José Jurandir Fagliari

Universidade Estadual Paulista (Unesp)

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André Marcos Santana

Universidade Estadual de Maringá

Thaís Gomes Rocha

Universidade Estadual Paulista (Unesp)

Daniela Gomes da Silva

Universidade Estadual Paulista (Unesp)

Camila Franciosi

Universidade Estadual Paulista (Unesp)

Douglas J. Weiss

University of Vermont

Oral A. Evanson

University of Minnesota

Lucas José Luduverio Pizauro

Universidade Estadual de Santa Cruz

Ricardo Perecin Nociti

Universidade de São Paulo

Kalina Maria de Medeiros Gomes Simplício

Universidade Federal de Sergipe

David McClenahan

University of Northern Iowa

Cooperative Institutions

Universidade Estadual Paulista (Unesp)

201

Universidade de São Paulo

Núcleo de Pesquisas Aplicadas (Brazil)

Universidade Brasil

Hospital Universitário da Universidade de São Paulo

Fundação de Estudo e Pesquisa em Medicina Veterinária e Zootecnia

Faculdade Brasil

Fundação de Apoio à Universidade Federal de São Paulo

Universidade Federal Rural de Pernambuco

Universidade Paulista

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EVALUATION OF PASSIVE IMMUNITY IN NEWBORN GOATS FEEDING WITH GOAT AND COW COLOSTRUM.

Ars Veterinaria (2008)

S. L. Silva José Jurandir Fagliari Paola Fernanda Junqueira Baroza F. T. R. S. Cesco Rodrigo Jorge

Passive immunity

10.15361/2175-0106.2007v23n2p81-88

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Citations (4)

Acute-phase proteins during inflammatory reaction by bacterial infection: Fish-model

Scientific Reports (2019)

Ives Charlie‐Silva André Klein Juliana Moreira Mendonça-Gomes Ed Johnny da Rosa Prado Alessandra Cristina de Moraes

Acute-phase protein (APPs) serum levels have been studied in many human diseases, and their components contribute to host defense during the evolution of infectious diseases by acting as part of the innate immune system. Based on the importance of establishing new experimental models, the present investigation evaluated the modulation of APPs following inflammatory stimulus by the inoculation of Aeromonas hydrophila in tilapias. Fish were sampled 6 and 24 hours post-infection. Tilapias presented increase of positive APPs such as ceruloplasmin, haptoglobin, alpha-2-macroglobulin and complement C3, as well as decrease of negative APPs such as albumin and transferrin. The protein response of tilapias during the course of bacterial infection showed correlation with the kinetics of cellular accumulation in the inflamed focus with significant increase of granulocytes, thrombocytes, lymphocytes and macrophages. However, granulocytes were the predominant cells, associated with increment in the reactive oxygen species (ROS) production. Showing responses similar to those observed in humans, the modulation of APPs and the kinetics of cellular accumulation in the exudate demonstrate the feasibility of this alternative experimental model for advances and studies to understand changes in pathophysiological mechanisms of acute inflammatory reaction due to bacterial infection.

10.1038/s41598-019-41312-z

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ESTUDO DO PROTEINOGRAMA E DOS MINERAIS COBRE, FERRO E ZINCO NO SORO EM OVELHAS DA RAÇA SANTA INÊS COM MASTITE EXPERIMENTAL POR Staphylococcus aureus

DOAJ (DOAJ: Directory of Open Access Journals) (2009)

Nivaldo de Azevêdo Costa Luís Carlos Valeriano Simão Rogério Adriano dos Santos José Augusto Bastos Afonso José Jurandir Fagliari

This study evaluates changes in the serum proteinogram and concentrations of copper, iron, and zinc in Santa Ines primiparous ewes with experimentally induced Staphylococcus aureus mastitis. The right mammary gland of 10 animals were inoculated with 1,0x104 UFC/mL. The clinical exam, poliacrilamide gel eletrophoresis (SDS-PAGE), and determination of Cu, Fe, Zn, and plasma fibrinogen (PF) levels were carried out just before inoculation and after 12, 24, 36, 48, 60, 72, 84, 96, 108, 120, 132, 168, 180, 288, and 336 hours. All animals presented mastitis and subsequent loss of function of the gland. A total of 23 protein fractions were detected. Their molecular weights (MW) varied between 26,000 and 185,000 daltons (Da), which allowed the identification of positive and negative acute phase proteins, immunoglobulins (IgA and IgG), and some nonidentified proteins. A positive correlation between PF and ceruloplasmin (r=0,74), haptoglobin (r=0,62) and IgA (r=0,62) was also evidenced. Results revealed the importance of ceruloplasmin and haptoglobin as acute phase proteins in intramammary infections of ewes and reiterate fibrinogen as an inflammatory marker. Serum alterations of Cu, Fe and Zn indicated inflammatory actions of mediators triggered by S. aureus.

Haptoglobin

Ceruloplasmin

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Intoxicacao natural de bovinos pela micotoxinas esporidesmina . 4 - lesoes hepaticas

Arquivo Brasileiro de Medicina Veterinária e Zootecnia (1994)

Hélio Takashi Okuda A.C. Alessi José Jurandir Fagliari M. Passipieri

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Physiological Concentrations of Acute-Phase Proteins and Immunoglobulins in Equine Synovial Fluid

Journal of Equine Veterinary Science (2012)

Roberta Carvalho Basile Guilherme de Camargo Ferraz Marsel Pereira Carvalho Raquel Mincarelli Albernaz Renatha A. Araújo

Synovial fluid (SF) is capable of reflecting infectious, immunological, or inflammatory joint conditions in horses by altering its composition and appearance. Although plasma and SF compositions are quantitatively different, this latter compartment reflects changes in plasma macromolecules. Therefore, changes in serum immunoglobulin protein concentrations tend also to alter intracapsular levels. Therefore, it is necessary to know the physiological concentrations of proteins present in SF. The aim of this study was to determine the levels of total protein, albumin, transferrin, haptoglobin, α1-acid glycoprotein, ceruloplasmin, and immunoglobulins A and G in SF of six healthy horses. The synovial proteinogram was obtained by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The SF proteins reached a maximum of 25% of serum concentrations, varying inversely with molecular weight of the protein, except for the ceruloplasmin.

Ceruloplasmin

Haptoglobin

Orosomucoid

Sodium dodecyl sulfate

10.1016/j.jevs.2012.05.075

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TEORES SÉRICOS DE PROTEÍNA TOTAL, Ig A E Ig G, OBTIDOS POR ELETROFORESE EM GEL DE POLIACRILAMIDA, EM BEZERROS MESTIÇOS CANCHIM-NELORE, DO NASCIMENTO AOS 30 DIAS DE IDADE

DOAJ (DOAJ: Directory of Open Access Journals) (2009)

Thaís Gomes Rocha Camila Franciosi Ricardo Perecin Nociti Paulo César da Silva Alexandre Amstalden Moraes Sampaio

Serum levels of total protein, immunoglobulin A (IgA), and immunoglobulin G (IgG) – determined by polyacrylamide gel electrophoresis (SDS-PAGE) – were investigated in newborn calves from primiparous and pluriparous cows, before colostrum ingestion and with 1, 2, 7, 15, and 30 days of age. Serum levels of total protein were influenced by the cows’ number of parturitions and were greater in calves from pluriparous cows. Immunoglobulin concentrations revealed a marked increase after the ingestion of colostrum and a decreasing tendency was verified in IgG levels from 24 hours of life to 30 days of age.

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Proteinogramas séricos de ratos Wistar experimentalmente infectados com Trypanosoma evansi

Arquivo Brasileiro de Medicina Veterinária e Zootecnia (2008)

Márcia Cristina Aquino Teixeira Luiz Carlos Marques Fabiano Antônio Cadioli José Jurandir Fagliari Rosângela Zacarias Machado

Estabeleceu-se o perfil eletroforético de proteínas séricas de ratos Wistar experimentalmente infectados com Tripanosoma evansi, utilizando-se 40 ratos, distribuídos em oito grupos de cinco animais cada. Um grupo foi mantido como testemunho (G1), e os demais (G2 a G8) foram inoculados, via intraperitoneal, com cerca de 10³tripomastigota de T. evansi. Amostras de sangue para obtenção de soro foram coletadas no quinto (G2), 10º (G3), 15º (G4), 30º (G5), 45º (G6), 60º (G7) e 75º (G1 e G8) dia após as inoculações. O fracionamento das proteínas foi realizado pela técnica SDS-PAGE. Foram identificadas 31 proteínas, sendo sete de fase aguda: ceruloplasmina (101KD), hemopexina (83KD), transferrina (75KD), albumina (66KD), antitripsina (60KD), haptoglobina (44KD) e glicoproteína ácida (38KD). As proteínas com pesos moleculares 12KD; 22KD; 25KD; 28KD; 32,5KD; 35KD; 53,5KD; 63KD e 72KD apareceram apenas nos ratos inoculados com T. evansi.

Trypanosoma evansi

10.1590/s0102-09352008000600021

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Constituintes sangüíneos de bovinos lactantes, desmamados e adultos das raças Nelore (Bos indicus) e Holandesa (Bos taurus) e de bubalinos (Bubalus bubalis) da raça Murrah

Arquivo Brasileiro de Medicina Veterinária e Zootecnia (1998)

José Jurandir Fagliari A. E. G. Santana Fernando Lucas E. Campos P. R. Curi

Bubalus

Murrah buffalo

Zebu

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Ocorrência de poliúria-polidpsia em eqüinos estabulados - descrição de dois casos de polidipsia psicogênica

Arquivo Brasileiro de Medicina Veterinária e Zootecnia (2000)

José Corrêa de Lacerda Neto Marileda Bonafim Carvalho L. P. Martins Filho Luiz Carlos Marques José Jurandir Fagliari

Dois eqüinos da raça Brasileira de Hipismo, machos, quatro anos de idade, apresentando sinais de poliúria-polidipsia, foram submetidos a completa avaliação clínica e a exames de sangue e urina. Não foram observadas alterações clínicas, hematológicas ou bioquímicas, a não ser um decréscimo na gravidade específica da urina de ambos os animais (1,009 e 1,008). Realizou-se um teste de privação de água de 24 horas e durante esse período a gravidade especifica da urina subiu gradualmente até atingir a normalidade (1,028 e 1,026, respectivamente). O comportamento anormal ocorreu por confinamento excessivo. Os resultados clínicos e de laboratório e o teste de privação de água indicam o diagnóstico de polidipsia psicogênica.

10.1590/s0102-09352000000300007

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Proteinograma sérico de bovinos experimentalmente Infectados com trypanosoma evansi

Ars Veterinaria (2012)

Márcia Cristina Aquino Teixeira Luiz Carlos Marques José Jurandir Fagliari R. Z. Machado P.C. Silva

Trypanosoma evansi is pathogenic to several animal species in tropical and subtropical areas. In Brazil, the disease is endemic in Pantanal mato-grossense. This study aimed to determine the electrophoretic profile of serum proteins in experimentally infected cattle. Eight crossbred animals, aged approximately eight months, clinically healthy and seronegative (IFAT) for Tripanosoma evansi were used. The animals were divided into two groups, G1: five animals inoculated intravenously with 22 x 106 trypomastigotes of T. evansi, and G2: three animals kept as controls. The blood used to obtain serum samples was collected daily until the 15th day after inoculation (DAI), and subsequently, at weekly intervals until the 204th DAI, every 14 days until the 372nd DAI and every 30 days until the 522nd DAI. Electrophoresis in polyacrylamide gel containing sodium dodecyl sulfate (SDS-PAGE) was used for the purification of proteins. Through the biological test, T. evansi was detected in animals 01, 06 and 08 on the 15th DAI, 06 and 07 on the 30th DAI, 01 and 06 on the 45th DAI, 06 on the 60th DAI and 01 on the 75th DAI. Twenty-six proteins with molecular weights ranging from 20 kDa to 245 kDa were found in cattle. Eight of them were nominally identified as immunoglobulin A (IgA), ceruloplasmin, transferrin, albumin, heavy and light chain immunoglobulin G (IgG), haptoglobin and acid glycoprotein.

Trypanosoma evansi

Haptoglobin

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