The genetically modified (GM) common bean Embrapa 5.1 was recently approved for commercialization. The reliable detection and quantification of GM organisms is strongly dependent on validated methods as well as calibration systems. This work presents the development of a calibrant plasmid for Embrapa 5.1 common bean detection. The reaction parameters were determined and compared for both the plasmid DNA (pDNA) and the genomic DNA (gDNA). PCR efficiencies for pDNA were 81% for the construction-specific assays and 76% for the taxon-specific assay, whereas for gDNA efficiencies were 94 and 93%, respectively. The limits of detection (LOD) in both qPCR assays were 102 and 103 copies of gDNA and pDNA per PCR reaction, respectively. This is sufficient to detect 0.067 and 0.67% of GM common bean in 100 ng of DNA, respectively, which is in agreement with detecting the 1% GM content required by the Brazilian legislation.

genomic DNA

10.1021/jf400928k

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Intensidade de brusone nas folhas em fases avançadas do desenvolvimento do arroz, baseada no número inicial de lesões

Pesquisa Agropecuária Brasileira (1980)

Josías C. Faria A. S. Prabhu

A intensidade de brusone ( Pyricularia oryzae Cav.) nas folhas, na fase do primordio floral, foi estimada com antecedencia de mais ou menos 40 dias, considerando-se o aumento de doenca como funcao de tempo e inoculo inicial. Foram analisados os dados de cinco curvas de progresso da doenca, em condicoes de campo, em tres cultivares (‘IAC 1246', 'IAC 47', 'Montanha Liso'), durante o periodo 1975/77. A percentagem de area foliar atacada por brusone, no fim da fase logaritmica, foi diretamente proporcional ao inoculo inicial, medido como numero de lesoes por 100 folhas. As equacoes de regressao linear foram desenvolvidas para cinco niveis de inoculo inicial, usando-se a percentagem de area foliar afetada com brusone em datas diferentes, durante a segunda fase da curva do progresso da doenca, na qual a brusone nao excedeu a 35%. As diferencas entre a percentagem estimada de area foliar afetada com brusone, obtida pelas equacoes, e as observadas nas condicoes de campo, na fase de primordio floral, foram menores do que 5%, e na fase de emborrachamento, foram cerca de 10%. A brusone estimada para os niveis de inoculos iniciais, variando de oito a 34 lesoes/100 folhas, foi, aproximadamente, 10%, enquanto que no nivel de 278 lesoes/100 folhas, a intensidade de brusone aumentou para 29%. Estes resultados indicaram a aplicabilidade do uso do metodo estudado para a previsao de brusone atraves do desenvolvimento de equacoes gerais para diversos niveis de inoculos iniciais.

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High incidence of mixed DNA and RNA virus infections in common bean in Central Brazil.

Bruna Pinheiro de Lima D. M. T. Alves-Freitas Márcio Tadeu Godinho Josías C. Faria Cristiano Lacorte

During the winter crop season in 2016 a very high incidence of viruslike symptoms of mosaic, leaf curling and deformation, and plant dwarfing was reported by farmers in central areas of Brazil. Bean plants were collected in commercial farms in Luziânia, Cristalina and experimental plots in Goiânia and Brasilia.

Dwarfing

Growing season

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Inheritance and genetic mapping of the first CPMMV tolerance locus in common bean

Plant Pathology (2024)

Rodrigo S. Silva Josías C. Faria Rosana Pereira Vianello Paula Arielle Mendes Ribeiro Valdisser H. S. Pereira

Abstract Although cowpea mild mottle virus (CPMMV) has been reported in Brazil since 1983, it has only become a significant concern for researchers and farmers in recent years. The objective of this work was to investigate the genetic basis of CPMMV tolerance, mapping and reporting the first loci associated with this trait in common bean (cv. BRS Sublime). Phenotypic assays were carried out on 180 individual plants (F 2 generation) and 180 F 2:3 progenies comprising 12 plants per family and their parents (BRS Sublime, tolerant parent × CNFCT 16207, susceptible parent). CPMMV was mechanically inoculated and symptoms were evaluated at 35 days after inoculation, using a 1–5 scoring scale. A linkage map was constructed using 1695 single‐nucleotide polymorphism (SNP) and SilicoDArT markers that segregated in the F 2 and F 2:3 generations as expected. Markers were distributed across 11 common bean chromosomes, resulting in a total length of 2864 cM, with an average distance between markers of 1.8 cM. Phenotypic observations revealed that tolerance in cv. BRS Sublime is controlled by a single dominant gene. The main effect quantitative trait locus (QTL; CPMMV.Pv08) associated with CPMMV tolerance was identified in the terminal region on chromosome 8 (Pv08). This QTL explained approximately 77% of phenotypic variation, indicating that the inheritance of tolerance to CPMMV is monogenic, controlled by a major locus. As far as we know, this study represents the first investigation into the inheritance and genetic mapping of CPMMV tolerance in common bean, with potential for the development of elite lines with multiple virus resistance/tolerance.

Inheritance

10.1111/ppa.13892

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BRS 7762 Supremo - A black common bean cultivar with erect plant type

Crop Breeding and Applied Biotechnology (2006)

J. G. C. da Costa L. C. de Faria C.A. Rava M. J. Del Peloso L. C. Melo

virus and to four pathotypes caused by the anthracnose agent.

10.12702/1984-7033.v06n02a12

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Resposta de arroz de sequeiro à aplicação de fungicidas

Pesquisa Agropecuária Brasileira (1983)

A. S. Prabhu Josías C. Faria A. J. de Conto

Foi estudada a resposta do arroz de sequeiro ( Oryza sativa L.) a aplicacao de fungicidas, em tres experimentos de campo. Uma pulverizacao de todos os fungicidas testados (benomil, blasticidin-S, edifenphos, kasugamicina, benomil + maneb, kasugamicina + captafol) aumentou a producao em comparacao a testemunha. Nao houve diferenca significativa no controle da brusone do pescoco da panicula nem na producao de graos, entre a aplicacao de benomile kasugasnicina isolados ou misturados com maneb e captafol, respectivamente. Os indices de brusone nas folhas e nas paniculas foram significativamente reduzidos com uma aplicacao de fungicida na epoca da emissao da panicula ou florescimento, ou com duas pulverizacoes em combinacao com qualquer dos dois estadios. Entre os produtos testados para controle de brusone, edifenphos, benomil e benomil + maneb apresentaram resultados superiores na reducao de severidade da mancha-estreita. A analise economica da produtividade alcancada revelou que, no caso de areas propensas a brusone, a alternativa mais viavel e a aplicacao de fungicidas simples.

Maneb

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