Jing Lü

Jiangsu Provincial Center for Disease Control and Prevention

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i-10 index

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Weiguo Cao

Clemson University

Xing‐Quan Zhu

Shanxi Agricultural University

Francis Barany

Cornell University

Nian‐Zhang Zhang

Lanzhou Veterinary Research Institute

Zhen Xu

Chinese Academy of Medical Sciences & Peking Union Medical College

Jianmin Huang

Fuzhou University

Dexin Li

Shandong Agricultural University

Oscar A. Mendez

University of Arizona

Xuming Deng

Jilin University

De‐Pei Liu

Chinese Academy of Medical Sciences & Peking Union Medical College

Cooperative Institutions

Capital Medical University

Chinese Academy of Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

Chinese Academy of Agricultural Sciences

University of Turku

Peking University

Chongqing Medical University

Fujian Medical University

Sichuan University

Southern Medical University

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Rabies Immunization Status of Dogs, Beijing, China

Emerging infectious diseases (2011)

Chao Wang Ying Wang Xiaoyan Du Lin Zeng Gang Nathan Dong

10.3201/eid1706.101590

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Citations (5)

Optimizing Adsorption of 17α-Ethinylestradiol from Water by Magnetic MXene Using Response Surface Methodology and Adsorption Kinetics, Isotherm, and Thermodynamics Studies

Molecules (2021)

Mengwei Xu Chao Huang Jing Lü Zi-Han Wu Xianxin Zhu

Magnetic MXene composite Fe3O4@Ti3C2 was successfully prepared and employed as 17α-ethinylestradiol (EE2) adsorbent from water solution. The response surface methodology was employed to investigate the interactive effects of adsorption parameters (adsorption time, pH of the solution, initial concentration, and the adsorbent dose) and optimize these parameters for obtaining maximum adsorption efficiency of EE2. The significance of independent variables and their interactions were tested by the analysis of variance (ANOVA) and t-test statistics. Optimization of the process variables for maximum adsorption of EE2 by Fe3O4@Ti3C2 was performed using the quadratic model. The model predicted maximum adsorption of 97.08% under the optimum conditions of the independent variables (adsorption time 6.7 h, pH of the solution 6.4, initial EE2 concentration 0.98 mg L−1, and the adsorbent dose 88.9 mg L−1) was very close to the experimental value (95.34%). pH showed the highest level of significance with the percent contribution (63.86%) as compared to other factors. The interactive influences of pH and initial concentration on EE2 adsorption efficiency were significant (p < 0.05). The goodness of fit of the model was checked by the coefficient of determination (R2) between the experimental and predicted values of the response variable. The response surface methodology successfully reflects the impact of various factors and optimized the process variables for EE2 adsorption. The kinetic adsorption data for EE2 fitted well with a pseudo-second-order model, while the equilibrium data followed Langmuir isotherms. Thermodynamic analysis indicated that the adsorption was a spontaneous and endothermic process. Therefore, Fe3O4@Ti3C2 composite present the outstanding capacity to be employed in the remediation of EE2 contaminated wastewaters.

Central composite design

Langmuir adsorption model

Quadratic model

10.3390/molecules26113150

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Citations (12)

Strengthening Aesthetic Education to Improve the Undergraduates' Ouality

Journal of Huizhou University (2007)

Jing Lü

The aesthetic education is the important content of diathesis education,and is also the indispensable important constituent part of comprehensive development in universities.This paper researches emphatically the important status of aesthetic education in the universities' diathesis education and the concrete measure to putting aesthetic education in practice,that is,to strengthen the aesthetic education,consummate undergraduates'quality,perfect undergraduates'personality.

Diathesis

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Exploring the catalytic center of TaqI endonuclease: rescuing catalytic activity by double mutations and Mn2+

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics (2001)

Weiguo Cao Jing Lü

Cleavage (geology)

TaqI

Wild type

10.1016/s0167-4838(01)00149-2

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Citations (4)

Evaluation of human antibodies from vaccinated volunteers for protection against Yersinia pestis infection

Microbiology Spectrum (2024)

Li Zhang Binyang Zheng Jing Lü Haisheng Wu Hailian Wu

has a broad host range and has caused lethal bubonic and pneumonic plague in humans. With the emergence of multiple resistant strains and the potential for biothreat use, there is an urgent need for new therapeutic strategies that can protect populations from natural or deliberate infection. Targeting F1 has been proven to be the main strategy for developing vaccines and therapeutic antibodies, but data on anti-F1 antibodies, especially in humans, are scarce. To date, three human anti-F1 monoclonal antibodies (m252, αF1Ig2, and αF1Ig8) from naive populations have been reported. Here, we constructed an antibody library from vaccinees immunized with the plague subunit vaccine IIa by phage display. The genetic basis, epitopes, and biological functions of the obtained mAbs were assessed and evaluated in plague-challenged mice. Three human mAbs, namely, F3, F19, and F23, were identified. Their biolayer responses were 0.4, 0.6, and 0.6 nm, respectively. The dissociation constants (K

Yersinia pestis

Yersiniosis

10.1128/spectrum.01054-24

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Citations (1)

Mutational Analysis of Endonuclease V from Thermotoga maritima

Biochemistry (2002)

Jianmin Huang Jing Lü Francis Barany Weiguo Cao

Endonuclease V nicks damaged DNA at the second phosphodiester bond 3' to inosine, uracil, mismatched bases, or abasic (AP) sites. Alanine scanning mutagenesis was performed in nine conserved positions of Thermotoga maritima endonuclease V to identify amino acid residues involved in recognition or endonucleolytic cleavage of these diverse substrates. Alanine substitution at D43, E89, and D110 either abolishes or substantially reduces inosine cleavage activity. These three mutants gain binding affinity for binding to double-stranded or single-stranded inosine substrates in the absence of a metal ion, suggesting that these residues may be involved in coordinating catalytic metal ion(s). Y80A, H116A, and, to a lesser extent, R88A demonstrate reduced affinities for double-stranded or single-stranded inosine substrates or nicked products. The lack of tight binding to a nicked inosine product accounts for the increased rate of turnover of inosine substrate since the product release is less rate-limiting. Y80A, R88A, and H116A fail to cleave AP site substrates. Their activities toward uracil substrates are in the following order: H116A > R88A > Y80A. These residues may play a role in substrate recognition. K139A maintains wild-type binding affinity for binding to double-stranded and single-stranded inosine substrate, but fails to cleave AP site and uracil substrate efficiently, suggesting that K139 may play a role in facilitating non-inosine substrate cleavage.

Inosine

Alanine

Uracil

Cleavage (geology)

Phosphodiester bond

Alanine scanning

10.1021/bi015960s

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Citations (27)

Gaussia Luciferase Reporter Assay for Assessment of Gene Delivery Systems in Vivo

Chinese Medical Sciences Journal (2010)

Feng Chen Zhen Xu Jing Lü Xiang Lü Wenli Mu

10.1016/s1001-9294(10)60029-6

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Citations (4)

A Novel Anti-Tumor Inhibitor Identified by Virtual Screen with PLK1 Structure and Zebrafish Assay

PLoS ONE (2013)

Jing Lü Shengchang Xin Huan Meng Matt Veldman David A. Schoenfeld

Polo-like kinase 1 (PLK1), one of the key regulators of mitosis, is a target for cancer therapy due to its abnormally high activity in several tumors. Plk1 is highly conserved and shares a nearly identical 3-D structure between zebrafish and humans. The initial 10 mitoses of zebrafish embryonic cleavages occur every∼30 minutes, and therefore provide a rapid assay to evaluate mitosis inhibitors including those targeting Plk1. To increase efficiency and specificity, we first performed a computational virtual screen of∼60000 compounds against the human Plk1 3-D structure docked to both its kinase and Polo box domain. 370 candidates with the top free-energy scores were subjected to zebrafish assay and 3 were shown to inhibit cell division. Compared to general screen for compounds inhibiting zebrafish embryonic cleavage, computation increased the efficiency by 11 folds. One of the 3 compounds, named I2, was further demonstrated to effectively inhibit multiple tumor cell proliferation in vitro and PC3 prostate cancer growth in Xenograft mouse model in vivo. Furthermore, I2 inhibited Plk1 enzyme activity in a dose dependent manner. The IC50 values of I2 in these assays are compatible to those of ON-01910, a Plk1 inhibitor currently in Phase III clinic trials. Our studies demonstrate that zebrafish assays coupled with computational screening significantly improves the efficiency of identifying specific regulators of biological targets. The PLK1 inhibitor I2, and its analogs, may have potential in cancer therapeutics.

PLK1

Morpholino

10.1371/journal.pone.0053317

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Citations (13)

Effects of Deletion and Site-Directed Mutations on Ligation Steps of NAD⁺-Dependent DNA Ligase: A Biochemical Analysis of BRCA1 C-Terminal Domain

Biochemistry (2004)

Hong Feng Jeremy M. Parker Jing Lü Weiguo Cao

DNA strand joining entails three consecutive steps: enzyme adenylation to form AMP−ligase, substrate adenylation to form AMP−DNA, and nick closure. In this study, we investigate the effects on ligation steps by deletion and site-directed mutagenesis of the BRCA1 C-terminal (BRCT) domain using NAD+-dependent DNA ligase from Thermus species AK16D. Deletion of the BRCT domain resulted in substantial loss of ligation activity, but the mutant was still able to form an AMP−ligase intermediate, suggesting that the defects caused by deletion of the entire BRCT domain occur primarily at steps after enzyme adenylation. The lack of AMP−DNA accumulation by the domain deletion mutant as compared to the wild-type ligase indicates that the BRCT domain plays a role in the substrate adenylation step. Gel mobility shift analysis suggests that the BRCT domain and helix−hairpin−helix subdomain play a role in DNA binding. Similar to the BRCT domain deletion mutant, the G617I mutant showed a low ligation activity and lack of accumulation of AMP−DNA intermediate. However, the G617I mutant was only weakly adenylated, suggesting that a point mutation in the BRCT domain could also affect the enzyme adenylation step. The significant reduction of ligation activity by G634I appears to be attributable to a defect at the substrate adenylation step. The greater ligation of mismatched substrates by G638I is accountable by accelerated conversion of the AMP−DNA intermediate to a ligation product at the final nick closure step. The mutational effects of the BRCT domain on ligation steps in relation to protein−DNA and potential protein−protein interactions are discussed.

DNA Ligases

10.1021/bi049451c

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Citations (25)

Altered gene expression of Na+/Ca2+ exchanger isoforms NCX1, NCX2 and NCX3 in chronic ischemic rat brain

Neuroscience Letters (2002)

Jing Lü Xiaoyong Tong Xiaoliang Wang

10.1016/s0304-3940(02)00905-9

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Citations (9)