Objectives Spinal cord injury (SCI) is an acute traumatic lesion of neurons in the spinal cord which has a high prevalence in the world, and has no effective surgical treatment. HSP70 is a molecular chaperone protein, serves a protective role in several different models of nervous system injury. The aim of the present study was to investigate the anti-inflammatory role of HSP70 in spinal cord injury and explore its mechanism. Methods In vivo and in vitro models were constructed to mimic SCI. The Basso Mouse Scale (BMS) was applied to assess SCI degrees of the mouse model. Immunofluorescence (IF) was used for visualizing HSP70 and Iba1 in the spinal cord. Western blot assay was employed to quantify HSP70 and p65, and ELISA was for IL-1β and TNF-α. Results The results showed that HSP70 expression decreased after SCI. HSP70 and Iba1 showed a decrease of co-localization in SCI mice. Further studies revealed that p65 was upregulated during the process of SCI. Overexpression of HSP70 inhibited the expression of p65 both in vitro and in vivo, and promoted the recovery of SCI mice. Conclusions HSP70 was involved in the pathological process of spinal cord injury, HSP70 alleviated the spinal cord injury via inhibiting NF-κB signaling pathway.
To observe the curative effect of Chinese drugs-paste separated moxibustion combined with electroacupuncture (EA) for knee osteoarthritis (KOA).A total of 124 cases of KOA patients were randomly and equally divided into moxibustion plus electroacupunture (M + EA) group and medication group by using a random table. Chinese drugs-paste separated moxibustion was applied to Shenque (CV 8) for 9 moxa-cones, combined with EA (4 Hz/20 Hz, 1-2 mA) of Liangqiu (ST 34), Heding (EX-LE 2), Neixiyan (EX-LE 4), etc. for 30 min, once daily, 5 sessions a week. Patients of medication group were treated by oral administration of Antine Capsule (diclofenac sodium, 50 mg, twice daily). After 4 weeks of treatment, the therapeutic effect was evaluated.Compared with pre-treatment, the knee-joint pain scores and the index of severity of osteoarthritis of both M + EA and medication groups decreased significantly after termination of the treatment and 10 weeks after the treatment (P < 0.01), and those of M + EA group were markedly lower than those of medication group (P < 0.05). After the treatment, of the two cases in medication and M + EA groups, 3 (4.8%) and 8 (12.9%) were under control in their symptoms, 16 (25.8%) and 21 (33.9%) had remarkable improvement, 31 (50.0%) and 27 (43.5%) were effective, 12 (19.4%) and 6 (9.7%) failed in the treatment, with the effective rates being 80.6% and 90.3% respectively. Ten weeks after the treatment, of the two 62 cases in medication and M + EA groups, 1 (1.6%) and 7 (11.3%) were under control, 10 (16.1%) and 19 (30.6%) had marked improvement, 31 (50.0%) and 28 (45.2%) were effective, 20 (32.3%) and 8 (12.9%) failed in the treatment, with the effective rates being 67.7% and 87.1%, respectively. The curative effect of M + EA group was significantly superior to that of medication group (P < 0.05, P < 0.01).Chinese drugs-paste-separated moxibustion combined with EA is effective in the treatment of KOA of cold-damp type.
// Chao Zu 1 , Shizhang Liu 2 , Wei Cao 1 , Zongzhi Liu 2 , Hui Qiang 2 , Yong Li 2 , Chong Cheng 1 , Le Ji 2 , Jianhui Li 1 and Jingyuan Li 2 1 Department of Surgical Oncology, Shaanxi Provincial People's Hospital, The Third Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710068, Shaanxi Province, P.R. China 2 Department of Orthopaedics, Shaanxi Provincial People's Hospital, The Third Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710068, Shaanxi Province, P.R. China Correspondence to: Jingyuan Li, email: jingyuanli@outlook.com Keywords: intrahepatic cholangiocarcinoma, miR-590-3p, SIP1, metastasis, EMT Received: December 20, 2016 Accepted: February 08, 2017 Published: March 13, 2017 ABSTRACT The functional roles and clinical significances of miR-590-3p in ICC remain unclear. In the current study, we investigated the expression of miR-590-3p in tissues and sera of ICC by real-time quantitative polymerase chain reaction. We found miR-590-3p was significantly down-regulated in the sera and tissues of ICC patients, especially in those patients with lymph node metastasis or distant metastasis. AUC curves and Cox proportional hazards mode revealed serum miR-590-3p could be novel diagnostic and prognostic biomarker for ICC patients. MiR-590-3p dramatically suppressed epithelial-mesenchymal transition, cell migration, and invasion of ICC cells. SIP1 was identified as direct and functional target of miR-590-3p in ICC cells by luciferase assays. Finally, we found SIP1 expression was inversely correlated with miR-590-3p and closely related to diminished survival in ICC patients. These findings reveal functional and mechanistic roles of miR-590-3p and EMT activator SIP1 in the pathogenesis of ICC.
Objectives: To observe the effects of Galectin-1 on the proliferation of cells in the ependymal region of injured spinal cord in adult rats,and to provide a theoretical basis for further study of endogenous neural stem cells in repairment of the injured spinal cord.Methods: 72 healthy female SD rats,with age of 1.5 months and body weight of 190-220g,were divided into sham group(group A),model group(group B),and intervention group(group C).For the later two groups,spinal cord injury models were established on T10,and for the sham group,only laminectomy was performed.The rats in treatment intervention group received sub arachnoid injection of single dose of 20μl(0.2g/L) Galectin-1 24h after injury;the remaining two groups were only given normal saline.Neurological function recovery of the hindlimbs was evaluated using the BBB score 1 day and 7 days after injury,and the expression of BrdU positive cells and Nestin positive cells in spinal cord ependymal zone were detected using immunohistochemical method 7 days after injury.Results: The BBB score of group A was 21.00±0.00 one day after injury.The score of group B(1.65±0.05) showed no significant difference(P0.05) from that of group C(1.59±0.14) one day after injury.The BBB scores of group C(12.98±0.15) and B(6.38±0.21) were significantly different(P0.05) from that of group A(21.00±0.00) 7 days after injury;and the result was not significantly different between group C and B(P0.05).7 days after surgery,very little amount of BrdU positive cells(0.02±0.01) and Nestin positive cells(0.01±0.00) was found in group A;while the amount of BrdU positive cells(0.41±0.14) and Nestin positive cells(0.35±0.13) in group B were abundant and significantly increased(P0.05);Increase in the amount of BrdU positive cells(1.02±0.25) and Nestin positive cells(0.88±0.20) in spinal cord ependymal zone in group C was significantly higher than group B(P 0.05).7d after operation,the BrdU and Nestin integral optical density value in group A were 17.12±3.47 and 10.59±2.11 respectively,group B(32.43±4.96,4.96±7.43) and group C(45.11±6.01,6.01±5.29) had higher value than group A(P0.05),and group C was higher than group B(P0.05).Conclusions: Cells in ependymal area of spinal cord display the potential of proliferation and neural differentiation after injury in rats.Galectin-1 promotes the proliferation and neural differentiation of the cells in this area.
Abstract The composite microgels were synthesized from N ‐Isopropylacrylamide (NIPAM) and acrylic acid (AA) monomers in the presence of graphene oxide (GO) using an in situ radical copolymerization method. The successful preparation of these composite microgels was investigated through Fourier transform infrared spectroscopy (FTIR), ultraviolet visible absorption spectroscopy (UV–vis), and Raman spectroscopy. Due to the hydrophilic properties of GO and the microgels containing oxygenated groups (OH, COOH, and CONH 2 ), quartz crystal microbalance (QCM) sensors can be fabricated by spraying the GO/P(NIPAM ‐co‐ AA) dispersion onto QCM sensors as sensitive coating materials. The results indicate a notable enhancement in the performance of GO/P(NIPAM ‐co‐ AA) modified QCM humidity sensor, compared to QCM sensors modified with either GO or P(NIPAM ‐co‐ AA) microgels alone. This improvement is mainly evidenced by higher sensitivity and reduced moisture hysteresis. The humidity sensing mechanism is based on the combined effect of GO and P(NIPAM ‐co‐ AA) microgels, which synergistically enhance the sensor's performance. Additionally, the results from water contact angle measurements, laser scanning confocal microscopy (LSCM), and scanning electron microscope (SEM) show that GO/P(NIPAM ‐co‐ AA) exhibits greater roughness and stronger hydrophilicity than either GO or P(NIPAM ‐co‐ AA) microgels alone. These properties make GO/P(NIPAM ‐co‐ AA) an effective moisture‐sensitive material for QCM sensors.
High-current pulsed electron beam (HCPEB) technique was applied to irradiate the samples of pure zirconium. The microstructures and defects of the irradiated surface are investigated by using X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). XRD results show that the high value of stress (GPa order) is introduced within the irradiated surface layer, while the formation of {0002}, {1012}, {1120} and {1013} textures are present after HCPEB irradiation. Microstructure observations demonstrate that the surface craters are rarer, and almost no craters are present after multiple pulses HCPEB irradiation, which is evidently different from the case of other metal materials irradiated by HCPEB. Moreover, a large number of ultrafine grains are formed on the irradiated surface. Martensitic transformation occurs and severe plastic deformation is also induced due to the superfast melting and cooling processes. After one- pulse irradiation, the dislocations are the dominant defects, while the amount of twins is less. After five pulses, the dislocation density and the number of deformation twins increase evidently, whereas dense deformation twins are the central microstructures after ten-pulse irradiation, coupled with the appearance of secondary twins occasionally. The formation of these deformed structures results in a significant effect both on the evolution of surface textures and on grain refinement. It is suggested that HCPEB technique provides an impactful approach for hardening of zirconium and zirconium alloys.
Objective:To observe the clinical efficacy of dose-effect relationship of herbs-partitioned moxibustion on Shenque(RN8)for cold accumulation syndrome of dysmenorrhea and its influence on PGF2α and PGE2.Methods:120 cases of moderate cold accumulation syndrome of primary dysmenorrhea were randomly divided into four groups according to the SPSS11.0 statistics software.Another 30 cases were the normal control group.Among them,the observation group A,B and C were treated by herbs-partitioned moxibustion on RN8 and the medicine group was treated by western medicine.Before treatment and after 3 courses,the patients should take the PGF2α and PGE2 tests in 48 hours when the menstruation period.Results:Group A:the total effective rate was 96.7%;group B:the total effective rate was 93.3%;group C:the total effective rate was 90.0%.The medicine group:the total effective rate was 76.7%.Compared with the PGF2α and PGE2 score before and after treatment of observation A,B and C groups(P0.01),there was remarkably statistical significance;compared with the PGF2α and PGE2 score before and after treatment of the control group(P0.05),there was statistics significance.Compared with the PGF2α and PGE2 score after treatment between observation A and B groups,observation B and C groups,observation C and the medicine groups(P0.05),there was statistics significance;Compared the PGF2α and PGE2 score after treatment between observation A and C groups,observation A and the medicine groups,observation B and the medicine groups(P0.01),there was remarkably statistical significance.Conclusion:The clinical efficacy of primary dysmenorrhea treated by herbs-partitioned moxibustion was better than western medicine and large quantity of moxibustion was better than the small one.And the method is convenient,secure,and without adverse reactions.The mechanism has relationship with regulating prostaglandin,which means reducing PGF2α in peripheral blood and raising PGE2 during menstruation.
RNAs are closely associated with human diseases; however, immune-related genes (IRGs) and their potential regulatory networks in relation to spinal cord injury (SCI) are still poorly understood. Here, we investigated the key IRGs as well as the competing endogenous RNA (ceRNA) mechanisms that are associated with SCI pathogenesis based on microarray datasets and the use of a rat SCI model. Specifically, four independent SCI microarray datasets from Gene Expression Omnibus (GEO) database were analyzed and, thereafter, differentially expressed IRGs were annotated via Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Furthermore, based on the GEO datasets, differentially expressed RNAs (DERNAs), including DEcircRNAs, DEmiRNAs, and DEmRNAs were identified and interactions between them were also predicted using online databases, and to construct a circular RNA (circRNA) mediated ceRNA network, candidate RNAs were also identified. Furthermore, the support vector machine (SVM) and least absolute shrinkage and selection operator (LASSO) methods were used for the identification of critical DERNAs, while differential gene expression was validated using the GSE20907 dataset. Our results were as follows. In the SCI microarray datasets, 32, 58, and 74 DEIRGs, DEcircRNAs, and DEmiRNAs were identified, respectively. In addition, GO and KEGG analyses showed that the DEIRGs were primarily enriched in neutrophil-mediated immunity and nuclear factor-kappa B (NF-κB) and hypoxia-inducible factor-1 (HIF-1) signaling pathways, and based on LASSO and SVM screening, PLXNB2 was identified as a DEIRG, while hsa_circ_0026646 was identified as the key circRNA, showing a higher SCI expression. Furthermore, our results proved that PLXNB2 and hsa_circ_0026646 were upregulated in SCI, whereas miR-331-3p was downregulated, and, interestingly, similar expression profiles were confirmed using the rat SCI model. Furthermore, fluorescent reporter assay indicated that both hsa_circ_0026646 and PLXNB2 have miR-331-3p target sites, and the ceRNA hypothesis suggested the dysregulation of hsa_circ_0026646, miR-331-3p, and PLXNB2 in SCI. Thus, our results suggested that in SCI pathogenesis, hsa_circ_0026646 correlates with PLXNB2 by targeting miR-331-3p.
'/%3e%3cuse xlink:href='%23a' transform='rotate(23.036 .093 25.536)'/%3e%3cuse xlink:href='%23a' transform='rotate(45.87 1.273 16.18)'/%3e%3cuse xlink:href='%23a' transform='rotate(69.945 .996 12.078)'/%3e%3cuse xlink:href='%23a' transform='rotate(20.66 -19.689 31.932)'/%3e%3c/svg%3e)