Miao Gen

Second Military Medical University

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i-10 index

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Co-Authors

Zhongtian Qi

Hebei Agricultural University

Haoran Peng

Naval University of Engineering

Cuiling Ding

Naval University of Engineering

Cui‐Ling Ding

Second Military Medical University

Yangang Liu

Naval University of Engineering

Peng Zhang

Center for Excellence in Molecular Plant Sciences

Bin Liu

Hormel (United States)

Xu Zheng

Institute of Infection and Immunity

Tingting Zhao

Shanghai Jiao Tong University

Wen Wang

Sichuan University

Cooperative Institutions

Army Medical University

Chinese PLA General Hospital

Second Military Medical University

Naval University of Engineering

Chinese People's Liberation Army

Chinese Academy of Sciences

Guangzhou Medical University

Chongqing Medical University

United States Department of the Navy

Shanxi Medical University

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Probability to produce animal vaccines in insect baculovirus expression system

AFRICAN JOURNAL OF BIOTECHNOLOGY (2011)

Roy Bhaskar Shuang Liang Peng Zhang Wang mei xian Zhou Fang

The insect baculovirus expression system is a valuable tool for the production of vaccine. Many subunit vaccines have been expressed in this system. The first vaccine produced in insect cells for animal use is now in the market. In this study, we reviewed recent progress of animal’s vaccine production for different expression levels and baculovirus genome stability, characteristic features of baculovirus expression vector system (BVES), virus link particles, baculovirus expression in mammalian cell and methodology of produce subunit vaccines. This review showed that BVES is a fantastic tool for vaccine development and it has wonderful feature for future animal vaccine development. Key words: Baculovirus expression system, vaccine, subunit vaccine, DNA vaccine.

10.5897/ajb09.192

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Citations (3)

Hepatoprotective effect of Apocynum venetum L extract on fatty liver disease of 2K1C rats with high-fat and refined-carbohydrate diet

Shijie huaren xiaohua zazhi (2009)

Yang Xin-bo Xiangqi Wu Yang Jie-ren Zheng-Ming Huang Hongyan Chen

10.11569/wcjd.v17.i2.135

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Citations (3)

Documentaries on research of coronavirus disease 2019(COVID-19) as of 11 March, 2020

Zhongguo renshougonghuanbing zazhi (2020)

Miao Gen CuiLing Ding Haoran Peng Zhongtian Qi

The first pneumonia case with unknown etiology appeared in Wuhan on December 1, 2019, and the pathogen of which was identified as a new species of coronavirus The World Health Organization (WHO) was tentatively named the virus as 2019 novel coronavirus (2019-nCoV) on January 31, 2020 Then the caused pneumonia was named as novel coronavirus pneumonia (NCP) by National Health Commission on February 8, 2020, which was changed to coronavirus disease 2019 (COVID-19) under agreed guidelines of WHO three days later on February 11, 2020 One same day, the International Committee on Taxonomy of Viruses designated the 2019-nCoV as severe acute respiratory syndrome coronavirus 2 (2019-nCoV) WHO characterizes COVID-19 as a pandemic on March 11, 2020 After the outbreak of COVID-19, researchers at home and abroad make great efforts in doing epidemiology investigation, establishing detection methods, improving clinical diagnosis and treatment plan, exploring pathogenesis and developing anti-viral drugs and vaccines, effectively preventing the spread of the domestic epidemic and winning time for controlling the epidemic outside China Based on the axis of time, this documentary article records the important events in research progress of COVID-19 in genomics, virus origins, epidemiology, detection, pathogenesis, clinical diagnosis and treatment

Pandemic

Coronavirus

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Advance in Zika virus infection-related myelitis

Zhonghua weishengwuxue he mianyixue zazhi (2019)

Miao Gen Cui‐Ling Ding Yangang Liu Zhongtian Qi

Zika virus belongs to the family Flaviviridae, genus Flavivirus and is mainly transmitted to humans by Aedes. The outbreak of Zika virus infection in South America in 2015 raised worldwide health concern due to the increasing incidence of microcephaly, Guillain-Barre syndrome and myelitis, although most of the patients were asymptomatic. Here, to further understand and elucidate the pathogenic mechanism of Zika virus infection-associated myelitis, this review summarized the latest advance in biological characteristics, transmission and treatment of Zika virus infection as well as the related case reports and possible mechanisms. Key words: Zika virus; Myelitis; Pathogenic mechanism

Zika Virus

Microcephaly

Flavivirus

10.3760/cma.j.issn.0254-5101.2019.10.013

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Identification of leukemia-associated antigens in chronic myeloid leukemia by proteomic analysis

Leukemia Research (2005)

Liyun Zou Yuzhang Wu Pei Li Daping Zhong Miao Gen

10.1016/j.leukres.2005.04.021

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Citations (38)

Development of a freezing drier for lyophilization of biomaterials

Journal of Biomedical Engineering (2004)

Zhigu Wu Miao Gen Tongzhu Sun Xiaobing Fu De-Xin Xiong

Biomaterial

Condenser (optics)

Strain (injury)

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[The effect of rosuvastatin therapy on CCR2 expression in mononuclear cells and its upstream pathway].

PubMed (2016)

Ruixue Du Ping Ye Guang‐Tao Yan Zihui Deng Wentao Liang

To investigate the effect of rosuvastatin therapy on C-C chemokine receptor(CCR2)expression in mononuclear cells in patients with carotid atherosclerosis and explore the possible upstream mechanism.Twenty patients without previous statin treatment were enrolled. Rosuvastatin were given 5 to 20 mg/day for 3 months. At baseline and 12 weeks, lipid profile and plasma monocyte chemotactic protein-1 (MCP-1) levels were examined. The mRNA and protein expressions of CCR2 in the mononuclear cells were measured with RT-PCR and flow cytometry, respectively. The mRNA and protein expression of peroxidase proliferator-activated receptor(PPAR β) were detected with RT-PCR and Western blot, respectively.After 3-months rosuvastatin treatment, the patients' low-density lipoprotein cholesterol (LDL-C) levels decreased significantly (P<0.01). Compared with baseline, the mRNA and protein expressions of CCR2 in the mononuclear cells showed significantly decrease, as well as plasma MCP-1 levels (P<0.05). Both mRNA and protein expressions of PPAR β in the mononuclear cells increased (P<0.05).Rosuvastatin may attenuate MCP-1/CCR2 through PPARβ upstream pathway.

CCR2

Monocyte

10.13459/j.cnki.cjap.2016.03.003

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Citations (1)

An application of multi-media technique in teaching of traditional Chinese painting

Journal of Changchun University (2003)

Miao Gen

Whith swift development of scientifical technology,the teaching of traditional Chinese painting is facing an urgent need of reforming.For a long period.only teachers teach in class.Hardly does it make students understand the traditional Chinese painting deeply without observing natural landscape and a great many social practices.It is obvious that the traditional teaching method has limition to improve the teaching quality.The article makes an analysis of role of the multimedia teaching in the educational reform and application of the multimedia technique in the teaching of the traditional Chinese painting.

Swift

Chinese painting

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Chikungunya virus and autoimmunity: Consensus immune epitope analysis between chikungunya virus and arthritis

Autoimmunity Reviews (2021)

Cui‐Ling Ding Miao Gen Yangang Liu Wen Wang Zhongtian Qi

10.1016/j.autrev.2021.102789

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Citations (2)

Antiviral efficacy of selective estrogen receptor modulators against SARS‐CoV‐2 infection in vitro and in vivo reveals bazedoxifene acetate as an entry inhibitor

Journal of Medical Virology (2022)

Miao Gen Haoran Peng Hailin Tang Yangang Liu Xu Zheng

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the seventh member of the coronavirus family that can infect humans. Recently, more contagious and pathogenic variants of SARS-CoV-2 have been continuously emerging. Clinical candidates with high efficacy and ready availability are still in urgent need. To identify potent anti-SARS-CoV-2 repurposing drugs, we evaluated the antiviral efficacy of 18 selective estrogen receptor modulators (SERMs) against SARS-CoV-2 infection. Six SERMs exhibited excellent anti-SARS-CoV-2 effects in Vero E6 cells and three human cell lines. Clomifene citrate, tamoxifen, toremifene citrate, and bazedoxifene acetate reduced the weight loss of hamsters challenged with SARS-CoV-2, and reduced hamster pulmonary viral load and interleukin-6 expression when assayed at 4 days postinfection. In particular, bazedoxifene acetate was identified to act on the penetration stage of the postattachment step via altering cholesterol distribution and endosome acidification. And, bazedoxifene acetate inhibited pseudoviruses infection of original SARS-CoV-2, Delta variant, Omicron variant, and SARS-CoV. These results offer critical information supporting bazedoxifene acetate as a promising agent against coronaviruses.

Toremifene

Vero cell

10.1002/jmv.27951

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Citations (9)