separated into six groups where group 1 animals received 2% acacia, group 2 animals received alloxan dose of 150 mg/kg, group 3 animals received glibenclamide dose of 0.5 mg/kg and group 4, 5 and 6 animals received ethanolic extracts of Trigonella foenum seeds, Coriandrum sativum leaves and combination of both extracts at the dose of 100mg/kg for 21 days. Different biochemical parameters such as hepatic and renal biomarkers and histopathology of pancreas were studied. Combination of both extracts showed significant decrease in blood glucose, cholesterol, triglycerides, LDL, VLDL levels, SGOT, SGPT, urea, creatinine and increase in HDL levels and body weight than individual extracts. Thus, we show the antidiabetic activity of poly herbal formulation using biochemical and histo pathological data.

Trigonella

Coriandrum

Alloxan

10.6026/97320630015716

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Citations (11)

PCR analysis of mutton and chicken samples for the presence of Shiga Toxigenic E. coli

Archives of Clinical Microbiology (2011)

Ch. Bindu Kiranmayi N. V. Krishnaiah Subhashini Nelapati P. Amaravathi M. C. Maheswari

Shigatoxigenic Escherichia coli strains are major food-borne pathogens that are transmittedthrough contaminated meat and meat products. Apart from cattle, sheep andgoat are important natural reservoirs. Faecal contamination during slaughter and furtherprocessing along with poor hygienic practices accounts for the presence of STECin raw meat.

Raw meat

Cooked meat

Open Access Publishing

Food contaminant

Meat packing industry

Contaminated food

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Citations (9)

Lead induced thyroid dysfunction in Wistar albino rats and its amelioration with <i>Ocimum sanctum </i>leaf extract and #8211; a hormonal and histopathological study

Journal of Environmental and Occupational Science (2012)

Karamala Sujatha Chinthamaneni Srilatha Tammineedu Rao P. Amaravathi

The objective of this study was to determine the effect chronic administration of different doses of lead acetate and study the role of Ocimum sanctum in lead toxicity as ameliorating agent in wistar rats. In the present investigation rats were randomly divided into six groups (n=36). Rats of group II and III were treated with different doses of lead acetate (60 mgs and 30 mgs / Kg.b.wt. / 3days a week respectively) and Ocimum sanctum orally @ 400mg/Kg b.wt. for other IV and V groups of rats along with lead acetate over a period of 12 weeks and the function of thyroid gland was evaluated by measuring the levels of serum thyroid hormones T3, T4 and TSH and histopathological changes of were studied in thyroid organ at every fortnight interval. Results in the present study revealed that lead acetate caused a dose dependant reduction in T3, T4 and TSH levels when compared to control. Hisopathologically thyroid shows hemorrhages and sever desquamation of epithelial cells, complete absence of acinar colloid in majority of acini, disruption of acini, atrophy of acini and interacinar fibrous tissue proliferation in majority of the animals. In Ocimum treated groups the various thyroid hormonal levels were significantly improved and histopathological changes in thyroid gland was mild when compared to lead treated rats. Based on changes it was concluded that lead acetate at 60 mg/ Kg b.wt. and 30 mg/ Kg b.wt. for 12 weeks was toxic to rats and effect of OS in higher lead dose group was minimum and amelioration was effectively observed in lower dose levels of lead acetate.

10.5455/jeos.20120527015416

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Citations (2)

Efficacy of ivermectin and Tarantula cubensis extract in the treatment of bovine teat papillomatosis

International Journal of Chemical Studies (2020)

MV Swamy Babu P. Veena RV Suresh Kumar P. Amaravathi G. Vani

The objective of this study was to examine the effectiveness of ivermectin and Tarantula cubensis extract in the treatment of teat papillomas in cattle. The present clinical study was conducted on 12 cows with papillomatosis. The animals were divided in to two groups of six animals each and were treated with ivermectin and Tarantula cubensis extract respectively. Three animals in group I and six animals in group II responded well to the treatment. The results of the study revealed that Tarantula cubensis extract was found to be efficacious in the treatment of bovine papillomatosis than ivermectin with no occurrence for a period of 6 months.

Papillomatosis

10.22271/chemi.2020.v8.i3c.9231

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Citations (1)

Clinico-pathological and Immunocytochemical studies on Marek’s Disease in poultry

The Pharma Innovation (2021)

Y Vishnu Prasanth Reddy Amit Kumar P. Amaravathi V Chengalava Rayulu Mvan Suryanarayana

The present investigation was carried out on layer flock belong to Livestock farm complex, College of Veterinary Science, Tirupati. Among 500 birds 41 birds revealed lesions suspected for Marek’s disease and morphologically diagnosed on the basis of gross lesions, cytological smears and immunocytochemistry of different organs. Haematological and biochemical examination revealed significant increase in the lymphocytes count, AST, triglyceride levels and significant decrease in cholesterol levels in the Marek’s disease affected birds when compared to normal birds. Clinically, birds affected with Marek’s disease revealed emaciation, depression with drooping of the wings, anorexia, gasping, reduced weight gain, diarrhoea, pale combs, wattles with presence of nodules on combs, marked reduction in egg production and reduction in size of eggs. Few birds exhibited paralysis with inward curvature of the toes and death of the birds in few days after showing the clinical signs. Grossly, visceral organs (liver, heart, lungs, kidney, ovary, spleen, thymus, pancreas, proventriculus) were enlarged with presence of focal to discrete nodules. Cytological examination of the impression smears collected from the growths of the affected organs during necropsy revealed infiltration of pleomorphic lymphoid cells with thin rim of basophilic cytoplasm. Immunocytochemical staining of the smears revealed moderate to severe immunopositive reactivity for MDV antigens in the cytoplasm of the lymphoid cells.

Emaciation

Marek's disease

White pulp

Proventriculus

Flock

Basophilic

10.22271/tpi.2021.v10.i3i.5836

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Faecal examination and haemato biochemical changes of ailing lambs in Rayalaseema region of Andhra Pradesh

Deleted Journal (2018)

SV Raghavendra Anand Kumar A P. Amaravathi Chengalva Rayulu Anusha

Out of 100 faecal samples collected from ailing lambs, 68 samples were positive for different parasitic ova and oocysts. It was maximum in the age group of 4-6 months. Various parasitic ova identified were eggs of Strongyle, Moniezia sp, Schistosoma sp, Haemonchus sp, Paramphistomum sp, Trichuris sp and oocysts of Coccidia sp. Among 102 blood samples collected from ailing lambs decreased PCV, Hb, TEC, MCHC, neutrophils, monocytes, eosinophils and increased TLC, MCV, MCH, lymphocytes were observed when compared with apparently healthy group (n=10). Out of 50 serum samples from ailing lambs screened, increased TP, albumin, globulin, A/G ratio, creatinine and decreased calcium, ALT were observed when compared with apparently healthy group (n=10).

Coccidia

Trichuris

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Citations (2)