A Román

Universidade de Santiago de Compostela

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Sergio Vidal

Hospital Universitari i Politècnic La Fe

Lucas Moya

University of Münster

E. Prieto

Michigan State University

Dolores Subirá

Hospital Universitario de Guadalajara

Pablo Sánchez‐Quinteiro

Universidade de Santiago de Compostela

J Outeiriño

Hospital Universitario Fundación Jiménez Díaz

T Calabuig

Universidad Autónoma de Madrid

Eva Arranz

Hospital Universitario de La Princesa

Sánchez Fayos J

Hospital Universitario Fundación Jiménez Díaz

Pilar Llamas

Hospital Universitario Fundación Jiménez Díaz

Cooperative Institutions

Hospital Universitario Fundación Jiménez Díaz

Universidade de Santiago de Compostela

Universidad Autónoma de Madrid

Universidad de Salamanca

Universidad Complutense de Madrid

Universidade de Vigo

Hospital Clínico San Carlos

Instituto de Salud Carlos III

Centro de Investigación Biomédica en Red

Consejo Superior de Investigaciones Científicas

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Immunohistochemical Identification and Morphometric Study of ACTH Cells of Mink (Mustela vison) during Growth and Different Stages of Sexual Activity in the Adult

General and Comparative Endocrinology (1995)

Sergio Vidal A Román Lucas Moya

Identification

American mink

10.1006/gcen.1995.1127

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Citations (9)

Immunocytochemical Study of the Growth Hormone and Prolactin Pituitary Cells in Male and Female Suckling Mink

General and Comparative Endocrinology (1994)

Sergio Vidal Pablo Sánchez‐Quinteiro A Román Lucas Moya

Immunogold labelling

Fixative

Prolactin cell

Organelle

10.1006/gcen.1994.1038

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Citations (6)

[Essential thrombocythemia: a myeloproliferative state on the rise. Clinico-biological study and course of 44 cases].

PubMed (1992)

Sánchez Fayos J J Outeiriño E Prieto Pérez Sáenz T Calabuig

Forty-four cases of essential thrombocytosis (ET) were diagnosed in the last 20 years, 19 males and 24 females (M/F: 0.76), aged between 3 and 86 years (median, 62 years), and 9 of them being under 40 years of age. The M/F ratio for patients under 60 years was 0.5, whereas it was 1.09 for patients over 60. The clinical forms at onset were: asymptomatic, 36.5%; as a bleeding disorder (BD), 20.4%; as thrombotic disease (TD) 22.7%; BD/TD, 13.6%, and others, 6.8%. The most important biological features included platelet count over 1.000 x 10(9)/L (59.1%), abnormal platelet aggregation, chiefly with ADR (56.5), mild reticulin myelofibrosis (55%), abnormal karyotype (2.6%), moderately high LDH levels (56.8%) and pseudo-hyperkalaemia (40%). The initial therapeutic approach was: observation (12 cases), antiaggregating agents (6 cases), and chemotherapy (BSF, HU, etc.) in the remainders. One patient evolved quickly into acute myelogenous leukaemia and two others suffered a late transformation into polycythaemia vera (PV) and myeloid metaplasia, respectively. The median survival was over 11 years, this being longer in patients under 60 years of age, in those with platelet count at diagnosis between 600 and 1000 x 10(9)/L and in those without initial symptoms of thrombosis. The advent of electronic blood-cell counters has made ET no longer a rare chronic myeloproliferative disease, its incidence coming now closer to that of PV; thus, in the last four quinquennial periods the incidence of ET/PV has evolved as following: 1/19, 4/16, 13/18 and 26/29.

Polycythaemia

Thrombocytosis

Anagrelide

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Advantages of flow cytometry immunophenotyping for the diagnosis of central nervous system non‐Hodgkin's lymphoma in AIDS patients

HIV Medicine (2005)

Dolores Subirá Miguel Górgolas Susana Castañón Cristina Serrano A Román

Background Neurological disorders are common in HIV‐infected patients. Central nervous system (CNS) lymphoma should always be considered because it is an important cause of morbidity and mortality. Objectives To investigate the clinical utility of flow cytometry immunophenotyping (FCI) in diagnosing or discarding leptomeningeal involvement in HIV‐infected patients and to compare its sensitivity with that of conventional cytological methods. Methods Fifty‐six cerebrospinal fluid (CSF) samples from 29 HIV‐infected patients were independently evaluated by flow cytometry and cytology. The description of an aberrant immunophenotype was the criterion used to define the malignant nature of any CSF cell population. Results FCI and cytology gave concordant results for 48 of the 56 CSF samples studied: 37 were negative for malignancy and 11 had evidence of CNS lymphoma. Discordant results were obtained for eight CSF samples, and the accuracy of the FCI findings could be demonstrated for four CSF samples described as positive for malignancy according to the FCI criteria. Conclusions A high level of agreement was found between the results obtained using the two methods, but FCI gave at least 25% higher sensitivity than conventional cytomorphological methods for the detection of malignant cells. This advantage suggests that, in case of negative flow cytometry results, disorders other than non‐Hodgkin's lymphoma should be strongly considered.

Immunophenotyping

10.1111/j.1468-1293.2005.00260.x

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Citations (26)

Cytogenetic and molecular studies of siblings with ataxia telangiectasia followed for 7 years

Cancer Genetics and Cytogenetics (1997)

Mónica Renedo Mercedes Robledo Eva Arranz Fernando Infantes A Román

10.1016/s0165-4608(96)00256-7

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Citations (3)

Significance of the volume of fetomaternal hemorrhage after performing prenatal invasive tests

Cytometry Part B Clinical Cytometry (2010)

Dolores Subirá Montserrat Uriel Chora Serrano Susana Castañón Raquel Gonzalo

Background: Fetal erythrocytes cross the placenta during gestation, but invasive prenatal procedures might develop into fetomaternal hemorrhage (FMH). We examine whether flow cytometry immunophenotyping might be useful for measuring the volume of FMH after such procedures. Methods: Fetal erythrocytes (%) were determined in 153 pregnant women after amniocentesis (129) and chorionic villous sampling (24) using a monoclonal antibody against fetal hemoglobin. Fetal erythrocytes were identified for their high expression of fetal hemoglobin (HbF++). Blood samples from two control groups, 53 healthy males and 21 pregnant women not submitted to invasive tests, were used to establish normal values of circulating HbF++ erythrocytes in adults. Results: The highest percentage of HbF++ erythrocytes in the control groups was 0.015%. The rate of HbF++ erythrocytes in samples after invasive tests ranged between <0.01% and 0.15%. Seventy-three women (47%) had ≤0.015% HbF++ erythrocytes, and this rate was higher in 80. Nine women presented >1 ml of FMH (volume of packed cells corresponding to 0.054–0.15% HbF++ erythrocytes), but only two had sonographic evidence of bleeding. Conclusions: Most women in our series had a very low volume of FMH after the invasive tests. Acute bleeding should be thoroughly investigated in women with either more than 1 ml of packed cells or more than 0.05% of HbF++ erythrocytes. Intermediate values between >0.015% and <0.05%, should be carefully considered depending on the week of gestation. Data obtained before 15 weeks might reflect previous cell trafficking between fetus and mother instead of acute hemorrhage. © 2010 International Clinical Cytometry Society

Amniocentesis

Chorionic villus sampling

10.1002/cyto.b.20548

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Citations (17)

Response of the Fetal Pituitary-Thiroid Axis (FPTA) To different Doses of Thyrotropin-Releasing Hormone (Trh)

Pediatric Research (1997)

J Torres-Pereyra Fernando Moya R. Salinas Andrés D. Maturana Jeannie Poblete

10.1203/00006450-199701000-00025

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Citations (0)

118 Cytogenetic study and prognostic impact of complex karyotypes in 22 myelodysplastic syndromes