Shao‐Lun Zhai

Guangdong Academy of Agricultural Sciences

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Wen‐Kang Wei

Guangdong Academy of Agricultural Sciences

Dian‐Hong Lv

Guangdong Academy of Agricultural Sciences

Xia Zhou

Guangdong Academy of Agricultural Sciences

Xiao-Hui Wen

Guangzhou Institutes of Biomedicine and Health

Luo Man-lin

South China Agricultural University

Qin-Ling Chen

Guangdong Academy of Agricultural Sciences

Shengnan Chen

Second Xiangya Hospital of Central South University

Tao Lin

China Agricultural University

Ming Liao

South China Agricultural University

Hongchao Gou

Guangdong Academy of Agricultural Sciences

Cooperative Institutions

Chinese Academy of Sciences

Ministry of Agriculture and Rural Affairs

Guangdong Academy of Agricultural Sciences

South China Agricultural University

Chinese Academy of Agricultural Sciences

University of Chinese Academy of Sciences

Zhejiang University

Sun Yat-sen University

Shanghai Jiao Tong University

Chinese Academy of Medical Sciences & Peking Union Medical College

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Can cats become infected with Covid‐19?

Veterinary Record (2020)

Shao‐Lun Zhai Wen‐Kang Wei Dian‐Hong Lv Qi Zhai Xiaohui Wen

Christian ministry

Animal health

10.1136/vr.m1581

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Citations (10)

Development of a multiplex qRT-PCR assay for the detection of porcine epidemic diarrhea virus, porcine transmissible gastroenteritis virus and porcine Deltacoronavirus

Frontiers in Veterinary Science (2023)

Yan Li Jiawei Niu Xia Zhou Pinpin Chu Kunli Zhang

Currently, porcine coronaviruses are prevalent in pigs, and due to the outbreak of COVID-19, porcine coronaviruses have become a research hotspot. porcine epidemic diarrhea virus (PEDV), Transmissible Gastroenteritis Virus (TGEV), and Porcine Deltacoronavirus (PDCoV) mentioned in this study mainly cause diarrhea in pigs. These viruses cause significant economic losses and pose a potential public health threat. In this study, specific primers and probes were designed according to the

Multiplex

10.3389/fvets.2023.1158585

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Citations (9)

The first genome sequence derived from buffalo highly similar to porcine-origin sequences reveals potential cross-species transmission of Seneca Valley virus

Authorea (Authorea) (2020)

Xia Zhou Weifang Liang Dian‐Hong Lv Guangbin Si JH Li

Seneca Valley virus (SVV) strain (SVA/GD/China/2018) was first isolated from a buffalo farm in Guangdong, China, using seven mammalian cell lines. A genetic analysis revealed that SVA/GD/China/2018 had a high nucleotide similarity with the porcine-origin SVV strains, revealing potential cross-species transmission of SVV from pigs to buffaloes.

Strain (injury)

Similarity (geometry)

Sequence (biology)

10.22541/au.160396938.85875907/v1

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Co-existence of multiple strains of porcine circovirus type 2 in the same pig from China

Virology Journal (2011)

Shao‐Lun Zhai Shengnan Chen Zuzhang Wei Jianwu Zhang Lv Huang

Pigs are often co-infected by different viral strains from the same virus. Up to now, there are few reports about co-existence of different porcine circovirus type 2 (PCV2) strains in China. The aim of this study was to evaluate it in Chinese swine herds. 118 PCV2 positive DNAs isolated from diseased pigs identified by classic PCR were re-detected using a modified differential PCR assay. The results indicated that co-existence rates of PCV2 were 32.2% (38/118) in diseased pigs and 0% (0/41) in asymptomatic pigs. Four PCV2 complete genomes were cloned from two co-infected samples and their nucleotide (nt) identities were 95%-97.3%. The phylogenetic analysis showed that four PCV2 strains were divided into different genotypes, PCV2a, PCV2b, PCV2d and PCV2e, respectively. In addition, co-existence were not detected in 41 serum samples from healthy pigs but PCV2 single infection (31.7%, 13/41) existed. These data revealed that the co-existence of different strains of PCV2 might contribute to the development of more severe clinical symptoms for pigs. This is the first report confirming the co-existence of different PCV2 strains in Chinese swine herds. Meanwhile, this study could help us to understand new infection and prevalence forms of PCV2 clinically.

Circovirus

10.1186/1743-422x-8-517

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Citations (52)

A novel porcine kobuvirus emerged in piglets with severe diarrhoea in China

Transboundary and Emerging Diseases (2017)

Shao‐Lun Zhai H. Zhang Tao Lin S.-N. Chen Xia Zhou

Since the first report of porcine kobuvirus (PKV) in 2008, it has been confirmed that PKV is widely present in several countries and plays an important role in diarrhoea outbreak in pigs. Studies have shown that the biggest difference among PKVs is the presence or absence of a specific 30-amino acid (aa) sequence in the 2B region of the polyprotein gene. Based on this unique feature, most PKV sequences could be divided into two groups (Group 1 without deletion and Group 2 with deletion), but a few sequences did not follow this rule due to possible recombination. In this study, two PKV genome sequences, designated JXAT2015 (8,123 nucleotide) and JXJC2015 (8,120 nucleotide), were identified on two different commercial swine farms with the severe diarrhoea outbreak accompanying with highly PKV infection (90%, 18/20) and moderate infection (40%, 8/20) of porcine bocavirus 1 (PBoV1) in Jiangxi province of China. Sequence analysis based on the polyprotein gene showed that they shared low nucleotide similarity (86.3%-88.1%) with other known PKVs. Although both possessed the 30-aa deletion in the 2B region, phylogenetic analysis showed that JXJC2015 was distinct from Group 1 and even Group 2, and formed a new Group (designated Group 3). The findings of this study further revealed genetic diversity and the possible pathogenic role of PKV in conjunction with other pathogens in piglets.

10.1111/tbed.12663

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Citations (13)

Outer membrane vesicles secreted from Actinobacillus pleuropneumoniae isolate disseminating the floR resistance gene to Enterobacteriaceae

Frontiers in Microbiology (2024)

Minsheng Xu Haiyi Ke Zang Ying-an Hongchao Gou Dongxia Yang

, a significant respiratory pig pathogen, is causing substantial losses in the global swine industry. The resistance spectrum of

Actinobacillus pleuropneumoniae

Flor

10.3389/fmicb.2024.1467847

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The influence of Kitchon-RCAA on biomechanics of maxillary tissues based on indirect action: A finite element analysis

Proceedings of the Institution of Mechanical Engineers Part H Journal of Engineering in Medicine (2024)

Jingang Jiang Shao‐Lun Zhai Liang Yao Yongde Zhang Shan Zhou

This paper creates 3D models of Kitchon Root Controlled Auxiliary Archwire (Kitchon-RCAA) with different material properties and assembles them onto the main archwire equipped with brackets. By setting different loading methods and conducting Finite Element Analysis (FEA), the range of Orthodontic Torque/Support Force (OT/SF) values can be obtained. From the obtained values, it can be seen that changes in material properties have a significant impact on the mechanical properties of Kitchon-RCAA. When the properties of the Kitchon-RCAA material change two or more times, the mechanical values generated by Kitchon-RCAA cannot be directly added from two or more separate changes in the properties of the material. Therefore, it is necessary to simulate the model after each parameter change to obtain new results. And then the maxillary bio-model is reconstructed in reverse based on Cone Beam Computerized Tomography (CBCT) images. The biomechanical data equivalent to the mechanical mechanics generated by the root control assisted archwire is also added to the corresponding tooth positions, making indirect orthodontic behavior of Kitchon-RCAA on teeth possible. From the obtained results, it can be seen that the von Mises stress and total deformation magnitude for both normal teeth and corresponding Periodontal Ligament (PDL) position show a stable trend, while the Right Cuspid (R-C) and corresponding PDL with malformed root have a large stress concentration and may have a mold penetration problem. Overall, this paper not only analyses the mechanical behavior of the Kitchon-RCAA, this article not only analyzed the mechanical behavior of Kitchon-RCAA, but also its effect on the indirect biomechanical behavior of the teeth and PDL. And in combination with simulation result nephograms, it also enables predictability and visualization of orthodontic results. This helps dentists to provide safer and more reliable individualized orthodontic treatment plans for patients.

Periodontal fiber

Biomechanics

10.1177/09544119241305468

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Emergence of a novel GIII Getah virus variant in pigs in Guangdong, China, 2023

Microbiology Spectrum (2024)

Pinpin Chu Hongyuan Guo Xia Zhou Shengnan Chen Xinran Sun

From May to July of 2023, one pig farm in Heyuan city, Guangdong Province of China, suffered severe piglet death and sow reproductive disorders. The common pig viruses and bacteria tested negative. To uncover the possible cause of the disease, a metagenomic analysis was performed in the pooled small intestine samples from three 8-day-old diseased piglets. The results showed that Getah virus (GETV), an RNA virus, might be the potential pathogen that affects pig health. Subsequently, GETV nucleotide was detected in all of the 15 samples collected from three diseased piglets using quantitative reverse transcription PCR, suggesting GETV as the main pathogen of the disease. A GETV strain, designated as GDHYLC23, was successfully isolated using the swine testicle cell line. Sequence analysis showed that the epidemic strain had a unique 32-nucleotide repeat insertion in the 3' noncoding region. Phylogenetic analysis showed that GDHYLC23 belonged to the pandemic group III. The identification of GETV with new variations implies the continuous evolution of the virus, which poses potential threats to the swine industry.IMPORTANCEPig farms are faced with emerging and re-emerging viruses that may cause substantial economic loss. The identification of potentially pathogenic viruses helps to prevent and control the spread of diseases. In this study, by using metagenomic analysis, we found that a neglected virus, GETV with a unique insertion in the genome, was the main pathogen in one pig farm that suffered severe piglet death and sow reproductive disorders. Although the potential impact of such an insertion on viral pathogenicity is unknown, the surveillance of the continuing evolution of GETV in pig farms cannot be ignored.

Novel virus

10.1128/spectrum.00483-24

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Citations (3)

Editorial: Emerging swine infectious diseases

Frontiers in Veterinary Science (2023)

Hongchao Gou Xia Zhou Shao‐Lun Zhai

EDITORIAL article Front. Vet. Sci., 19 December 2023Sec. Veterinary Infectious Diseases Volume 10 - 2023 | https://doi.org/10.3389/fvets.2023.1349844

10.3389/fvets.2023.1349844

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Occurrence and sequence analysis of porcine deltacoronaviruses in southern China

Virology Journal (2016)

Shao‐Lun Zhai Wen-Kang Wei Xiaopeng Li Xiao-Hui Wen Xia Zhou

Following the initial isolation of porcine deltacoronavirus (PDCoV) from pigs with diarrheal disease in the United States in 2014, the virus has been detected on swine farms in some provinces of China. To date, little is known about the molecular epidemiology of PDCoV in southern China where major swine production is operated. To investigate the prevalence of PDCoV in this region and compare its activity to other enteric disease of swine caused by porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis coronavirus (TGEV), and porcine rotavirus group C (Rota C), 390 fecal samples were collected from swine of various ages from 15 swine farms with reported diarrhea. Fecal samples were tested by reverse transcription-PCR (RT-PCR) that targeted PDCoV, PEDV, TGEV, and Rota C, respectively. PDCoV was detected exclusively from nursing piglets with an overall prevalence of approximate 1.28 % (5/390), not in suckling and fattening piglets. Interestingly, all of PDCoV-positive samples were from 2015 rather than 2012–2014. Despite a low detection rate, PDCoV emerged in each province/region of southern China. In addition, compared to TGEV (1.54 %, 5/390) or Rota C (1.28 %, 6/390), there were highly detection rates of PEDV (22.6 %, 88/390) in those samples. Notably, all five PDCoV-positive piglets were co-infected by PEDV. Furthermore, phylogenetic analysis of spike (S) and nucleocapsid (N) gene sequences of PDCoVs revealed that currently circulating PDCoVs in southern China were more closely related to other Chinese strains of PDCoVs than to those reported in United States, South Korea and Thailand. This study demonstrated that PDCoV was present in southern China despite the low prevalence, and supported an evolutionary theory of geographical clustering of PDCoVs.

Sequence (biology)

10.1186/s12985-016-0591-6

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Citations (31)