Edgard Graner

Universidade Estadual de Campinas (UNICAMP)

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Ricardo D. Coletta

Universidade Estadual de Campinas (UNICAMP)

Luiz Paulo Kowalski

AC Camargo Hospital

Débora Campanella Bastos

Universidade Estadual de Campinas (UNICAMP)

Oslei Paes de Almeida

Universidade Estadual de Campinas (UNICAMP)

Márcio Ajudarte Lopes

Universidade Estadual de Campinas (UNICAMP)

Tuula Salo

Hospital District of Helsinki and Uusimaa

Karina G. Zecchin

Hospital of Sobrapar

Michelle Agostini

Universidade Federal do Rio de Janeiro

Sabrina Daniela da Silva

Jewish General Hospital

Hercílio Martelli‐Júnior

Universidade Estadual de Montes Claros

Cooperative Institutions

Universidade Estadual de Campinas (UNICAMP)

187

Universidade de São Paulo

109

Methodist University of Piracicaba

Hospital de Clínicas da Unicamp

Universidade Estadual Paulista (Unesp)

Brazilian Center for Research in Energy and Materials

AC Camargo Hospital

Harvard University

Brazilian Biosciences National Laboratory

Fundação Faculdade de Odontologia

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Sebaceous adenoma of oral cavity: report of case and comparative proliferation study with sebaceous gland hyperplasia and Fordyce's granules

Oral Diseases (2003)

Estela Kaminagakura Cléverton Roberto de Andrade ALCA Rangel Ricardo D. Coletta Edgard Graner

Sebaceous adenoma (SA) is a rare solitary tumour with a predilection for the forehead and scalp. In the English literature, less than 10 cases of SA have been described in the oral cavity. The objective of this study was to examine the clinicopathologic features and evaluate the expression of epidermal growth factor and its receptor, estrogen receptor and androgen receptor in SA and in its differential diagnoses including sebaceous gland hyperplasia (SGH) and Fordyce's granules (FG). Additionally, we analysed the proliferative potential of sebaceous cells from SA, SGH and FG by measuring proliferating cell nuclear antigen (PCNA) expression and quantification of argyrophilic nuclear organizer regions (AgNORs). The SA showed many clinicopathologic similarities to cases previously reported including the biphasic population of cells, in the periphery of lobules undifferentiated basaloid cells whereas the central area was formed by mature sebocytes. SA was composed of 198 lobules of sebaceous cells, whereas SGH and FG showed a mean of 21 ± 7.81 and 5.84 ± 2.83, respectively. The AgNOR and PCNA indices were similar in SA, SGH and FG. These data suggest that lobule counts may be used as additional criteria in distinguishing SA of the oral cavity from other intraoral sebaceous gland lesions.

Sebaceous gland

Sebaceous carcinoma

10.1034/j.1601-0825.2003.00956.x

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Citations (22)

Sturge–Weber syndrome in a 6‐year‐old girl

International Journal of Paediatric Dentistry (2005)

Danyel Elias da Cruz Pérez João Sarmento Pereira Neto Edgard Graner Márcio Ajudarte Lopes

Summary. Sturge–Weber syndrome is a congenital disorder characterized by vascular facial birthmarks and neurological abnormalities. Oral cavity involvement may occur, and the extent of the vascular abnormality may vary considerably. The present authors report the case of a 6‐year‐old girl with Sturge–Weber syndrome, focusing on the clinical and radiographic features. Her dental management involved a multidisciplinary team and included orthodontic treatment using removable appliances.

Birthmark

Sturge–Weber syndrome

Girl

Abnormality

Multidisciplinary team

Congenital disorder

10.1111/j.1365-263x.2005.00595.x

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Citations (15)

Laminin‐induced PC‐12 cell differentiation is inhibited following laser inactivation of cellular prion protein

FEBS Letters (2000)

Edgard Graner Adriana F. Mercadante Sı́lvio M. Zanata Vilma R. Martins Daniel G. Jay

Prions, the etiological agents for infectious degenerative encephalopathies, act by inducing structural modifications in the cellular prion protein (PrPc). Recently, we demonstrated that PrPc binds laminin (LN) and that this interaction is important for the neuritogenesis of cultured hippocampal neurons. Here we have used the PC‐12 cell model to explore the biological role of LN–PrPc interaction. Antibodies against PrPc inhibit cell adhesion to LN‐coated culture plaques. Furthermore, chromophore‐assisted laser inactivation of cell surface PrPc perturbs LN‐induced differentiation and promotes retraction of mature neurites. These results point out to the importance of PrPc as a cell surface ligand for LN.

Neurite

10.1016/s0014-5793(00)02070-6

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Citations (122)

Polymorphisms inGABRB3and Oral Clefting in the Brazilian Population

DNA and Cell Biology (2013)

Marcella Rosa Filézio Elizabete Bagordakis Pinto Sibele Nascimento de Aquino Ana Camila Messetti Hercílio Martelli‐Júnior

The aim of this study was to evaluate the influence of the γ-aminobutyric acid receptor type A β-3 subunit (GABRB3) polymorphisms in patients with nonsyndromic cleft lip and/or palate (NSCL/P). We carried out a structured case–control analysis of three GABRB3 polymorphisms (rs4477673, rs6576618, and rs981778) in 229 patients with nonsyndromic cleft lip with or without cleft palate (CL±P) and in 314 unaffected controls from Brazil. The polymorphisms were genotyped by the TaqMan 5′-exonuclease allelic discrimination assay, and each sample was independently typed for 40 biallelic short insertion/deletion markers (INDELs) to characterize the genomic ancestry. The genotype distributions of the three polymorphisms were as expected by the Hardy–Weinberg equilibrium test. After adjustment to ancestry contribution, the minor A allele of rs981778 was associated with NSCL/P, but significant results did not persist after Bonferroni correction for multiple tests. Similarly, the haplotype analysis revealed that the CCA haplotype (C allele of rs4477673, C allele of rs6576618, and A allele of rs981778) was correlated with NSCL/P, but this association did not remain statistically significant after Bonferroni correction. With a weak association, our data do not support the hypothesis that the GABRB3 variants are a cause of NSCL/P, but further studies are warranted. In nonsyndromic cleft lip and/or palate, three polymorphisms of the GABRB3 gene were investigated for disease association. Only a weak association was found, and the data do not support the hypothesis.

Bonferroni correction

Minor allele frequency

Indel

10.1089/dna.2012.1925

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Citations (6)

The fatty acid synthase inhibitor orlistat reduces experimental metastases and angiogenesis in B16-F10 melanomas

British Journal of Cancer (2012)

Fabiana Seguin Marcos A. Carvalho Débora Campanella Bastos Michelle Agostini Karina G. Zecchin

Fatty acid synthase (FASN) is overexpressed and associated with poor prognosis in several human cancers. Here, we investigate the effect of FASN inhibitors on the metastatic spread and angiogenesis in experimental melanomas and cultured melanoma cells. The lung colonisation assay and cutaneous melanomas were performed by the inoculation of mouse melanoma B16-F10 cells in C57BL6 mice. Blood vessel endothelial cells (RAEC and HUVEC) were applied to determine cell proliferation, apoptosis, and the formation of capillary-like structures. Vascular endothelial growth factor A (VEGFA) expression was evaluated by quantitative RT–PCR and ELISA in B16-F10, human melanoma (SK-MEL-25), and human oral squamous carcinoma (SCC-9) cells. Conditioned media from these cancer cell lines were used to study the effects of FASN inhibitors on endothelial cells. B16-F10 melanoma-induced metastases and angiogenesis were significantly reduced in orlistat-treated mice. Fatty acid synthase inhibitors reduced the viability, proliferation, and the formation of capillary-like structures by RAEC cells, as well as the tumour cell-mediated formation of HUVEC capillary-like structures. Cerulenin and orlistat stimulated the production of total VEGFA in B16-F10, SK-MEL-25, and SCC-9 cells. Both drugs also enhanced VEGFA121, 165, 189, and 165b in SK-MEL-25 and SCC-9 cells. FASN inhibitors reduce metastasis and tumour-induced angiogenesis in experimental melanomas, and differentially modulate VEGFA expression in B16-F10 cells.

Orlistat

Lewis lung carcinoma

Cerulenin

10.1038/bjc.2012.355

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Citations (140)

Differential expression of fatty acid synthase (FAS) and ErbB2 in nonmalignant and malignant oral keratinocytes

Virchows Archiv (2008)

Sabrina Daniela da Silva Isabela Werneck da Cunha Ana Lúcia Carrinho Ayroza Rangel Jacks Jorge Karina G. Zecchin

10.1007/s00428-008-0626-5

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Citations (31)

Oral mucosal health and disease in institutionalized elderly in Brazil

Community Dentistry And Oral Epidemiology (1991)

Jacks Jorge Oslei Paes de Almeida L Bozzo Crispian Scully Edgard Graner

Abstract During the last decade, many studies in Europe and North America have evaluated oral conditions and dental needs in the elderly. Similar information does not exist in Brazil. The aim of this study was to determine the prevalence of oral health and disease in elderly persons living in an institution in the city of Piracicaba, State of Sao Paulo, Brazil. Of 350 elderly persons, 134 males and 136 females were interviewed and clinically examined. Less than one half had healthy mouths. 58.9% of the examined individuals showed one or more lesions in the oral mucosa, the most frequent being denture‐induced stomatitis (20.0%). Fibrous hyperplasias (11.8%) and angular cheilitis (9.3%) were fairly common. Gender, use and degree of hygiene of the denture were factors statistically significantly contributing to prevalence of denture stomatitis. Precancerous lesions such as leukoplakia (3.0%), actinic cheilitis (2.6%) were uncommon but three cases of squamous carcinoma (1.1%) were revealed. This study emphasizes the need for regular oral examinations of the elderly by personnel trained in oral diagnosis.

Elderly people

Oral examination

Oral mucosa

10.1111/j.1600-0528.1991.tb00136.x

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Citations (87)

Influencia da antibioticoterapia sobre a comunidade bacteriana endofítica em microplantas de abacaxizeiro

Resumos (2010)

Gabriela Ferraz Leone Marcílio de Almeida Monita Fiori Abreu-Tarazi Edgard Graner

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Mutual paracrine effects of oral squamous cell carcinoma cells and normal oral fibroblasts: Induction of fibroblast to myofibroblast transdifferentiation and modulation of tumor cell proliferation

Oral Oncology (2007)

Michele Gassen Kellermann Lays Martin Sobral Sabrina Daniela da Silva Karina G. Zecchin Edgard Graner

Transdifferentiation

Myofibroblast

10.1016/j.oraloncology.2007.07.001

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Citations (146)

Integrated Proteomics Identified Up-Regulated Focal Adhesion-Mediated Proteins in Human Squamous Cell Carcinoma in an Orthotopic Murine Model

PLoS ONE (2014)

Daniela Campos Granato Mariana R. Zanetti Rebeca Kawahara Sami Yokoo Romênia R. Domingues

Understanding the molecular mechanisms of oral carcinogenesis will yield important advances in diagnostics, prognostics, effective treatment, and outcome of oral cancer. Hence, in this study we have investigated the proteomic and peptidomic profiles by combining an orthotopic murine model of oral squamous cell carcinoma (OSCC), mass spectrometry-based proteomics and biological network analysis. Our results indicated the up-regulation of proteins involved in actin cytoskeleton organization and cell-cell junction assembly events and their expression was validated in human OSCC tissues. In addition, the functional relevance of talin-1 in OSCC adhesion, migration and invasion was demonstrated. Taken together, this study identified specific processes deregulated in oral cancer and provided novel refined OSCC-targeting molecules.

10.1371/journal.pone.0098208

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Citations (13)