Andrea Liliana Onetto

National University of Misiones

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Margarita Ester Laczeski

National University of Misiones

Iliana Julieta Cortese

National University of Misiones

Pedro Darío Zapata

National University of Misiones

GÁ

Gustavo Ángel Bich

Centro Científico Tecnológico - San Juan

María Lorena Castrillo

Centro Científico Tecnológico - San Juan

Fermín Gortari

Centro Científico Tecnológico - La Plata

Fernando Niella

National University of Misiones

Laura Lidia Villalba

Wildlife Conservation Society United Kingdom

Ana Clara López

Universidad de la República

Patricia Rocha

National University of Misiones

Cooperative Institutions

National University of Misiones

Consejo Nacional de Investigaciones Científicas y Técnicas

Universidad Nacional de La Plata

Centro de Investigación y Desarrollo en Criotecnología de Alimentos

Centro Científico Tecnológico - San Juan

Instituto de Biotecnología y Biología Molecular

Universidad Autónoma Monterrey

Centro Científico Tecnológico - Tucumán

Universidad Nacional de Asunción

Universidad Nacional de Itapúa

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Isolation and selection of endophytic spore-forming bacteria with plant growth promoting properties isolated from Ilex paraguariensis St. Hil. (yerba mate)

Figshare (2021)

Margarita Ester Laczeski Andrea Liliana Onetto Iliana Julieta Cortese GISELA Y. MALLOZZI María Lorena Castrillo

Abstract Yerba mate (Ilex paraguariensis St. Hil.) is a species native to the subtropical regions of South America. Despite being an important crop for the region, there are few studies on the use of microorganisms to improve the growth of seedlings in the nursery stage. The objective of this study was to isolate spore-forming endophytic bacteria with plant growth promoting properties associated with yerba mate seedlings and determine their phytobeneficial effect under controlled laboratory conditions. Isolates were selected based on their sporulation capacity and evaluated for in vitro plant growth promoting properties (nitrogen fixation, phosphate solubilization, production of siderophores and synthesis of indolic compounds). Yerba mate seedlings were inoculated with the most promising isolates, which were identified via analyses of the sequence of their 16S rDNA gene as Bacillus circulans (12RS3) and Bacillus altitudinis (19RS3, T5S-T4). After 120 days plants showed higher root dry weight when inoculated with isolate 19RS3 and higher shoot dry weight with 19RS3 and T5S-T4. In conclusion, further studies to determine the ability of these isolates to adapt to the climatic conditions and to survive amidst the native soil microflora in yerba mate cultivated native soils, will be crucial for developing such strains as biofertilizer.

Isolation

10.6084/m9.figshare.14275025

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Tolerancia al estrés de plantines de yerba mate inoculados con bacterias promotoras de crecimiento vegetal nativas de Misiones = Stress tolerance of yerba mate seedlings inoculated with plant growth promoting bacterias native of Misiones

Fermín Gortari Nardia María Luján Bulfe Margarita Ester Laczeski Andrea Liliana Onetto Julieta Cortese

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Screening of antimicrobial activity of Ilex paraguariensis St. Hil. leaf extracts against carbapenemase-producing bacteria

Anais da Academia Brasileira de Ciências (2024)

Andrea Liliana Onetto Marina Novosak DANIANA LILIANA WINNIK Iliana Julieta Cortese PATRICIA ELIZABETH STOCKMANNS

I. paraguariensis St. Hil. is a south American species of agronomic interest with studies supporting its medicinal properties. As the investigation of active ingredients with antimicrobial effect from medicinal plants is a suitable approach to the current antibacterial resistance problem, the aim of the present study was to determine the antibacterial activity of yerba mate ethanolic extracts against carbapenemase-producing gram-negative bacteria (reference strains and clinical isolates). Extracts showed antibacterial activity against Klebsiella pneumoniae ATCC® BAA-2342™ (KPC producing), Providencia rettgeri (NDM producing), Pseudomonas aeruginosa (MBL producing) and P. aeruginosa (VIM producing) at the concentrations tested. The Minimal-Inhibitory-Concentration and Minimal-Bactericidal-Concentration values ranged between 1 and 32 mg.ml-1 for the reference strains, and between 0.125 and 1 mg.ml-1 for the clinical isolates. The MBC/MIC index characterized the extracts as bactericidal. The combinations of commercial antibiotics and extracts showed a synergistic action on the reference strains studied. The lethal concentration 50 obtained using the Artemia salina toxicity assay were higher than 1 mg.ml-1 for all the extracts, indicating a low toxicity. The in vitro activity and low toxicity suggest that ethanolic I. paraguariensis leaf extracts constitute an outstanding source for new antibacterial compounds, and further studies should be carried out to understand their mechanism of action.

10.1590/0001-3765202420221129

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Application of plant growth promoting bacteria enhances the growth of yerba mate (Ilex paraguariensis St. Hil) in greenhouse

Rhizosphere (2022)

Andrea Liliana Onetto Iliana Julieta Cortese Fermín Gortari María Lorena Castrillo Esteban Schegg

10.1016/j.rhisph.2022.100591

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Citations (2)

Isolation and selection of endophytic spore-forming bacteria with plant growth promoting properties isolated from Ilex paraguariensis St. Hil. (yerba mate)

Anais da Academia Brasileira de Ciências (2020)

Margarita Ester Laczeski Andrea Liliana Onetto Iliana Julieta Cortese GISELA Y. MALLOZZI María Lorena Castrillo

Yerba mate (Ilex paraguariensis St. Hil.) is a species native to the subtropical regions of South America. Despite being an important crop for the region, there are few studies on the use of microorganisms to improve the growth of seedlings in the nursery stage. The objective of this study was to isolate spore-forming endophytic bacteria with plant growth promoting properties associated with yerba mate seedlings and determine their phytobeneficial effect under controlled laboratory conditions. Isolates were selected based on their sporulation capacity and evaluated for in vitro plant growth promoting properties (nitrogen fixation, phosphate solubilization, production of siderophores and synthesis of indolic compounds). Yerba mate seedlings were inoculated with the most promising isolates, which were identified via analyses of the sequence of their 16S rDNA gene as Bacillus circulans (12RS3) and Bacillus altitudinis (19RS3, T5S-T4). After 120 days plants showed higher root dry weight when inoculated with isolate 19RS3 and higher shoot dry weight with 19RS3 and T5S-T4. In conclusion, further studies to determine the ability of these isolates to adapt to the climatic conditions and to survive amidst the native soil microflora in yerba mate cultivated native soils, will be crucial for developing such strains as biofertilizer.

Biofertilizer

Dry weight

10.1590/0001-3765202020181381

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Citations (13)

De novo genome assembly of Bacillus altitudinis 19RS3 and Bacillus altitudinis T5S-T4, two plant growth-promoting bacteria isolated from Ilex paraguariensis St. Hil. (yerba mate)

PLoS ONE (2021)

Iliana Julieta Cortese María Lorena Castrillo Andrea Liliana Onetto Gustavo Ángel Bich Pedro Darío Zapata

Plant growth-promoting bacteria (PGPB) are a heterogeneous group of bacteria that can exert beneficial effects on plant growth directly or indirectly by different mechanisms. PGPB-based inoculant formulation has been used to replace chemical fertilizers and pesticides. In our previous studies, two endophytic endospore-forming bacteria identified as Bacillus altitudinis were isolated from roots of Ilex paraguariensis St. Hil. seedlings and selected for their plant growth-promoting (PGP) properties shown in vitro and in vivo . The purposes of this work were to assemble the genomes of B . altitudinis 19RS3 and T5S-T4, using different assemblers available for Windows and Linux and to select the best assembly for each strain. Both genomes were also automatically annotated to detect PGP genes and compare sequences with other genomes reported. Library construction and draft genome sequencing were performed by Macrogen services. Raw reads were filtered using the Trimmomatic tool. Genomes were assembled using SPAdes, ABySS, Velvet, and SOAPdenovo2 assemblers for Linux, and Geneious and CLC Genomics Workbench assemblers for Windows. Assembly evaluation was done by the QUAST tool. The parameters evaluated were the number of contigs ≥ 500 bp and ≥ 1000 bp, the length of the longest contig, and the N50 value. For genome annotation PROKKA, RAST, and KAAS tools were used. The best assembly for both genomes was obtained using Velvet. The B . altitudinis 19RS3 genome was assembled into 15 contigs with an N50 value of 1,943,801 bp. The B . altitudinis T5S-T4 genome was assembled into 24 contigs with an N50 of 344,151 bp. Both genomes comprise several genes related to PGP mechanisms, such as those for nitrogen fixation, iron metabolism, phosphate metabolism, and auxin biosynthesis. The results obtained offer the basis for a better understanding of B . altitudinis 19RS3 and T5S-T4 and make them promissory for bioinoculant development.

Sequence assembly

Bacterial genome size

10.1371/journal.pone.0248274

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Citations (14)

BIOFERTILIZERS AND BIOCONTROLLERS AS AN ALTERNATIVE TO THE USE OF CHEMICAL FERTILIZERS AND FUNGICIDES IN THE PROPAGATION OF YERBA MATE BY MINI-CUTTINGS

Revista Árvore (2019)

Fermín Gortari Maximo Ivan Petruk Nowosad Margarita Ester Laczeski Andrea Liliana Onetto Iliana Julieta Cortese

ABSTRACT The production of yerba mate seedlings through seeds has several limitations, which can be overcome by ex vitro vegetative propagation techniques such as the mini-cuttings, in which it is usually necessary to use synthetic chemical fertilizers and fungicides. However, there is a tendency towards sustainable agriculture, using biofertilizers (growth-promoting bacteria) and biocontrollers (Trichoderma sp.). Therefore, the objectives of this work were to evaluate the effect of biofertilizers on the production of mini-cuttings from yerba mate mini-stumps; as well as the effect, of biocontrollers on survival and rooting capacity of mini-cuttings. Strains of Bacillus sp. and Trichoderma asperelloides of yerba mate were used under two radiation conditions. There was a positive relationship between the availability of radiation and the production of mini-cuttings and the rooting capacity. All the mini-stumps sprouted regardless of treatments. The largest production of viable mini-cuttings occurred in a situation of high radiation and fertilization; while the treatments with growth-promoting bacteria and high radiation had intermediate values. The mini-cuttings inoculated with Trichoderma asperelloides had higher rooting percentage, greater number and length of roots than the mini-cuttings treated with fungicide. Therefore, we demonstrated that the use of chemical products can be replaced by biological ones and achieves acceptable yields.

Cutting

Biofertilizer

Trichoderma

10.1590/1806-90882019000400012

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Citations (12)

BIOFERTILIZERS AND BIOCONTROLLERS AS AN ALTERNATIVE TO THE USE OF CHEMICAL FERTILIZERS AND FUNGICIDES IN THE PROPAGATION OF YERBA MATE BY MINI-CUTTINGS

Figshare (2020)

Fermín Gortari Maximo Ivan Petruk Nowosad Margarita Ester Laczeski Andrea Liliana Onetto Iliana Julieta Cortese

Cutting

Biofertilizer

10.6084/m9.figshare.11900376