J. Spížek

Czech Academy of Sciences, Institute of Microbiology

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Tomáš Řezanka

Czech Academy of Sciences, Institute of Microbiology

J. Janeček

Czech Academy of Sciences, Institute of Microbiology

Jiřı́ Janata

Czech Academy of Sciences, Institute of Microbiology

P. Tichý

Czech Academy of Sciences, Institute of Microbiology

J. Náprstek

University of Chicago

Jitka Novotná

Czech Geological Survey

Valery M. Dembitsky

Lethbridge College

Vladimı́r Havlı́ček

Czech Academy of Sciences, Institute of Microbiology

Z. Dobrová

Czech Academy of Sciences

Petr Ryšavý

Czech Technical University in Prague

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Czech Academy of Sciences

Czech Academy of Sciences, Institute of Microbiology

Charles University

Institute of Microbiology

Masaryk University

General University Hospital in Prague

Palacký University Olomouc

Centre National de la Recherche Scientifique

University of Chemistry and Technology, Prague

Czech Academy of Sciences, Institute of Molecular Genetics

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LmbJ and LmbIH protein levels correlate with lincomycin production in Streptomyces lincolnensis

Letters in Applied Microbiology (2003)

Klára Holá Jiřı́ Janata Jan Kopecký J. Spížek

To demonstrate the expression of two overlapping genes lmbJ and lmbIH in Streptomyces lincolnensis and to document LmbJ and LmbIH protein levels during the lincomycin production phase. To analyse presumable function of the LmbIH protein.Lincomycin production was monitored by thin-layer chromatography, proteins LmbJ and LmbIH were assayed in the cell-free extracts of S. lincolnensis by immunodetection. LmbJ occurred at stable level (2-4 mg x g(-1) of total proteins) for a long time period (36-96 h of cultivation) covering the whole production phase. This fairly corresponds to the catalytic function of the protein in the antibiotic biosynthesis (N-demethyllincomycin methyltransferase). On the contrary, LmbIH reached the detectable level (0.1 and 0.7 mg x g(-1)) just for a short period at 60-72 h.The absence of LmbIH protein at a detectable level during the major part of the antibiotic production phase casts doubt on its possible catalytic function. Rather a different connection with the final biosynthetic steps, e.g. regulatory, can be envisaged.Expression of a newly found putative regulatory gene was demonstrated during production of industrial antibiotic, lincomycin.

Lincomycin

Streptomycetaceae

Overproduction

10.1046/j.1472-765x.2003.01432.x

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Citations (3)

Access to lincomycin N-oxide isomers controlled by reaction conditions

Tetrahedron Letters (2004)

Stanislav Pospı́šil Petr Sedmera Petr Halada Libor Havlı́ček J. Spížek

Lincomycin

10.1016/j.tetlet.2004.02.074

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Citations (14)

Pilot-plant cultivation of Streptomyces griseus producing homologues of nonactin by precursor-directed biosynthesis and their identification by LC/MS-ESI

The Journal of Antibiotics (2010)

Tomáš Řezanka A. Prell J. Spížek Karel Sigler

Streptomycetaceae

Streptomyces griseus

Isovalerate

Nerol

10.1038/ja.2010.93

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Citations (10)

Overproduction of microbial products—Facts and ideas

Biotechnology Advances (1990)

З. Ванек Z. Hošťálek J. Spížek

Overproduction

Allosteric enzyme

Secondary metabolism

Primary (astronomy)

Metabolic pathway

10.1016/0734-9750(90)90003-t

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Citations (12)

Production and properties of ααα‐glucosidase from the thermotolerant bacterium Thermomonospora curvata

Journal of Applied Microbiology (1997)

Ľubomír Janda T. Pavelka P. Tichý J. Spížek Miroslav Petřı́ček

Journal Article Production and properties of ααα‐glucosidase from the thermotolerant bacteriumThermomonospora curvata Get access L. Janda, L. Janda Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, CzechRepublic Search for other works by this author on: Oxford Academic Google Scholar T. Pavelka, T. Pavelka Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, CzechRepublic Search for other works by this author on: Oxford Academic Google Scholar P. Tichý, P. Tichý Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, CzechRepublic Pavel TichýInstitute of Microbiology, Academy of Sciences of the Czech Republic,Vídeňská 1083, 142 00 Prague 4, Czech Republic (e‐mail :tichy@biomed.cas.cz). Search for other works by this author on: Oxford Academic Google Scholar J. Spížek, J. Spížek Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, CzechRepublic Search for other works by this author on: Oxford Academic Google Scholar M. Petříček M. Petříček Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, CzechRepublic Search for other works by this author on: Oxford Academic Google Scholar Journal of Applied Microbiology, Volume 83, Issue 4, 1 September 1997, Pages 470–476, https://doi.org/10.1046/j.1365-2672.1997.00245.x Published: 01 September 1997 Article history Received: 19 November 1996 Revision received: 07 February 1997 Accepted: 26 February 1997 Published: 01 September 1997

Clinical Microbiology

10.1046/j.1365-2672.1997.00245.x

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Citations (8)

Do we need new antibiotics? The search for new targets and new compounds

Journal of Industrial Microbiology & Biotechnology (2010)

J. Spížek Jitka Novotná Tomáš Řezanka Arnold L. Demain

Pathogenic bacteria

10.1007/s10295-010-0849-8

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Citations (70)

Griseofulvin and other biologically active halogen containing compounds from fungi