Luísa N. Domingues

Knipling-Bushland U.S. Livestock Insects Research Laboratory

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Felix D. Guerrero

Knipling-Bushland U.S. Livestock Insects Research Laboratory

Arildo Pinto da Cunha

Universidade Federal de Minas Gerais

Kylie G. Bendele

Knipling-Bushland U.S. Livestock Insects Research Laboratory

P. V. B. Leite

Universidade Federal de Minas Gerais

A. C. P. P. Bello

Usmanu Danfodiyo University

Lénaïg Halos

Gates Foundation

Yovany Moreno

Boehringer Ingelheim (United States)

Martin Liebstein

Boehringer Ingelheim (United States)

Antônio Thadeu Medeiros de Barros

Brazilian Agricultural Research Corporation

Monica Figueiredo

Boehringer Ingelheim (United States)

Cooperative Institutions

Universidade Federal de Minas Gerais

Empresa de Pesquisa Agropecuária de Minas Gerais

University of Malaya

Universidade de São Paulo

United States Department of Agriculture

Agricultural Research Service

Universidad de la República

Boehringer Ingelheim (United States)

Knipling-Bushland U.S. Livestock Insects Research Laboratory

Brazilian Agricultural Research Corporation

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Additional file 6 of Identification of anti-horn fly vaccine antigen candidates using a reverse vaccinology approach

Figshare (2021)

Luísa N. Domingues Kylie G. Bendele Lénaïg Halos Yovany Moreno Christian Epe

Additional file 6: Dataset S5. Results of the mass spectrometry analysis. The LC/MS data were searched against multiple custom databases, including the NCBI protein database (4837 horn fly sequences as of January 08, 2018) and the databases used in the in silico analysis.

Reverse vaccinology

Identification

Reverse engineering

10.6084/m9.figshare.16569830

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Additional file 2 of Identification of anti-horn fly vaccine antigen candidates using a reverse vaccinology approach

Figshare (2021)

Luísa N. Domingues Kylie G. Bendele Lénaïg Halos Yovany Moreno Christian Epe

Additional file 2: Dataset S2. Horn fly longest ORFs translated with Virtual Ribosome.

Reverse vaccinology

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10.6084/m9.figshare.16569818

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Influence of selective treatment on Haematobia irritans infestation of untreated cattle

bioRxiv (Cold Spring Harbor Laboratory) (2019)

Cecilia Miraballes Henrik Stryhn Antônio Thadeu Medeiros de Barros Martín Lucas Luísa N. Domingues

Abstract To reduce the use of insecticide treatments against Haematobia irritans we evaluated the impact of treating 15% of the bovines, with the greatest number of flies including bulls, with 40% diazinon ear tags, on the infestation of untreated cows. Horn fly susceptibility to diazinon was measured before and after treatment, and peaks of infestation were recorded. Three groups of Bradford bovines were evaluated: Group 1 (control untreated), Group 2 (15% treated) and Group 3 (control 100% treated). Weekly counts of horn flies were performed on the same animals for 78 days. Two peaks of infestation were recorded, and a higher number of horn flies occurred in the untreated control group than in the untreated cows of the selectively treated group throughout the entire period of the study, except for a single week. The horn fly field population was significantly more susceptible to diazinon than the reference susceptible strain both before and after insecticide treatment. In conclusion, treatment of 15% of the most infested animals from a herd, with 40% diazinon ear tags, quickly reduced horn fly infestations of the entire herd and may be a practical approach for horn fly control, reducing costs and chemical use.

Haematobia irritans

10.1101/798231

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Clinical aspects and dynamics of auricular parasitosis in Gir cattle

Pesquisa Veterinária Brasileira (2013)

P. V. B. Leite L. B. Leite Arildo Pinto da Cunha Marcos Xavier Silva A. C. P. P. Bello

We evaluated the dynamics of ear infestations caused by Rhabditis spp. and Raillietia spp., which were correlated with animal age, intensity of clinical signs and climate factors. Sixty-four Gir cattle were distributed into three groups: GA - 23 calves with 4 to 6 months of age; GB - 18 calves with 7 to 12 months of age; and GC - 23 heifers with 13 to 33 months of age. Five samplings, defined as S1, S2, S3, S4 and S5 were performed every three months from August 2008 to August 2009. The ear secretion was collected using the auricular washing method for the right ear and a swab for the left ear. A clinical assessment of the animals was performed, and they were classified according to the presence and severity of otitis. The highest relative frequency of rhabditosis was 52.2% in GC at the last sampling. In the first sampling, 42.2% of the animals were infested by Raillietia spp. The older cattle were more susceptible to infestations by both parasites. No correlation of Rhabditis spp. and Raillietia spp. parasitism with climate factors was found. The results showed that both parasites could infest Gir cattle, and in most cases, there was no co-infestation. Only older animals parasitized by the nematode showed clinical signs of the disease.

Ear infection

10.1590/s0100-736x2013000300008

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Resistance of the stable fly, Stomoxys calcitrans (Diptera: Muscidae), to cypermethrin in outbreak areas in Midwestern Brazil

Revista Brasileira de Parasitologia Veterinária/Brazilian Journal of Veterinary Parasitology (2019)

Antônio Thadeu Medeiros de Barros Vinícius Duarte Rodrigues Paulo Henrique Duarte Cançado Luísa N. Domingues

Population explosions of the stable fly (Stomoxys calcitrans) have become a serious concern for livestock producers near sugarcane mills in some regions of Brazil due to the insect's massive reproduction on sugarcane byproducts and waste. Despite the limited efficiency of insecticides for controlling stable fly outbreaks, producers still rely on chemical control to mitigate the alarming infestations in affected areas. This study evaluated the susceptibility of S. calcitrans populations to cypermethrin in the state of Mato Grosso do Sul, Brazil. Stable flies were tested from three field populations and two colonies, established from flies previously collected at sugarcane mills. Wild flies were collected with Nzi traps in areas of sugarcane plantations. Both wild and colonized flies were exposed to eleven concentrations of cypermethrin in impregnated filter paper bioassays. All the populations proved to be resistant to cypermethrin, with resistance factors among field populations ranging from 6.8 to 38.6. The intensive use of insecticides has led to the development of pyrethroid resistance in stable fly populations in the proximities of sugarcane mills in the state of Mato Grosso do Sul.

Stable fly

Nuisance

10.1590/s1984-29612019089

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Citations (18)

A reverse vaccinology approach identified novel recombinant tick proteins with protective efficacy against Rhipicephalus microplus infestation

Ticks and Tick-borne Diseases (2024)

Luísa N. Domingues Kylie G. Bendele Deanna Bodine Lénaïg Halos André Antonio Cutolo

The cattle tick, Rhipicephalus microplus, causes significant economic losses to the cattle industry. Tick control is predominately achieved via pesticide applications. However, alternative control methods such as vaccines are needed due to the tick's capacity to quickly develop pesticide resistance and to combat tick-borne diseases. We used an in silico reverse vaccinology approach to evaluate and rank open reading frames (ORFs) from the tick's transcriptome for their potential use as anti-R. microplus vaccine antigens. We manually annotated the 200 highest ranked antigens and selected 10 transcript ORFs as vaccine antigen candidates for expression in Pichia pastoris or insect cells. Six of the ten candidate antigens could be successfully expressed and purified in vitro as recombinant proteins with > 1 mg quantity. RT-PCR confirmed the expression of all six transcripts in tick RNA. However, only three of the six transcripts' corresponding ORFs could be confirmed as present in tick tissue protein extracts. Only four of the six vaccine candidate antigens were successfully expressed and purified in sufficient quantity (> 10 mg) for immunogenicity and efficacy trials in cattle. These four were designated BI-TS002, BI-TS004, BI-TS008, and BI-TS009 and sufficient annotation existed that showed sequence similarity to serine‑rich adhesin for platelets, glycine-rich cell wall structural membrane protein, SWM-1 tick serine protease inhibitor, and venom-like dermonecrotic toxins from ticks and spiders, respectively. Cattle immunized with BI-TS004, BI-TS008 and BI-TS009 yielded a statistically significant difference in antibody response post-immunization. This difference was noted on Days 42, 56, 70, and 84 post-immunization for BI-TS008 and BI-TS009, but only on Day 56 for BI-TS004. BI-TS008 and BI-TS009, were formulated with adjuvant and cattle stall tests conducted over a 175 day period to evaluate efficacy against R. microplus infestations. Both an adjuvant only negative control group and a positive control group using the commercially available GAVAC anti-tick vaccine were used. Efficacy was determined by comparing number of engorged adult female ticks, total egg mass weight, and egg hatchability produced from the immunized group to corresponding data from the adjuvant only negative control group. Thus, effects on engorged adult tick number, reproductive capacity, and fertility were measured. Both initial (designated Phase 1 and calculated from tick collections of Days 60-94 days post-first immunization) and long-term (designated Phase 2 and calculated from tick collections of Days 152-175 post-first immunization) efficacies were determined. The overall Phase 1 trial efficacies of BI-TS008, BI-TS009, and GAVAC were 68.3 %, 48.5 %, and 70.7 %, respectively. The overall Phase 2 trial efficacies of BI-TS008, BI-TS009, and GAVAC were 64.4 %, -30.1 %, and 45.1 %, respectively.

Rhipicephalus microplus

Tick infestation

Rhipicephalus

Reverse vaccinology

10.1016/j.ttbdis.2024.102403

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Additional file 4 of Identification of anti-horn fly vaccine antigen candidates using a reverse vaccinology approach

Figshare (2021)

Luísa N. Domingues Kylie G. Bendele Lénaïg Halos Yovany Moreno Christian Epe

Additional file 4: Dataset S3. Vaxign, Vacceed, and Vaxign results for all ORFs analyzed with these tools, and Gene Ontology, InterPro, BLASTN and BLASTX annotations for the 100 ORFs with highest VaxiJen score.

Reverse vaccinology

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10.6084/m9.figshare.16569824

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Susceptibility of field populations of Haematobia irritans to fipronil in Uruguay

Pesquisa Veterinária Brasileira (2021)

Cecilia Miraballes Antônio Thadeu Medeiros de Barros Martín Lucas Guilherme Klafke Luísa N. Domingues

ABSTRACT: Fipronil was registered in Uruguay in 1997, and, since then, it has been used for the control of Haematobia irritans irritans and Rhipicephalus microplus. The susceptibility of H. irritants to this drug has not been evaluated. Therefore, the goal of the present study was to evaluate the resistance of H. irritans to fipronil. Additionally, a survey was carried out with the farmers to evaluate the use of fipronil for H. irritans control in the ranches where the flies came from. For the bioassays, 31 field populations of H. irritans were exposed to 10 concentrations of fipronil (3.2-16.0μg.cm2), and their LC50 values were calculated using probit analysis. A bioassay was performed with horn flies from the susceptible colony maintained at the USDA-ARS Knipling-Bushland U.S. Livestock Insects Research Laboratory for comparison and calculation of resistance ratios (RRs). All 31 field populations surveyed in the study were susceptible to fipronil, with resistance ratios ranging from <0.5 to 2.2. Four populations with RRs >1 did not differ significantly from the susceptible strain. A single population showed an RR >2.2. Overall, the survey shows that fipronil was mostly used for R. microplus control, and in only three ranches, which were free of R. microplus, was fipronil used for horn fly control. Seventeen farmers did not use fipronil at all in the last three years. It is concluded that, in Uruguay, field populations of horn flies remain susceptible to fipronil.

Fipronil

Haematobia irritans

10.1590/1678-5150-pvb-6821

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Citations (1)

Discovery of the Rdl mutation in association with a cyclodiene resistant population of horn flies, Haematobia irritans (Diptera: Muscidae)

Veterinary Parasitology (2013)

Luísa N. Domingues Felix D. Guerrero Michael E. Becker M. W. Alison Lane D. Foil

Haematobia irritans

Endosulfan

10.1016/j.vetpar.2013.08.023

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Citations (20)

Identification of anti-horn fly vaccine antigen candidates using a reverse vaccinology approach

Parasites & Vectors (2021)

Luísa N. Domingues Kylie G. Bendele Lénaïg Halos Yovany Moreno Christian Epe

Abstract Background The horn fly, Haematobia irritans irritans , causes significant production losses to the cattle industry. Horn fly control relies on insecticides; however, alternative control methods such as vaccines are needed due to the fly's capacity to quickly develop resistance to insecticides, and the pressure for eco-friendly options. Methods We used a reverse vaccinology approach comprising three vaccine prediction and 11 annotation tools to evaluate and rank 79,542 translated open reading frames (ORFs) from the horn fly's transcriptome, and selected 10 transcript ORFs as vaccine candidates for expression in Pichia pastoris . The expression of the 10 selected transcripts and the proteins that they encoded were investigated in adult flies by reverse transcription polymerase chain reaction (RT-PCR) and mass spectrometry, respectively. Then, we evaluated the immunogenicity of a vaccine candidate in an immunization trial and the antigen’s effects on horn fly mortality and fecundity in an in vitro feeding assay. Results Six of the ten vaccine candidate antigens were successfully expressed in P. pastoris . RT-PCR confirmed the expression of all six ORFs in adult fly RNA. One of the vaccine candidate antigens, BI-HS009, was expressed in sufficient quantity for immunogenicity and efficacy trials. The IgG titers of animals vaccinated with BI-HS009 plus adjuvant were significantly higher than those of animals vaccinated with buffer plus adjuvant only from days 42 to 112, with a peak on day 56. Progeny of horn flies feeding upon blood from animals vaccinated with BI-HS009 plus adjuvant collected on day 56 had 63% lower pupariation rate and 57% lower adult emergence than the control group (ANOVA: F (1, 6) = 8.221, P = 0.028 and F (1, 6) = 8.299, P = 0.028, respectively). Conclusions The reverse vaccinology approach streamlined the discovery process by prioritizing possible vaccine antigen candidates. Through a thoughtful process of selection and in vivo and in vitro evaluations, we were able to identify a promising antigen for an anti-horn fly vaccine. Graphical abstract

Reverse vaccinology

Parasitology

Identification

Entomology

10.1186/s13071-021-04938-5

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Citations (6)