Abby Peterson

University of Utah

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Minimal system for assembly of SARS-CoV-2 virus like particles

bioRxiv (Cold Spring Harbor Laboratory) (2020)

Heather Swann Abhimanyu Sharma Benjamin Preece Abby Peterson Crystal Eldridge

Abstract SARS-CoV-2 virus is the causative agent of COVID-19. Here we demonstrate that non-infectious SARS-CoV-2 virus like particles (VLPs) can be assembled by co-expressing the viral proteins S, M and E in mammalian cells. The assembled SARS-CoV-2 VLPs possess S protein spikes on particle exterior, making them ideal for vaccine development. The particles range in shape from spherical to elongated with a characteristic size of 129 ± 32 nm. We further show that SARS-CoV-2 VLPs dried in ambient conditions can retain their structural integrity upon repeated scans with Atomic Force Microscopy up to a peak force of 1 nN.

Virus-like particle

Particle (ecology)

Sars virus

10.1101/2020.06.01.128058

Cite

Citations (6)

Author Correction: Minimal system for assembly of SARS-CoV-2 virus like particles

Scientific Reports (2021)

Heather Swann Abhimanyu Sharma Benjamin Preece Abby Peterson Crystal Eldredge

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

2019-20 coronavirus outbreak

Sars virus

10.1038/s41598-021-88846-9

Cite

Citations (1)

Minimal system for assembly of SARS-CoV-2 virus like particles

Scientific Reports (2020)

Heather Swann Abhimanyu Sharma Benjamin Preece Abby Peterson Crystal Eldredge

SARS-CoV-2 virus is the causative agent of COVID-19. Here we demonstrate that non-infectious SARS-CoV-2 virus like particles (VLPs) can be assembled by co-expressing the viral proteins S, M and E in mammalian cells. The assembled SARS-CoV-2 VLPs possess S protein spikes on particle exterior, making them ideal for vaccine development. The particles range in shape from spherical to elongated with a characteristic size of 129 ± 32 nm. We further show that SARS-CoV-2 VLPs dried in ambient conditions can retain their structural integrity upon repeated scans with Atomic Force Microscopy up to a peak force of 1 nN.

Virus-like particle

2019-20 coronavirus outbreak

Sars virus

10.1038/s41598-020-78656-w

Cite

Citations (64)

Gag-Gag Interactions Are Insufficient to Fully Stabilize and Order the Immature HIV Gag Lattice

Viruses (2021)

Ipsita Saha Benjamin Preece Abby Peterson Haley Durden Brian MacArthur

Immature HIV virions harbor a lattice of Gag molecules with significant ordering in CA-NTD, CA-CTD and SP1 regions. This ordering plays a major role during HIV maturation. To test the condition in which the Gag lattice forms in vivo, we assembled virus like particles (VLPs) by expressing only HIV Gag in mammalian cells. Here we show that these VLPs incorporate a similar number of Gag molecules compared to immature HIV virions. However, within these VLPs, Gag molecules diffuse with a pseudo-diffusion rate of 10 nm

10.3390/v13101946

Cite

Citations (4)