Rafael Ramírez Jiménez

Universidad de Zaragoza

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Rafael Martín‐Rapún

Universidad de Zaragoza

Jesús M. de la Fuente

Universidad de Zaragoza

Darija Domazet Jurašin

Rudjer Boskovic Institute

Ivana Vinković Vrček

Institute for Medical Research and Occupational Health

Rinea Barbir

Institute for Medical Research and Occupational Health

Vida Strasser

Rudjer Boskovic Institute

ÁA

Álvaro Artiga

Universidad Autónoma de Madrid

Andrea Sguassero

Don Carlo Gnocchi Foundation

Rafael Ferritto

Unidades Centrales Científico-Técnicas

Ambra Hernis

Don Carlo Gnocchi Foundation

Cooperative Institutions

Don Carlo Gnocchi Foundation

Universidad Autónoma de Madrid

Consejo Superior de Investigaciones Científicas

University of Milan

Mylan (Switzerland)

Universidad de Zaragoza

Instituto de Nanociencia y Materiales de Aragón

Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine

Instituto de Salud Carlos III

Istituti di Ricovero e Cura a Carattere Scientifico

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Surfactant-Free Synthesis and Scalable Purification of Triangular Gold Nanoprisms with Low Non-Specific Cellular Uptake

Nanomaterials (2020)

Rafael Ramírez Jiménez Álvaro Artiga Scott G. Mitchell Rafael Martín‐Rapún Jesús M. de la Fuente

Gold nanoprisms possess remarkable optical properties that make them useful for medical biotechnology applications such as diagnosis and photothermal therapy. However, shape-selective synthesis of gold nanoprisms is not trivial and typically requires either toxic surfactants or time-consuming purification protocols, which can limit their applicability. Here, we show how triangular gold nanoprisms of different sizes can be purified by precipitation using the non-toxic glutathione ligand, thereby removing the need for toxic surfactants and bottleneck purification techniques. The protocol is amenable for direct scaling up as no instrumentation is required in the critical purification step. The new purification method provides a two-fold increased yield in gold nanoprisms compared to electrophoretic filtration, while providing nanoprisms of similar localized surface plasmon resonance wavelength. Crucially, the gold nanoprisms isolated using this methodology show fewer non-specific interactions with cells and lower cellular internalization, which paves the way for a higher selectivity in therapeutic applications.

10.3390/nano10030539

Cite

Citations (11)

Unexpected reactivity of graphene oxide with DBU and DMF

Journal of Materials Chemistry A (2018)

Rafael Ramírez Jiménez Mário Franco Eduardo Rodrigo Raquel Sainz Rafael Ferritto

Several nitrogen-containing graphene-derived materials have been obtained using DBU and DMF as nitrogen sources, and further employed as heterogeneous catalysts.

Reactivity

10.1039/c8ta03529f

Cite

Citations (15)

Protein glycosylation affects nano-bio interactions depending on particle size and surface functionalization

Rinea Barbir Rafael Ramírez Jiménez Rafael Martín‐Rapún Darija Domazet Jurašin Jesús M. de la Fuente

Surface Modification

Source

Cite

Citations (0)

Interaction of silver and gold nanoparticles with proteins of different glycosylation status

Rinea Barbir Rafael Ramírez Jiménez Rafael Martín‐Rapún Vida Strasser Darija Domazet Jurašin

Source

Cite

Citations (0)

A simple and universal enzyme-free approach for the detection of multiple microRNAs using a single nanostructured enhancer of surface plasmon resonance imaging

Analytical and Bioanalytical Chemistry (2018)

Andrea Sguassero Álvaro Artiga Carlo Morasso Rafael Ramírez Jiménez Rafael Martín‐Rapún

10.1007/s00216-018-1331-0

Cite

Citations (44)

Interaction of Differently Sized, Shaped, and Functionalized Silver and Gold Nanoparticles with Glycosylated versus Nonglycosylated Transferrin

ACS Applied Materials & Interfaces (2021)

Rinea Barbir Rafael Ramírez Jiménez Rafael Martín‐Rapún Vida Strasser Darija Domazet Jurašin

Exposure of nanomaterials (NMs) to biological medium results in their direct interaction with biomolecules and the formation of a dynamic biomolecular layer known as the biomolecular corona. Despite numerous published data on nano-biointeractions, the role of protein glycosylation in the formation, characteristics, and fate of such nano-biocomplexes has been almost completely neglected, although most serum proteins are glycosylated. This study aimed to systematically investigate the differences in interaction of metallic NPs with glycosylated vs nonglycosylated transferrin. To reach this aim, we compared interaction mechanisms between differently sized, shaped, and surface-functionalized silver NMs and gold NMs to commercially available human transferrin (TRF), a glycosylated protein, and to its nonglycosylated recombinant form (ngTRF). Bovine serum albumin (BSA) was also included in the study for comparative purposes. Characterization of NMs was performed using transmission electron microscopy and dynamic and electrophoretic light scattering techniques. Fluorescence quenching and circular dichroism methods were used to evaluate protein binding constants on the nanosurface and conformational changes after the protein–NM interactions, respectively. Competitive binding of TRF, ngTRF, and BSA to the surface of different NMs was evaluated by separating them after extraction from protein corona by gel electrophoresis following quantification with a commercial protein assay. The results showed that the binding strength between NMs and transferrin and the changes in the secondary protein structure largely depend not only on NM physicochemical properties but also on the protein glycosylation status. Data gained by this study highlight the relevance of protein glycosylation for all future design, development, and efficacy and safety assessment of NMs.

Bovine serum albumin

Biomolecule

10.1021/acsami.1c04063

Cite

Citations (24)