Brenda Anguiano

Universidad Nacional Autónoma de México

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Carmen Aceves

Universidad Nacional Autónoma de México

Evangelina Delgado-González

Universidad Nacional Autónoma de México

Guadalupe Delgado

The University of Texas Rio Grande Valley

Julia Rodríguez‐Castelán

Universidad Nacional Autónoma de México

Guadalupe Delgado

Universidad Nacional Autónoma de México

Carlos Montes de

Universidad Nacional Autónoma de México

Olga Cuenca-Micó

Universidad Nacional Autónoma de México

Irasema Mendieta

Autonomous University of Queretaro

Xóchitl Zambrano-Estrada

Autonomous University of Queretaro

Lourdes Rojas Álvarez

Leitat Technological Center

Cooperative Institutions

Universidad Nacional Autónoma de México

Autonomous University of Queretaro

Instituto Mexicano de Oftalmología IAP

Instituto Politécnico Nacional

Instituto Nacional de Neurología y Neurocirugía

Autonomous University of Tlaxcala

Center for Research and Advanced Studies of the National Polytechnic Institute

Instituto Tecnológico de Querétaro

Instituto de Neurologia Y Neurocirugia

Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

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Is Iodine an Antioxidant and Antiproliferative Agent for theMammary and Prostate Glands?

Elsevier eBooks (2009)

Carmen Aceves Brenda Anguiano

10.1016/b978-0-12-374135-6.00026-1

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Citations (11)

Shock Wave Application Increases the Antineoplastic Effect of Molecular Iodine Supplement in Breast Cancer Xenografts

Ultrasound in Medicine & Biology (2019)

Mirle Peña Evangelina Delgado-González Luz M. López-Marı́n Blanca E. Millán‐Chiu F. Fernández

10.1016/j.ultrasmedbio.2019.11.015

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Citations (7)

Neuroendocrine regulation of adrenal gland and hypothalamus 5'deiodinase activity. II. Effects of splanchnicotomy and hypophysectomy.

Endocrinology (1995)

Brenda Anguiano Andrea Sánchez Quintanar Maricela Luna Luz Navarro A. Ramírez del Angel

This study analyzes the role of the autonomic nervous system, the pituitary gland, ACTH, dexamethasone (DEX), and thyroid hormones in the regulation of 5'deiodinase (5'D) in the hypothalamus (HP) and adrenal gland (AG) of the rat. 5'D activity was analyzed in rats under basal conditions (22 C) and during cold exposure (4 C, during 15, 30, 60, and 120 min). Several experimental groups were formed: intact animals (INT), unilateral (left) splanchnicotomized, sham splanchnicotomized, hypophysectomized (HPX), and sham hypophysectomized. Results in the hypothalamus were: 1) independent of the experimental group, the HP 5'D activity increased during the first 15-30 min of cold exposure; however, this increase was greater in operated animals than in INT rats; and 2) basal 5'D activity was increased in HPX rats and was also regulated by thyroid hormones. Results in the adrenal gland were: 1) INT rats exhibited a biphasic pattern of 5'D activation during cold stress (30 and 60 min of exposure); 2) the splanchnic nerve exerted a tonic-stimulatory effect on basal AG 5'D activity; 3) the denervated gland preserved its ability to respond to cold; 4) in INT animals DEX but not ACTH had a stimulatory effect on basal activity; 5) the high 5'D activity post-HPX was reverted to basal values by T4 and DEX administration; 6) SHAM-HPX also was followed by a large increase in basal 5'D activity, and 7) this hyperresponse was abolished by acute ACTH and DEX administration. In conclusion, our results demonstrate that the mechanisms that participate in the regulation and activation of 5'D in the adrenal gland and the hypothalamus are of a neuroendocrine nature. Also, in both organs, but mainly in the HP, 5'D activity is T4-dependent. In addition to the tonic-stimulatory influence conveyed by the splanchnic nerve, AG 5'D activity is influenced by thyroid hormones, glucocorticoids, and probably extrapituitary factors whose nature is unknown yet.

Hypophysectomy

Basal (medicine)

10.1210/endo.136.8.7628370

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Citations (15)

Inhibition of N-methyl-N-nitrosourea-induced mammary carcinogenesis by molecular iodine (I2) but not by iodide (I−) treatment

Molecular and Cellular Endocrinology (2005)

Pablo García‐Solís Yunuen Alfaro Brenda Anguiano Guadalupe Delgado Raphael C. Guzman

Pendrin

10.1016/j.mce.2005.03.001

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Citations (77)

Uptake and antiproliferative effect of molecular iodine in the MCF-7 breast cancer cell line

Endocrine Related Cancer (2006)

Omar Arroyo-Helguera Brenda Anguiano Gabriela Delgado Carmen Aceves

This study analyzes the uptake and antiproliferative effect of two different chemical forms of iodine, iodide (I − ) and molecular iodine (I 2 ), in MCF-7 cells, which are inducible for the Na + /I − symporter (NIS) and positive for pendrin (PDS). The mouse fibroblast cell line NIH3T3 was used as control. Our results show that in MCF-7 cells, I − uptake is sustained and dependent on NIS, whereas I 2 uptake is transient with a maximal peak at 10 min and a final retention of 10% of total uptake. In contrast, no I − was taken up by NIH3T3 cells, and although I 2 was captured with the same time pattern as in MCF-7 cells, its uptake was significantly lower, and it was not retained within the cell. The uptake of I 2 is independent of NIS, PDS, Na + , and energy, but it is saturable and dependent on protein synthesis, suggesting a facilitated diffusion system. Radioiodine was incorporated into protein and lipid fractions only with I 2 treatment. The administration of non-radiolabeled I 2 and 6-iodo-5-hydroxy-8,11,14-eicosatrienoic acid (6-iodolactone, an iodinated arachidonic acid), but not KI, significantly inhibited proliferation of MCF-7 cells. Proliferation of NIH3T3 cells was not inhibited by 20 μM I 2 . In conclusion, these results demonstrate that I 2 uptake does not depend on NIS or PDS; they suggest that in mammary cancer cells, I 2 is taken up by a facilitated diffusion system and then covalently bound to lipids or proteins that, in turn, inhibit proliferation.

MCF-7

Cancer cell lines

10.1677/erc.1.01250

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Citations (74)

Preventive and therapeutic effects of molecular iodine in a model of diabetes mellitus induced by streptozotocin

The Journal of Nutritional Biochemistry (2024)

Julia Rodríguez‐Castelán Evangelina Delgado-González Mónica Sánchez‐Tapia Brenda Anguiano Nimbe Torres

10.1016/j.jnutbio.2024.109783

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Citations (0)

Postejaculatory Increase of Prostatic Triiodothyronine (T3) Depends on Sympathetic Innervation in the Rat1

Biology of Reproduction (2010)

Evangelina Delgado-González Carmen Aceves Brenda Anguiano

Thyronines are essential for the development of the male reproductive system, including the prostate gland. Metabolically active 3,5,3' triiodothyronine (T(3)) is generated mainly by the extrathyroidal, enzymatic 5'deiodination of the prohormone thyroxine (T(4)), which is catalyzed by deiodinases type 1 (D1) and type 2 (D2). Prostate D1 activity is highly expressed during puberty and declines with age, but continuous, long-term sexual activity prevents this reduction. The aims of this study were to characterize the changes in prostatic D1 activity in response to consecutive ejaculations and to determine whether sympathetic input participates in the local T(3) generation (D1 activity). D1 activity was analyzed in prostates of sexually experienced, 4-mo-old male rats after one to five ejaculations. D1 activity, T(3) concentrations, and the T(3)-dependent gene ornithine decarboxylase (Odc) were measured after the fourth ejaculation in prostates of intact, sham, and sympathectomized (Smpx, hypogastric nerve) rats. D1 activity was evaluated by the radio-iodine-release method; T(3) was measured by radioimmunoassay and Odc expression by real-time PCR. Data showed a gradual increase of prostate D1 activity in response to consecutive ejaculations. The highest activity was found after the fourth ejaculation, and it decreased after the fifth. The increase of prostate D1 activity after ejaculation was blocked in Smpx males as compared to intact or sham animals. The changes in D1 activity correlate with prostatic T(3) concentrations and Odc expression. Circulating levels of T(3) were not affected by consecutive ejaculations or by Smpx. These findings indicate that the postejaculatory increase in prostatic generation of T(3) depends on sympathetic input.

Ejaculation

10.1095/biolreprod.110.086116

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Citations (4)

Molecular iodine synergized and sensitized neuroblastoma cells to the antineoplastic effect of ATRA

Endocrine Related Cancer (2020)

Irasema Mendieta Gabriel Rodríguez-Gómez Bertha Rueda-Zarazúa Julia Rodríguez‐Castelán Winniberg Álvarez-León

Neuroblastoma (NB) is the most common solid childhood tumor, and all-trans retinoic acid (ATRA) is used as a treatment to decrease minimal residual disease. Molecular iodine (I 2 ) induces differentiation and/or apoptosis in several neoplastic cells through activation of PPARγ nuclear receptors. Here, we analyzed whether the coadministration of I 2 and ATRA increases the efficacy of NB treatment. ATRA-sensitive (SH-SY5Y), partially-sensitive (SK-N-BE(2)), and non-sensitive (SK-N-AS) NB cells were used to analyze the effect of I 2 and ATRA in vitro and in xenografts (Foxn1 nu/nu mice), exploring actions on cellular viability, differentiation, and molecular responses. In the SH-SY5Y cells, 200 μM I 2 caused a 100-fold (0.01 µM) reduction in the antiproliferative dose of ATRA and promoted neurite extension and neural marker expression (tyrosine hydroxylase (TH) and tyrosine kinase receptor alpha (Trk-A)). In SK-N-AS, the I 2 supplement sensitized these cells to 0.1 μM ATRA, increasing the ATRA-receptor (RARα) and PPARγ expression, and decreasing the Survivin expression. The I 2 supplement increased the mitochondrial membrane potential in SK-N-AS suggesting the participation of mitochondrial-mediated mechanisms involved in the sensibilization to ATRA. In vivo , oral I 2 supplementation (0.025%) synergized the antitumor effect of ATRA (1.5 mg/kg BW) and prevented side effects (body weight loss and diarrhea episodes). The immunohistochemical analysis showed that I 2 supplementation decreased the intratumoral vasculature (CD34). We suggest that the I 2 + ATRA combination should be studied in preclinical and clinical trials to evaluate its potential adjuvant effect in addition to conventional treatments.

Survivin

Tretinoin

10.1530/erc-20-0354

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Citations (5)

Total Iodine Quantification in Fluids and Tissues from Iodine- or Iodide-Supplemented Rats by Ion Chromatography Following Microwave-Assisted Digestion

Thyroid (2015)

Guadalupe Delgado Carolina Muñoz‐Torres Teresa Orozco-Esquivel Brenda Anguiano Carmen Aceves

Iodine is a crucial component of thyroid hormones, and several reports have shown that iodine per se is implicated in the physiopathology of other organs.Innovative ion chromatography detection following a four-step temperature ramp microwave digestion in 25-50 mM nitric acid was developed to measure total iodine in biological fluids and tissue samples from female Sprague-Dawley rats supplemented with 0.05% molecular iodine (I2) or 0.05% potassium iodide (I(-)) in drinking water.The reported method allows the measurement of total iodine with a limit of quantification of 13.7 μg L(-1), recoveries of 96.3-100.3%, and intra- and inter-assay variations, of 3.5% and 7.4% respectively. Analysis of biological fluids showed that after 48 hours, iodine-supplemented animals exhibited significantly higher levels of total iodine in both serum and urine compared with those supplemented with iodide. The half-life of iodine in serum and urine measured over the first 48 h showed similar patterns for both the I2 (7.89 and 7.76 hours) and I(-) (8.27 and 8.90 hours) supplements. Differential uptake patterns were observed in tissues after 6 days of supplements, with I(-) preferentially retained by thyroid, lactating mammary gland, and milk, and a slightly but significantly higher capture of I2 in pituitary, ovary, and virgin mammary gland.We developed a rapid, selective, and accurate digestion method to process fluid and tissue samples that permits reproducible measurements of total iodine by ion chromatography; iodine or iodide supplement show a similar serum and urine half-life, but organ-specific uptake depends on the chemical form of the iodine supplement.

Digestion

10.1089/thy.2014.0290

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Citations (6)

Vestibular site of action of hypothyroidism in the pigmented rat

Brain Research (1990)

Dora Acun ̃a Carmen Aceves Brenda Anguiano Graciela Meza

10.1016/0006-8993(90)90017-6

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Citations (4)