Maolian Wei

Shandong Institute for Product Quality Inspection

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Guozhong Chen

Hangzhou Normal University

Linlin Jiang

Ludong University

Xin Yu

Yantai Academy of Agricultural Sciences

Jianlong Zhang

Ludong University

Hongwei Zhu

Tsinghua University

Youzhi Li

Hunan Agricultural University

Wenli Tang

Southern Medical University

Weibo Zheng

Ludong University

Xingxiao Zhang

Ludong University

Yixuan Li

China Agricultural University

Cooperative Institutions

Ludong University

China National Center for Food Safety Risk Assessment

Yantai Academy of Agricultural Sciences

Shandong Agricultural University

Ministry of Agriculture and Rural Affairs

Shandong University

Shandong Institute for Product Quality Inspection

Southern Medical University

Chinese Academy of Sciences

Yantai University

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Spatial and Temporal Persistence of Fluorescent Lactiplantibacillus plantarum RS-09 in Intestinal Tract

Frontiers in Microbiology (2022)

Xiaoyu Zhao Chenpei Zhao Leining Yang Linlin Jiang Jianlong Zhang

The beneficial effects of the probiotic strain Lactiplantibacillus plantarum (formerly Lactobacillus plantarum) are based on its adherence and colonization ability in the gut. However, little is known about the migration and long-term gut colonization of the strain. This study evaluated the gut colonization modes of Lactiplantibacillus plantarum RS-09 to identify the strain with long-term gut colonization potential. We established CFDA/SE-labeled RS-09 to study the temporal and spatial distribution of RS-09 in the intestine as well as to analyze its persistence in different parts of the intestine by flow cytometry. This study has shown that the RS-09 strain maintains strong adhesion abilities under acid (pH 2.5) and base (pH 8.5) conditions. In addition, CFDA/SE can be used as an indicator for the labeling of L. plantarum RS-09 in the intestinal tract in vivo. We established a growth kinetics model of RS-09 to elucidate its persistence in the intestine. In vivo persistence experiments showed that the persistence rate of RS-09 was the highest in the cecum (69.5%) and the lowest in the duodenum (12.8%) at 96 h. After 20 days, RS-09 was predominantly localized in the cecum and colon steadily. These studies provide new insights into the long-term persistence of L. plantarum in the gastrointestinal tract. The CFDA/SE label system may be used to study the in vivo colonization dynamics of other probiotic strains.

Cecum

Persistence (discontinuity)

Strain (injury)

Large intestine

10.3389/fmicb.2022.843650

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Whole genome analysis of a novel adenovirus discovered from Oriolus chinesis

Virus Research (2022)

Weibo Zheng Yixuan Li Wenli Tang Maolian Wei Youzhi Li

Adenovirus genome

Horizontal Gene Transfer

Eukaryote

Comparative Genomics

10.1016/j.virusres.2022.198799

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Citations (3)

Effects of Dietary Manganese Supplemental Level on Serum Biochemical Indexes, Metatarsal Bone Contents and Apparent Digestibility of Manganese and Iron of Weaned Pigs

Dongwu Yingyang Xuebao (2015)

Maolian Wei Yang Weiren Guiguo Zhang Jiang Shuzhen Guo Wenwen

Dietary iron

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Lactobacillus plantarum RS-09 Induces M1-Type Macrophage Immunity Against Salmonella Typhimurium Challenge via the TLR2/NF-κB Signalling Pathway

Frontiers in Pharmacology (2022)

Chenpei Zhao Huan Chen Hao Liang Xiaoyu Zhao Wenli Tang

Lactobacillus plantarum can interact with macrophages against bacterial enteropathy due to its potential ability to modulate macrophage polarization. However, this mechanism is not completely understood. TLR2 can recognize microbial components and trigger macrophage cytokine responses to different gram-positive strains. The aim of this study was to investigate whether probiotic Lactobacillus plantarum RS-09 can induce macrophage polarization against Salmonella Typhimurium infection via TLR2 signalling. BALB/c mice were preadministered RS-09 continuously for 7 days and then infected with Salmonella Typhimurium ATCC14028. Mouse RAW264.7 mononuclear macrophages were stimulated with RS-09 and coincubated with ATCC14028 or PBS controls. The results of the in vivo study indicated that RS-09 could relieve S. Typhimurium-induced splenomegaly, body weight loss and death rate. RS-09 also limited the colonization and translocation of S. Typhimurium in the gastrointestinal tract and thereby protected against infection. We also observed that RS-09 upregulated the production of M1 macrophage characteristics (e.g., CD11c and IL-6) against S. Typhimurium. Furthermore, RS-09 induced the expression of TLR2 in macrophages. In an in vitro study, treatment of RAW264.7 cells with RS-09 either concurrently with or before S. Typhimurium challenge enhanced the secretion of Reactive oxygen species and Nitric oxide. This effect was related to TLR2 and NF-κB activation. Based on these findings, Lactobacillus plantarum RS-09 was shown to modulate M1 macrophage polarization and induce TLR2-linked NF-κB signalling activity in the innate immune response to S. Typhimurium infection.

Macrophage polarization

Salmonella enterica

10.3389/fphar.2022.832245

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Effects of dietary star anise (Illicium verum Hook f) supplementation during gestation and lactation on the performance of lactating multiparous sows and nursing piglets

Animal Science Journal (2014)

Gongying Wang Chongwu Yang Zaibin Yang Weiren Yang Shuzhen Jiang

Abstract Thirty‐two sows were allocated to four treatments to evaluate the effect of dietary star anise ( SA ) supplementation during gestation and lactation on the lactational performance of sows. At 85 days of gestation, sows were randomly allotted to one of two diets supplemented with 0.5% SA or basal diet. After farrowing, sows were further allotted to one of two lactation diets supplemented with 0.5% SA or basal diet. On a weekly basis, body weight ( BW ) of sows and piglets was measured. Blood and milk samples were obtained from the sows and piglets. Number of days from weaning to estrus, milk yield and feed intake were also recorded. Weight gain of piglets from sows fed the SA ‐supplemented diet during lactation was greater between days 7 and 14, days 14 and 21 and the overall experimental period compared with control groups. Supplementation of SA during lactation improved weaning weight of piglets, milk yield and average daily feed intake ( ADFI ) of sows. The SA diet increased concentrations of insulin‐like growth factor‐1 ( IGF ‐1) in ordinary milk and prolactin ( PRL ) in serum of sows. In conclusion, this study has indicated the beneficial effects of dietary SA addition in improving the lactation performance of sows.

10.1111/asj.12300

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Citations (9)

Aerosol Concentrations and Fungal Communities Within Broiler Houses in Different Broiler Growth Stages in Summer

Frontiers in Veterinary Science (2021)

Guozhong Chen Di Ma Qingrong Huang Wenli Tang Maolian Wei

Fungal aerosols in broiler houses are important factors that can harm the health of human beings and broiler. To determine the composite characteristics and changes in fungal aerosols in broiler houses during different broiler growth stages in summer. We analyzed the species, concentration and particle diameter distribution characteristics of the aerosols in poultry houses using an Andersen sampler and internal transcribed spacer 1 (ITS1) high-throughput sequencing technology. The concentration of fungal aerosols in the poultry houses increased as the ages of the broiler increased, which was also accompanied by gradual increases in the variety and diversity indices of the fungal communities in the air of the poultry houses. During the entire broiler growth period, the dominant genera in the fungal aerosols in the poultry houses included Trichosporon, Candida, Aspergillus, Cladosporium and Alternaria . These fungi may be harmful to the health of poultry and human beings, so permanent monitoring of microbial air quality in chicken houses is necessary.

10.3389/fvets.2021.775502

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Citations (10)

Whole Genome Analysis of a Novel Adenovirus Isolated from Oriolus Chinesis

SSRN Electronic Journal (2022)

Weibo Zheng Yixuan Li Wenli Tang Maolian Wei Youzhi Li

We present the first complete genome sequence of an aviadenovirus Oriolus adenovirus (OrAdV) isolated from the cloaca of a Oriolus chinensis (a passerine bird widely distributed in Asia), which was collected from an island off the east coast of China. Twenty-nine protein coding genes were predicted in this 40425-bp-long genome. OrAdV genome is highly divergent and has only 67% average protein identity compared with other adenovirus genomes and all reported proteins. Comparative genomic analysis indicates that this passerine virus is a new species of aviadenovirus. In this genome, 33 KDa protein coding gene is absent. 22 KDa is much shorter than that of other aviadenoviruses and has in-frame stop codons. One unique thymidylate kinase gene was discovered in OrAdV genome. This gene is absent in other adenovirus genomes and usually reported to occur in herpesvirus. Protein sequence alignment against all known proteins indicates that this gene may be originated from ancient horizontal gene transfer event between virus and parasitic eukaryote like protozoan. This new aviadenovirus genome enriches the genomic information of adenovirus and suggests that there is a large unknown space of adenovirus world.

Adenovirus genome

10.2139/ssrn.4043623

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