【Objective】To explore the influence of polymorphism of thymidylate synthetase on the clinical effect of 5-fluorouracil. 【Methods】The genotypes of thymidylate synthetase in 50 untreated esophageal cancer patients were analyzed. The homozygote with twice 28-bp tandem repeated sequence in promoter area was named as S/S,triple 28-bp tandem repeated sequence as R/R,the heterozygote with twice tandem repeated and triple tandem re-peated sequence as R/S. The mRNA level of thymidylate synthetase was detected by RT-PCR. The relationship be-tween the genotype of thymidylate synthetase and clinical effect of 5-fluorouracil was analyzed. 【Results】Among the 50 patients,there were 20 patients of R/R with 5-fluorouracil effect rate of 10%,22 patients of R/S with 5-fluo-rouracil effect rate of 13.6%,8 patients of S/S with 5-fluorouracil effect rate of 50%,the differences of the effect rate among the genotypes of R/R with S/S and R/S with S/S were significant (P 0.05). The mean survival time of the pa-tients of S/S was 20.3 months,which was significantly different from 8.1 months of R/S,and 7.9 months of S/S (P0.05). 【Conclusion】The polymorphism of thymidylate synthetase is a potential factor that may influence the effect of 5-fluorouracil.
Objective: To establish the symptoms and the quantitative assessment rating scale of knee osteoarthritis patients and observe the clinical effect of massage manipulation on the patients,and make the application assessment of the massage manipulation.Methods: Self-made symptoms and functional assessment rating scales were used to observe and compare the clinical effect of 40 knee osteoarthritis patients before and after treatment.Results: The scores of these quantitative rating scales showed the clinical effect scores of the patients after the treatment were lower than that before the treatment(P0.01),indicating that the patients significantly improved.Conclusion: The self-made symptoms and the functional assessment rating scales of the knee osteoarthritis make the basic consistent assessment of the massage manipulation on the treatment of the knee osteoarthritis patients,which illustrates the clinical effect of the massage manipulation on the improvement of the knee osteoarthritis and shows these scales can make the objective assessment of the patient's clinical conditions,and provides a feasible way for the establishment of the quantitative functional assessment rating scales.
