Ivana Vinković Vrček

Institute for Medical Research and Occupational Health

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Ivan Pavičić

Institute for Medical Research and Occupational Health

Mirta Milić

Institute for Medical Research and Occupational Health

Barbara Pem

Rudjer Boskovic Institute

Rinea Barbir

Institute for Medical Research and Occupational Health

Darija Domazet Jurašin

Rudjer Boskovic Institute

Tomislav Vinković

University of Osijek

MĆ

Marija Ćurlin

Catholic University of Croatia

Krunoslav Ilić

Institute for Medical Research and Occupational Health

Valerije Vrček

University of Zagreb

Nikolina Kalčec

Institute for Medical Research and Occupational Health

Cooperative Institutions

University of Zagreb

120

Rudjer Boskovic Institute

University of Osijek

Institute for Medical Research and Occupational Health

Centre National de la Recherche Scientifique

Greek Rescue Team

University of Graz

Agricultural Institute Osijek

National Institute of Chemical Physics and Biophysics

Indiana University School of Medicine

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Biofortifikacija lisnatog povrća selenom - nanobiotehnološki pristup

Tomislav Vinković Boris Ravnjak Ivana Vinković Vrček Zdenko Lončarić Monika Tkalec Kojić

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In vitro protocols for the evaluation of biocompatibility of metallic nanoparticles

Ivana Vinković Vrček Marija Lovrić Lejla Ferhatović Hamzić Daniel Horák Srećko Gajović

Biocompatibility

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OCHRATOXIN A IN ORGANICALLY AND CONVENTIONALLY PRODUCED WINES IN CROATIA

Adela Krivohlavek Željka Kuharić Ana Sekulić Dubravka Vitali Čepo Irena Žuntar

Ochratoxins

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Interaction of silver nanoparticles with cholinesterases

Goran Šinko Ivana Vinković Vrček Snežana Miljanić Adriana Dijanošić

The environmental impact of nanomaterials is expected to increase substantially in the near future as they are becoming a part of our daily life. Silver nanoparticles (AgNP) are widely used in the form of cosmetics, food packaging, drug delivery systems, therapeutics, antimicrobial agents, biosensors, or labels. Manufactured nanoparticles can be toxic via interactions with proteins and enzymes. Nanoparticles are known to be able to interfere with cellular metabolism and to cause cytotoxicity and moreover may interfere with specific cellular functions. We studied interaction of AgNP with acetylcholinesterase (AChE) and related butyrylcholinesterase (BChE). Both cholinesterases are present in brain, blood and nervous system. Acetylcholinesterase (AChE) is a key enzyme in cholinergic neurotransmission and inhibition of AChE may lead to fatal outcome. BChE has important pharmacological and toxicological roles due to its hydrolysis of various xenobiotics. We have evaluated the ChE inhibition by AgNPs prepared using reduction methods of silver nitrate with trisodium citrate (AgNP-citrate), hydroxylamine hydrochloride (AgNP-chloride) and sodium borohydride in presence of polyvinyl alcohol (AgNP-PVA). All tested AgNPs inhibited ChEs and the evaluated apparent inhibitor constants ranged from 49 μM to 670 μM. In the case of AChE the inhibition potency increased in the following order: AgNP-PVA < AgNP-citrate < AgNP-chloride, while in the case of BChE the inhibition potency increased in the following order: AgNP-citrate < AgNP-PVA < AgNP-chloride. The impact of AgNP on ChE activity depends greatly on the nature of the nanomaterial coatings. It is also interesting that AgNP showed higher affinity for AChE, than for BChE.

Silver nanoparticle

Butyrylcholinesterase

Trisodium citrate

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An In Vitro Dosimetry Tool for the Numerical Transport Modeling of Engineered Nanomaterials Powered by the Enalos RiskGONE Cloud Platform

Nanomaterials (2022)

Nikolaos Cheimarios Barbara Pem Andreas Tsoumanis Krunoslav Ilić Ivana Vinković Vrček

A freely available "in vitro dosimetry" web application is presented enabling users to predict the concentration of nanomaterials reaching the cell surface, and therefore available for attachment and internalization, from initial dispersion concentrations. The web application is based on the distorted grid (DG) model for the dispersion of engineered nanoparticles (NPs) in culture medium used for in vitro cellular experiments, in accordance with previously published protocols for cellular dosimetry determination. A series of in vitro experiments for six different NPs, with Ag and Au cores, are performed to demonstrate the convenience of the web application for calculation of exposure concentrations of NPs. Our results show that the exposure concentrations at the cell surface can be more than 30 times higher compared to the nominal or dispersed concentrations, depending on the NPs' properties and their behavior in the cell culture medium. Therefore, the importance of calculating the exposure concentration at the bottom of the cell culture wells used for in vitro arrays, i.e., the particle concentration at the cell surface, is clearly presented, and the tool introduced here allows users easy access to such calculations. Widespread application of this web tool will increase the reliability of subsequent toxicity data, allowing improved correlation of the real exposure concentration with the observed toxicity, enabling the hazard potentials of different NPs to be compared on a more robust basis.

Nanomaterials

10.3390/nano12223935

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Mechanism of 1,2-hydride shift in some carbocations involved in steroid biosynthesis

Croatica Chemica Acta (2001)

Valerije Vrček Ivana Vinković Vrček Olga Kronja

The mechanism of 1,2-hydride shift in protosteryl C(20) cation (1A) and in dammarenyl C(20) cation (2A) was investigated by the semi-empirical AM1 method and ab initio quantum Chemical calculations (HF/3-21G level). Stationary points 1A/1B and 2A/2B, and the corresponding transition hydrido-bridged structures 1TS and 2TS were located on the energy surface. Process 1A→1B turned out to be energetically more favorable than process 2A→2B by ca. 9 kcal mol-1, mostly due to the unfavorable steric repulsive interaction between the methyl group at C(14) and the β-oriented side chain at C(17) in 1A and the lack of CC-hyperconjugative stabilization in 1A. The exothermicity of processes 1A→1B and 2A→2B was increased by subsequent introduction of substituents (H, Me, i-Pr, and t-Bu) at C(14). The more pronounced trend in 1A→1B proves that the origin of the relative stability of 1B comes from the steric interactions in 1A. Introduction of the halogen atom (F, Cl, and Br), due to its -I effect and relatively small size, changed the direction of the equilibrium 1AY/1BY, and 1AY was found to be by ca. 3 kcal mol-1 more stable than 1BY.

Carbocation

Quantum chemical

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Učinak defolijacije na sadržaj fenolnih komponenti u pokožici vinove loze

Hrvoje Rimac Mato Drenjačević Gordana Mendaš Mirza Bojić Ivana Vinković Vrček

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Alteration of Cholinesterase Activity in Daphnia magna After the Exposure to Silver Nanoparticles

Goran Šinko Ivana Vinković Vrček Lea Ulm Adela Krivohlavek