P . Raghava

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Anjali Kumari

Chandigarh University

M . Sathwik

P . Chandrakiran

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Chandigarh University

North Bengal Agricultural University

University of Delhi

Bihar Agricultural University

Indian Institute of Technology Roorkee

Babasaheb Bhimrao Ambedkar Bihar University

ICAR Research Complex for NEH Region

Institute of Engineering

Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya

National Dairy Research Institute

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A Rapid Colorimetric Method for the Determination of Iron in Biological Samples using o-Phenanthroline Method

Indo Global Journal of Pharmaceutical Sciences (2018)

P . Chandrakiran Anjali Kumari M . Sathwik P . Raghava

A simple, sensitive and highly selective direct colorimetric method for the determination of Iron in biological samples is developed.Iron reacts with o-phenanthroline and reduced from Fe3+ to Fe2+ by the use of an excess of hydroxylamine HCl to form a orange red complex ion.It was measured at an absorption maximum of 510 nm.Beer's law was obeyed in the concentration range of 20-200 µg/mL.The method was applied successfully to a number of biological samples (female wistar rats' liver), plant samples (pearl millet seeds).The method is very simple and requires no solvent extraction or pre-concentration steps.

Phenanthroline

Colorimetry

10.35652/igjps.2018.7679

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