Oliver Tills

University of Plymouth

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John I. Spicer

University of Plymouth

Simon D. Rundle

University of Plymouth

Manuela Truebano

University of Plymouth

Ziad Ibbini

University of Plymouth

Jamie C. S. McCoy

University of Plymouth

Markus Pfenninger

Senckenberg Biodiversity and Climate Research Centre

Michael Collins

University of Plymouth

Tabitha S. Rudin-Bitterli

University of Western Australia

Enrico L. Rezende

Anhanguera-Uniderp University

Luke Holmes

University of Plymouth

Cooperative Institutions

University of Plymouth

Goethe University Frankfurt

Senckenberg Biodiversity and Climate Research Centre

Senckenberg Society for Nature Research

LOEWE Centre for Translational Biodiversity Genomics

Senckenberg Research Institute and Natural History Museum Frankfurt/M

University of Exeter

University of Sheffield

British Antarctic Survey

Natural Environment Research Council

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Optical flow motion analysis dataset for Radix balthica multiple environmental driver early developmental stage assay

Zenodo (CERN European Organization for Nuclear Research) (2021)

Oliver Tills I Ziad

Frame-to-frame optical flow motion analysis dataset for Radix balthica trochophore embryos exposed to different combinations of temperature and salinity. Twenty five time points for each embryo are recorded with temperatures separated into individual .h5 result files. Original detail of the wet-lab study from which the video was taken can be found in the third experiment featured in this paper: https://doi.org/10.1371/journal.pbio.3000074. The motion analysis method used was a derivative of the method developed and validated in this paper: https://doi.org/10.1186/1471-2105-14-37.

Radix (gastropod)

Optical Flow

10.5281/zenodo.4582214

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Both maternal and embryonic exposure to mild hypoxia influence embryonic development of the intertidal gastropod Littorina littorea (Linnaeus, 1758)

Journal of Experimental Biology (2020)

Jamie C. S. McCoy John I. Spicer Oliver Tills Simon D. Rundle

There is growing evidence that maternal exposure to environmental stressors can alter offspring phenotype and increase fitness. Here, we investigate the relative and combined effects of maternal and developmental exposure to mild hypoxia (65% and 74% air saturation respectively) on the growth and development of embryos of the marine gastropod Littorina littorea. Differences in embryo morphological traits were driven by the developmental environment, whereas the maternal environment and interactive effects of maternal and developmental environment were the main driver of differences in the timing of developmental events. While developmental exposure to mild hypoxia significantly increased the area of an important respiratory organ, the velum, it significantly delayed hatching of veliger larvae and reduced their size at hatching and overall survival. Maternal exposure had a significant effect on these traits, and interacted with developmental exposure to influence the time of appearance of morphological characters, suggesting that both are important in affecting developmental trajectories. A comparison between embryos that successfully hatched and those that died in mild hypoxia revealed that survivors exhibited hypertrophy in the velum and associated pre-oral cilia suggesting these traits are linked with survival in low oxygen environments. We conclude that both maternal and developmental environments shape offspring phenotype in a species with a complex, developmental life history, and that plasticity in embryo morphology arising from exposure to even small reductions in oxygen tensions impacts the hatching success of these embryos.

Developmental plasticity

Veliger

Hypoxia

Maternal effect

Operculum (bryozoa)

10.1242/jeb.221895

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A phenomics approach reveals interspecific differences in integrated developmental responses to chronic elevated temperatures

Zenodo (CERN European Organization for Nuclear Research) (2023)

Jamie C. S. McCoy John I. Spicer Simon D. Rundle Oliver Tills

Raw data for publication 'A phenomics approach reveals interspecific differences in integrated developmental responses to chronic elevated temperatures', Journal of Experimental Biology (2023) 226, jeb245612. doi:10.1242/jeb.245612. Developmental_Event_Timings.csv - Raw data for the timings of development of Lymnaea stagnalis, Radix balthica and Physella acuta maintained at 20 and 25C, recorded by manual observation from video of developing embryos. Data are recorded as absolute timings, and relative timings normalised between the 4-cell stage and hatching. EPT_Data.csv - Time series of frequency specific EPT data binned at 0.1Hz increments for embryos of Lymnaea stagnalis, Radix balthica and Physella acuta maintained at 20 and 25C, normalised by relative developmental time. EPT_data_by_physiological_window.csv - Frequency specific EPT data binned at 0.1Hz increments, and averaged across 4 key physiological windows in development (ciliary driven rotation, muscular crawling, cardiovascular function and radula function) for embryos of Lymnaea stagnalis, Radix balthica and Physella acuta maintained at 20 and 25C. Total_energy_data.csv - Time series of total energy data for embryos of Lymnaea stagnalis, Radix balthica and Physella acuta maintained at 20 and 25C, normalised by relative developmental time.

Phenomics

10.5281/zenodo.8060340

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De novo transcriptome assembly of the amphipod Gammarus chevreuxi exposed to chronic hypoxia

Marine Genomics (2017)

Michael Collins Oliver Tills John I. Spicer Manuela Truebano

Hypoxia

Gammarus

10.1016/j.margen.2017.01.006

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Citations (14)

Optical flow motion analysis dataset for Radix balthica multiple environmental driver early developmental stage assay

Zenodo (CERN European Organization for Nuclear Research) (2021)

Oliver Tills I Ziad

Radix (gastropod)

Optical Flow

10.5281/zenodo.4582213

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Response Of Different Embryonic Developmental Stages Of Radix Balthica To A 24 H 36°C Exposure

Zenodo (CERN European Organization for Nuclear Research) (2018)

Oliver Tills

Sample datasets and video acquired using the EmbryoPhenomics platform. Experiment assessing influence of culture at 36C for 24 h on three developmental stages (E3, E7 and E9) of the aquatic gastropod Radix balthica. The EmbryoCV package was used to produce phenome-level data. Datasets and associated video for an individual embryo from each developmental stage.

Radix (gastropod)

10.5281/zenodo.1419226

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LabEmbryoCam: An opensource phenotyping system for developing aquatic animals

bioRxiv (Cold Spring Harbor Laboratory) (2023)

Ziad Ibbini Maria Bruning Sakina Allili Luke Holmes Ellen Tully

Abstract Phenomics is the acquisition of high-dimensional data on an individual-wide scale and is proving transformational in areas of biological research related to human health including medicine and the crop sciences. However, more broadly, a lack of available transferrable technologies and research approaches is significantly hindering the uptake of phenomics, in contrast to molecular-omics for which transferrable technologies have been a significant enabler. Aquatic embryos are natural models for phenomics, due to their small size, taxonomic diversity, ecological relevance, and high levels of temporal, spatial and functional change. Here, we present LabEmbryoCam, an autonomous phenotyping platform for timelapse imaging of developing aquatic embryos cultured in a multiwell plate format. The LabEmbryoCam capitalises on 3D printing, single board computers, consumer electronics and stepper motor enabled motion. These provide autonomous X, Y and Z motion, a web application streamlined for rapid setup of experiments, user email notifications and a humidification chamber to reduce evaporation over prolonged acquisitions. Downstream analyses are provided, enabling automated embryo segmentation, heartbeat detection, motion tracking, and energy proxy trait (EPT) measurement. LabEmbryoCam is a scalable, and flexible laboratory instrument, that leverages embryos and early life stages to tackle key global challenges including biological sensitivity assessment, toxicological screening and broader engagement with the earliest stages of life. Specifications table

Phenomics

Traceability

Microstimulation

10.1101/2023.04.11.536373

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Moderate reductions in dissolved oxygen may compromise performance in an ecologically-important estuarine invertebrate

The Science of The Total Environment (2019)

Michael Collins Oliver Tills Lucy M. Turner Melody S. Clark John I. Spicer

Hypoxia

10.1016/j.scitotenv.2019.07.250

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Citations (14)

Response Of E3 Stage Radix Balthica Embryos Maintained In Contrasting Temperatures And Salinities For 24H

Zenodo (CERN European Organization for Nuclear Research) (2018)

Oliver Tills

Sample datasets and video acquired using the EmbryoPhenomics platform. Experiment assessing influence of three (20, 25 and 30C) contrasting temperatures and two salinities (0 or 7 ppt) on the development of the aquatic gastropod Radix balthica. Exposure was initiated at the E3 developmental stage at which embryos are undergoing ciliary driven spinning. The EmbryoCV package was used to produce phenome-level data. Datasets and associated video for an individual embryo from each treatment (temperature and salinity combinations).

Radix (gastropod)

10.5281/zenodo.1419207

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Reduced pH affects pulsing behaviour and body size in ephyrae of the moon jellyfish, Aurelia aurita

Journal of Experimental Marine Biology and Ecology (2016)

Oliver Tills Xiaoming Sun SD Rundle T. Heimbach T.E. Gibson

Aurelia aurita

Scyphozoa

10.1016/j.jembe.2016.03.014

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Citations (19)