Doris Escalante

Universidad de Ciego de Ávila

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José Carlos Lorenzo

Universidad de Ciego de Ávila

Marcos Edel Martínez-Montero

Universidad de Ciego de Ávila

Elliosha Hajari

Agricultural Research Council of South Africa

Julia Martínez

Universidad de Ciego de Ávila

Sershen Naidoo

University of the Western Cape

Yanier Acosta

Universidad de Ciego de Ávila

Lourdes Yabor

Universidad de Ciego de Ávila

Ariel Villalobos-Olivera

Universidad de Ciego de Ávila

Byron E. Zevallos–Bravo

Universidad Estatal del Sur de Manabi

Dayamí Fontes

Universidad de Ciego de Ávila

Cooperative Institutions

Universidad de Ciego de Ávila

Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria

Weatherford College

University of Granma

Universitat Politècnica de València

Centro de Ingeniería Genética y Biotecnología

Universidad Nacional de Colombia

Instituto de Biología Molecular y Celular de Plantas

Universidad Internacional

Institut de Recherche pour le Développement

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Cryopreservation of Seeds of Neonotonia Wightii Wight & Arn: A Strategy for Conservation, Dormancy Breaking and Preservation of Nutritional Status

Cryoletters (2023)

Yanier Acosta Doris Escalante Marcos Edel Martínez-Montero Dayleni Fortes Byron E. Zevallos–Bravo

BACKGROUND: N. wightii (Leguminosae) is valued as a cover crop and as a potential source of protein in food insecure countries. However, plantlet establishment is limited by physical dormancy. Our previous work has shown that exposure of N. wightii seeds to cryogenic temperatures is able to overcome physical dormancy. OBJECTIVE: The current study is an extension of that work where the field performance and nutritional composition of plants regenerated from N. wightii seeds was investigated. RESULTS: It was evident that plants regenerated from cryopreserved seeds displayed faster growth rates than those from control seeds. In addition, cryopreservation did not alter the nutritional profile of plants produced from cryo-stored seeds. CONCLUSION: Collectively, the results indicate that cryopreservation serves as a suitable strategy for the preservation of seeds of N. wightii with the added benefit of also serving as a dormancy breaking mechanism upon retrieval from cryogenic temperatures.

Plantlet

10.54680/fr23510110712

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Citations (1)

Dormancy breaking in Teramnus labialis (L.f.) Spreng seeds through liquid nitrogen exposure is based on the modification of the hilar region, cuticle, and macrosclereid

Acta Physiologiae Plantarum (2020)

Yanier Acosta Lianny Pérez Doris Escalante Lelurlis Nápoles Oscar Concepción

Imbibition

Cuticle (hair)

10.1007/s11738-020-03134-9

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Citations (5)

Heteromorphic seed germination and seedling emergence in the legume Teramnus labialis (L.f.) Spreng (Fabacaeae)

Botany (2020)

Yanier Acosta Lianny Pérez Doris Escalante A. Pérez Marcos Edel Martínez-Montero

Seed heteromorphism can influence germination and ultimately seedling establishment, particularly in disturbed habitats. This study compared seed and seedling traits across three distinctly colored seed morphs (viz. light-brown, brown, and dark-brown) of the forage legume, Teramnus labialis (L.f.) Spreng. The best quality seeds (i.e., un-parasitized, filled and un-cracked) were brown: 389.3 quality seeds per 1000 units compared with <270/1000 units for the other two morphs. Length, width, volume, and water content were lowest in the light-brown and highest in the dark-brown seeds. Seed thickness and mass were lower in the light-brown seeds. Dark-brown seeds imbibed fastest from 2 h onwards. Germination was comparable across the morphs after 7 days but was lowest in the light-brown (17% at 21 days) and highest in the dark-brown seeds (36% at 21 days) at 14 and 21 days. At 7 days, seedling emergence in the dark-brown seeds (15.0%) was higher than in the other two morphs (4%–6%); this remained so at 14 and 21 days. Seedling growth (number of leaves, stem height and diameter, and root length) was superior in the dark-brown seeds. Seed heteromorphism in T. labialis may allow its persistence in disturbed habitats, and the dark-brown seeds are best suited for seeding in revegetation projects, given their superior germination capacity and seedling vigor.

10.1139/cjb-2020-0008

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Citations (4)

Modifying sugarcane mineral levels through sodium chloride and mannitol exposure in temporary immersion bioreactors

In Vitro Cellular & Developmental Biology - Plant (2019)

Daviel Gómez Julia Martínez Lázaro Hernández Doris Escalante Lourdes Yabor

Osmotic shock

Osmotic pressure

10.1007/s11627-019-10025-3

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Citations (2)

Histology of maize seeds and young germinating embryos after liquid nitrogen exposure

ROMANIAN BIOTECHNOLOGICAL LETTERS (2021)

Ariel Villalobos-Olivera R. E. M. Pereira Daviel Gómez Julia Martínez Doris Escalante

Maize represents a staple food crop and is the second most important agricultural commodity globally. Considering the important role of maize for food security, the long-term conservation of valuable germplasm is critical to ensure that high levels of genetic diversity are available for breeding superior cultivars to face future challenges. Cryopreservation is regarded as the most appropriate tool for long-term germplasm preservation and has been investigated in different crops. This short communication adds to the existing knowledge on maize cryopreservation by describing histological changes observed in maize seeds and young germinating embryos after liquid nitrogen (LN) exposure. Plants were examined immediately after recovery from LN (day zero) and following 3 days of germination. At day 3, seeds exposed to LN showed lower germination rates than non-cryostored seeds, i.e., 60.7% vs. 83.3%. Histological evaluation at day 3 revealed that the thickness of the conical endosperm and the scutellum did not show any statistically significant differences between control and cryopreserved seeds. In contrast, for the other histological evaluations made, mostly regarding the thickness of mesocarp, mealy endosperm, plumule, radicle and the epidermis, significant differences were observed between control and cryostored seeds with the former consistently displaying higher average values than the latter.

Germ plasm

Radicle

Scutellum

10.25083/rbl/26.4/2855-2861

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Effects of Teramnus labialis (L.f.) Spreng seed cryopreservation on subsequent seed and seedling growth and biochemistry

Acta Physiologiae Plantarum (2020)

Yanier Acosta Lianny Pérez Claudia Linares Lázaro Hernández Doris Escalante

Imbibition

Plantlet

Recalcitrant seed

Plant Physiology

10.1007/s11738-020-3012-9

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Citations (13)

Scanning electron microscopy reveals contrasting effects of liquid nitrogen on seeds of legumes Neonotonia wightii, Phaseolus vulgaris and Tamarindus indica

Acta Physiologiae Plantarum (2024)

Yanier Acosta Barbarita Companioni Doris Escalante Byron E. Zevallos–Bravo Lisbet Pérez–Bonachea

Petri dish

Germ plasm

Plantlet

10.1007/s11738-024-03703-2

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Citations (0)

Exposure of Teramnus labialis (L.F.) Spreng Seeds to Liquid Nitrogen Does Not Affect Nutritional Status of Field Grown Adult Plants.

Cryoletters (2021)

Yanier Acosta Luz M. PEREZ Doris Escalante Carlos Mazorra Jorge Martínez Melo

Background Teramnus labialis (L.f.) Spreng is a potentially important legume species, and can be used as an animal feed and to enhance soil physicochemical characteristics. Despite the biological and agricultural importance, the low availability of seeds, their small size and the low percentage germination limit their large-scale use by farmers. We previously reported a method to cryopreserve seeds of T. labialis which also allowed for the breaking of seed dormancy. Objective The current study reports on the nutritional status of 5 month old field grown plants regenerated from cryostored and control seeds. Materials and methods Biomass (fresh and dry mass of leaves and stems) and contents of ash, neutral detergent fiber, acid detergent fiber, lignin, cellulose, crude protein, P, Ca, Mg and K were measured. Results Seeds germinated and emerged faster following immersion in liquid nitrogen (LN) which was supported by quantitative evaluations of fresh and dry weights per m2. However, the ratio of leaf:stem mass were not altered by seed exposure to LN. Conclusion The results showed that exposure of seeds to cryogenic temperatures did not alter the nutritional composition of regenerated plants.

Seed treatment

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Cryopreservation of pineapple shoot tips does not affect mineral contents of regenerated plants

Acta Physiologiae Plantarum (2021)

Ariel Villalobos-Olivera Julia Martínez Doris Escalante Marcos Edel Martínez-Montero Sershen Naidoo

Plant Physiology

10.1007/s11738-020-03195-w

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Citations (3)

Metodología para el control de las contaminaciones en el cultivo in vitro de plantas ornamentales

Cuadernos de fitopatología: Revista técnica de fitopatología y entomología (1996)

María I. Recio Mariela Cid Ruíz Lelurlys Nápoles R. M. González Reinaldo Trujillo Sánchez

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