Hua YuPing

Northeast Forestry University

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Yanlong Zhang

Xinjiang Production and Construction Corps

Chai HongLiang

Hou ZhiJun

Chinese Academy of Fishery Sciences

Xing MingWei

Lin YueZhi

Zhao Shuchen

Heilongjiang Vocational Institute of Ecological Engineering

Xiaoling Lv

Zhejiang University

Tan TingTing

Zhiping Liu

Changsha University of Science and Technology

Jianhua Zhou

Zhengzhou University of Science and Technology

Cooperative Institutions

Shanxi Agricultural University

Shanghai Jiao Tong University

Northeast Forestry University

Harbin Veterinary Research Institute

Capital Medical University

Northeast Agricultural University

Chinese Academy of Agricultural Sciences

The University of Texas Southwestern Medical Center

Sun Yat-sen University

Central South University

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Diagnosis of hypochromic microcytic anemia of raccoon dog sicken for white nose disease.

Jingji dongwu xuebao (2010)

Hou ZhiJun Zhao Shuchen Xing MingWei Chai HongLiang Li DeJun

To research the pathogenesis of WND the raccoon dog,the Blood Routine Test to WND and normal raccoon dog were done and analyzed by the method of Mann-whinney.Contrast to the normal raccoon dog,the Lymph%(P=0.049) and Gran%(P=0.031) of WND raccoon dog had declined and there were significant difference in those indexes between WND and normal raccoon dog(P0.05).The Lymph#(P=0.004),Min#(P=0.004),Min%(P=0.004),RBC(P=0.000),HCT(P=0.000),MCV(P=0.001),HGB(P=0.000),MCH(P=0.001),RBC(P=0.000),HCT(P=0.000),MCV(P=0.001),HGB(P=0.000) and MCH(P=0.001) of WND raccoon dog had declined and had extreme significant in those indexes between WND and normal raccoon dog(P0.01).WBC(P=0.130),Gran#(P=0.473),MCHC(P=0.915),RDW(P=0.831), RLT(P=0.915),PCT(P=0.852),MPV(P=0.202),PDW(P=0.749) of WND raccoon dog had no difference(P0.05) in those indexes between WND and normal raccoon dog.Based on the parameters of Red Cell Index,RBC,MCH,MCV and MCHV of WND raccoon dog,that the WND raccoon dog sickened for Hypochromic Microcytic Anemia was concluded.

Raccoon Dogs

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Transient immune suppression of inapparent carrier ponies inoculated with attenuated equine infectious anemia virus infectious clone.

Zhongguo yufang shouyi xuebao (2010)

Chenggang Jiang Jian Ma Lin YueZhi Liping Zhao Xiaoling Lv

Equine infectious anemia virus (EIAV) vaccine is the first lentiviral vaccine with a successful application, but itsmechanism on inducing protective immunity remains unclear. Our previous studies found that the gene encoding transmembraneprotein (gp45) of EIAV vaccine strain EIAVFDDV12 had a high-frequent G2230A mutation to create a stop coding, resulting in atruncation of 154 amino acid residues at the C-terminus. To explore the biological meaning of the gp45 truncation, a molecularclone EIAVFDDVTM-36 with truncated gp45 was constructed using EIAVFDDVTM-45 as the backbone. Eight ponies were randomly groupedand inoculated with either EIAVFDDVTM-36 or EIAVFDDVTM-45 and transient immune suppression was induced by daily injection ofdexamethasone for 10 days. The results showed that treatment of dexamethasone did not appear to alter the plasma viral loads andserum neutralizing antibody activities in EIAVFDDVTM-36-inoculated ponies, however it significantly increased plasma viral loads inthree of four EIAVFDDVTM-45-inoculated ponies after the transient immune suppression were observed. Furthermore, the treatment ofdexamethasone significantly enhanced the serum neutralizing activity of the EIAVFDDVTM-45 group of ponies (p0.05). It appeared thatspecific immune pressure had no effect on EIAVFDDVTM-36 replication in vivo, rather limited the production EIAVFDDVTM-45, implicatingan elevated safety of EIAVFDDVTM-36 as an EIAV vaccine. On the other hand, the increased plasma viral loads and a higherneutralization antibody activity in EIAVFDDVTM-45-inoculated ponies indicated that the protection efficacy and the safety of EIAVattenuated vaccines may be negatively correlated. Therefore, it was important to carefully balance these two criteria of lentiviralvaccines.

Equine infectious anemia

Attenuated vaccine

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Serum and hair copper, zinc, iron and selenium concentration of racoon dog with white nose disease.

Zhongguo shouyi xuebao (2010)

Hou ZhiJun Wei Tao Xing MingWei Chai HongLiang Yanlong Zhang

white nose disease (WND) is considered a metabolic disease which may be caused by trace element deficiency but it has not been proved.This study not only builded a method for testing copper,zinc,iron and selenium concentration in serum and hair,but also tested their concentration of normal and WND racoon dog.The copper,zinc,iron and selenium concentration in serum of WND racoon dog were (592.78±110.37) μg /L,(2.019±0.177) mg/L,(28.410±2.826) mg/L and (166.771±38.661) ng/L respectively,significanty loweer than those of the normal racoon dog (709.84±76.56) μg /L,(4.759±1.925) mg/L,(42.352±23.197) mg/L,(303.92±59.527) ng/L (P=0.002,P=0.00,P=0.049 5,P=0.00).Copper,zinc,iron and selenium concentration in hair of WND racoon dog were (17.66±3.61) μg/g,201.43 μg/g,(227.59±143.279) μg/g and (797.429±384.43) ng/g respectively.contrasting with the concentration in hair of normal racoon dog was(16.93±2.86) μg/g,(158.10±31.5) μg/g,(179.613±90.683) μg/g,(612.67±272.410) ng/g respectively,the zine and selenium concentration of hair was significantly lower in WND than that of normal racoondog,(P =0.049 5,P =0.045),copper and iron concentration in the hair had not difference (P=0.395,P=0.236).The result indicated that the white nose disease may relate to a metabolic disorder caused by copper,zinc,iron and selenium.

Selenium deficiency

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Observation on anesthesia effect of Lumianbao in deers

Dongbei Nongye Daxue xuebao (2010)

Hua YuPing

Against deer,wild animal medicine and science's urgent need for anesthesia,Lumianbao was developed as a specific complex anesthetic.Purpose of this experiment was to study the anesthetic effect and the effect on main physiologicalfunction of deer,laying a foundation for the widely use on the clinic,research and the production.Seven deers were injected the Lumianbao by intramuscular injection on neck,0.02 mL.kg-1 by weight,to monitor the anesthesia indexs about conscious-sedation,analgesia and muscle relaxation before and after injection 5,15,30,45,60,75,90,105 min.The results showed that the anaesthesia effect of Lumianbao on deers was definit,with the effectivenesses of conscious-sedation、 analgesia and muscle relaxation balanced,saturation of blood oxygen(above 90%) abundant,and the influences on eupnea and circulation function slight.

Anesthetic Effect

Intramuscular injection

Muscle relaxation

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Analysis on applying foreground of chicken interleukin-2 on poultry intensive cultivation in China

Dongbei Nongye Daxue xuebao (2010)

Hua YuPing

Chicken interleukine-2(IL-2) is a kind of glycoprotein excreted by activated T cells,which significantly enhance the role of the immune.Chicken IL-2,which not only has great action on growth and differentiation of T cells and B cells and on the activating of NK cells,but also has immune effect with other cytokines.It is an immune-regulating factor affecting almost every immunoreaction,so chicken IL-2 has great actions on the therapy of disease.Poultry intensive cultivation integrated the advanced techniques,such as modern genetics and breeding,feeding,epidemic prevention of disease and environment controlling into one package in China.The application of chicken IL-2 may achieve effective combination and integrative utilizing of such techniques.

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A review of sturgeon virosis

Journal of Forestry Research (2005)

Hua YuPing Di Wang

Iridovirus

White (mutation)

10.1007/bf02856862

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Transplantation of Mammarygland of Green Flouorescent Protein Transgenic Mice

Zhongguo Yike Daxue xuebao (2009)

Yanlong Zhang Hua YuPing Tan TingTing Jianzhang Ma

Objective In order to study the development of mammary gland and mechanism of breast cancer,and discuss the technique of and the new animal model of the the transplantation of mammary gland. Methods Using mammary gland tissue of transgenic mice of Green fluorescent protein(GFP),transplanting the mammary gland tissue into the cleared mammary fat pad of wild type mice,8 to 12 weeks later,picking up the mammary fat pad of receipt mice and preparing for the sample of mammary gland tissue,and identified by fluorescence microscope and histology. Results It has been found that GFP mice mammary gland can exist in the fat pad of receipt mice,and differentiate into the structure of duct and alveolar mammary gland. Conclusion The result shows that mice mammary gland of GFP grows in wild type mice gland fat pad for long period. It has provided a new animal model for studying the culture of mammary stem cells,the differentia- tion of mammary gland and the mechanism of breast cancer in the future.

Fat pad

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