Farahnaz Motamedi-Sedeh

Atomic Energy Organization of Iran

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Homayoon Mahravani

Razi Vaccine and Serum Research Institute

Amir Reza Jalilian

International Atomic Energy Agency

Hoorieh Soleimanjahi

Tarbiat Modares University

Iraj Khalili

Agricultural Research & Education Organization

Viskam Wijewardana

International Atomic Energy Agency

Arash Arbabi

Tehran University of Medical Sciences

Akbar Khorasani

Razi Vaccine and Serum Research Institute

S. Rajabifar

Atomic Energy Organization of Iran

Marzieh Heidarieh

Atomic Energy Organization of Iran

Atefeh Saboorizadeh

Islamic Azad University, Karaj

Cooperative Institutions

Nuclear Science and Technology Research Institute

Atomic Energy Organization of Iran

University of Tehran

Islamic Azad University, Tehran

Agricultural Biotechnology Research Institute of Iran

Tehran University of Medical Sciences

Islamic Azad University, Science and Research Branch

Agricultural Research & Education Organization

Razi Vaccine and Serum Research Institute

Islamic Azad University, Karaj

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Immune Responses Following Administration of Whole Gamma Irradiated SARS-CoV-2 Vaccine Stabilized With Disaccharide Trehalose on Syrian Hamster Model

Research Square (Research Square) (2023)

Farahnaz Motamedi-Sedeh Akbar Khorasani Mohsen Lotfi Seyed Morteza Moosavi Arash Arbabi

Abstract Background The SARS-CoV-2 virus is the causative agent of the emerging respiratory zoonosis disease. One of the most important requirements for the control of emerging diseases is the development of vaccines within a short period of time. Methods The use of ionizing radiation to inactivate pathogens has been developed for the rapid production of effective vaccines. In this study, the SARS-CoV-2 virus was isolated from tracheal swabs of an infected man, confirmed by RT-PCR, and propagated on Vero cells. The SARS-CoV-2 virus was irradiated with 14 kGy gamma radiation to completely inactivate it. Evaluation of the antigenic properties of the spike protein subunit S1 showed that the gamma-irradiated virus samples had intact antigens. The gamma-irradiated SARS-CoV-2 virus and formalin-treated virus were used to immunize Syrian hamsters in four vaccine formulations. Results The titer of neutralizing antibodies increased significantly in all vaccinated groups 3 weeks after the second and third vaccinations. Secretory IgA was examined in nasal lavage and NALT fluids and showed that the concentration of sIgA in irradiated vaccine plus trehalose increased significantly 3 weeks after the second and third vaccinations. The splenic lymphocyte proliferation assay showed a significant increase in all vaccinated hamsters, but the increase was greater in irradiated vaccine plus trehalose and irradiated vaccine plus alum. Conclusion In addition, we can introduce irradiated inactivated vaccine SARS-CoV-2 plus disaccharide trehalose via intranasal route of administration and another irradiated inactivated vaccine SARS-CoV-2 plus alum via subcutaneous route as safe and efficient vaccines against COVID-19 which can stimulate mucosal, humeral and cellular immunity.

Inactivated vaccine

Vero cell

10.21203/rs.3.rs-3405744/v1

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Food irradiation: applications, public acceptance and global trade.

AFRICAN JOURNAL OF BIOTECHNOLOGY (2010)

H Ahari Mostafavi Hadi Fathollahi Farahnaz Motamedi-Sedeh

Food irradiation is the treatment of food products by a definite kind of energy. The process involves exposing the packed or bulked food to the rays of the sun. Food irradiation processing that entails combating post-harvest losses, curtailing food-borne disease and overcoming quarantine barriers has been pursued since the mid-50s. The scientific basis and technological adaptation of the process have been well established more than any other post-harvest food processing techniques. In 1981, the FAO/IAEA/WHO Joint Expert Committees on the wholesomeness of irradiated food (JECFI) concluded, “the irradiation of any food commodity up to an overall average dose of 10 KGy presents no toxicological hazard”. The benefits of irradiation technology in addressing post-harvest food problems are, in some cases, unique and can improve the quality of a number of food products by eliminating the risk of pathogenic contaminants. The potential of this technology has been well perceived in recent years in the wake of food-borne disease caused by pathogenic organisms. In fact, many parts of the world are considering food irradiation as a technological saviour in finding a suitable solution for the problems caused by pathogens in food. Irradiation can be regarded as a useful tool to attain food security in the 21st century. Many consumers have misconceptions about the technology and suppose that it makes food radioactive. But, when the method is explained to them they become normally more in favor of it. Over 50 countries have regulatory approvals in place for irradiation of one or more food products. 30 countries are practically applying this technology for a number of food items.

Food Irradiation

Food Preservation

10.5897/ajb2010.000-3109

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RECOMBINANT VP1 PROTEIN OF FMD VIRUS TYPE O/IRN/2010 AS AN IMMUNOGENIC PEPTIDE EXPRESSION SYSTEM ON GUINEA PIG

Farahnaz Motamedi-Sedeh Homayoon Mahravani Hoorieh Soleimanjahi Lila Asadpour Shima Shafaei

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Author response for "Carboxymethyl chitosan bounded iron oxide nanoparticles and gamma‐irradiated avian influenza subtype H9N2 vaccine to development of immunity on mouse and chicken"

Farahnaz Motamedi-Sedeh Atefeh Saboorizadeh Iraj Khalili Massomeh Sharbatdaran Viskam Wijewardana

Gamma Irradiation

10.1002/vms3.680/v2/response1

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Neutralizing Antibody Response against Inactivated FMDV Type O/IRN/2010 Vaccine by Electron Beam in BALB/C Mice

World Academy of Science, Engineering and Technology, International Journal of Medical and Health Sciences (2014)

Farahnaz Motamedi-Sedeh Sh. Chahardoli Homayoon Mahravani Naser Harzandi Masood Sotoodeh

BALB/c

Antibody response

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Development of Protective Immunity against Inactivated Iranian Isolate of Foot-and-Mouth Disease Virus Type O/IRN/2007 Using Gamma Ray-Irradiated Vaccine on BALB/c Mice and Guinea Pigs

Intervirology (2015)

Farahnaz Motamedi-Sedeh Hoorieh Soleimanjahi Amir Reza Jalilian Homayoon Mahravani Kamalodin Shafaee

Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals and is the most damaging disease of livestock worldwide, leading to great economic losses. The aim of this research was the inactivation of FMDV type O/IRN/1/2007 to produce a gamma ray-irradiated (GRI) vaccine in order to immunize mice and guinea pigs.In this research, the Iranian isolated FMDV type O/IRN/1/2007 was irradiated by gamma ray to prepare an inactivated whole virus antigen and formulated as a GRI vaccine with unaltered antigenic characteristics. Immune responses against this vaccine were evaluated on mice and guinea pigs.The comparison of the immune responses between the GRI vaccine and conventional vaccine did not show any significant difference in neutralizing antibody titer, memory spleen T lymphocytes or IFN-γ, IL-4, IL-2 and IL-10 concentrations (p > 0.05). In contrast, there were significant differences in all of the evaluated immune factors between the two vaccinated groups of mice and negative control mice (p < 0.05). The protective dose 50 for the conventional and GRI vaccines obtained were 6.28 and 7.07, respectively, which indicated the high potency of both vaccines.GRI vaccine is suitable for both routine vaccination and control of FMDV in emergency outbreaks.

Inactivated vaccine

10.1159/000433538

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Immune Response of Gamma-Irradiated Inactivated Bivalent Polio Vaccine Prepared plus Trehalose as a Protein Stabilizer in a Mouse Model

Intervirology (2021)

Maryam Mollaei Alamuti Mehrdad Ravanshad Farahnaz Motamedi-Sedeh Arezoo Nabizadeh Elham Ahmadi

Poliovirus causes paralysis by infecting the nervous system. Currently, 2 types of polio vaccine are given in many countries in polio eradication program including inactivated polio vaccine (IPV) and oral polio vaccine (OPV). Because of OPV-related paralysis, OPV should be replaced by IPV.The aim of this study was to prepare the gamma-irradiated IPV and determine its effectiveness compared with the commercial vaccine (OPV) in the mouse model. The virus titration of OPV was determined and then inactivated by the appropriate dose of gamma radiation into an irradiated vaccine formula. The vaccine was inoculated in BALB/c mice in 2 different formulations of intramuscular injection with 2-week intervals. The level of anti-polio-neutralizing antibody and polio-specific splenocyte proliferation assay were evaluated by collecting the blood samples and spleens of the vaccinated groups with conventional vaccine and irradiated vaccine.There was a significant increase in the neutralizing antibody titration between all of the vaccinated groups and negative control group (A) (p < 0.05). And it shows that the IPV by gamma irradiation has the highest antibody titration. Also, the increasing of stimulation index value in the B* group, F group, and G group was the most against other groups. Furthermore, the neutralizing anti-serum titer and splenic lymphocyte proliferation assay show humoral and cellular immunity were significantly increased in the irradiated vaccine group as compared with conventional group.According to the results, gamma-irradiated IPV could induce humoral and cellular immunity in vaccinated mouse groups, so the irradiated poliovirus could be recommended as a good candidate vaccine to prevent the transport of poliovirus to the central nervous system and thus protect against paralysis.

Humoral immunity

Inactivated Poliovirus Vaccine

Inactivated vaccine

Cellular immunity

Polio vaccine

Splenocyte

10.1159/000515392

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Hematological parameters, antioxidant status, and gene expression of γ-INF and IL-1β in vaccinated lambs fed different type of lipids

Tropical Animal Health and Production (2023)

Morteza Rahimi-Tari Ali Asghar Sadeghi Farahnaz Motamedi-Sedeh Mehdi Aminafshar Mohammad Chamani

Malondialdehyde

Corn oil

10.1007/s11250-023-03585-5

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Sequence Analysis of the Foot and Mouth Disease Virus Type O/IRN/2007 VP1 Gene from Iranian isolate

Folia Biologica (2013)

Hoorieh Soleimanjahi Farahnaz Motamedi-Sedeh Amir Reza Jalilian Homayoon Mahravani

The foot and mouth disease virus (FMDV) causes a vesicular and contagious disease of cloven-hoofed animals. In this study, the virus was isolated from vesicles of the infected cattle using cell culture and serotyped by ELISA test. The extracted RNA from the infected cells was reverse transcribed and amplified using VP1 gene-specific primer pairs by means of one-step RT-PCR. The purified VP1 gene was sub-cloned into the uniqe KpnI and BamHI cloning sites of the pcDNA3.1+ vector. The DH5α strain of E. coli was transformed by the vector. The sequences of sub-cloned FMDV type O/IRN/2007 VP1 were aligned with FMDV type O/UKG/2001 VP1 using MegAlign software. Nucleotide sequence comparisons were made using the BLAST software available from the NCBI website. The amino acid sequences of three sub-cloned FMDV type O/IRN/2007 VP1 were also aligned with three other similar sequences using MegAlign software. Nineteen of the most similar VP1 nucleotide sequences (by BLASTN program), FMDV O/IRN/2007 VP1 sequence, twenty isolates of FMDV-O VP1 in Iran and eight topotypes of FMDV type O were aligned by Mega5 to create a FMDV-O VP1-based sequence similarity tree. The nucleotide sequence comparison indicated that FMDV O/ IRN/2007 VP1 had the greatest nucleotide sequence similarity to the VP1 gene of FMDV O1/Manisa/Turkey/69 (99%), FMDV O1/Manisa/Netherlands (98%) and FMDV O1/Manisa/iso87/Turkey (98%). It was also observed that the highest identity between FMDV O/IRN/2007 VP1 sequence and other nucleotide sequences of FMDV type O VP1 genes isolated in Iran during 1997-2004 was about 91%.

Cloning (programming)

10.14712/fb2013059020093

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Citations (8)

Author response for "Carboxymethyl chitosan bounded iron oxide nanoparticles and gamma‐irradiated avian influenza subtype H9N2 vaccine to development of immunity on mouse and chicken"

Farahnaz Motamedi-Sedeh Atefeh Saboorizadeh Iraj Khalili Massomeh Sharbatdaran Viskam Wijewardana

Gamma Irradiation

10.1002/vms3.680/v3/response1

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