VI Congreso de la Sociedad Espanola de Biologia Evolutiva (SESBE) - 6th Meeting of the Spanish Society for Evolutionary Biology (SESBE), 17-19 January 2018, Palma.-- 1 page
Abstract Global warming is an environmental phenomenon to which species must adapt to survive. Drosophila subobscura presents an adaptive capacity due to its chromosomal inversion polymorphism. Until now, the impact of global warming on this polymorphism has been studied in D. subobscura populations located either on a continental mainland or on islands not far from a continent. In this context, gene flow could be a relevant mechanism allowing the movement of thermally adapted inversions between populations. Our aim was to sample and study the chromosomal polymorphism on Madeira, a small isolated island in the Atlantic Ocean. We compared our findings with those reported in the same location approximately four and five decades ago. Moreover, we studied whether global warming has occurred on this island by analyzing mean, maximum and minimum temperatures over a 55‐year period. All atmospheric parameters have increased significantly, consistent with climate change expectations. Frequencies and chromosomal thermal index values of thermal adapted inversions remained quite stable over years. Furthermore, J, U and O chromosomes are almost fixed for “warm” adapted inversions. Thus, if there is little genetic variability remaining and temperatures continue increasing, island populations of D. subobscura might be on the threshold of endangerment. However, apart from selection, genetic drift and inbreeding, other processes, such as phenotypic plasticity or thermoregulatory behavior, could be involved in the survival of the species’ populations. Finally, although in danger, D. subobscura is a generalist that lives in humanized environments, and this fact could favor its persistence on Madeira Island.
In this work, the process of colonization of North and South America by the species Drosophila subobscura has been studied by analyzing the variability of lethal genes. The genetic structures of a Palearctic natural central population (Bordils, Spain) and a colonizer population from America (Gilroy, California) have been compared. The frequencies of lethal chromosomes and their allelism are 29.007% and 0.0069 in the first population and 14.414% and 0.0526 in the American population. A founder effect is detected after the computation of some population parameters (Ne , h, he and the lethal load). Furthermore, the allelism of lethal chromosomes has revealed a strong association between a lethal gene and the O5 inversion both in Gilroy and in the population of Puerto Montt (Chile). The interpopulation allelism shows that the O5 arrangement from the USA and Chile is the same, confirming that the colonizing processes of North and South America are correlated. The O5 arrangement can also be useful as a genetic marker to trace the origin of the colonization. The frequency of the O5 arrangement in the original population of the colonization could be used to estimate the number of colonizers. This population is still unknown, but taking the extreme values of the frequency of the O5 inversion in natural Palearctic populations (1-15%), the number of colonizers could vary between 9 and 149 individuals.
espanolLa justicia y la ciencia son ambitos muy diferentes de la actividad humana. Sin embargo, los conocimientos cientificos dan lugar a la aparicion de una serie de disciplinas aplicadas que son de gran utilidad en forensia y que pueden ser trascendentales en la Administracion de la Justicia. El problema es reconocer primero si una especialidad es realmente cientifica y si puede utilizarse de manera fiable en el ambito de la justicia. En este sentido, la utilizacion de las pruebas de ADN es un buen ejemplo de como, poco a poco, se ha ido ajustando su uso en los tribunales. Ademas los laboratorios donde se practican esas tecnicas forenses estan correctamente homologados y al mismo tiempo los especialistas que trabajan en ellos son profesionales con una solida formacion y que se mantienen constantemente al dia. Otro elemento crucial es que los expertos que aportan su testimonio deben hacerlo de manera precisa y comprensible. En los casos en que se presente una prueba pericial basada en una aproximacion cientifica novedosa, esta debe cumplir toda una serie de requisitos para poder ser aceptada por los tribunales. EnglishJustice and science are very different human activities. However, scientific knowledge has generated many disciplines that are useful in forensics and could be key elements to solve judicial cases. The first problem is to know whether a specialty is actually science and then, if it is enough reliable for being used in courtrooms. In this sense, DNA evidence is a good example of how its presence in the courts has been improved. Furthermore, laboratories where forensic analyses are carried out are properly accredited. Practitioners that work in them are professionals with an appropriate training that also has to be a continuous process. Finally, experts that present their testimony in the court have to be clear and understandable in their explanations. If they have to present a novel scientific approach as evidence, this must observe a series of requirements in order to be accepted by the court.
The phenotypic plasticity of some quantitative traits of two colonizing populations of Drosophila subobscura (Davis and Eureka, California) was studied. Temperature effects and the effect of rearing in the laboratory were studied. Laboratory rearing during four generations at 18ºC significantly increased the wing and tibial length. This increase was similar to that obtained when the flies were reared at 13ºC during two generations.The low temperature environment can be considered more stressful for females than for males, as shown by the increase of phenotypic variance. The two populations analyzed had great phenotypic plasticity in spite of the genetic bottleneck during the colonization event. Our study shows that keeping flies for a relatively short time in the laboratory significantly changes some quantitative traits, emphasizing the need to analyze flies immediately after collecting them in order to obtain reliable estimates for the analysis of these traits in natural populations.