Serge Rezzi

Nestlé (Switzerland)

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François‐Pierre Martin

Nestlé (Switzerland)

Sunil Kochhar

University of Delhi

Sebastiano Collino

Nestlé (Switzerland)

Ivan Montoliu

Nestlé (Switzerland)

Philippe A. Guy

Nestlé (Switzerland)

Jeremy K. Nicholson

Imperial College London

Stéphane Canarelli

École Polytechnique Fédérale de Lausanne

Alastair B. Ross

AgResearch

Elaine Holmes

Imperial College London

Claude Guillou

Joint Research Centre

Cooperative Institutions

Nestlé (Switzerland)

133

University of Lausanne

École Polytechnique Fédérale de Lausanne

Nestlé (Brazil)

Imperial College London

Centre National de la Recherche Scientifique

University Hospital of Lausanne

Bioanalytica (Switzerland)

University of Bern

Inserm

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First identification of dimethoxycinnamic acids in human plasma after coffee intake by liquid chromatography–mass spectrometry

Journal of Chromatography A (2010)

Kornél Nagy Karine Redeuil Gary Williamson Serge Rezzi Fabiola Dionisi

10.1016/j.chroma.2010.11.076

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Citations (43)

Quantitative Profiling of Endogenous Fat-Soluble Vitamins and Carotenoids in Human Plasma Using an Improved UHPSFC-ESI-MS Interface

Analytical Chemistry (2017)

Filomena Petruzziello Alexandre Grand‐Guillaume Perrenoud Anita Thorimbert Michael Fogwill Serge Rezzi

Analytical solutions enabling the quantification of circulating levels of liposoluble micronutrients such as vitamins and carotenoids are currently limited to either single or a reduced panel of analytes. The requirement to use multiple approaches hampers the investigation of the biological variability on a large number of samples in a time and cost efficient manner. With the goal to develop high-throughput and robust quantitative methods for the profiling of micronutrients in human plasma, we introduce a novel, validated workflow for the determination of 14 fat-soluble vitamins and carotenoids in a single run. Automated supported liquid extraction was optimized and implemented to simultaneously parallelize 48 samples in 1 h, and the analytes were measured using ultrahigh-performance supercritical fluid chromatography coupled to tandem mass spectrometry in less than 8 min. An improved mass spectrometry interface hardware was built up to minimize the post-decompression volume and to allow better control of the chromatographic effluent density on its route toward and into the ion source. In addition, a specific make-up solvent condition was developed to ensure both analytes and matrix constituents solubility after mobile phase decompression. The optimized interface resulted in improved spray plume stability and conserved matrix compounds solubility leading to enhanced hyphenation robustness while ensuring both suitable analytical repeatability and improved the detection sensitivity. The overall developed methodology gives recoveries within 85–115%, as well as within and between-day coefficient of variation of 2 and 14%, respectively.

Repeatability

10.1021/acs.analchem.7b01476

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Citations (48)

Nutritional Metabonomics: An Approach to Promote Personalized Health and Wellness

CHIMIA International Journal for Chemistry (2011)

Sebastiano Collino François‐Pierre Martin Sunil Kochhar Serge Rezzi

Nutritional research has emerged in the last century from the study of nutrients as a means of nourishment to the general population to the quest for wellness improvement through specific food components. Advances in nutrigenomics technologies have allowed nutrition scientists to be for the first time at the forefront of nutritional research. Such advances have given them the ability to discern new vital scientific discoveries specifically for the development of new tailored dietary patterns. In this, nutritional metabonomics has rapidly evolved into a very powerful bioanalytical tool able to assess multi-parametric metabolic responses of living organisms to specific dietary interventions. Nutritional metabonomics therefore provides a systematic approach through the comprehensive analysis of metabolites aiming today at the quest for homeostatic balance which is dependent not only on the host but also on the crucial metabolic interactions with microbial symbionts.

Nutrigenomics

10.2533/chimia.2011.396

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Citations (11)

Promoting Gut Health with Probiotic Metabolomics

Springer eBooks (2011)

Sebastiano Collino François‐Pierre Martin Sunil Kochhar Serge Rezzi

10.1007/978-94-007-0386-5_8

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Citations (0)

Application of support vector machines to 1H NMR data of fish oils: methodology for the confirmation of wild and farmed salmon and their origins

Analytical and Bioanalytical Chemistry (2007)

Saeed Masoum Christophe Malabat Mehdi Jalali‐Heravi Claude Guillou Serge Rezzi

Misrepresentation

10.1007/s00216-006-1025-x

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Citations (60)

Classification of Gilthead Sea Bream (Sparus aurata) from ¹H NMR Lipid Profiling Combined with Principal Component and Linear Discriminant Analysis

Journal of Agricultural and Food Chemistry (2007)

Serge Rezzi I. Giani Károly Héberger David E. Axelson V.M. Moretti

The combination of 1H NMR fingerprinting of lipids from gilthead sea bream (Sparus aurata) with nonsupervised and supervised multivariate analysis was applied to differentiate wild and farmed fish and to classify farmed specimen according to their areas of production belonging to the Mediterranean basin. Principal component analysis (PCA) applied on processed 1H NMR profiles made a clear distinction between wild and farmed samples. Linear discriminant analysis (LDA) allowed classification of samples according to the geographic origin, as well as for the wild and farmed status using both PCA scores and NMR data as variables. Variable selection for LDA was achieved with forward selection (stepwise) with a predefined 5% error level. The methods allowed the classification of 100% of the samples according to their wild and farmed status and 85–97% to geographic origin. Probabilistic neural network (PNN) analyses provided complementary means for the successful discrimination among classes investigated.

Profiling (computer programming)

10.1021/jf070736g

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Citations (59)

Topographical Body Fat Distribution Links to Amino Acid and Lipid Metabolism in Healthy Non-Obese Women

PLoS ONE (2013)

François‐Pierre Martin Ivan Montoliu Sebastiano Collino Max Scherer Philippe A. Guy

Visceral adiposity is increasingly recognized as a key condition for the development of obesity related disorders, with the ratio between visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) reported as the best correlate of cardiometabolic risk. In this study, using a cohort of 40 obese females (age: 25-45 y, BMI: 28-40 kg/m(2)) under healthy clinical conditions and monitored over a 2 weeks period we examined the relationships between different body composition parameters, estimates of visceral adiposity and blood/urine metabolic profiles. Metabonomics and lipidomics analysis of blood plasma and urine were employed in combination with in vivo quantitation of body composition and abdominal fat distribution using iDXA and computerized tomography. Of the various visceral fat estimates, VAT/SAT and VAT/total abdominal fat ratios exhibited significant associations with regio-specific body lean and fat composition. The integration of these visceral fat estimates with metabolic profiles of blood and urine described a distinct amino acid, diacyl and ether phospholipid phenotype in women with higher visceral fat. Metabolites important in predicting visceral fat adiposity as assessed by Random forest analysis highlighted 7 most robust markers, including tyrosine, glutamine, PC-O 44∶6, PC-O 44∶4, PC-O 42∶4, PC-O 40∶4, and PC-O 40∶3 lipid species. Unexpectedly, the visceral fat associated inflammatory profiles were shown to be highly influenced by inter-days and between-subject variations. Nevertheless, the visceral fat associated amino acid and lipid signature is proposed to be further validated for future patient stratification and cardiometabolic health diagnostics.

Intra-Abdominal Fat

10.1371/journal.pone.0073445

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Citations (43)

Quantification of alkylresorcinols in human plasma by liquid chromatography/tandem mass spectrometry

Rapid Communications in Mass Spectrometry (2010)

Alastair B. Ross Karine Redeuil Mario Vigo Serge Rezzi Kornél Nagy

Alkylresorcinols (AR) are of interest as biomarkers of wholegrain wheat and rye intake in epidemiological studies and are currently mainly measured by gas chromatography/mass spectrometry (GC/MS) after labour-intensive sample preparation including liquid-liquid extraction, solid-phase extraction (SPE) and chemical derivatization. This manuscript describes and validates an alternative approach based on normal-phase liquid chromatography/tandem mass spectrometry for the quantification of alkylresorcinols in human plasma. The method requires neither SPE nor chemical derivatization and has a shortened run time compared to GC/MS. Normal- and reversed-phase columns and various mobile phases were evaluated with and without previous SPE of the samples. Normal-phase chromatography allowed separation of AR from the interfering triacylglycerols, diacylglycerols and sterols and enabled detection of AR even without SPE of the samples. The described method has instrumental lower limits of detection in the 25-75 pg range, and lower limits of quantification in the 75-250 pg range. Pooled human plasma and (2)H(4)-nonadecylresorcinol (internal standard) was applied to calibrate the method in the 20-12 000 nM range. The overall method showed intra-batch precision of 8.6% and an averaged accuracy of 100.2%. Applications for diverse human plasma samples are presented and are compared with the results determined by GC/MS. Based on the presented data; this method requiring less sample preparation is suggested for further evaluation as an alternative to GC/MS for analysis of biomarkers of wholegrain wheat and rye intake in epidemiological studies.

Solid phase extraction

Sample Preparation

10.1002/rcm.4409

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Citations (25)

Metabotyping of Biofluids Reveals Stress-Based Differences in Gut Permeability in Healthy Individuals

Journal of Proteome Research (2009)

Serge Rezzi François‐Pierre Martin Carmen Alonso Mar Guilarte María Vicario

Life stress promotes gut dysfunction, but underlying biochemical events are not well-understood. In the present study, we describe the metabolic events associated with background stress and its potential influence on the response to novel incoming stress stimulus in healthy subjects. A 15 min cold pain test was carried out in healthy men and women stratified according to low (LS; n = 21) and moderate background stress (MS; n = 9) during jejunal segmental perfusion. Cold pain induced a stronger psychological response in MS compared to LS subjects, but similar increases in heart rate and blood pressure. Urine and plasma were analyzed using 1H NMR-based metabonomics. Basal stress levels were imprinted in the metabolic profiles indicating different energy and lipid homeostasis. Cold pain increased gut permeability, as determined by mannitol and xylose levels, the response being greater in LS subjects. Moreover, the rate of plasma clearance of mannitol and xylose was dependent on background stress level and gender. In addition, cold pain modulated the levels of circulating ketone bodies, Krebs's cycle intermediates, glucose, and the glucogenic alanine and lactate, which brings further evidence of an alteration of energy homeostasis. Therefore, unravelling life stress with metabonomics may provide a system biology basis for the clinical management and therapeutic surveillance of gut health and disorders.

Homeostasis

Intestinal Permeability

10.1021/pr900525w

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Citations (32)

High Prevalence of Hypovitaminosis D in Adolescents Attending a Reference Centre for the Treatment of Obesity in Switzerland

Children (2022)

Pollyanna Patriota Sylvie Borloz Inge Ruiz Thérèse Bouthors Serge Rezzi

Background: Hypovitaminosis D is common in populations with obesity. This study aimed at assessing (1) the prevalence of hypovitaminosis D and (2) the associations between vitamin D levels and cardiovascular risk factors in adolescents attending a reference centre for the treatment of obesity. Design: Cross-sectional pilot study conducted in the paediatric obesity unit of the Lausanne university hospital, Switzerland. Methods: Participants were considered eligible if they (1) were aged between 10 to 16.9 years and (2) consulted between 2017 and 2021. Participants were excluded if (1) they lacked vitamin D measurements or (2) the vitamin D measurement was performed one month after the base anthropometric assessment. Hypovitaminosis D was considered if the vitamin D level was <30 ng/mL (<75 nmol/L). Severe obesity was defined as a BMI z-score > 3 SD. Results: We included 52 adolescents (31% girls, mean age 13 ± 2 years, 33% with severe obesity). The prevalence of hypovitaminosis D was 87.5% in girls and 88.9% in boys. The vitamin D levels were inversely associated with BMI, Spearman r and 95% CI: −0.286 (−0.555; −0.017), p = 0.037; they were not associated with the BMI z-score: −0.052 (−0.327; 0.224), p = 0.713. The vitamin D levels were negatively associated with the parathormone levels (−0.353 (−0.667; −0.039), p = 0.028) and positively associated with the calcium levels (0.385 (0.061; 0.708), p = 0.020), while no association was found between vitamin D levels and blood pressure and lipid or glucose levels. Conclusion: almost 9 out of 10 adolescents with obesity in our cohort presented with hypovitaminosis D. Hypovitaminosis D does not seem to be associated with a higher cardiovascular risk profile in this group.

Hypovitaminosis

Cross-sectional study

10.3390/children9101527

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Citations (2)