Ming Lei

Chinese Academy of Sciences

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Baoli Yao

Xi'an Institute of Optics and Precision Mechanics

105

Shaohui Yan

Xi'an Institute of Optics and Precision Mechanics

Yansheng Liang

Xi'an Jiaotong University

Dan Dan

Xi'an Institute of Optics and Precision Mechanics

Yanlong Yang

James Cook University

Peng Gao

Xidian University

Tong Ye

Ningxia University

Tianyu Zhao

New York University

Junwei Min

Xi'an Institute of Optics and Precision Mechanics

Yingli Wang

Chinese Academy of Sciences

Cooperative Institutions

Chinese Academy of Sciences

University of Chinese Academy of Sciences

Xi'an Institute of Optics and Precision Mechanics

Xi'an Jiaotong University

State Key Laboratory of Transient Optics and Photonics

Shanghai Jiao Tong University

University of Science and Technology of China

Fudan University

Peking University

Jilin University

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Biocompatible Nanotomography of Tightly Focused Light

Nano Letters (2022)

Qi Zhang Jun Yin Yihao Yan Sanyou Chen Bingyan Wei

Tightly focusing a spatially modulated laser beam lays the foundations for advanced optical techniques, such as a holographic optical tweezer and deterministic super-resolution imaging. Precisely mapping the subwavelength features of those highly confined fields is critical to improving the spatial resolution, especially in highly scattering biotissues. However, current techniques characterizing focal fields are mostly limited to conditions such as under a vacuum and on a glass surface. An optical probe with low cytotoxicity and resistance to autofluorescence is the key to achieving in vivo applications. Here, we use a newly emerging quantum reference beacon, the nitrogen-vacancy (NV) center in the nanodiamond, to characterize the focal field of the near-infrared (NIR) laser focus in Caenorhabditis elegans (C. elegans). This biocompatible background-free focal field mapping technique has the potential to optimize in vivo optical imaging and manipulation.

Nanodiamond

Biocompatible material

10.1021/acs.nanolett.1c03905

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Expectation-maximization (EM) Algorithm Based on IMM Filtering with Adaptive Noise Covariance 1)

Ming Lei Chongzhao Han

A novel method under the interactive multiple model (IMM) filtering framework is pre- sented in this paper, in which the expectation-maximization (EM) algorithm is used to identify the process noise covariance Q online. For the existing IMM filtering theory, the matrix Q is determined by means of design experience, but Q is actually changed with the state of the maneuvering target. Meanwhile it is severely influenced by the environment around the target, i.e., it is a variable of time. Therefore, the experiential covariance Q can not represent the influence of state noise in the maneuvering process exactly. Firstly, it is assumed that the evolved state and the initial conditions of the system can be modeled by using Gaussian distribution, although the dynamic system is of a nonlinear measurement equation, and furthermore the EM algorithm based on IMM filtering with the Q identification online is proposed. Secondly, the truncated error analysis is performed. Finally, the Monte Carlo simulation results are given to show that the proposed algorithm outperforms the existing algorithms and the tracking precision for the maneuvering targets is improved efficiently.

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Real-time non-destructive testing of dynamic holograms in doubly doped LiNbO 3 crystals using phase-conjugate technique

Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE (2005)

Liyong Ren Baoli Yao Lili Wang Yongfa Kong Neimule Menke

To test the dynamic photorefractive grating during holographic recording, as usual, the signal wave is blocked from time to time and simultaneously the diffracted beam of the reference wave is tested. This kind of measurement destroys the continuity of the holographic recording and increases the random noise and results in bad repeatability and reliability. In this paper, the phase-conjugate technique is used to overcome the above drawbacks. The reference wave and the object wave are much stronger than the probe beam propagating along the counter-direction of the reference wave. Thus the real-time and non-destructive testing of the dynamic holograms can be obtained by measuring the phase-conjugate wave of the object wave.

Reference beam

Phase conjugation

Repeatability

Diffraction efficiency

10.1117/12.649637

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Optical trapping with cylindrical vector beams

Baoli Yao Shaohui Yan Ming Lei Fei Peng Baiheng Ma

Recent development of cylindrical vector beams prompts its application in optical trapping, which shows more effective and improved trapping efficiency in contrast to the traditional Gaussian beam of spatially homogeneous polarization. Using the T-matrix method and vectorial diffraction theory, we calculated and compared the radiation forces exerted on dielectric particles respectively by the linearly polarized, radially polarized and azimuthally polarized beams. Theoretical calculations show that the radially polarized beam can improve the axial trapping efficiency of high-refractive-index larger particles by reducing the scattering force due to the vanishing axial component of Poynting vector near the focus, while the azimuthally polarized beam can not only steadily trap low-refractive-index small particles at the focus center but also can trap multiple high-refractive-index particles around the focus center in virtue of the hollow-ring configuration. The dependences of the trapping efficiencies on the beam parameters, particle size and the numerical aperture of objective lens are discussed. The performances of optical trapping, manipulating and sorting of biological cells, organelles and various micro-particles are demonstrated.

Optical force

Numerical aperture

Gaussian beam

10.1109/foi.2011.6154814

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Coincidence Theorems in Topological Spaces and Their Applications

Journal of Southwest China Normal University (2006)

Ming Lei

A new coincidence theorem for admissible set-valued mappings is proved in general noncompact topological spaces by using generalized R-KKM mappings. As applications, a section theorem, a best approximation theorem and a minimax inequality are also proved.

Coincidence point

Section (typography)

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Fast-speed structured illumination microscopy

Baoli Yao Ming Lei Dan Dan Xing Zhou Jia Qian

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Multi-color structured illumination microscopy for live cell imaging based on the enhanced image recombination transform algorithm

Biomedical Optics Express (2021)

Tianyu Zhao Huiwen Hao Zhaojun Wang Yansheng Liang Kun Feng

Structured illumination microscopy (SIM) has attracted considerable interest in super-resolution, live-cell imaging because of its low light dose and high imaging speed. Obtaining a high-quality reconstruction image in SIM depends on the precise determination of the parameters of the fringe illumination pattern. The image recombination transform (IRT) algorithm is superior to other algorithms in obtaining the precise initial phase without any approximation, which is promising to provide a considerable solution to address the difficulty of initial phase estimation at low-modulation-depth conditions. However, the IRT algorithm only considers a phase shift of π∕2, which limits its applications in general scenarios. In this letter, we present a general form of IRT algorithm suitable for arbitrary phase shifts, providing a powerful tool for parameter estimation in low signal-to-noise cases. To demonstrate the effectiveness of the enhanced IRT algorithm, we constructed a multicolor, structured illumination microscope and studied at super-resolution, the cargo traffic in HRPE cells, and monitored the movement of mitochondrial structures and microtubules in COS-7 cells. The custom SIM system using the enhanced IRT algorithm allows multicolor capability and a low excitation intensity fluorescence imaging less than 1 W/cm 2 . High-quality super-resolution images are obtained, which demonstrates the utility of this approach in imaging in the life sciences.

SIGNAL (programming language)

10.1364/boe.423171

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Digital holographic microscopy with phase-shift-free structured illumination

Photonics Research (2014)

Juanjuan Zheng Peng Gao Baoli Yao Tong Ye Ming Lei

When structured illumination is used in digital holographic microscopy (DHM), each direction of the illumination fringe is required to be shifted at least three times to perform the phase-shifting reconstruction. In this paper, we propose a scheme for spatial resolution enhancement of DHM by using the structured illumination but without phase shifting. The structured illuminations of different directions, which are generated by a spatial light modulator, illuminate the sample sequentially in the object plane. The formed object waves interfere with a reference wave in an off-axis configuration, and a CCD camera records the generated hologram. After the object waves are reconstructed numerically, a synthetic aperture is performed by an iterative algorithm to enhance the spatial resolution. The resolution improvement of the proposed method is proved and demonstrated by both simulation and experiment.

Digital Holographic Microscopy

Digital holography

Phase imaging

10.1364/prj.2.000087

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Citations (34)

Transport properties of random media composed of core-shell spheres

Applied Physics A (2013)

Yuchen Xu Hao Zhang Xiangchao Zhang Yongqiang Shen Heyuan Zhu

10.1007/s00339-013-7862-5

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High Resolution CryoSEM: Examination of Microbial Samples in an In-lens and a Below-the-lens FESEM

Microscopy and Microanalysis (2003)

Stanley L. Erlandsen Ming Lei Chris Frethem C. L. Wells

Journal Article High Resolution CryoSEM: Examination of Microbial Samples in an In-lens and a Below-the-lens FESEM Get access S L Erlandsen, S L Erlandsen Departments of Genetics, Cell Biology, and Development Search for other works by this author on: Oxford Academic Google Scholar M Lei, M Lei Departments of Genetics, Cell Biology, and Development Search for other works by this author on: Oxford Academic Google Scholar C Frethem, C Frethem Departments of Genetics, Cell Biology, and Development Search for other works by this author on: Oxford Academic Google Scholar C L Wells C L Wells Departments of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis, 55455 Search for other works by this author on: Oxford Academic Google Scholar Microscopy and Microanalysis, Volume 9, Issue S02, 1 August 2003, Pages 250–251, https://doi.org/10.1017/S143192760344004X Published: 06 August 2003

10.1017/s143192760344004x

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Citations (1)