Hazem S. Elshafie

University of Basilicata

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Ippolito Camele

University of Basilicata

Vincenzo De Feo

University of Salerno

Laura De Martino

University of Salerno

Sadeek A. Sadeek

Zagazig University

Lucia Caputo

University of Salerno

ȘM

Ștefania Mirela Mang

University of Basilicata

Sabino Aurelio Bufo

University of Basilicata

Amira A. Mohamed

Higher Institute of Engineering

Adriano Sofo

University of Basilicata

Daniela Gruľová

University of Prešov

Cooperative Institutions

University of Basilicata

Zagazig University

University of Salerno

King Abdulaziz University

University of Prešov

University of Bari Aldo Moro

National Research Council

University of Naples Federico II

University of Palermo

University of Foggia

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Effects of different heat treatments on lysozyme quantity and antimicrobial activity of jenny milk

Journal of Dairy Science (2016)

Carlo Cosentino Cristiana Labella Hazem S. Elshafie Ippolito Camele Mauro Musto

Pasteurization

10.3168/jds.2015-10702

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Citations (39)

A Comprehensive Review on the Biological, Agricultural and Pharmaceutical Properties of Secondary Metabolites Based-Plant Origin

International Journal of Molecular Sciences (2023)

Hazem S. Elshafie Ippolito Camele Amira A. Mohamed

Natural products are compounds produced by living organisms and can be divided into two main categories: primary (PMs) and secondary metabolites (SMs). Plant PMs are crucial for plant growth and reproduction since they are directly involved in living cell processes, whereas plant SMs are organic substances directly involved in plant defense and resistance. SMs are divided into three main groups: terpenoids, phenolics and nitrogen-containing compounds. The SMs contain a variety of biological capabilities that can be used as flavoring agents, food additives, plant-disease control, strengthen plant defenses against herbivores and, additionally, it can help plant cells to be better adapted to the physiological stress response. The current review is mainly focusing on certain key elements related to the significance, biosynthesis, classification, biochemical characterization and medical/pharmaceutical uses of the major categories of plant SMs. In addition, the usefulness of SMs in controlling plant diseases, boosting plant resistance and as potential natural, safe, eco-friendly substitutes for chemosynthetic pesticides were also reported in this review.

Plant disease

10.3390/ijms24043266

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Citations (146)

Plant Essential Oil with Biological Activity

Plants (2022)

Hazem S. Elshafie

Plant essential oils (PEOs), extracted from many aromatic and medicinal plants, are used in folk medicine and often represent an important part of the traditional pharmacopoeia: they have a long history of use in folk medicine as antimicrobial agents to control several human and phyto-pathogens. Many PEOs have been registered as effective alternatives to chemical and synthetic antimicrobials, and in the last few decades, they have also been effectively used in the food industry as antioxidants and anticarcinogens, thanks to the efforts of many research/medical institutions and pharmaceutical companies. This Special Issue discussed the chemical composition and biological-pharmaceutical activities of some important PEOs and their single constituents. Detailed information has been also covered in this Special Issue regarding the mechanisms, possible modes of action, and factors affecting these activities, such as geographical origins, environmental conditions, nutritional status, and the extraction methods used.

Folk medicine

Pharmacopoeia

10.3390/plants11070980

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Biological investigations on the role of hydrogel formulations containing bioactive natural agents against some common phytopathogens of <em>Phaseolus vulgaris</em> L. and seed germination

Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale (2020)

Hazem S. Elshafie Maria Nuzzaci Giuseppina Logozzo Tania Gioia Ippolito Camele

Recent scientific research have manipulated the use of hydrogel in seed coating technology based on synthetic and chemical additives. The current study has been carried out to evaluate the biological activity of new seed coating formulations containing hydrogel based on natural substances or beneficial microorganisms on seed germination and controlling some common diseases of Phaseolus vulgaris L. New formulations have been prepared as single mixtures of hydrogel with the following bioactive substances: i) oregano Essential Oil (org EO); ii) Ornithine Lipid (OL); and two microorganisms i) Burkholderia gladioli and ii) Trichoderma harzianum T22. Results revealed that, the hydrogel formulation based org EO showed the highest significant activity against the majority of tested phytopathogens in a dose dependent manner. Regarding the antagonistic microbial activity, results showed that hydrogel formulations based T. harzianum T22 and B. gladioli were able to significantly reduce the growth of the majority of tested phytopathogens. In addition, the highest significant percentage of seed germination has been achieved using the formulations of B. gladioli and org EO. Regarding the disease incidence suppression assay, results explicated that org EO and OL were able to significantly inhibit the fungal disease incidence on P. vulgaris seeds steadily depending on the tested concentrations. In conclusion, the use of natural bioactive substances in hydrogel formulation would greatly reduce dependence on chemical pesticides and hence decreasing the environmental pollution and eventual harmful effects on plant, animal and human health.

Trichoderma harzianum

10.4081/jbr.2020.9219

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Evaluation of the Antimicrobial Activity of Four Plant Essential Oils against Some Food and Phytopathogens Isolated from Processed Meat Products in Egypt

Foods (2022)

Shahenda S. Elshafie Hazem S. Elshafie Rasha M. El Bayomi Ippolito Camele Alaa Eldin M.A. Morshdy

Synthetic preservatives are widely utilized by the food industry to inhibit the microbial contamination and increase food safety and shelf life. The excessive utilization of synthetic preservatives can have a negative impact on human health and the environment. There is a great interest to find out natural substances as possible food-preservatives. The consumers' preference for food products with natural ingredients prompted food manufacturers to utilize natural-based preservatives in their production. It is worth noting that plant essential oils (EOs) among the natural-based substances have been efficiently used as antimicrobial agents against phyto- and food pathogens. The current study was conducted to evaluate the microbial contamination of three industrial meat products from five governorates in Egypt, identify the predominant bacterial and fungal isolates and determine the antimicrobial efficacy of some EOs (thyme, fennel, anise and marjoram) against the most predominant microbial isolates. A sensory test was also performed to estimate the customer preferences for specific organoleptic aspects of meat products after EOs treatment. Results showed that there is a promising antimicrobial activity of all studied EOs against some microbial isolates in a dose-dependent manner. In particular, thyme EO showed the highest significant antibacterial activity against P.fluorescence and E. coli. Whereas the marjoram EO showed the highest activity against P. aeruginosa. In addition, the sensory test revealed that the treatment with anise and marjoram EOs showed the highest acceptability by the testers and did not show significant differences on the organoleptic properties with respect to control. As overall, the obtained results of the current research are promising and proved feasibility of employing plant EOs as possible preservatives for processed meat products.

Organoleptic

Food Preservatives

10.3390/foods11081159

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Citations (10)

Antimicrobial and Phytotoxic activity of essential oil of Origanum vulgare growing in Cilento

Teresa Della Pepa Hazem S. Elshafie Raffaele Capasso Vincenzo De Feo Laura De Martino

Origanum

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Investigating the Effects of Plant Essential Oils on Post-Harvest Fruit Decay

InTech eBooks (2016)

Hazem S. Elshafie Ippolito Camele

Essential oils are one of the most important natural products derived from plants, due to their various biological properties and their medicinal and nutritional uses. This chapter provides an overview of several different aspects relating to essential oils including a historical perspective, the uses of essential oils, their main sources and antifungal activity, their bioactive single constituents and their modes of action. The chapter will also give an insight into the chemical measures necessary for controlling plant pathogens and their negative impact on human health and/or the environment. It will also review the different sources of essential oils such as sage, oregano, thyme and marjoram from the Lamiaceae family, vervain from the Verbanacae family, and magnolia from the Magnoliaceae family. The antimicrobial activity of essential oils is reviewed, with particular emphasis on the antifungal properties exhibited against some serious pathogenic fungi and post-harvest disease. Moreover, various antimicrobial tests and techniques, such as various kill-time studies, killing time determination, LD-50 and growth curve recording, poisoned food techniques, spore germination and measurement of metabolic CO2 are included. Finally, five case studies relating to the antifungal activity of some plant essential oils, either in vitro or in vivo, against post-harvest pathogenic fungi are reviewed at the end of this chapter.

Magnoliaceae

Spore germination

Aromatic plants

Fungal growth

10.5772/62568

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Citations (11)

Antimicrobial Activity and Chemical Composition of Essential Oil Extracted from Solidago canadensis L. Growing Wild in Slovakia

Molecules (2019)

Hazem S. Elshafie Daniela Gruľová Beata Baranová Lucia Caputo Laura De Martino

Plant essential oils (EOs) are one of the most relevant natural products due to their biological, medicinal, and nutritional properties. The promising biological effects of many plants EOs encourage researchers to study their biochemical properties to be used as possible natural alternatives for commercial pesticides and not only as herbal medicines. The current research has been conducted to study the microbicide effect of Solidago canadensis L. EO to control some common plant diseases caused by several postharvest phytopathogenic fungi (Monilinia fructicola, Botrytis cinerea, Aspergillus niger, and Penicillium expansum) in comparison with Azoxystrobin as a large spectrum fungicide. The antibacterial activity has been carried out against some phytopathogenic bacteria (Bacillus megaterium and Clavibacter michiganensis (G+ve) and Xanthomonas campestris, Pseudomonas fluorescens, and Pseudomonas syringae pv. phaseolicola (G-ve)) compared to the synthetic antibiotic Tetracycline. Minimum inhibitory concentration was carried out to determine the lowest effective EO dose using a 96-well microplate. The cell membrane permeability was also evaluated by measuring the electric conductivity (EC) to examine the possible mechanisms of action of S. canadensis EO. Chemical characterization of EO has been carried out using gas chromatography and mass spectrometry (GC-MS). Thirty-two identified components in S. canadensis EO presented 97.7% of total compounds in EO. The principal compounds were identified as germacrene D (34.9%), limonene (12.5%), α-pinene (11.6%), β-elemene (7.1%), and bornyl acetate (6.3%). In addition, S. canadensis EO demonstrated promising in vitro antimicrobial activities against the majority of tested phytopathogens at all tested concentrations.

Clavibacter michiganensis

Monilinia fructicola

Pseudomonas fluorescens

10.3390/molecules24071206

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Citations (71)

Chemical composition and antimicrobial activity of chia (Salvia hispanica L.) essential oil

European Food Research and Technology (2018)

Hazem S. Elshafie Luigi Aliberti Mariana Amato Vincenzo De Feo Ippolito Camele

Salvia

10.1007/s00217-018-3080-x

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