Although iron is abundant in the earth crust, iron bioavailability to plants is low because of its poor solubility in water. Iron is essential for human health, but iron malnutrition occurs all around the world, therefore, how to fortify crop food of iron is important for maintenance of human health. Beneficial microorganisms, including mycorrhizal and root endophytic fungi, dark septate fungi, and plant growth-promoting rhizobacteria, possess multiple functions, especially those involved in resistance to abiotic and biotic stress and increased mineral uptake. Their efficiency is long-termed once they are applied in crop field. All the beneficial microorganisms can secret siderophores to enhance iron uptake and transport it to their host plants, therefore, application in food crops is a good access to iron biofortification. In the present article, we introduced traits of main beneficial microorganisms and application in crop food. Since some beneficial microorganisms might compete for nutrition each other in field, their synergism should be investigated before they are used in biofilmed biofertilizers specific to some food crops. Application of biofilmed biofertilizers is important for sustainable agriculture and human health.
Selenium (Se) is essential for human health, however, Se is deficient in soil in many places all around the world, resulting in human diseases, such as notorious Keshan disease and Keshin–Beck disease. Therefore, Se biofortification is a popular approach to improve Se uptake and maintain human health. Beneficial microorganisms, including mycorrhizal and root endophytic fungi, dark septate fungi, and plant growth-promoting rhizobacteria (PGPRs), show multiple functions, especially increased plant nutrition uptake, growth and yield, and resistance to abiotic stresses. Such functions can be used for Se biofortification and increased growth and yield under drought and salt stress. The present review summarizes the use of mycorrhizal fungi and PGPRs in Se biofortification, aiming to improving their practical use.