This paper presents pioneering results on the evaluation of noble metal film hetero-structures to improve some functional characteristics of carbon-based implant materials: carbon-composite material (CCM) and carbon-fiber-reinforced polyetheretherketone (CFR-PEEK). Metal-organic chemical vapor deposition (MOCVD) was successfully applied to the deposition of Ir, Pt, and PtIr films on these carriers. A noble metal layer as thin as 1 µm provided clear X-ray imaging of 1−2.5 mm thick CFR-PEEK samples. The coated and pristine CCM and CFR-PEEK samples were further surface-modified with Au and Ag nanoparticles (NPs) through MOCVD and physical vapor deposition (PVD) processes, respectively. The composition and microstructural features, the NPs sizes, and surface concentrations were determined. In vitro biological studies included tests for cytotoxicity and antibacterial properties. A series of samples were selected for subcutaneous implantation in rats (up to 3 months) and histological studies. The bimetallic PtIr-based heterostructures showed no cytotoxicity in vitro, but were less biocompatible due to a dense two-layered fibrous capsule. AuNP heterostructures on CFR-PEEK promoted cell proliferation in vitro and exhibited a strong inhibition of bacterial growth (p < 0.05) and high in vitro biocompatibility, especially Au/Ir structures. AgNP heterostructures showed a more pronounced antibacterial effect, while their in vivo biocompatibility was better than that of the pristine CFR-PEEK, but worse than that of AuNP heterostructures.

Biocompatibility

Noble metal

10.3390/biomedicines10092230

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Метод эндокардиальной инъекции ботулотоксина в ганглионарные сплетения автономной нервной системы сердца в целях снижения уязвимости к фибрилляции предсердий

Patologiya krovoobrashcheniya i kardiokhirurgiya (2016)

А. Г. Стрельников А. А. Якубов David Sergeevichev С. Н. Артеменко I. Mikheenko

Objective. Prior animal studies suggest that botulinum toxin injection into the epicardial fat pads can suppress atrial fibrillation (AF) inducibility. The purpose of the present study was to assess the efficacy and safety of endocardial botulinum toxin injection into epicardial fat pads and intramyocardial left atrial ganglionated plexi (GP) for preventing AF. Methods. Twenty-four dogs were separated into 3 groups: endocardial approach for botulinum toxin (Xeomin, Germany) injection into epicardial fat pads and intramyocardial GPs; endocardial approach for placebo injection (0.9% normal saline; control 1; n = 8) and epicardial approach for botulinum toxin injection (control 2; n = 8). Results. A mean of 6.9±1.7 intramyocardial injections (10 U/0.2 mL at each) and 3 injections (50 U/1 mL at each) were administered into each site exhibiting a positive vagal response and into each epicardial fat pad in all groups (p>0.05 between groups). The injections of botulinum toxin demonstrated dramatic prolongation of ERP in all PV-atrial junctions. This effect correlated with less pronounced ERP shortening in response to vagal nerve stimulation. Suppression of AF inducibility was observed at 7 days after endocardial botulinum toxin injections. The level of AF inducibility was: at 7 days – 57% (p 0.05 for all). The effect of AF suppression disappeared at 3 months. No procedure-related complications occurred. Conclusion. Botulinum toxin injection into intramyocardial GPs and epicardial fat pads by an endocardial approach is feasible and safe. It provides complete removal of cardiac vagal responses and reliably reduces vulnerability to atrial fibrillation.

Epicardial Fat

10.21688/1681-3472-2015-4-99-107

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Different stages of the evolution of cerebral aneurysms: joint analysis of mechanical test data and histological analysis of aneurysm tissue

EPJ Web of Conferences (2019)

D. V. Parshin Anna Lipovka A V Dubovoy Mariya Vasilyeva Еlena Kuznetsova

In practical neurosurgery, an important issue is determining the status of the aneurysm and predicting its further growth, rupture or stabilization. The main approaches for the study of risk analysis asessment are computational hydrodynamics and analysis of the mechanics of the wall of cerebral aneurysm. In this paper, an analysis of various sections of the wall of cerebral aneurysm is given, combining mechanical test data and histological examination data. It was shown that, along with significant diﬀerences in mechanics, a diﬀerent degree of calcification is observed in the tissue, which indicates a diﬀerent level of impaired transport of substances inside the tissue.

Brain tissue

Arterial wall

10.1051/epjconf/201922101028

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Comprehensive study of hexarhenium cluster complex Na 4 [{Re 6 Te 8 }(CN) 6 ] – In terms of a new promising luminescent and X-ray contrast agent

Nanomedicine Nanotechnology Biology and Medicine (2016)

Anna A. Krasilnikova Anastasiya O. Solovieva Anton A. Ivanov Kristina E. Trifonova Tatiana N. Pozmogova

10.1016/j.nano.2016.10.016

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Citations (50)

Distribution of nickel after modified nitinol stent implantation in animals

AIP conference proceedings (2017)

Elena V. Chepeleva David Sergeevichev А. И. Лотков О. А. Кашин A. Korobeynikov

Intravascular stenting of arteries in atherosclerotic lesions is one of the most frequently performed procedures in cardiovascular surgery today. Most stents are made of various stainless-steel alloys and other metals (such as nitinol). Nitinol is a biocompatible, superplastic and corrosion resistant material with an important feature of shape memory. However, the composition of this alloy includes nickel, which shows toxicity to the kidneys, liver, lungs, heart and other organs when it accumulates in the organism. In this research we investigated the nickel content in serum, urine and hair of the laboratory animals after implantation of nitinol stents treated with plasma ionic surface modification by silicon.

Biocompatible material

Nickel titanium

10.1063/1.5001592

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Therapy of Chronic Cardiosclerosis in WAG Rats Using Cultures of Cardiovascular Cells Enriched with Cardiac Stem Cell

Bulletin of Experimental Biology and Medicine (2015)

Elena V. Chepeleva С. В. Павлова Anastasia A. Malakhova E. A. Milevskaya Ya. L. Rusakova

Clonogenic assay

10.1007/s10517-015-3119-y

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Post-implantation endothelization of self-expanding stents from titanium nickelide with the ion-immersion silicon-modified surface in experimental animals

AIP conference proceedings (2018)

M. B. Vasilyeva Elena V. Chepeleva K. Kozyr Dmitry Yu. Zubarev О. А. Кашин

Endovascular prothetics is a frequent procedure in the case of arterial sclerotic disease of the arterial bed of different disposition. The following factors are necessary for the correct performance of the implant: biological intertness and reendothelization of the internal surface of the implanted stent. In the absence of bio-inertness of the constituents of the stent, these components can have a general toxic effect on the patient's body. Incomplete re-endothelization of the inner surface of the stent may cause the formation of intra-stents thrombi (late thrombosis occurring more than 30 days after percutaneous intervention). The presence of such thrombi can dramatically worsen the quality of life of the patient (a life threatening complication). The paper demonstrates that the Ni-Ti stents with the surface modified with silicon ions have higher speed of re-endothelization process than in the case of commercial stents use.

Immersion

10.1063/1.5083558

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