Elmar G. Weinhold

G-Quadruplex (G4)-forming DNA sequences have a tendency to form stable multimeric structures. This can be problematic for studies with synthetic oligodeoxynucleotides. Herein, we describe a method that quantitatively converts multimeric intermolecular structures of the Pu27 sequence from the c-myc promoter into the desired monomeric G4 by alkaline treatment and refolding.

Sequence (biology)

G-quadruplex

10.1002/cbic.202000159

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Citations (7)

Inner Sphere and Outer Sphere Electron Transfer to Methyl Iodide. Deuterium and 13C Kinetic Isotope Effects.

Acta chemica Scandinavica/Acta chemica Scandinavica. B, Organic chemistry and biochemistry/Acta chemica Scandinavica. A, Physical and inorganic chemistry/Acta chemica Scandinavica. Series B. Organic chemistry and biochemistry/Acta chemica Scandinavica. Series A, Physical and inorganic chemistry (1996)

Torkil Holm Ingolf Crossland Hans Toftlund Jon Songstad Markku Leskelä

Kinetic isotope effect

10.3891/acta.chem.scand.50-0090

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Citations (7)

S‐Adenosyl‐L‐Methionine and Related Compounds

Christian Dalhoff Elmar G. Weinhold

This chapter contains sections titled: Introduction The Biochemistry of AdoMet The Chemistry and Biochemistry of Modified AdoMet AdoMet as a Pharmaceutical Concluding Remarks References

10.1002/9783527623112.ch9

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Citations (6)

Crystal Structures of Bu4N[Pt(ppy)Cl2], Et4N[Pt(tpy)Cl2] and [Pt(ppy)en]Cl (ppy = N,C'-Chelated 2-Phenylpyridinate, tpy = N,C'-Chelated 2-(2'-Thienyl)pyridinate, en = N,N'-Chelated 1,2-Diaminoethane).

Acta chemica Scandinavica/Acta chemica Scandinavica. B, Organic chemistry and biochemistry/Acta chemica Scandinavica. A, Physical and inorganic chemistry/Acta chemica Scandinavica. Series B. Organic chemistry and biochemistry/Acta chemica Scandinavica. Series A, Physical and inorganic chemistry (1996)

Per-Inge Kvam Thoralf Engebretsen Knut Maartmann-Moe Jon Songstad Markku Leskelä

Crystal (programming language)

10.3891/acta.chem.scand.50-0107

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Citations (14)

Thermodynamic and Kinetic Studies of the Equilibration between the Sulfur- and Carbon-Bonded Forms of the Cobalt(III) Complex with the Ligands 1,4,7-Triazacyclononane and 1,4-Diaza-7-thiacyclodecane.

Acta chemica Scandinavica/Acta chemica Scandinavica. B, Organic chemistry and biochemistry/Acta chemica Scandinavica. A, Physical and inorganic chemistry/Acta chemica Scandinavica. Series B. Organic chemistry and biochemistry/Acta chemica Scandinavica. Series A, Physical and inorganic chemistry (1996)

Yong Sheng Song Jan Becher Pauli Kofod Erik Larsen Johan Springborg

Carbon fibers

10.3891/acta.chem.scand.50-0853

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Citations (6)

A DNA-BINDING PEPTIDE FROM A PHAGE DISPLAY LIBRARY

Nucleosides Nucleotides & Nucleic Acids (2001)

Julian Wölcke Elmar G. Weinhold

A DNA-binding peptide was selected from a random peptide phage display library. For competitive elution using the DNA methyltransferase M.TaqI in the selection step, a biotin-labeled duplex oligodeoxyribonucleotide containing the 5'-TCGA-3' recognition sequence of M.TaqI was employed. Nine of ten phages selected were found to have the same deduced amino acid sequence SVSVGMKPSPRP. The selected phage binds to DNA, as demonstrated in an ELISA.

TaqI

Biopanning

Phagemid

10.1081/ncn-100002526

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Citations (15)

Fine Tuning Antibody Conjugation Methods using SNAP-tag Technology

Anti-Cancer Agents in Medicinal Chemistry (2017)

Karinna Chouman Mira Woitok Radoslav Mladenov Claudia Keßler Elmar G. Weinhold

Targeted imaging and therapy (theranostics) is a promising approach for the simultaneous improvement of cancer diagnosis, prognosis and management. Therapeutic and imaging reagents are coupled to tumor-targeting molecules such as antibodies, providing a basis for truly personalized medicine. However, the development of antibody-drug conjugates with acceptable pharmaceutical properties is a complex process and several parameters must be optimized, such as the controlled conjugation method and the drug-to-antibody ratio.The major aim of this work is to address fundamental key challenges for the development of versatile technology platform for generating homogenous immunotheranostic reagent.We conjugated the theranostics reagent IRDye700dx to a recombinant antibody fusion protein containing a self-labeling protein (SNAP-tag) which provides a unique reaction site.The resulting conjugate was suitable for the imaging of cancer cells expressing the epidermal growth factor receptor and demonstrated potent phototherapeutic and imaging activities against them.Here, we describe a simple, rapid and robust site-directed labeling method that can be used to generate homogeneous immunoconjugate with defined pharmacological properties.

Conjugate

Immunoconjugate

Antibody-drug conjugate

10.2174/1871520617666170213123737

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Nucleophilic Reactions between Cyclic 1,2-Sulfites and Chloride Ions.

Acta chemica Scandinavica/Acta chemica Scandinavica. B, Organic chemistry and biochemistry/Acta chemica Scandinavica. A, Physical and inorganic chemistry/Acta chemica Scandinavica. Series B. Organic chemistry and biochemistry/Acta chemica Scandinavica. Series A, Physical and inorganic chemistry (1996)

Kirsten Nymann Linda Jensen John S. Svendsen T. Anthonsen Ryszard Gawinecki

10.3891/acta.chem.scand.50-0832

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Citations (8)

Expanding the chemical scope of RNA:methyltransferases to site-specific alkynylation of RNA for click labeling

Nucleic Acids Research (2010)

Yuri Motorin Jürgen Burhenne Roman Teimer Kaloian Koynov Sophie Willnow

This work identifies the combination of enzymatic transfer and click labeling as an efficient method for the site-specific tagging of RNA molecules for biophysical studies. A double-activated analog of the ubiquitous co-substrate S -adenosyl- l -methionine was employed to enzymatically transfer a five carbon chain containing a terminal alkynyl moiety onto RNA. The tRNA:methyltransferase Trm1 transferred the extended alkynyl moiety to its natural target, the N2 of guanosine 26 in tRNA Phe . LC/MS and LC/MS/MS techniques were used to detect and characterize the modified nucleoside as well as its cycloaddition product with a fluorescent azide. The latter resulted from a labeling reaction via Cu(I)-catalyzed azide-alkyne 1,3-cycloaddition click chemistry, producing site-specifically labeled RNA whose suitability for single molecule fluorescence experiments was verified in fluorescence correlation spectroscopy experiments.

Moiety

10.1093/nar/gkq825

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Citations (117)

Towards sustained gene-specific silencing of the epithelial cell adhesion molecule

Human Gene Therapy (2007)

Bernardina van der Gun Maria Maluszynska Pamela M.J. McLaughlin Willemijn M. Gommans Alice J. Arendzen

Source

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